Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 82
Filter
2.
J Gastroenterol Hepatol ; 35(3): 438-445, 2020 Mar.
Article in English | MEDLINE | ID: mdl-31498492

ABSTRACT

BACKGROUND AND AIM: Demonstration of villous abnormalities is an essential component of diagnosis of celiac disease (CeD) that requires duodenal biopsies. There is a need for non-invasive biomarker(s) that can predict the presence of villous abnormalities. METHODS: Levels of plasma citrulline, plasma intestinal fatty acid binding protein (I-FABP), and serum regenerating gene 1α (Reg1α) were estimated in treatment naïve patients with CeD and controls. The levels of these biomarkers and their cyclical pattern were validated in a predicted model of enteropathy. Optimum diagnostic cut-off values were derived, and the results were further validated in a prospective validation cohort. RESULTS: While level of plasma citrulline was significantly lower, the levels of plasma I-FABP and serum Reg1α were significantly higher in patients with CeD (n = 131) in comparison with healthy (n = 216) and disease controls (n = 133), and their levels reversed after a gluten-free diet (GFD). In the model of predicted enteropathy (n = 70), a sequential decrease and then increase in the level of plasma citrulline was observed; such a sequential change was not observed with I-FABP and Reg1α. The diagnostic accuracy for prediction of presence of villous abnormality was 89% and 78% if citrulline level was  ≤ 30 µM/L and I-FABP levels were ≥ 1100 pg/mL, respectively. The results were validated in a prospective validation cohort (n = 104) with a sensitivity and specificity of 79.5% and 83.1%, respectively, for predicting villous abnormalities of modified Marsh grade > 2 at calculated cut-off values of citrulline and I-FABP. CONCLUSIONS: Plasma citrulline  ≤ 30 µM/L is the most consistent, highly reproducible non-invasive biomarker that can predict the presence of villous abnormality and has the potential for avoiding duodenal biopsies in 78% patients suspected to have CeD.


Subject(s)
Celiac Disease/diagnosis , Celiac Disease/pathology , Citrulline/blood , Fatty Acid-Binding Proteins/blood , Intestinal Mucosa/abnormalities , Lithostathine/blood , Adult , Biomarkers/blood , Cohort Studies , Female , Humans , Intestinal Mucosa/pathology , Male , Predictive Value of Tests , Young Adult
3.
J Leukoc Biol ; 106(3): 513-529, 2019 09.
Article in English | MEDLINE | ID: mdl-31313381

ABSTRACT

Alterations in the composition of the intestinal microbiota have been associated with development of type 1 diabetes (T1D), but little is known about changes in intestinal homeostasis that contribute to disease pathogenesis. Here, we analyzed oral tolerance induction, components of the intestinal barrier, fecal microbiota, and immune cell phenotypes in non-obese diabetic (NOD) mice during disease progression compared to non-obese diabetes resistant (NOR) mice. NOD mice failed to develop oral tolerance and had defective protective/regulatory mechanisms in the intestinal mucosa, including decreased numbers of goblet cells, diminished mucus production, and lower levels of total and bacteria-bound secretory IgA, as well as an altered IEL profile. These disturbances correlated with bacteria translocation to the pancreatic lymph node possibly contributing to T1D onset. The composition of the fecal microbiota was altered in pre-diabetic NOD mice, and cross-fostering of NOD mice by NOR mothers corrected their defect in mucus production, indicating a role for NOD microbiota in gut barrier dysfunction. NOD mice had a reduction of CD103+ dendritic cells (DCs) in the MLNs, together with an increase of effector Th17 cells and ILC3, as well as a decrease of Th2 cells, ILC2, and Treg cells in the small intestine. Importantly, most of these gut alterations precede the onset of insulitis. Disorders in the intestinal mucosa of NOD mice can potentially interfere with the development of T1D due the close relationship between the gut and the pancreas. Understanding these early alterations is important for the design of novel therapeutic strategies for T1D prevention.


