Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Publication year range
1.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 34(3): 205-210, 2018 Mar.
Article in Chinese | MEDLINE | ID: mdl-29773100

ABSTRACT

Objective To construct eukaryotic expression vectors of human IgE heavy chain 2-4 region (IgECepsilon2-4) and purify the recombinant protein, and then capture its interacted proteins by surface plasmon resonance (SPR). Methods Three recombinant eukaryotic expression vectors of IgECepsilon2-4 containing different signal peptides were constructed and transiently transfected into HEK293FT suspension cells separately. The recombinant plasmid with the highest-level expression was selected to express the recombinant protein in a huge amount, and then the recombinant protein was purified by Ni-NTA affinity chromatography. The interaction between high-affinity IgE receptor (FcepsilonR I) of KU812 cell surface and IgECepsilon2-4 was identified by immunofluorescence cytochemistry. The unknown proteins that specifically interacted with IgECepsilon2-4 were captured from human serum by SPR technique. Results The recombinant plasmid containing the signal peptide III showed the highest expression (6.2 mg/L). Highly purified recombinant protein IgECepsilon2-4 was obtained by affinity purification. Immunofluorescence cytochemistry showed that the recombinant protein IgECepsilon2-4 could be combined with the surface receptor of KU812 cells. Thirty-nine kinds of proteins which were likely to interact with IgECepsilon2-4 were captured from human serum by SPR. Conclusion We obtained the purified recombinant protein IgECepsilon2-4 that could be combined with KU812 cell surface receptor. Target fishing experiment revealed that the recombinant protein IgECepsilon2-4 was likely to interact with 39 kinds of proteins in human serum.


Subject(s)
Eukaryotic Cells/metabolism , Immunoglobulin E/genetics , Immunoglobulin epsilon-Chains/genetics , Immunoglobulin epsilon-Chains/isolation & purification , Blotting, Western , Chromatography, Affinity , Cloning, Molecular , HEK293 Cells , Humans , Immunoglobulin E/analysis , Immunoglobulin E/isolation & purification , Immunoglobulin E/metabolism , Immunoglobulin epsilon-Chains/analysis , Immunoglobulin epsilon-Chains/metabolism , Recombinant Proteins/analysis , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Transfection
2.
Vet Immunol Immunopathol ; 59(3-4): 253-70, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9477476

ABSTRACT

An equine immunoglobulin E (IgE) heavy-chain cDNA fragment (CH3-CH4, nucleotides 1132 to 1592) was cloned, expressed in Escherichia coli as a fusion protein with a [His]6-tag and purified over a Ni-NTA column. The recombinant protein was used to immunise hens. Testing of the raised egg yolk immunoglobulin G (IgG) in Western-blot and ELISA revealed high titres against the recombinant equine IgE fragment (reqIgEf). The reqIgEf-specific IgG was successfully affinity-purified on an unconventional affinity matrix: the [His]6-tagged recombinant IgE fragment was bound to Ni-NTA agarose and used to adsorb specific immunoglobulins. In Western-blot of ammonium sulphate precipitated horse serum and bronchoalveolar lavage fluid, separated by SDS-PAGE under denaturing-reducing conditions, the raised antibodies reacted with a protein of approximately 80 kDa. A reaction of the reqIgEf-specific IgG was seen with a 190-200 kDa band when the same horse serum or bronchoalveolar fluid (BALF) was separated under non-reducing conditions. These reactions could be inhibited by preincubation of the immune IgG with reqIgEf, while preincubation with horse IgG did not inhibit the reaction. Antibody-affinity chromatography of horse serum with the reqIgEf-specific chicken IgG resulted in an enrichment of the 80 kDa protein in denaturing Western-blot. Determination of the amino acid composition of this protein and comparison with the equine IgE heavy- chain sequence strongly indicates that the 80 kDa band corresponds to the heavy chain of the horse IgE. The reqIgEf-specific chicken IgG was further characterised in an ELISA for the detection of allergen-specific horse IgE. It was demonstrated that heating IgE positive horse sera at 54 degrees C for 10 min drastically diminished the recognition by the reqIgEf-specific chicken IgG. The reaction is inhibitable by preincubation with reqIgEf in a concentration dependent manner. In addition, preincubation with horse IgG, a nonrelevant [His]6-tagged protein or 2% equine colostrum had no influence on the reqIgEf-specific chicken IgG binding characteristic. This antibody recognising horse IgE will be useful for further studies on the pathogenesis of equine allergic diseases.


