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1.

Iodine-129 microdosing for protein and peptide drug development: erythropoietin as a case study.

Lamers, Robert-Jan An; de Jong, Arie Fm; López-Gutiérrez, José M; Gómez-Guzmán, Jm.

Bioanalysis ; 5(1): 53-63, 2013 Jan.

Article in English | MEDLINE | ID: mdl-23256472

ABSTRACT

BACKGROUND: Microdosing is a technique for studying the behavior of compounds in vivo at 1/100th of the dose of a test substance calculated, based on animal data, to yield a pharmacologic effect. In microdosing, use is made of accelerator MS (AMS). In this study, we investigated whether (129)I-labeling of proteins with subsequent AMS measurements is a suitable method to perform microdose studies with therapeutic proteins. We used erythropoietin (EPO) as a case study. RESULTS: In an animal study with (129)I-labeled EPO in Han-Wistar rats, an increase of (129)I-EPO is observed after dose administration. The half-life was found to be 2 and 5.5 h for two different EPOs. These results are in accordance with expected values. CONCLUSION: Although further research is required, (129)I-labeling of proteins seems a feasible method for AMS microdose studies with peptide and protein drugs, such as biosimilars.

Subject(s)

Drug Discovery/methods , Erythropoietin/blood , Mass Spectrometry , Acceleration , Animals , Chromatography, Liquid , Dose-Response Relationship, Drug , Erythropoietin/administration & dosage , Erythropoietin/chemistry , Erythropoietin/pharmacokinetics , Feasibility Studies , Half-Life , Humans , Iodine Isotopes/blood , Iodine Isotopes/chemistry , Male , Rats , Rats, Wistar , Surface Plasmon Resonance

2.

Unsatisfactory biologic behavior of I-fibrinogen labeled by the chloramine-T method.

Metzger, J M; Secker-Walker, R H; Krohn, K A; Welch, M J; Potchen, E J.

J Lab Clin Med ; 82(2): 267-75, 1973 Aug.

Article in English | MEDLINE | ID: mdl-4721380

Subject(s)

Chloramines/blood , Fibrinogen/metabolism , Animals , Chlorides/metabolism , Dogs , Fibrinogen/physiology , Gastric Mucosa/metabolism , Iodine Isotopes/blood , Liver/metabolism , Lung/metabolism , Metabolic Clearance Rate , Methods , Molecular Weight , Myocardium/metabolism , Salivary Glands/metabolism , Scintillation Counting , Spleen/metabolism , Thyroid Gland/metabolism , Time Factors , Trichloroacetic Acid , Urinary Bladder/metabolism

3.

Measurement of serum thyroxine by the Tetralute procedure: the effect of 131 I and 99m Tc administration.

Blum, M; Chandra, R; Drucker, W D.

J Nucl Med ; 14(5): 279-82, 1973 May.

Article in English | MEDLINE | ID: mdl-4698327

Subject(s)

Iodine Isotopes/therapeutic use , Technetium , Thyroxine/blood , Cerebrovascular Disorders/diagnosis , Humans , Hyperthyroidism/blood , Hyperthyroidism/radiotherapy , Iodine Isotopes/blood , Radionuclide Imaging , Technetium/blood , Time Factors

4.

[Role of blood in the transport of fission-product radioisotopes]. / Rol'krovi v transporte nekotorykh oskolochnykh radioizotopov.

Shvydko, N S; Popov, D K; Il'in, L A.

Med Radiol (Mosk) ; 18(3): 65-6, 1973 Mar.

Article in Russian | MEDLINE | ID: mdl-4707402

Subject(s)

Radioisotopes/blood , Yttrium , Animals , Barium/blood , Biological Transport , Cerium Isotopes/blood , Iodine Isotopes/blood , Male , Molybdenum/blood , Praseodymium/blood , Rats , Strontium Isotopes/blood , Tellurium/blood , Yttrium Isotopes/blood

5.

Effects of 60 Co gamma radiation on the distribution, catabolism and incorporation of 125 I-5-iodo-2'-deoxyuridine into intestinal and splenic deoxyribonucleic acid in mice.

Johanson, K J.

Acta Radiol Ther Phys Biol ; 11(6): 556-65, 1972 Dec.

Article in English | MEDLINE | ID: mdl-4661523

Subject(s)

DNA/metabolism , Idoxuridine/radiation effects , Intestine, Small/metabolism , Radiation Effects , Spleen/metabolism , Animals , Ascitic Fluid/metabolism , Chromatography, Thin Layer , Cobalt Isotopes , DNA Replication , Fluorouracil/pharmacology , Idoxuridine/metabolism , Iodine Isotopes/blood , Male , Mice , Thymidine/metabolism

6.

Vascularity and blood flow in x-irradiated Walker carcinoma 256 of rats.

Song, C W; Payne, J T; Levitt, S H.

Radiology ; 104(3): 693-7, 1972 Sep.

