ABSTRACT
In this study, we designed and developed a novel low-cost system for anesthetizing and injecting adult zebrafish. The system utilizes a gradual cooling method for the anesthesia and maintains the fish in a stable anesthetic plane, as well as stabilizes the animal so that intraperitoneal injections can be consistently performed. It is a system that any laboratory with access to a workshop can build for their group. Moreover, it is a safe system for researchers, as well as a reliable one for repeated experiments since multiple fish can be injected quickly and there is little physical contact necessary between the investigator and the animal. This will likely reduce any unnecessary stress in the fish, as compared with manual methods of injection. Finally, the system is adaptable so that as the investigators' procedural needs change due to different research questions, that is, gradual rewarming or something of that nature, it could be modified.
Subject(s)
Anesthesia/veterinary , Injections, Intraperitoneal/veterinary , Anesthesia/economics , Anesthesia/methods , Animals , Injections, Intraperitoneal/economics , Injections, Intraperitoneal/instrumentation , Injections, Intraperitoneal/methods , ZebrafishABSTRACT
Intraperitoneal (IP) injections are an effective and reproducible route of drug administration. However, current IP equipment can be either costly, inaccurate, or unsafe for zebrafish. We describe a simple, low-cost IP setup, which can be easily assembled from inexpensive and readily available parts, and which provides a safe, reproducible, and accurate IP-injection method for experimenters.
Subject(s)
Behavior, Animal , Injections, Intraperitoneal/veterinary , Zebrafish/growth & development , Animals , Equipment Design , Injections, Intraperitoneal/economics , Injections, Intraperitoneal/instrumentation , Injections, Intraperitoneal/methodsABSTRACT
Intradialytic parenteral nutrition and intraperitoneal nutrition are methods to provide supplemental nutrition support to patients with chronic kidney disease (also referred to as end-stage renal disease) while they are receiving dialytic therapy. There has been considerable controversy over indications and benefits of intradialytic parenteral nutrition and intraperitoneal nutrition. A major stumbling block had been problematic reimbursement for the therapy under Medicare. Medicare Part D has alleviated some of the reimbursement obstacles and has increased interest in reexamining the benefits and burdens of supplemental nutrition support therapies for patients receiving dialysis.
