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1.
Biomolecules ; 11(2)2021 02 07.
Article in English | MEDLINE | ID: mdl-33562198

ABSTRACT

This article aimed to identify and distinguish the various responses to silver nanoparticles (NPs) of endothelial and epithelial cells. We also assessed the significantly increased gene expression levels, as shown by microarray analysis. We evaluated the median lethal dose of NPs in each cell line and found that each value was different. We also confirmed the toxicity of 5 nm silver NPs. Meanwhile, cell death was not observed in cells exposed to 100 nm silver NPs at a high concentration. We verified that 5 nm silver NPs affected the variation in gene expression in cells through microarray analysis and observed a noticeable increase in interleukin (IL)-8 and IL-11 gene expression in early stages. This study showed noticeable variation in the expression of oxidative stress-related genes in early stages. Microarray results showed considerable variation in cell death-, apoptosis-, and cell survival-related gene expression. Of note, IL-11 gene expression was particularly increased following the exposure of endothelial and epithelial cells to 5 nm silver NPs. In conclusion, this study demonstrated that intracellular genes specifically responded to silver NPs in respiratory epithelial cells and endothelial cells. Among cytokine genes, IL-11 expression was noticeably increased. Additionally, we confirmed that NP toxicity was affected by NP size and dose.


Subject(s)
Bronchi/drug effects , Endothelial Cells/drug effects , Endothelium, Vascular/metabolism , Epithelial Cells/drug effects , Interleukin-11/biosynthesis , Metal Nanoparticles/chemistry , Silver/chemistry , Stress, Physiological , Apoptosis/drug effects , Cell Line , Cell Proliferation , Cell Survival/drug effects , Cytokines/metabolism , Gene Expression Profiling , Gene Expression Regulation/drug effects , Humans , Inflammation , Nanoparticles/chemistry , Oligonucleotide Array Sequence Analysis , Oxidative Stress , Umbilical Veins/drug effects
2.
Ocul Immunol Inflamm ; 28(4): 575-588, 2020 May 18.
Article in English | MEDLINE | ID: mdl-31403827

ABSTRACT

PURPOSE: To investigate the expression of IL-11 and its receptor IL-11Rα and to quantify density of CD163+ M2 macrophages in proliferative diabetic retinopathy (PDR). METHODS: Vitreous samples from 29 PDR and 19 nondiabetic patients, epiretinal fibrovascular membranes from 15 patients with PDR and Müller cells were studied by enzyme-linked immunosorbent assay, immunohistochemistry and Western blot analysis. RESULTS: We showed a significant increase in expression of IL-11, soluble(s) IL-11Rα, sCD163 and VEGF in vitreous samples from PDR patients compared to nondiabetic controls. Significant positive correlations were found between levels of VEGF and levels of IL-11 and sCD163. Significant positive correlations were found between microvessel density and number of blood vessels and stromal cells expressing IL-11, IL-11Rα and CD163 in PDR epiretinal membranes. The hypoxia mimetic agent cobalt chloride induced upregulation of IL-11 and IL-11Ra in cultured Müller cells. CONCLUSIONS: IL-11/IL-11Rα signaling and CD163+ M2 macrophages might be involved in PDR angiogenesis.


Subject(s)
Diabetic Retinopathy/genetics , Ependymoglial Cells/pathology , Gene Expression Regulation , Interleukin-11/genetics , Adult , Blotting, Western , Cell Count , Cells, Cultured , Diabetic Retinopathy/diagnosis , Diabetic Retinopathy/metabolism , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunohistochemistry , Interleukin-11/biosynthesis , Male , Middle Aged , RNA/genetics , Up-Regulation
3.
Front Immunol ; 10: 2312, 2019.
Article in English | MEDLINE | ID: mdl-31636637

ABSTRACT

To date, available treatment strategies for multiple sclerosis (MS) are ineffective in preventing or reversing progressive neurologic deterioration, creating a high, and unmet medical need. One potential way to fight MS may be by limiting the detrimental effects of reactive astrocytes, a key pathological hallmark for disease progression. One class of compounds that may exert beneficial effects via astrocytes are melanocortin receptor (MCR) agonists. Among the MCR, MC4R is most abundantly expressed in the CNS and several rodent studies have described that MC4R is-besides neurons-expressed by astrocytes. Activation of MC4R in astrocytes has shown to have potent anti-inflammatory as well as neuroprotective effects in vitro, suggesting that this could be a potential target to ameliorate ongoing inflammation, and neurodegeneration in MS. In this study, we set out to investigate human MC4R expression and analyze its downstream effects. We identified MC4R mRNA and protein to be expressed on astrocytes and observed increased astrocytic MC4R expression in active MS lesions. Furthermore, we show that the novel, highly selective MC4R agonist setmelanotide ameliorates the reactive phenotype in astrocytes in vitro and markedly induced interleukin-6 and -11 production, possibly through enhanced cAMP response element-binding protein (CREB) phosphorylation. Notably, stimulation of human macrophages with medium from astrocytes that were exposed to setmelanotide, skewed macrophages toward an anti-inflammatory phenotype. Taken together, these findings suggest that targeting MC4R on astrocytes might be a novel therapeutic strategy to halt inflammation-associated neurodegeneration in MS.