Subject(s)
Diabetes Mellitus, Type 1/pathology , Intestinal Mucosa/abnormalities , Animals , Cytokines/metabolism , Dendritic Cells/immunology , Diabetes Mellitus, Type 1/immunology , Disease Progression , Dysbiosis/pathology , Female , Gastrointestinal Microbiome , Immune Tolerance , Inflammation Mediators/metabolism , Intestinal Mucosa/pathology , Lymph Nodes/pathology , Mice, Inbred BALB C , Mice, Inbred NOD , Mucus/metabolism , Pancreas/metabolism , Pancreas/pathology
4.
Nat Rev Dis Primers ; 5(1): 3, 2019 01 10.
Article in English | MEDLINE | ID: mdl-30631077

ABSTRACT

Coeliac disease is an immune-mediated enteropathy against dietary gluten present in wheat, rye and barley and is one of the most common lifelong food-related disorders worldwide. Coeliac disease is also considered to be a systemic disorder characterized by a variable combination of gluten-related signs and symptoms and disease-specific antibodies in addition to enteropathy. The ingestion of gluten leads to the generation of harmful gluten peptides, which, in predisposed individuals, can induce adaptive and innate immune responses. The clinical presentation is extremely variable; patients may have severe gastrointestinal symptoms and malabsorption, extraintestinal symptoms or have no symptoms at all. Owing to the multifaceted clinical presentation, diagnosis remains a challenge and coeliac disease is heavily underdiagnosed. The diagnosis of coeliac disease is achieved by combining coeliac disease serology and small intestinal mucosal histology during a gluten-containing diet. Currently, the only effective treatment for coeliac disease is a lifelong strict gluten-free diet; however, the diet is restrictive and gluten is difficult to avoid. Optimizing diagnosis and care in coeliac disease requires continuous research and education of both patients and health-care professionals.


Subject(s)
Celiac Disease/diet therapy , Celiac Disease/diagnosis , Celiac Disease/physiopathology , Diet, Gluten-Free/methods , Humans , Intestinal Mucosa/abnormalities , Intestinal Mucosa/pathology , Poaceae/adverse effects , Quality of Life/psychology , Risk Factors , Triticum/adverse effects
7.
J Cell Physiol ; 231(2): 436-48, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26174178

ABSTRACT

The intestinal epithelium responds to and transmits signals from the microbiota and the mucosal immune system to insure intestinal homeostasis. These interactions are in part conveyed by epigenetic modifications, which respond to environmental changes. Protein acetylation is an epigenetic signal regulated by histone deacetylases, including Hdac1 and Hdac2. We have previously shown that villin-Cre-inducible intestinal epithelial cell (IEC)-specific Hdac1 and Hdac2 deletions disturb intestinal homeostasis. To determine the role of Hdac1 and Hdac2 in the regulation of IEC function and the establishment of the dual knockout phenotype, we have generated villin-Cre murine models expressing one Hdac1 allele without Hdac2, or one Hdac2 allele without Hdac1. We have also investigated the effect of short-term deletion of both genes in naphtoflavone-inducible Ah-Cre and tamoxifen-inducible villin-Cre(ER) mice. Mice with one Hdac1 allele displayed normal tissue architecture, but increased sensitivity to DSS-induced colitis. In contrast, mice with one Hdac2 allele displayed intestinal architecture defects, increased proliferation, decreased goblet cell numbers as opposed to Paneth cells, increased immune cell infiltration associated with fibrosis, and increased sensitivity to DSS-induced colitis. In comparison to dual knockout mice, intermediary activation of Notch, mTOR, and Stat3 signaling pathways was observed. While villin-Cre(ER) Hdac1 and Hdac2 deletions led to an impaired epithelium and differentiation defects, Ah-Cre-mediated deletion resulted in blunted proliferation associated with the induction of a DNA damage response. Our results suggest that IEC determination and intestinal homeostasis are highly dependent on Hdac1 and Hdac2 activity levels, and that changes in the IEC acetylome may alter the mucosal environment.


Subject(s)
Histone Deacetylase 1/metabolism , Histone Deacetylase 2/metabolism , Intestinal Mucosa/enzymology , Animals , Cell Differentiation/genetics , Cell Differentiation/physiology , Cell Proliferation/genetics , Cell Proliferation/physiology , Colitis/enzymology , Colitis/genetics , Colitis/pathology , DNA Damage , Disease Models, Animal , Epithelial Cells/enzymology , Goblet Cells/cytology , Goblet Cells/enzymology , Histone Deacetylase 1/deficiency , Histone Deacetylase 1/genetics , Histone Deacetylase 2/deficiency , Histone Deacetylase 2/genetics , Homeostasis , Immunity, Mucosal , Intestinal Mucosa/abnormalities , Intestinal Mucosa/cytology , Mice , Mice, 129 Strain , Mice, Inbred C57BL , Mice, Knockout , Receptors, Notch/metabolism , STAT3 Transcription Factor/metabolism , Signal Transduction , TOR Serine-Threonine Kinases/metabolism
8.
World J Gastroenterol ; 20(4): 1123-6, 2014 Jan 28.
Article in English | MEDLINE | ID: mdl-24574788

ABSTRACT

Duodenal duplication cysts are rare congenital anomalies. Duodenal duplication should be considered in the differential diagnosis of patients who present with abdominal symptoms with cystic structures neighboring the duodenum. Here, we present an 8-year-old girl with a duodenal duplication cyst treated with partial cystectomy with mucosal stripping performed laparoscopically. Laparoscopic surgery can be considered as a treatment option for duodenal duplication cysts, especially in extraluminal locations.