Article in English | MEDLINE | ID: mdl-5051488

Subject(s)

Blood Vessels/radiation effects , Carcinoma 256, Walker , Radiation Effects , Animals , Blood Proteins/analysis , Blood Volume , Carcinoma 256, Walker/radiotherapy , Chromium Isotopes , Half-Life , Iodine Isotopes/blood , Male , Methods , Neoplasm Transplantation , Radioisotopes/blood , Rats , Rats, Inbred Strains , Regional Blood Flow/radiation effects , Serum Albumin, Radio-Iodinated , Time Factors , Xenon

7.

[Distribution of 131-iodine in fright thyrotoxic wild rabbits. I]. / 131 Jod-Verteilung bei schreckthyreotoxikotischen Wildkaninchen.

Voss, C; Hartmann, N.

Endokrinologie ; 59(3): 360-70, 1972 May.

Article in German | MEDLINE | ID: mdl-4672328

Subject(s)

Anxiety/metabolism , Hyperthyroidism/metabolism , Iodine Isotopes/metabolism , Animals , Anxiety/complications , Female , Humans , Iodine Isotopes/blood , Kidney/metabolism , Liver/metabolism , Lung/metabolism , Male , Muscles/metabolism , Myocardium/metabolism , Rabbits

8.

Increased vascular permeability induced by staphylococcal alpha-toxin.

Szmigielski, S; Kwarecki, K; Jeljaszewicz, J; Zak, C.

J Pathol ; 106(4): 277-80, 1972 Apr.

Article in English | MEDLINE | ID: mdl-4403038

Subject(s)

Capillary Permeability/drug effects , Staphylococcus , Toxins, Biological/pharmacology , Animals , Anti-Arrhythmia Agents/pharmacology , Brain Chemistry , Guinea Pigs , Histamine H1 Antagonists/pharmacology , Imidazoles/pharmacology , Injections, Intravenous , Intestine, Small/analysis , Iodine Isotopes/analysis , Iodine Isotopes/blood , Kidney/analysis , Lung/analysis , Molecular Weight , Rabbits

9.

The use of thyroid-stimulating hormone-Edecrine pretreatment in the radioiodide therapy of metastatic thyroid cancer. Clinical tolerance and changes in the radioiodine kinetics.

Nemec, J; Silink, K; Neradilová, M; Zamrazil, V; Vána, S; Cenková, V.

Neoplasma ; 19(2): 125-33, 1972.

Article in English | MEDLINE | ID: mdl-4112457

Subject(s)

Ethacrynic Acid/therapeutic use , Iodine Isotopes/therapeutic use , Thyroid Neoplasms/drug therapy , Thyrotropin/therapeutic use , Drug Combinations , Drug Tolerance , Ethacrynic Acid/adverse effects , Hematocrit , Humans , Hypotension/etiology , Iodine Isotopes/blood , Iodine Isotopes/urine , Kinetics , Leukocyte Count , Neoplasm Metastasis , Tetany/etiology , Thyroid Function Tests , Thyrotropin/adverse effects , Time Factors

10.

Modification of chromatographic estimation of labelled iodinated compounds in serum.

Bednár, J; Soutorová, M.

Endocrinol Exp ; 6(4): 203-12, 1972.

Article in English | MEDLINE | ID: mdl-4118446

Subject(s)

Amino Acids/blood , Autoradiography , Chromatography, Paper , Chromatography, Thin Layer , Humans , Indicators and Reagents , Iodine Isotopes/blood , Microchemistry , Thyroid Function Tests , Thyronines/blood , Thyroxine/blood , Tyrosine/blood

11.

Vascular clearance and metabolism of lipid by the reticuloendothelial system in dogs.

Cornell, R P; Saba, T M.

Am J Physiol ; 221(5): 1511-6, 1971 Nov.

Article in English | MEDLINE | ID: mdl-5124296

Subject(s)

Lipid Metabolism , Mononuclear Phagocyte System/metabolism , Phagocytosis , Animals , Dogs , Emulsions , Iodine Isotopes/blood , Liver/metabolism , Lung/metabolism , Spleen/metabolism , Triglycerides/blood , Triglycerides/metabolism , Triolein

12.

Whole-body autoradiography of the distribution of 125 I-thyrocalcitonin in mice.

Hicks, E P; Cooper, C W; Waddell, W J.

J Dent Res ; 50(5): 1307-13, 1971.

Article in English | MEDLINE | ID: mdl-5285790

Subject(s)

Autoradiography , Calcitonin/metabolism , Iodine Isotopes/metabolism , Animals , Bile , Biodegradation, Environmental , Bone and Bones/metabolism , Iodine Isotopes/blood , Iodine Isotopes/urine , Kidney/metabolism , Liver/metabolism , Mice , Submandibular Gland/metabolism , Thyroid Gland/metabolism

13.

131I concentration in submandibular salivary glands of rats and mice.

Stephen, K W; Harden, R M; Robertson, J W.

