ABSTRACT
EXPERIMENTAL: and theoretical studies were conducted to investigate low and non-linear responses in sub-micro molar-level suppressed ion chromatography with a hydroxide eluent. A calculated response was derived using experimentally determined detector effluent ion composition data and compared with measured experimental responses. The calibration curve was non-linear, and its slope varied considerably with different instrumental setups and operating conditions. The non-linearity of the solution conductivity response was determined by two acid-base equilibria of water and carbonic acid, and fluoride ion. By using eluent contaminated with para-toluene sulfonate at micro molar level, the non-linear response was greatly alleviated.
Subject(s)
Chromatography, Ion Exchange/methods , Hydroxides/analysis , Benzenesulfonates/chemistry , Calibration , Carbonic Acid/chemistry , Chromatography, Ion Exchange/standards , Fluorides/chemistry , Hydroxides/isolation & purification , Hydroxides/standards , Limit of Detection , Water/chemistrySubject(s)
Hydroxides/standards , Indicators and Reagents/standards , Potassium Compounds/standards , Saline Solution , Sarcoptes scabiei , Scabies/diagnosis , Adult , Animals , Humans , Immunocompetence , Male , Penis/pathology , Pruritus , Scabies/parasitology , Scrotum/pathology , Skin/parasitology , Skin/pathologyABSTRACT
An opaque, water-insoluble quality control material with a skinlike microscopic appearance was prepared by inoculating melted xanthine (0.4%) agar with filamentous fungi and dispensing drops onto glass slides. After solidification of the agar, the material was rapidly cleared by 10% KOH, revealing fungal elements stained by Cellufluor reagent.