Subject(s)
Anti-Inflammatory Agents/pharmacology , Astrocytes/drug effects , Macrophages/drug effects , Receptor, Melanocortin, Type 4/agonists , alpha-MSH/analogs & derivatives , Adult , Aged , Cells, Cultured , Cyclic AMP Response Element-Binding Protein/metabolism , Female , Humans , Interleukin-11/biosynthesis , Interleukin-6/biosynthesis , Male , Middle Aged , Multiple Sclerosis/drug therapy , Phenotype , Phosphorylation , Receptor, Melanocortin, Type 4/drug effects , Receptor, Melanocortin, Type 4/genetics , alpha-MSH/pharmacology
4.
Cell Death Dis ; 10(1): 2, 2018 12 18.
Article in English | MEDLINE | ID: mdl-30578411

ABSTRACT

CUL1 is an essential component of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Our previous study has showed that CUL1 is positively associated with poor overall and disease-specific survival of breast cancer patients. Here, we further explored its roles in breast cancer metastasis. Our data showed that CUL1 significantly promoted breast cancer cell migration, invasion, tube formation in vitro, as well as angiogenesis and metastasis in vivo. In mechanism, the human gene expression profiling was used to determine global transcriptional changes in MDA-MB-231 cells, and we identified autocrine expression of the cytokines CXCL8 and IL11 as the target genes of CUL1 in breast cancer cell migration, invasion, metastasis, and angiogenesis. CUL1 regulated EZH2 expression to promote the production of cytokines, and finally significantly aggravating the breast cancer cell metastasis and angiogenesis through the PI3K-AKT-mTOR signaling pathway. Combined with the previous report about CUL1, we proposed that CUL1 may serve as a promising therapeutic target for breast cancer metastasis.


Subject(s)
Autocrine Communication , Breast Neoplasms/metabolism , Cullin Proteins/metabolism , Enhancer of Zeste Homolog 2 Protein/metabolism , Gene Expression Regulation, Neoplastic , Interleukin-11/biosynthesis , Interleukin-8/biosynthesis , Neoplasm Proteins/metabolism , Animals , Breast Neoplasms/genetics , Breast Neoplasms/pathology , Cullin Proteins/genetics , Enhancer of Zeste Homolog 2 Protein/genetics , Human Umbilical Vein Endothelial Cells , Humans , Interleukin-11/genetics , Interleukin-8/genetics , MCF-7 Cells , Mice , Mice, Inbred BALB C , Mice, Nude , Neoplasm Metastasis , Neoplasm Proteins/genetics
5.
Clin Chim Acta ; 481: 193-199, 2018 Jun.
Article in English | MEDLINE | ID: mdl-29555322

ABSTRACT

BACKGROUND: Interleukin (IL) 11 is closely related to tumor and hematological system diseases. Recent studies have demonstrated that IL-11 also participates in cardiovascular diseases, including ischemia-reperfusion mediated heart injury and acute myocardial infarction. This study aimed to investigate whether IL-11 is involved in acute thoracic aortic dissection (TAD). METHODS: Aortic tissue samples from normal donors and acute TAD patients were collected, and the expression of IL-11 in all aortic tissue was analyzed. In addition, blood samples from patients with chest pain were collected and divided into a non-AD (NAD) group and a TAD group according to the results of computed tomography angiography of the thoracic aorta. The plasma IL-11, IL-17 and interferon (IFN) γ in all blood samples were measured. RESULTS: Compared with aortic tissue of normal controls, IL-11 was significantly increased in aortic tissue of acute TAD patients, especially in the torn section. The IL-11 was derived from aorta macrophages in TAD. In addition, the plasma IL-11, IL-17 and IFN-γ were significantly higher in acute TAD patients than in NAD patients, and the correlation analysis showed that IL-11 levels were positively correlated with levels of IFN-γ, IL-17, glucose, systolic blood pressure, diastolic blood pressure, white blood cells, C-reactive proteins and D-dimers. Binary logistic regression analyses showed that elevated IL11 in patients who may have diagnostic value of TAD, but less that D-dimer. CONCLUSION: IL-11 was increased in thoracic aorta and plasma of TAD patients and may be a promising biomarker for diagnosis in patients with TAD.


Subject(s)
Aorta, Thoracic/chemistry , Aortic Aneurysm, Thoracic/blood , Aortic Dissection/blood , Aortic Dissection/diagnosis , Interleukin-11/blood , Adult , Aged , Aortic Aneurysm, Thoracic/diagnosis , Female , Humans , Interleukin-11/biosynthesis , Male , Middle Aged , Regression Analysis
7.
ACS Synth Biol ; 6(12): 2260-2272, 2017 12 15.
Article in English | MEDLINE | ID: mdl-29136368

ABSTRACT

Trans-signaling of the major pro- and anti-inflammatory cytokines Interleukin (IL)-6 and IL-11 has the unique feature to virtually activate all cells of the body and is critically involved in chronic inflammation and regeneration. Hyper-IL-6 and Hyper-IL-11 are single chain designer trans-signaling cytokines, in which the cytokine and soluble receptor units are trapped in one complex via a flexible peptide linker. Albeit, Hyper-cytokines are essential tools to study trans-signaling in vitro and in vivo, the superior potency of these designer cytokines are accompanied by undesirable stress responses. To enable tailor-made generation of Hyper-cytokines, we developed inactive split-cytokine-precursors adapted for posttranslational reassembly by split-intein mediated protein trans-splicing (PTS). We identified cutting sites within IL-6 (E134/S135) and IL-11 (G116/S117) and obtained inactive split-Hyper-IL-6 and split-Hyper-IL-11 cytokine precursors. After fusion with split-inteins, PTS resulted in reconstitution of active Hyper-cytokines, which were efficiently secreted from transfected cells. Our strategy comprises the development of a background-free cytokine signaling system from reversibly inactivated precursor cytokines.