J Dent Res ; 50(4): 979, 1971.

Article in English | MEDLINE | ID: mdl-5283260

Subject(s)

Iodine Isotopes/analysis , Submandibular Gland/analysis , Animals , Iodine Isotopes/blood , Mice , Rats , Time Factors

14.

Interactions of adrenergic blocking drugs with the in vivo release of thyroid hormone induced by thyrotrophin and the long-acting thyroid stimulator.

Melander, A.

Acta Endocrinol (Copenh) ; 66(1): 151-61, 1971 Jan.

Article in English | MEDLINE | ID: mdl-4395421

Subject(s)

Isoproterenol/antagonists & inhibitors , Long-Acting Thyroid Stimulator/antagonists & inhibitors , Phenoxybenzamine/pharmacology , Phentolamine/pharmacology , Propranolol/pharmacology , Thyrotropin/antagonists & inhibitors , Adrenergic alpha-Antagonists/pharmacology , Adrenergic beta-Antagonists/pharmacology , Animals , Female , Iodine Isotopes/blood , Mice , Thyroid Gland/drug effects , Thyroid Hormones/metabolism

15.

[Method for measuring the speed of blood clearance of a quickly excreted labelled molecule by carotid shunt and extracorporeal circulation in the rat]. / Méthode de mesure de la vitesse de disparition sanguine d'une molécule marquée d'excrétion rapide par dérivation carotidienne et circulation extracorporelle chez le rat.

Ardiet, C; Peyrin, J O.

C R Seances Soc Biol Fil ; 165(5): 1062-6, 1971.

Article in French | MEDLINE | ID: mdl-4261485

Subject(s)

Arteriovenous Shunt, Surgical , Contrast Media/blood , Extracorporeal Circulation , Iodine Isotopes/blood , Iothalamic Acid/blood , Animals , Autoanalysis , Carotid Arteries , Half-Life , Metabolic Clearance Rate , Radioisotope Dilution Technique , Rats

16.

[Kinetics of radioiodine elimination]. / Eliminaciona kinetika radiojoda.

Popovic, S; Simonovic, I; Jovanovic, V.

Arh Hig Rada Toksikol ; 22(3): 221-33, 1971.

Article in Croatian | MEDLINE | ID: mdl-5161431

Subject(s)

Iodine Isotopes/urine , Humans , Iodine Isotopes/blood , Kidney/metabolism , Kinetics , Protein Binding , Thyroid Gland/metabolism

17.

[Characteristics of the biological effect of the thyrotropic activity of the blood of diffuse toxic goiter patients]. / K kharakteristike biologicheskogo deistviia tireotropnoi aktivnosti krovi krovi bol'nykh diffuznym toksicheskim zobom.

Baranov, V G; Potin, V V; Stepanov, G S; Shliakhtina, L G.

Probl Endokrinol (Mosk) ; 16(6): 27-33, 1970.

Article in Russian | MEDLINE | ID: mdl-4106950

Subject(s)

Goiter/blood , Hypothyroidism/blood , Long-Acting Thyroid Stimulator/blood , Animals , Biological Assay , Humans , Iodine Isotopes/blood , Mice , Sodium Chloride , Thyroid Function Tests , Thyrotropin/blood , Time Factors

18.

Composition of nonextractable radioactivity formed after injection of labeled L-thyroxine and 3,5,3'-triiodo-L-thyronine in rats.

Surks, M I; Oppenheimer, J H.

Endocrinology ; 87(3): 567-75, 1970 Sep.

Article in English | MEDLINE | ID: mdl-5424577

Subject(s)

Iodine Isotopes/blood , Liver/radiation effects , Radiation Effects , Thyroxine/pharmacology , Triiodothyronine/pharmacology , Animals , Carbon Isotopes/pharmacology , Iodine Isotopes/pharmacology , Kidney/radiation effects , Muscles/radiation effects , Proteins , Rats

19.

Effect of perchlorate on the milk-plasma radioiodine ratio of intact and thyroidectomized goats.

Djurdjevic, D; Lengemann, F W.

J Dairy Sci ; 53(6): 808-12, 1970 Jun.

Article in English | MEDLINE | ID: mdl-4315915

Subject(s)

Goats/physiology , Iodine Isotopes/metabolism , Mammary Glands, Animal/drug effects , Perchlorates/pharmacology , Thyroid Gland/physiology , Animals , Iodine/metabolism , Iodine Isotopes/blood , Milk

20.

Assessment of function in the transplanted kidney with 131-I hippuran.

Rodriguez-Antunez, A; Gill, W M; Egleston, T A.

J Urol ; 103(5): 574-6, 1970 May.

Article in English | MEDLINE | ID: mdl-4910282

Subject(s)

Kidney Function Tests , Kidney Transplantation , Radionuclide Imaging , Humans , Iodine Isotopes/blood , Iodine Radioisotopes , Iodohippuric Acid , Urography

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