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1.
Eur Arch Otorhinolaryngol ; 277(2): 611-621, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31792655

ABSTRACT

PURPOSE: The long non-coding RNA MALAT1 is a predictive marker in several solid tumors with highly conserved sequences. However, the role of non-coding RNA in development of laryngeal or hypopharyngeal cancer remains unclear. METHODS: Tumor tissues and adjacent non-cancer tissues of 24 patients were collected. We detected the expression of MALAT1 in laryngeal cancer tissues and hypopharyngeal cancer tissues. Moreover, we developed a MALAT1 silencing model in human laryngeal tumor cells by transfecting MALAT1 small interfering RNA into human laryngeal carcinoma cell line Hep-2 and pharyngeal carcinoma cell line FaDu with Lipofectamine 2000 system. Cell cycle analysis, Cell Counting Kit-8 assay, Transwell assay, quantitative reverse transcription PCR, and wound-healing assays were performed to evaluate the impact of MALAT1 depletion on laryngeal or hypopharyngeal cancer cell's growth, proliferation, apoptosis, invasion and migration. RESULTS: MALAT1 was significantly up-regulated in laryngeal and hypopharyngeal carcinoma cells. MALAT1 down-regulation induced the increased apoptosis of both cell lines and suppressed cells' proliferation. Cells were arrested in G1/G2 phase and cells of S phase were significantly decreased. Down-regulation of MALAT1 expression can also inhibit the migration and invasion of laryngeal squamous cell carcinoma cell (Hep-2) and hypopharyngeal cancer cell (FaDu). CONCLUSION: In summary, our deactivation model of MALAT1 disentangled the active function of it as a regulator of gene expression governing the hallmarks of laryngeal and hypopharyngeal cancer. Blocking this long non-coding RNA may restrain the development of laryngeal cancer.


Subject(s)
Carcinoma, Squamous Cell/genetics , Hypopharyngeal Neoplasms/genetics , Laryngeal Neoplasms/genetics , RNA, Long Noncoding/antagonists & inhibitors , RNA, Long Noncoding/genetics , Adult , Aged , Apoptosis/genetics , Biomarkers, Tumor/genetics , Carcinoma, Squamous Cell/metabolism , Carcinoma, Squamous Cell/prevention & control , Cell Movement/genetics , Cell Proliferation/genetics , Female , Gene Expression Regulation, Neoplastic/genetics , Gene Knockdown Techniques , Humans , Hypopharyngeal Neoplasms/prevention & control , Hypopharyngeal Neoplasms/surgery , Laryngeal Neoplasms/prevention & control , Laryngeal Neoplasms/surgery , Male , Middle Aged , Prognosis , RNA, Long Noncoding/biosynthesis , RNA, Small Interfering/genetics
2.
Am J Clin Nutr ; 102(2): 420-32, 2015 Aug.
Article in English | MEDLINE | ID: mdl-26156734

ABSTRACT

BACKGROUND: Head and neck cancer (HNC) is the seventh most-common type of cancer worldwide. Evidence regarding the potential protective effect of vitamins and carotenoids on HNC is limited and mostly based on case-control studies. OBJECTIVE: We evaluated the association of intake of dietary vitamins C and E (including supplementation) and the most-common carotenoids (α-carotene, ß-carotene, lutein plus zeaxanthin, lycopene, and ß-cryptoxanthin) and risk of HNC and HNC subtypes in a large prospective study. DESIGN: The Netherlands Cohort Study included 120,852 participants. For efficiency reasons, a case-cohort design was used. At baseline in 1986, participants completed a food-frequency questionnaire. A subcohort was randomly selected from the total cohort. After 20.3 y of follow-up, 3898 subcohort members and 415 HNC cases [131 oral cavity cancer (OCCs), 88 oro-/hypopharyngeal cancer (OHPs), and 193 laryngeal cancer cases] were available for analysis. Rate ratios and 95% CIs for highest (quartile 4) compared with lowest (quartile 1) quartiles of vitamin and carotenoid intake were estimated by using the Cox proportional hazards model. RESULTS: A strong inverse association was shown between vitamin C and HNC overall (multivariable-adjusted rate ratio for quartile 4 compared with quartile 1: 0.39; 95% CI: 0.23, 0.66; P-trend < 0.001), OCC (multivariable-adjusted rate ratio for quartile 4 compared with quartile 1: 0.35; 95% CI: 0.16, 0.77; P-trend < 0.05), and OHPC (multivariable-adjusted rate ratio for quartile 4 compared with quartile 1: 0.29; 95% CI: 0.12, 0.67; P-trend < 0.01). No statistically significant results were shown for vitamin E, α-carotene, ß-carotene, lycopene, and lutein plus zeaxanthin. The association of vitamin E and HNC was modified by alcohol status (P-interaction = 0.003) with lower risks in alcohol abstainers. CONCLUSIONS: With this study, we show an inverse association between intake of vitamin C and the incidence of HNC and HNC-subtypes. Future research is recommended to investigate the underlying mechanisms and to confirm our results, which may be promising for the prevention of HNC.