Subject(s)
Immunoglobulin Constant Regions , Interleukin-11 , Interleukin-6 , Recombinant Fusion Proteins , Trans-Splicing , Animals , COS Cells , Chlorocebus aethiops , HEK293 Cells , Humans , Immunoglobulin Constant Regions/biosynthesis , Immunoglobulin Constant Regions/genetics , Interleukin-11/biosynthesis , Interleukin-11/genetics , Interleukin-6/biosynthesis , Interleukin-6/genetics , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/genetics
8.
Oncogene ; 36(45): 6315-6324, 2017 11 09.
Article in English | MEDLINE | ID: mdl-28714957

ABSTRACT

The interaction between cancer cells and their microenvironment is an important determinant of the pathological nature of cancers, particularly their tumorigenic abilities. The KEAP1-NRF2 system, originally identified as a critical defense mechanism against oxidative stress, is often dysregulated in various human cancers forming solid tumors, resulting in the aberrant activation of NRF2. Increased accumulation of NRF2 in cancers is strongly associated with the poor prognoses of cancer patients, including those with lung and breast cancers. Multiple lines of evidence suggest that aberrantly activated NRF2 in cancer cells drives their malignant progression and that the cancer cells consequently develop 'NRF2 addiction.' Although the downstream effectors of NRF2 that are responsible for cancer malignancy have been extensively studied, mechanisms of how NRF2 activation contributes to the aggressive tumorigenesis remains to be elucidated. In this study, we found a significant correlation between NRF2 and IL-11 status in breast cancer patients. Based on a recent report demonstrating that IL-11 is induced downstream of NRF2, we examined the significance of IL-11 in NRF2-driven tumorigenesis with a newly established NRF2 addiction cancer model. Expression of Il11 was elevated during the tumorigenesis of the NRF2 addiction cancer model, but intriguingly, it was hardly detected when the cancer model cells were cultured in vitro. These results imply that a signal originating from the microenvironment cooperates with NRF2 to activate Il11. To the best of our knowledge, this is the first report showing the influence of the microenvironment on the NRF2 pathway in cancer cells and the contribution of NRF2 to the secretory phenotypes of cancers. Disruption of Il11 in the NRF2 addiction cancer model remarkably inhibited the tumorigenesis, suggesting an essential role of IL-11 in NRF2-driven tumorigenesis. Thus, this study suggests that IL-11 is a potential therapeutic target for NRF2-addicted breast cancers.


Subject(s)
Breast Neoplasms/pathology , Interleukin-11/biosynthesis , NF-E2-Related Factor 2/biosynthesis , Animals , Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Carcinogenesis , Cell Line, Tumor , Female , Heterografts , Humans , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Nude , Signal Transduction
9.
J Biol Chem ; 292(1): 205-216, 2017 Jan 06.
Article in English | MEDLINE | ID: mdl-27872193

ABSTRACT

Nuclear factor erythroid 2-related factor 2 (NRF2) is a transcription factor that plays a crucial role in protection of cells from electrophile-induced toxicity through up-regulating phase II detoxifying enzymes and phase III transporters. We previously reported that oxidative stress induces up-regulation of interleukin-11 (IL-11), a member of the IL-6 family that ameliorates acetaminophen-induced liver toxicity. However, a role for IL-11 in protection of cells from electrophile-induced toxicity remains unclear. Here we show that an environmental electrophile, 1,2-naphthoquinone (1,2-NQ), but not 15d-prostaglandin J2 (PGJ2) or tert-butylhydroxyquinone (tBHQ), induced IL-11 production. Consistent with a crucial role for prolonged ERK activation in H2O2-induced IL-11 production, 1,2-NQ, but not 15d-PGJ2 or tBHQ, elicited prolonged ERK activation. Conversely, inhibition of the ERK pathway by a MEK inhibitor completely blocked 1,2-NQ-induced IL-11 production at both protein and mRNA levels, further substantiating an intimate cross-talk between ERK activation and 1,2-NQ-induced IL-11 production. Promoter analysis of the Il11 gene revealed that two AP-1 sites were essential for 1,2-NQ-induced promoter activities. Among various members of the AP-1 family, Fra-1 was up-regulated by 1,2-NQ, and its up-regulation was blocked by a MEK inhibitor. Although NRF2 was not required for H2O2-induced IL11 up-regulation, NRF2 was essential for 1,2-NQ-induced IL11 up-regulation by increasing Fra-1 proteins possibly through promoting mRNA translation of FOSL1 Finally, intraperitoneal administration of 1,2-NQ induced body weight loss in wild-type mice, which was further exacerbated in Il11ra1-/- mice compared with Il11ra1+/- mice. Together, both Fra-1 and NRF2 play crucial roles in IL-11 production that protects cells from 1,2-NQ intestinal toxicity.


