ABSTRACT
The contamination of the cultivator with the extraneous microflora in the process of growing microorganisms in various apparatus has been shown to have the probabilistic character. The index of aseptic effectiveness is recommended for the comparative evaluation of the effectiveness of the processes which ensure the achievement and maintenance of aseptic conditions in the process of the cultivation of microorganisms in the laboratory and industrial equipment, as well as for changing the scale of the process.
Subject(s)
Antisepsis/methods , Asepsis/methods , Incubators/instrumentation , Microbiological Techniques/methods , Sterilization/methods , Vaccines , Bacteria/growth & development , Technology, Pharmaceutical , Viruses/growth & developmentABSTRACT
Primary, diploid and continuous cell lines were cultivated in the apparatus "Cellotron-1". Rashig's ring was used as an underlay in the apparatus. Fresh medium perfusion, pH control, the medium aeration, temperature control, as well as dissolved oxygen control were exercised in the cultivator. Various cell types have demonstrated a significant increase as compared to the control stationary cultivation: diploid green monkey embryo cells increased 7.5-fold for 5 days, continuous green monkey kidney cells 8-fold for 4 days, primary monkey kidney cells 2-fold for 7 days.
Subject(s)
Cytological Techniques/instrumentation , Incubators/instrumentation , Animals , Cell Adhesion , Cell Line , Cells, Cultured , Chlorocebus aethiops/embryology , Diploidy , KidneySubject(s)
Acid-Base Equilibrium , Incubators/instrumentation , Carbon Dioxide , Culture Media , Culture TechniquesSubject(s)
Breeding , Incubators , Poultry , Animals , Chickens , Egypt , Europe , History, 15th Century , History, 17th Century , History, 18th Century , History, Ancient , History, Medieval , Incubators/instrumentationABSTRACT
The use and construction of an automatic gas tank switching system are described. This device monitors the gas pressure in a CO2 incubator gas system and automatically switches to a reserve tank when the main supply tank is depleted. The unit contains an alarm system that signals either loss of power or gas pressure in the supply system.
Subject(s)
Culture Techniques/instrumentation , Incubators/instrumentation , Carbon Dioxide , PressureABSTRACT
An experimental chick hatcher designed to filter recycled ventilation air was tested for its effectiveness in reducing the number of viable airborne microorganisms. Chicks in a filtered hatcher and a control hatcher (no filter) were artificially contaminated with Serratia marcescens as ventilation air was recycled in the hatchers for twelve hours. The number of viable S. marcescens particles in the filtered air of the conditioning chamber was less than detectable. The number of viable airborne S. marcescens particles in the hatching chamber of the filtered hatcher indicated a reduction of greater than 90 percent over the number in the unfiltered hatcher. The filter was effective in reducing the number of airborne particles carrying viable S. marcescens organisms in the hatcher.
Subject(s)
Air Microbiology , Chickens , Incubators , Ventilation , Air , Animals , Chick Embryo , Filtration/instrumentation , Incubators/instrumentation , Serratia marcescens/isolation & purificationABSTRACT
Two experimental chick hatchers in which ventilation air within the hatcher was partially recirculated in a positive pressure system, one with an air filter in the system and the other without a filter, were simultaneously tested to determine effect of the filter on quantitative reduction of viable airborne microorganisms. Chicks were artificially contaminated with either Escherichia coli or Staphylococcus aureus (coagulase-positive). Air was sampled for total test bacteria per cubic foot of hatcher air. The filter effectively reduced the number of viable airborne particles contaminated with E. coli and S. aureus contributed by chicks in the hatcher.
Subject(s)
Air Microbiology , Bacteria/isolation & purification , Chickens , Incubators , Ventilation , Animals , Escherichia coli/isolation & purification , Escherichia coli/pathogenicity , Filtration , Incubators/instrumentation , Staphylococcus/isolation & purification , Staphylococcus/pathogenicityABSTRACT
It is reported on the evidence of phenoloxidase containing cells (=POZ) under various experimental conditions in as well spleen and small gut of white rats as small gut and skin of rabbit. A modified technic is described which gives a shortened incubation time and a regulary pigmentation of the reactive cells. POZ are demonstrable between pH = 6.2 and 8.0.