Subject(s)
Antioxidants/therapeutic use , Ascorbic Acid/therapeutic use , Diet , Dietary Supplements , Head and Neck Neoplasms/prevention & control , Aged , Antioxidants/administration & dosage , Ascorbic Acid/administration & dosage , Carotenoids/administration & dosage , Carotenoids/therapeutic use , Case-Control Studies , Cohort Studies , Female , Follow-Up Studies , Head and Neck Neoplasms/epidemiology , Humans , Hypopharyngeal Neoplasms/epidemiology , Hypopharyngeal Neoplasms/prevention & control , Incidence , Laryngeal Neoplasms/epidemiology , Laryngeal Neoplasms/prevention & control , Male , Middle Aged , Mouth Neoplasms/epidemiology , Mouth Neoplasms/prevention & control , Netherlands/epidemiology , Oropharyngeal Neoplasms/epidemiology , Oropharyngeal Neoplasms/prevention & control , Prospective Studies , Registries , Risk Factors , Vitamin E/administration & dosage , Vitamin E/therapeutic use
3.
Head Neck ; 34(2): 155-61, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21322082

ABSTRACT

BACKGROUND: We evaluated the effectiveness of narrow band imaging (NBI) in patients with oral squamous cell carcinoma (OSCC) after treatment. METHODS: In all, 101 consecutive OSCC patients underwent NBI examination for posttreatment follow-up. Four patients had local recurrence. Twenty-six second primary malignancies were found in 18 patients; 6 patients (33%) had more than 1 lesion. Seventeen lesions (65%) were carcinoma in situ or severe dysplasia. Most of them occurred in the oral cavity (77%). RESULTS: A higher incidence (18% vs 9%, p = .037) and less-advanced stage (4% vs 37%, p = .0005) of second primary malignancies were found among the NBI group compared with a previous cohort without NBI examination, and fewer patients needed postoperative adjuvant therapy (12% vs 50%, p = .0005). CONCLUSIONS: NBI is an effective method to identify early lesions in the head and neck area, especially the oral cavity, among patients with OSCC after treatment.


Subject(s)
Carcinoma, Squamous Cell/prevention & control , Carcinoma, Squamous Cell/surgery , Early Detection of Cancer/methods , Endoscopy/methods , Head and Neck Neoplasms/prevention & control , Head and Neck Neoplasms/surgery , Mouth Neoplasms/surgery , Neoplasms, Second Primary/diagnosis , Precancerous Conditions/diagnosis , Tongue Neoplasms/surgery , Adult , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/pathology , Cheek , Female , Head and Neck Neoplasms/pathology , Humans , Hypopharyngeal Neoplasms/diagnosis , Hypopharyngeal Neoplasms/prevention & control , Image Enhancement/methods , Male , Middle Aged , Mouth Mucosa/pathology , Neoplasm Staging , Oropharyngeal Neoplasms/diagnosis , Oropharyngeal Neoplasms/prevention & control , Squamous Cell Carcinoma of Head and Neck
4.
Asian Pac J Cancer Prev ; 12(2): 397-401, 2011.
Article in English | MEDLINE | ID: mdl-21545202