Subject(s)
Interleukin-11/biosynthesis , Intestinal Diseases/prevention & control , NF-E2-Related Factor 2/metabolism , Naphthoquinones/toxicity , Peritonitis/prevention & control , Prostaglandin D2/analogs & derivatives , Animals , Antineoplastic Agents/toxicity , Cells, Cultured , Gene Expression Regulation/drug effects , HEK293 Cells , Hep G2 Cells , Humans , Hydrogen Peroxide/pharmacology , Interleukin-11 Receptor alpha Subunit/physiology , Intestinal Diseases/chemically induced , Intestinal Diseases/metabolism , Intestinal Diseases/pathology , MAP Kinase Signaling System , Mice , Mice, Inbred C57BL , Mice, Knockout , NF-E2-Related Factor 2/genetics , Oxidants/pharmacology , Oxidative Stress/drug effects , Peritonitis/chemically induced , Peritonitis/metabolism , Peritonitis/pathology , Prostaglandin D2/toxicity , Reactive Oxygen Species/metabolism
10.
Genet Mol Res ; 15(2)2016 Apr 25.
Article in English | MEDLINE | ID: mdl-27173233

ABSTRACT

The expression of interleukin-11 (IL-11) and its products STAT3 and phospho-STAT3 (p-STAT3) in patients with chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG), and gastric cancer (GC) may provide insight into the diagnostic role of the IL-11/STAT3 signaling pathway in GC. Gastric mucosa specimens and serum samples were collected from 90 patients with CSG, CAG, and GC (30/group). The expression of IL-11, STAT3, and p-STAT3 was detected via immunohistochemistry and western blot. Additionally, serum levels of IL-11 were measured by enzyme-linked immunosorbent assay. For IL-11, 60% stained positive in CAG and 83.3% stained positive in GC, which were both higher than the value observed for CSG (33.3%). Moreover, the percent positive for IL-11 in GC was higher than that in CAG (P < 0.05). The percent positive for STAT3 in CAG (80%) and GC (83.3%) was higher than that in CSG (53.3%) (P < 0.05). Compared with CSG (36.7%), the percent positive for p-STAT3 in CAG (63.3%) and GC (86.7%) was also significantly higher. STAT3 expression was similar in GC and CAG, which was significantly higher than that in CSG. Expectedly, p-STAT3 expression gradually increased from CSG to CAG to GC. Furthermore, p-STAT3 levels were higher in GC tissues than in CAG (P < 0.01). Intriguingly, serum IL-11 levels gradually increased from CSG to CAG to GC, which coincided with disease severity. Together, these results suggest that the IL-11/STAT3 signaling pathway plays a critical role in human CAG, and may provide new targets to prevent and treat GC.


Subject(s)
Gastritis, Atrophic/genetics , Interleukin-11/biosynthesis , STAT3 Transcription Factor/biosynthesis , Stomach Neoplasms/genetics , Aged , Female , Gastric Mucosa/pathology , Gastritis, Atrophic/pathology , Humans , Interleukin-11/genetics , Male , Middle Aged , Precancerous Conditions/genetics , Precancerous Conditions/pathology , STAT3 Transcription Factor/genetics , Signal Transduction , Stomach Neoplasms/pathology
11.
Arch Oral Biol ; 69: 25-32, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27232357

ABSTRACT

OBJECTIVE: This study aimed to evaluate the prognostic implications of insulin-like growth factor-II mRNA binding protein-3 (IMP3) and podoplanin (PDPN) as therapeutic targets against oral squamous cell carcinoma (OSCC) with bone invasion. STUDY DESIGN: We elucidated the correlation of IMP3 and PDPN expression with bone invasion in 160 OSCC tissue specimens, and assessed a mouse calvarium xenograft model using an IMP3- and PDPN-depleted OSCC cell line. RESULTS: The retrospective analysis revealed that the expression of IMP3 and PDPN is significantly correlated with T stage, lymph node metastasis, and the overall survival of OSCC patients. In addition, the dual expression of IMP3 and PDPN but not the single expression of either IMP3 or PDPN was associated with bone invasion and the number of osteoclasts in patients with OSCC. In support of these findings, IMP3 or PDPN depletion inhibited the invasive capacity of OSCC cells in a three-dimensional culture system, tumorigenesis, and regional bone destruction in a xenograft mouse model. In addition, IMP3 or PDPN depletion inhibited the expression of interleukin (IL)-6 and IL-8 in OSCC cells, and decreased the expression of receptor activator of NF-κB ligand (RANKL) in xenograft tumor tissues of OSCC. CONCLUSIONS: These results suggest that IMP3 and PDPN may have strong influence on the pathogenesis of OSCC, especially in bone invasion, and may serve as novel therapeutic targets with prognostic implications for bone-invasive OSCC.


Subject(s)
Bone Neoplasms/metabolism , Bone Neoplasms/secondary , Carcinoma, Squamous Cell/metabolism , Head and Neck Neoplasms/metabolism , Membrane Glycoproteins/pharmacology , Mouth Neoplasms/metabolism , RNA-Binding Proteins/pharmacology , Adult , Aged , Aged, 80 and over , Animals , Carcinoma, Squamous Cell/pathology , Cell Transformation, Neoplastic , Female , Head and Neck Neoplasms/pathology , Heterografts , Humans , Interleukin-11/biosynthesis , Interleukin-6/biosynthesis , Interleukin-8/biosynthesis , Lymphatic Metastasis , Male , Membrane Glycoproteins/biosynthesis , Membrane Glycoproteins/genetics , Mice , Middle Aged , Mouth Neoplasms/pathology , Osteoclasts/pathology , Prognosis , RANK Ligand/biosynthesis , RANK Ligand/drug effects , RNA, Messenger/genetics , RNA-Binding Proteins/biosynthesis , RNA-Binding Proteins/genetics , Receptor Activator of Nuclear Factor-kappa B/biosynthesis , Receptor Activator of Nuclear Factor-kappa B/drug effects , Retrospective Studies , Squamous Cell Carcinoma of Head and Neck
12.
Oncotarget ; 7(18): 26247-58, 2016 May 03.
Article in English | MEDLINE | ID: mdl-27028859