Subject(s)
Cysts/surgery , Duodenal Diseases/surgery , Duodenum/surgery , Intestinal Mucosa/surgery , Laparoscopy , Child , Cysts/congenital , Cysts/diagnosis , Duodenal Diseases/congenital , Duodenal Diseases/diagnosis , Duodenum/abnormalities , Duodenum/diagnostic imaging , Female , Humans , Intestinal Mucosa/abnormalities , Intestinal Mucosa/diagnostic imaging , Magnetic Resonance Imaging , Tomography, X-Ray Computed , Treatment Outcome
10.
Turk J Pediatr ; 56(4): 440-3, 2014.
Article in English | MEDLINE | ID: mdl-25818968

ABSTRACT

Tufting enteropathy is an autosomal recessive congenital enteropathy presenting with early-onset severe intractable diarrhea. It presents with watery diarrhea that develops in the first days after birth and persists despite bowel rest. Growth is impaired, and most patients require total parenteral nutrition. The histological characteristic of tufting enteropathy is the presence of epithelial tufts. We hereby present a patient who was referred to our neonatal intensive care unit because of chronic diarrhea and diagnosed with tufting enteropathy according to histological examination. To the best of our knowledge, the newborn case presented here is the first one reported from Turkey. As TE is a very rare disease, it should be considered in patients with continuing diarrhea beginning in the first days of life.


Subject(s)
Diarrhea, Infantile/diagnosis , Intestinal Mucosa/abnormalities , Malabsorption Syndromes/diagnosis , Diagnosis, Differential , Fatal Outcome , Humans , Infant, Newborn , Male , Turkey
13.
J Pediatr Surg ; 46(8): e5-8, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21843709

ABSTRACT

We present a rare case of a male neonate with a perineal mass with rectal mucosa, diagnosed as an exstrophic duplication of the rectum. It was accompanied by a cord that was deeply invested in the pelvic diaphragm and was composed of smooth muscle, fibrous tissue, and some rectal glands. The association of exstrophic rectal duplication with a bifid scrotum, hypospadias, and normal anus has not been described previously in the literature.


Subject(s)
Abnormalities, Multiple/diagnosis , Hypospadias/diagnosis , Intestinal Mucosa/abnormalities , Perineum/abnormalities , Rectum/abnormalities , Scrotum/abnormalities , Abnormalities, Multiple/surgery , Humans , Hypospadias/surgery , Infant, Newborn , Intestinal Mucosa/surgery , Male , Perineum/surgery
15.
J Pediatr Gastroenterol Nutr ; 52(6): 734-9, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21478758

ABSTRACT

BACKGROUND AND OBJECTIVE: Tufting enteropathy (TE) is a congenital abnormality of intestinal mucosa development characterized by severe intestinal failure requiring parenteral nutrition (PN) and, in some cases, small bowel transplantation. A few patients have had a more favorable outcome. The objective of this study was to evaluate possible correlations between histological lesion severity in duodenal biopsies and clinical outcomes in children with TE. PATIENTS AND METHODS: We retrospectively reviewed the records of patients diagnosed with TE between 1993 and 2003 at our institution based on intractable neonatal-onset diarrhea with prolonged dependence on PN and duodenal biopsy findings of villous atrophy, epithelial dysplasia with enterocyte dedifferentiation and disorganization (tufting) of the surface epithelium, and crypt abnormalities. The histological lesions were assessed semiquantitatively and compared with the clinical outcomes including dependence on PN. RESULTS: Seven children, all from consanguineous parents, were studied for a median of 6.5 years. Three were permanently weaned off PN and experienced normal growth without nutritional assistance. Initial biopsies in all 3 children showed severe diffuse histological lesions. At weaning off PN, 2 of these 3 patients had persistent, although less diffuse, histological lesions. CONCLUSIONS: Progressive weaning off PN is possible in some children with TE. In our experience, this favorable outcome was not predicted by histological lesion severity, although the lesions improved in some patients. New biomarkers for identifying the histological lesions and predicting the outcome would be useful.