ABSTRACT

OBJECTIVE: To investigate the effects of caveolin-1 (CAV1) on the growth of hypopharyngeal squamous cell carcinoma (HSCC) FaDu cells in vitro and in vivo. METHODS: A CAV1-RNAi-lentivirus construct was transfected into FaDu cells and expression of caveolin-1 was tested by RT-PCR and western blotting analysis. Cell apoptosis was analyzed by transferase-medisated dUTP nick-end labeling (TUNEL) assay. Tumor inhibition effects were investigated by injecting rCAV1-RNAi-lentivirus construct into tumors created with FaDu cells in the HSCC mouse model, with the empty-vector lentivirus as a control. CAV1 expression in xenografts was tested by RT-PCR and immunohistochemistry. RESULTS: RT-PCR and western blot analysis demonstrated successful construction of the CAV1-RNAi-lentivirus construct producing small hairpin RNA. The average weights and volumes of tumor in mice treated with CAV1-RNAi-lentivirus were lower than in mice with control treatment (P < 0.05). RT-PCR revealed weak positive expression of CAV1 in CAV1-construct-treated xenografts and immunohistochemistry confirmed lower CAV1 expression than in controls.(P < 0.05). In addition, downregulation of CAV1 increased cell apoptosis in vitro. CONCLUSION: The growth of HSCCs could be inhibited by recombinant CAV1-RNAi-lentivirus in vitro and in vivo.


Subject(s)
Carcinoma, Squamous Cell/prevention & control , Caveolin 1/genetics , Genetic Vectors/administration & dosage , Hypopharyngeal Neoplasms/prevention & control , Lentivirus/genetics , RNA Interference , Animals , Apoptosis , Blotting, Western , Carcinoma, Squamous Cell/genetics , Carcinoma, Squamous Cell/pathology , Caveolin 1/antagonists & inhibitors , Caveolin 1/metabolism , Cell Proliferation , Humans , Hypopharyngeal Neoplasms/genetics , Hypopharyngeal Neoplasms/pathology , Immunoenzyme Techniques , In Vitro Techniques , Male , Mice , Mice, Inbred BALB C , Mice, Nude , RNA, Messenger/genetics , RNA, Small Interfering/genetics , Reverse Transcriptase Polymerase Chain Reaction , Tumor Cells, Cultured
5.
HNO ; 42(9): 530-40, 1994 Sep.
Article in German | MEDLINE | ID: mdl-7989224

ABSTRACT

We carried out a case-control study on the role of occupational factors on the risk of pharyngeal cancer. The study was performed at the Department of Otorhinolaryngology/Head and Neck Surgery of the University of Heidelberg and comprised 105 male patients with squamous cell carcinoma of the oropharynx and/or hypopharynx and 420 randomly selected control subjects who were matched for age, sex and residential area (1:4 matching design). The study showed that 34.3% of the cancer patients and 10.1% of the control subjects worked in the construction industry. As construction workers were 26.7% of the cancer patients and 7.1% of the control subjects employed, the relative risk of pharyngeal cancer in construction workers was estimated to be 2.5 (C.I. 1.1-5.5, adjusted for alcohol and tobacco consumption). After further adjustment for alcohol and tobacco consumption, an increased risk of pharyngeal cancer was found for workers exposed to cutting oils (RR = 3.7; C.I. 1.2-11.8), iron dust (RR = 2.7; C.I. 1.0-7.0) asbestos cement (RR = 2.5; C.I. 1.0-6.1), cement (RR = 2.2; C.I. 0.9-5.2) and coal/tar products (RR = 3.6; C.I. 0.8-17.3).


Subject(s)
Hypopharyngeal Neoplasms/etiology , Occupational Diseases/etiology , Occupational Exposure/adverse effects , Oropharyngeal Neoplasms/etiology , Adult , Aged , Alcohol Drinking/adverse effects , Alcohol Drinking/epidemiology , Case-Control Studies , Cross-Sectional Studies , Dust/adverse effects , Germany/epidemiology , Humans , Hypopharyngeal Neoplasms/epidemiology , Hypopharyngeal Neoplasms/prevention & control , Incidence , Male , Middle Aged , Occupational Diseases/epidemiology , Occupational Diseases/prevention & control , Occupations/statistics & numerical data , Oropharyngeal Neoplasms/epidemiology , Oropharyngeal Neoplasms/prevention & control , Risk Factors , Smoking/adverse effects , Smoking/epidemiology
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