ABSTRACT

Mechanisms of stromal-epithelial crosstalk are essential for Prostate cancer (PCa) tumorigenesis and progression. Peripheral zone of the prostate gland possesses a stronger inclination for PCa than transition zone. We previously found a variety of genes that differently expressed among different prostate stromal cells, including LIM domain only 2 (LMO2) which highly expressed in peripheral zone derived stromal cells (PZSCs) and PCa associated fibroblasts (CAFs) compared to transition zone derived stromal cells (TZSCs). Studies on its role in tumors have highlighted LMO2 as an oncogene. Herein, we aim to study the potential mechanisms of stromal LMO2 in promoting PCa progression. The in vitro cells co-culture and in vivo cells recombination revealed that LMO2 over-expressed prostate stromal cells could promote the proliferation and invasiveness of either prostate epithelial or cancer cells. Further protein array screening confirmed that stromal LMO2 stimulated the secretion of Interleukin-11 (IL-11), which could promote proliferation and invasiveness of PCa cells via IL-11 receptor α (IL11Rα) - STAT3 signaling. Moreover, stromal LMO2 over-expression could suppress miR-204-5p which was proven to be a negative regulator of IL-11 expression. Taken together, results of our study demonstrate that prostate stromal LMO2 is capable of stimulating IL-11 secretion and by which activates IL11Rα - STAT3 signaling in PCa cells and then facilitates PCa progression. These results may make stromal LMO2 responsible for zonal characteristic of PCa and as a target for PCa microenvironment-targeted therapy.


Subject(s)
Adaptor Proteins, Signal Transducing/biosynthesis , Interleukin-11/biosynthesis , LIM Domain Proteins/biosynthesis , Prostatic Neoplasms/pathology , Proto-Oncogene Proteins/biosynthesis , Stromal Cells/metabolism , Animals , Disease Progression , Heterografts , Humans , Male , Mice , Paracrine Communication/physiology , Prostatic Neoplasms/metabolism , Stromal Cells/pathology , Up-Regulation
13.
Int J Paediatr Dent ; 26(5): 351-6, 2016 Sep.
Article in English | MEDLINE | ID: mdl-26440733

ABSTRACT

BACKGROUND: Coeliac disease is a chronic small intestinal immune-mediated enteropathy precipitated by exposure to dietary gluten, and possible relationships between coeliac disease and dental pathogenic conditions during childhood have been poorly investigated. AIM: The dental pulp plays a pivotal role in the immune defence against possible entry of pathogens from teeth, and the aim of this work was to investigate quantitative transcription levels of selected genes (IL-9, IL-11, IL-15, IL-18, IL-21, IL-27, MICA, IFN-γ) coding for pro-inflammatory immune innate activities in the pulp of primary teeth from healthy children and children with coeliac disease. DESIGN: The pulp from primary teeth of 10 healthy children and 10 children with coeliac disease was used to extract RNA and prepare cDNA for quantitative PCR transcription analysis employing commercial nucleotide probes for selected genes. RESULTS: In children with coeliac disease, the genes coding for pro-inflammatory cytokines IFN-γ, IL-11, IL-18, and IL-21 were significantly overexpressed, suggesting the possible importance of these cytokines in the relationships between coeliac disease and dental disorders. CONCLUSION: For the first time, we reported in dental pulp of children possible relationships between coeliac disease and modulation in transcription of cytokine-dependent inflammatory activities.


Subject(s)
Celiac Disease/complications , Cytokines/biosynthesis , Cytokines/genetics , Dental Pulp/immunology , Dental Pulp/metabolism , Inflammation/genetics , Inflammation/immunology , Child , Child, Preschool , Female , Gene Expression Regulation , Histocompatibility Antigens Class I/biosynthesis , Histocompatibility Antigens Class I/genetics , Humans , Immunity, Innate , Interferon-gamma/biosynthesis , Interferon-gamma/genetics , Interleukin-11/biosynthesis , Interleukin-11/genetics , Interleukin-15/biosynthesis , Interleukin-15/genetics , Interleukin-18/biosynthesis , Interleukin-18/genetics , Interleukin-9/biosynthesis , Interleukin-9/genetics , Interleukins/biosynthesis , Interleukins/genetics , Male , RNA/analysis , Real-Time Polymerase Chain Reaction , Tooth, Deciduous/immunology , Tooth, Deciduous/metabolism
14.
Am J Reprod Immunol ; 73(6): 545-56, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25648617

ABSTRACT

PROBLEM: We investigated the effect of Xianziyizhen recipe capsule (XRC), a kidney-tonifying herb, on the PGI2-PPARδ signaling pathway at the maternal-fetal interface in embryo implantation dysfunction (EID) mice. METHOD OF STUDY: Intragastric administration of Progynova (estradiol) or XRC was performed in EID mouse model, following experimental induction of kidney deficiency by co-treatment with chemotherapy drug hydroxyurea and antiprogesterone mifepristone. The PPARδ and IL-11 mRNA expression in endometrium were detected by real-time relative reverse transcription-polymerase chain reaction (RT-PCR). Further, the protein expression of COX-2, PGI2, MMP-9, and TIMP-3 was detected in endometrial glandular epithelium and in stromal cells by immunohistochemical (IHC) assay. RESULTS: The results showed that hydroxyurea and mifepristone-induced EID were associated with significantly lower PPARδ and IL-11 mRNA levels in endometrium and reduced COX-2, PGI2, MMP-9, and TIMP-3 levels in endometrial glandular epithelium, compared with normal controls. However, XRC and Progynova treatment reversed these effects, leading to significant increases in PPARδ and IL-11 mRNA expression, and COX-2, PGI2, MMP-9 and TIMP-3 protein levels, when compared with the levels observed in EID mice. CONCLUSION: These results strongly suggested that XRC is beneficial in EID treatment and that XRC may mediate its effects through regulation of the PGI2-PPARδ signaling pathway.


