ABSTRACT
It was set by us, that at intensifying of herpetic stomatititis of mucous membrane of oral cavity for patients the deficit of lysozyme and antibodies of class is marked A in the mixed saliva. At what the degree of expressed of the exposed violations of local immunity correlated with frequency of relapses of viral infection and its duration.
Subject(s)
IgA Deficiency/pathology , Immunity, Mucosal , Mouth Mucosa/immunology , Muramidase/deficiency , Stomatitis, Herpetic/immunology , Adult , Female , Humans , IgA Deficiency/enzymology , IgA Deficiency/virology , Immunoglobulin A/metabolism , Male , Middle Aged , Mouth Mucosa/enzymology , Mouth Mucosa/pathology , Mouth Mucosa/virology , Recurrence , Saliva/chemistry , Severity of Illness Index , Simplexvirus , Stomatitis, Herpetic/enzymology , Stomatitis, Herpetic/pathology , Stomatitis, Herpetic/virologyABSTRACT
OBJECTIVES: We aimed to investigate the activity of ADA and its isoenzymes in serum of patients with various primary immunodeficiency (PID) syndromes. DESIGN AND METHODS: Total ADA (tADA) and its isoenzymes were measured in 76 children with PID syndromes and 30 healthy controls using the Ellis method. RESULTS: Our results indicated that tADA and ADA2 levels were higher in patients with Chronic Granulomatous Disease (CGD), Leukocyte Adhesion Deficiency (LAD), hyper IgM (HIM) and Wiskott-Aldrich Syndrome (WAS) than those of corresponding controls (P<0.01). There was a significant elevation of tADA and ADA1 activities in IgA deficiency patients as compared to healthy individuals (P<0.01). CONCLUSIONS: Our results hypothesized that altered ADA activity may be associated with altered immunity. Therefore, serum ADA level could be used as an indicator along with other parameters in follow up of patients with CGD, LAD, IgA deficiency, HIM and WAS.
Subject(s)
Adenosine Deaminase/blood , Severe Combined Immunodeficiency/enzymology , Adenosine Deaminase/metabolism , Adolescent , Analysis of Variance , Child , Child, Preschool , Granulomatous Disease, Chronic/enzymology , Humans , Hyper-IgM Immunodeficiency Syndrome/enzymology , IgA Deficiency/enzymology , IgG Deficiency/enzymology , Infant , Iran , Isoenzymes/blood , Isoenzymes/metabolism , Leukocyte-Adhesion Deficiency Syndrome/enzymology , Severe Combined Immunodeficiency/pathologyABSTRACT
HLA-B14 positive haplotypes have increased frequencies in a group of patients with puberty disorders, IgA deficiency and cancer of the ovary. Clinical investigations demonstrated that all these patients have high values of 170H progesteron after the ACTH test which suggests an alterated function of 21 hydroxylase enzyme. In order to investigate whether these B14 positive haplotypes carry the same CYP21 mutation in the various diseases and controls, we have amplified by polymerase chain reaction (PCR) the sections of CYP21B gene which include amino acid positions 172 and 281 where typical mutations are known to occur in 21 hydroxylase deficiency. The presence or absence of the defined mutations was tested by oligonucleotide hybridization using oligonucleotides, labelled with DIG-ddUTP, designed to hybridize with the mutated or with the normal sequence. It was found that regardless of whether the subject tested was a patient or a healthy control the mutation at position 281 was found in all cases carrying HLA-B14, DR1 haplotype. Interestingly, this mutation does not seem to be in association with HLA-B14, DR7 haplotype. These findings suggest that CYP21 gene plays a role in all these differing diseases although it must be stressed that there may be alternative explanations for the observed data.