Subject(s)
Diarrhea/therapy , Duodenum/pathology , Intestinal Diseases/therapy , Intestinal Mucosa/abnormalities , Intestinal Mucosa/pathology , Parenteral Nutrition , Diarrhea/etiology , Female , Humans , Infant , Infant, Newborn , Intestinal Diseases/complications , Intestinal Diseases/pathology , Male , Retrospective Studies , Treatment Outcome
16.
J Clin Invest ; 121(4): 1657-66, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21383503

ABSTRACT

Mucin-type O-linked oligosaccharides (O-glycans) are primary components of the intestinal mucins that form the mucus gel layer overlying the gut epithelium. Impaired expression of intestinal O-glycans has been observed in patients with ulcerative colitis (UC), but its role in the etiology of this disease is unknown. Here, we report that mice with intestinal epithelial cell-specific deficiency of core 1-derived O-glycans, the predominant form of O-glycans, developed spontaneous colitis that resembled human UC, including massive myeloid infiltrates and crypt abscesses. The colitis manifested in these mice was also characterized by TNF-producing myeloid infiltrates in colon mucosa in the absence of lymphocytes, supporting an essential role for myeloid cells in colitis initiation. Furthermore, induced deletion of intestinal core 1-derived O-glycans caused spontaneous colitis in adult mice. These data indicate a causal role for the loss of core 1-derived O-glycans in colitis. Finally, we detected a biosynthetic intermediate typically exposed in the absence of core 1 O-glycan, Tn antigen, in the colon epithelium of a subset of UC patients. Somatic mutations in the X-linked gene that encodes core 1 ß1,3-galactosyltransferase-specific chaperone 1 (C1GALT1C1, also known as Cosmc), which is essential for core 1 O-glycosylation, were found in Tn-positive epithelia. These data suggest what we believe to be a new molecular mechanism for the pathogenesis of UC.


Subject(s)
Colitis/etiology , Polysaccharides/deficiency , Animals , Antigens, Tumor-Associated, Carbohydrate/metabolism , Base Sequence , Colitis/genetics , Colitis/metabolism , Colitis/pathology , Colon/metabolism , DNA Primers/genetics , Disease Models, Animal , Galactosyltransferases/deficiency , Galactosyltransferases/genetics , Humans , Intestinal Mucosa/abnormalities , Intestinal Mucosa/metabolism , Intestinal Mucosa/microbiology , Mice , Mice, 129 Strain , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Molecular Chaperones/genetics , Molecular Chaperones/metabolism , Mutation
17.
Pediatr Neurosurg ; 46(2): 138-40, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20664303

ABSTRACT

Split notochord syndrome is a rare congenital malformation that results in spinal anomalies associated with anomalies of the gastrointestinal tract and central nervous system. This report presents an infrequently described variant of this syndrome. A 9-month-old female presented with a dorsal midline mass since birth which was partially covered by skin, and part of the lesion gave a gross appearance of intestinal mucosa. MRI was suggestive of partial sacral agenesis with spinal dysraphism with tethered cord. Excision of the mass with repair of the spinal defect was done. Histopathology confirmed the presence of gastrointestinal mucosa.


Subject(s)
Intestines/abnormalities , Intestines/surgery , Neural Tube Defects/diagnosis , Notochord/abnormalities , Spinal Dysraphism/diagnosis , Female , Humans , Infant , Intestinal Mucosa/abnormalities , Intestinal Mucosa/surgery , Neural Tube Defects/complications , Neural Tube Defects/surgery , Notochord/surgery , Spinal Dysraphism/complications , Spinal Dysraphism/surgery , Syndrome
18.
Pediatr Dev Pathol ; 13(5): 415-8, 2010.
Article in English | MEDLINE | ID: mdl-20158378

ABSTRACT

We present the 1st case report of an additional enteric smooth muscle layer in a patient with Mowat-Wilson syndrome and Hirschsprung disease. After resection of the aganglionic colon at the age of 5 months, our patient initially suffered from intermittent constipation, and subsequently by the age of 5 years, he developed ongoing diarrhea requiring medical treatment for more than a decade. Although the exact mechanism of abnormal gut motility in this case is unknown, we postulate that the supernumerary muscle and its associated neural plexus may be responsible for the patient's unusual late complication in treated Hirschsprung disease.


Subject(s)
Hirschsprung Disease/pathology , Intestinal Mucosa/abnormalities , Muscle, Smooth/abnormalities , Facies , Humans , Infant, Newborn , Intellectual Disability/pathology , Male , Microcephaly/pathology
SELECTION OF CITATIONS
SEARCH DETAIL
...