Subject(s)
Drugs, Chinese Herbal/pharmacology , Embryo Implantation/drug effects , Endometrium/immunology , Epoprostenol/immunology , Receptors, Cytoplasmic and Nuclear/immunology , Animals , Cyclooxygenase 2/biosynthesis , Cyclooxygenase 2/immunology , Embryo Implantation/immunology , Endometrium/cytology , Epithelial Cells/cytology , Epithelial Cells/immunology , Epoprostenol/biosynthesis , Female , Interleukin-11/biosynthesis , Interleukin-11/immunology , Matrix Metalloproteinase 9/biosynthesis , Matrix Metalloproteinase 9/immunology , Mice , Pregnancy , Receptors, Cytoplasmic and Nuclear/metabolism , Stromal Cells/cytology , Stromal Cells/immunology , Tissue Inhibitor of Metalloproteinase-3/biosynthesis , Tissue Inhibitor of Metalloproteinase-3/immunology
15.
Anesthesiology ; 119(6): 1389-401, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24037316

ABSTRACT

BACKGROUND: Isoflurane releases renal tubular transforming growth factor-ß1 (TGF-ß1) and protects against ischemic acute kidney injury. Recent studies suggest that TGF-ß1 can induce a cytoprotective cytokine interleukin (IL)-11. In this study, the authors tested the hypothesis that isoflurane protects against ischemic acute kidney injury by direct induction of renal tubular IL-11 synthesis. METHODS: Human kidney proximal tubule cells were treated with 1.25-2.5% isoflurane or carrier gas (room air + 5% carbon dioxide) for 0-16 h. The authors also anesthetized C57BL/6 mice with 1.2% isoflurane or with equianesthetic dose of pentobarbital for 4 h. In addition, the authors subjected IL-11 receptor (IL-11R) wild-type, IL-11R-deficient, or IL-11 neutralized mice to 30-min renal ischemia followed by reperfusion under 4 h of anesthesia with pentobarbital or isoflurane (1.2%). RESULTS: Isoflurane increased IL-11 synthesis in human (approximately 300-500% increase, N = 6) and mouse (23 ± 4 [mean ± SD] fold over carrier gas group, N = 4) proximal tubule cells that were attenuated by a TGF-ß1-neutralizing antibody. Mice anesthetized with isoflurane showed significantly increased kidney IL-11 messenger RNA (13.8 ± 2 fold over carrier gas group, N = 4) and protein (31 ± 9 vs. 18 ± 2 pg/mg protein or approximately 80% increase, N = 4) expression compared with pentobarbital-anesthetized mice, and this increase was also attenuated by a TGF-ß1-neutralizing antibody. Furthermore, isoflurane-mediated renal protection in IL-11R wild-type mice was absent in IL-11R-deficient mice or in IL-11R wild-type mice treated with IL-11-neutralizing antibody (N = 4-6). CONCLUSION: In this study, the authors suggest that isoflurane induces renal tubular IL-11 via TGF-ß1 signaling to protect against ischemic acute kidney injury.


Subject(s)
Acute Kidney Injury/prevention & control , Anesthetics, Inhalation/pharmacology , Interleukin-11/physiology , Isoflurane/pharmacology , Acute Kidney Injury/physiopathology , Analysis of Variance , Anesthesia , Animals , Apoptosis/drug effects , Cell Line , Cells, Cultured , Enzyme Induction/drug effects , Humans , Immunohistochemistry , Interleukin-11/biosynthesis , Kidney Tubules, Proximal/drug effects , Kidney Tubules, Proximal/metabolism , Mice , Neutrophil Infiltration/drug effects , Phosphotransferases (Alcohol Group Acceptor)/biosynthesis , Polymerase Chain Reaction , Signal Transduction/drug effects , Transforming Growth Factor beta1/physiology
16.
J Biol Chem ; 288(44): 31655-69, 2013 Nov 01.
Article in English | MEDLINE | ID: mdl-24036113

ABSTRACT

Bovine lactoferricin (LfcinB), a multifunctional peptide, was recently demonstrated to be anti-catabolic and anti-inflammatory in human articular cartilage. LfcinB blocks IL-1-mediated proteoglycan depletion, matrix-degrading enzyme expression, and pro-inflammatory mediator induction. LfcinB selectively activates ERK1/2, p38 (but not JNK), and Akt signaling. However, the relationship between these pathways and LfcinB target genes has never been explored. In this study, we uncovered the remarkable ability of LfcinB in the induction of an anti-inflammatory cytokine, IL-11. LfcinB binds to cell surface heparan sulfate to initiate ERK1/2 signaling and activate AP-1 complexes composed of c-Fos and JunD, which transactivate the IL-11 gene. The induced IL-11 functions as an anti-inflammatory and chondroprotective cytokine in articular chondrocytes. Our data show that IL-11 directly attenuates IL-1-mediated catabolic and inflammatory processes ex vivo and in vitro. Moreover, IL-11 activates STAT3 signaling pathway to critically up-regulate TIMP-1 expression, as a consecutive secondary cellular response after IL-11 induction by LfcinB-ERK-AP-1 axis in human adult articular chondrocytes. The pathological relevance of IL-11 signaling to osteoarthritis is evidenced by significant down-regulation of its cognate receptor expression in osteoarthritic chondrocytes. Together, our results suggest a two-step mechanism, whereby LfcinB induces TIMP-1 through an IL-11-dependent pathway involving transcription factor AP-1 and STAT3.