Subject(s)
Antibodies, Anti-Idiotypic/immunology , Immunoglobulin E/immunology , Immunoglobulin epsilon-Chains/immunology , Amino Acids/analysis , Anaphylaxis/immunology , Anaphylaxis/veterinary , Animals , Chickens , Chromatography, Affinity , Cloning, Molecular , Colostrum/immunology , Escherichia coli , Goats , Horses , Hot Temperature , Immunoblotting , Immunoglobulin epsilon-Chains/genetics , Immunoglobulin epsilon-Chains/isolation & purification , Indicators and Reagents , Rabbits , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunology , Recombinant Fusion Proteins/isolation & purification
3.
FEBS Lett ; 224(2): 306-10, 1987 Nov 30.
Article in English | MEDLINE | ID: mdl-3121387

ABSTRACT

Complementary DNA of human IgE Fc epsilon fragment (residues 226-480) lacking the C epsilon 4 domain was expressed in Escherichia coli and the product was purified by immunoaffinity chromatography on a monoclonal antibody (E12 0.02)-Affi-Gel 10 column. About 1.8 mg of an apparent dimer and 5.9 mg of a monomer were obtained from 65 g E. coli cells with 9.3% recovery. The purified products were found to lack more than half of the COOH-terminal portion of the C epsilon 3 domain. The apparent dimer showed high immunological specific activity (3.6 x 10(6) U/mg protein) comparable to that of natural human IgE when measured by commercial human IgE determination kits.


Subject(s)
Immunoglobulin Fc Fragments/isolation & purification , Immunoglobulin Heavy Chains/isolation & purification , Immunoglobulin epsilon-Chains/isolation & purification , Recombinant Proteins/isolation & purification , Chromatography , DNA/genetics , Escherichia coli , Humans , Immunoglobulin Fc Fragments/genetics , Immunoglobulin epsilon-Chains/genetics , Molecular Weight
4.
Eur J Immunol ; 15(9): 966-9, 1985 Sep.
Article in English | MEDLINE | ID: mdl-2412840

ABSTRACT

An immunoglobulin epsilon-chain fragment was synthesized in E. coli by cloning and expression of the gene coding for the second, third and fourth constant domains of the human IgE heavy chain. The bacterial CH2-4 polypeptide product was assembled by oxidation into a covalently linked dimeric epsilon-chain molecule presumably analogous to the Fc region of native IgE. This bacterial Fc epsilon preparation, within the concentration range 0.01-10 micrograms/ml, inhibited sensitization of human lung mast cells, determined as histamine released upon challenge with specific antigen. Monomer CH2-4 epsilon-chain polypeptide, prepared by reduction and alkylation of the active bacterial Fc epsilon fragment, was inactive as an inhibitor of sensitization. The molar potency of the active bacterial Fc epsilon product was approximately one fourth of that of native IgE. Since the bacterial Fc epsilon is nonglycosylated, carbohydrate does not make an essential contribution to the Fc receptor binding activity of IgE. These results show that a functionally active immunoglobulin molecule can be synthesized by gene cloning and expression in E. coli.


Subject(s)
Escherichia coli/metabolism , Immunoglobulin Heavy Chains/physiology , Immunoglobulin epsilon-Chains/physiology , Immunosuppressive Agents , Mast Cells/immunology , Antibodies, Bacterial/biosynthesis , Antibodies, Bacterial/isolation & purification , Antibodies, Bacterial/physiology , Escherichia coli/immunology , Histamine Release , Humans , Immunoglobulin Fc Fragments/biosynthesis , Immunoglobulin Fc Fragments/isolation & purification , Immunoglobulin Fc Fragments/physiology , Immunoglobulin epsilon-Chains/biosynthesis , Immunoglobulin epsilon-Chains/isolation & purification , Lung/cytology
SELECTION OF CITATIONS
SEARCH DETAIL
...