Subject(s)
Anti-Inflammatory Agents/pharmacology , Chondrocytes/metabolism , Interleukin-11/biosynthesis , Lactoferrin/pharmacology , MAP Kinase Signaling System/drug effects , STAT3 Transcription Factor/metabolism , Up-Regulation/drug effects , Adult , Aged , Animals , Cartilage, Articular , Cattle , Chondrocytes/pathology , Female , Humans , Male , Middle Aged , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3/metabolism , Osteoarthritis/drug therapy , Osteoarthritis/metabolism , Osteoarthritis/pathology , Tissue Inhibitor of Metalloproteinase-1/biosynthesis , Transcription Factor AP-1/metabolism
17.
Cytokine ; 64(1): 138-45, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23911203

ABSTRACT

We previously revealed that endogenous bone morphogenetic protein (Bmp) activity is required for lipid accumulation in 3T3-L1 adipocytes. The present study characterized the role of endogenous Bmp activity in preadipocytes. Endogenous Bmp activity was monitored by analyzing the level of phosphorylation of Smad1/5/8, downstream molecules in the Bmp pathway. Higher levels of phosphorylated Smad1/5/8 were detected in adipogenic cells but not in non-adipogenic cells prior to differentiation induction. The inhibition of the Bmp pathway during this period decreased the expression of Pparγ2 and C/ebpα, which are transcription factors responsible for adipocyte differentiation. The expression of these transcription factors were also down-regulated by Bmp4 knockdown. In addition, endogenous Bmp4 was required for the repression of Intrleukin-11 expression. Endogenous Bmp4 in preadipocytes is indispensable for the onset of the adipogenic program, and may help to maintain the preadipocytic state during adipocyte differentiation.


Subject(s)
Adipocytes/cytology , Adipogenesis , Bone Morphogenetic Protein 4/metabolism , 3T3 Cells , Adipocytes/metabolism , Animals , Bone Morphogenetic Protein 2/antagonists & inhibitors , Bone Morphogenetic Protein 2/metabolism , Bone Morphogenetic Protein 4/biosynthesis , Bone Morphogenetic Protein 4/genetics , CCAAT-Enhancer-Binding Proteins/biosynthesis , CCAAT-Enhancer-Binding Proteins/metabolism , Cattle , Cell Differentiation , Cell Line , Cell Lineage , Interleukin-11/biosynthesis , Mice , PPAR gamma/biosynthesis , PPAR gamma/metabolism , Phosphorylation/drug effects , Pyrazoles/pharmacology , Pyrimidines/pharmacology , Smad1 Protein/metabolism , Smad5 Protein/metabolism , Smad8 Protein/metabolism , Transcriptional Activation/drug effects
18.
J Am Soc Nephrol ; 24(10): 1558-70, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23813214

ABSTRACT

A1 adenosine receptor activation ameliorates ischemic AKI through the induction of renal proximal tubular sphingosine kinase-1. However, systemic adverse effects may limit A1 adenosine receptor-based therapy for ischemic AKI, indicating a need to identify alternative therapeutic targets within this pathway. Here, we evaluated the function of renal proximal tubular IL-11, a clinically approved hematopoietic cytokine, in A1 adenosine receptor-mediated induction of sphingosine kinase-1 and renal protection. Treatment of human proximal tubule epithelial (HK-2) cells with a selective A1 adenosine receptor agonist, chloro-N(6)-cyclopentyladenosine (CCPA), induced the expression of IL-11 mRNA and protein in an extracellular signal-regulated kinase-dependent manner, and administration of CCPA in mice induced renal synthesis of IL-11. Pretreatment with CCPA protected against renal ischemia-reperfusion injury in wild-type mice, but not in IL-11 receptor-deficient mice. Administration of an IL-11-neutralizing antibody abolished the renal protection provided by CCPA. Similarly, CCPA did not induce renal IL-11 expression or protect against renal ischemia-reperfusion injury in mice lacking the renal proximal tubular A1 adenosine receptor. Finally, treatment with CCPA induced sphingosine kinase-1 in HK-2 cells and wild-type mice, but not in IL-11 receptor-deficient or renal proximal tubule A1 adenosine receptor-deficient mice. Taken together, these results suggest that induction of renal proximal tubule IL-11 is a critical intermediary in A1 adenosine receptor-mediated renal protection that warrants investigation as a novel therapeutic target for the treatment of ischemic AKI.


Subject(s)
Acute Kidney Injury/metabolism , Acute Kidney Injury/prevention & control , Interleukin-11/physiology , Receptor, Adenosine A1/physiology , Reperfusion Injury/metabolism , Reperfusion Injury/prevention & control , Adenosine/analogs & derivatives , Adenosine/pharmacology , Adenosine/therapeutic use , Adenosine A1 Receptor Agonists/pharmacology , Adenosine A1 Receptor Agonists/therapeutic use , Animals , Cell Line , Humans , Interleukin-11/biosynthesis , Male , Mice , Mice, Inbred C57BL , Receptor, Adenosine A1/genetics
19.
Asian Pac J Trop Med ; 6(1): 76-8, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23317891

ABSTRACT

OBJECTIVE: To explore the expression of IL-2 and IL-11 and its significance in patients with ankylosing spondylitis (AS). METHODS: A total of 48 active AS patients in our hospital and 40 normal control subjects were selected in our study. Bath ankylosing spondylitis disease activity index (BASDAI), Bath ankylosing spondylitis functional index (BASFI), Bath ankylosing spondylitis metrology index (BASMI), ESR and CRP expression levels were compared before treatment, 12 h after treatment and 24 h after treatment. IL-2 and IL-11 expression were also compared between these two groups. RESULTS: The BASDAI score, BASFI score and BASMI score of the AS patients before treatment significantly decreased compared with those 12 weeks and 24 weeks after treatment (P<0.05). ESR and CRP levels of the AS patients 12 weeks and 24 weeks after treatment significantly decreased compared with those before treatment (P<0.05). Difference was significant in serum IL-2 and IL-11 levels between 12 weeks and 24 weeks after treatment and before treatment (P<0.05). And no statistically significance was observed for serum IL-2 and IL-11 levels between normal control group and those of patients in AS group 24 weeks after treatment (P>0.05). Pearson's linear-correlation analysis showed that serum IL-2 level had a positive correlation with BASDAI, BASFI, BASMI, ESR and CRP (r=0.661,0.547,0.474,0.362,0.416, P<0.05) and serum IL-11 level had a negative correlation with BASDAI, BASFI, BASMI, ESR and CRP (r=-0.629, -0.412, -0.422, -0.387, -0.408, -0.315, P<0.05). CONCLUSIONS: Serum levels of IL-2 in active AS patients significantly increase and will decrease after treatment. However, serum levels of IL-11 significantly decrease and will increase after treatment, which indicates that serum IL-2 has a positive correlation with the degree of AS and serum IL-11 has a negative correlation with the degree of AS, both of which are correlated closely with the onset of AS.


Subject(s)
Interleukin-11/blood , Interleukin-2/blood , Spondylitis, Ankylosing/blood , Adolescent , Adult , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Blood Sedimentation , C-Reactive Protein/metabolism , Case-Control Studies , Female , Humans , Interleukin-11/biosynthesis , Interleukin-2/biosynthesis , Linear Models , Male , Middle Aged , Severity of Illness Index , Spondylitis, Ankylosing/drug therapy
20.
J Oral Sci ; 54(3): 251-9, 2012 Sep.
Article in English | MEDLINE | ID: mdl-23047036

ABSTRACT

Fibroblast growth factor 2 (FGF2) and cyclic AMP (cAMP) play critical roles in controlling the differentiation of osteoblasts and mineralization of bone. We have previously reported that each of FGF2 and forskolin (FSK) alone increase transcription of the bone sialoprotein (BSP) gene, and that together (FGF/FSK) they upregulate BSP gene expression synergistically in rat osteoblast-like ROS 17/2.8 cells. However, other genes that are upregulated after stimulation by FGF2, FSK or FGF/FSK remain unclear. In the present study, we investigated candidate genes associated with mineralization after stimulation by FGF2, FSK and FGF/FSK in two kinds of osteoblast-like cells using microarray and real-time PCR. In ROS17/2.8 cells, FGF2 and FSK each increased the gene expression of c-FOS (7.2-fold and 10.7-fold, respectively). However, FGF/FSK did not induce c-FOS gene expression. FGF2 increased the expression of the dentin matrix protein 1 (DMP1, 129.8-fold) gene. In contrast, FGF/FSK increased the expression of the amphiregulin (AREG, 73-fold) gene maximally. In human osteoblast-like Saos2 cells, FGF2 increased the expression of the osteopontin (SPP1, 16.7-fold), interleukin-8 (IL8, 6.4-fold) and IL11 (4.8-fold) genes. FSK induced the expression of the IL6 (2.6-fold), IL11 (4.0-fold), chemokine ligand 13 (CXCL13, 2.8-fold) and bone morphogenetic protein 2 (BMP2, 2.5-fold) genes. These results suggest that FGF2 and FSK might be crucial regulators of mineralization and bone formation.


Subject(s)
Calcification, Physiologic/genetics , Colforsin/pharmacology , Fibroblast Growth Factor 2/pharmacology , Genes, fos , Osteoblasts/drug effects , Osteoblasts/metabolism , Amphiregulin , Analysis of Variance , Bone Morphogenetic Protein 2/biosynthesis , Bone Morphogenetic Protein 2/genetics , Calcification, Physiologic/drug effects , Cell Differentiation/drug effects , EGF Family of Proteins , Extracellular Matrix Proteins/biosynthesis , Extracellular Matrix Proteins/genetics , Fibroblast Growth Factor 2/physiology , Gene Expression Profiling , Gene Expression Regulation , Glycoproteins/biosynthesis , Glycoproteins/genetics , Intercellular Signaling Peptides and Proteins/biosynthesis , Intercellular Signaling Peptides and Proteins/genetics , Interleukin-11/biosynthesis , Interleukin-11/genetics , Interleukin-6/biosynthesis , Interleukin-6/genetics , Interleukin-8/biosynthesis , Interleukin-8/genetics , Microarray Analysis , Osteopontin/biosynthesis , Osteopontin/genetics , Phosphoproteins/biosynthesis , Phosphoproteins/genetics , Real-Time Polymerase Chain Reaction
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