ABSTRACT
BACKGROUND AND OBJECTIVES: The published tissue adequacy requirement of kidney medulla for BK virus allograft nephropathy diagnosis lacks systematic verification and competes against potential increased procedural risks from deeper sampling. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We evaluated whether the presence of kidney medulla improved the diagnostic rate of BK nephropathy in 2244 consecutive biopsy samples from 856 kidney transplants with detailed histologic and virologic results. RESULTS: Medulla was present in 821 samples (37%) and correlated with maximal core length (r=0.35; P<0.001). BK virus allograft nephropathy occurred in 74 (3% overall) but increased to 5% (42 of 821) with medulla compared with 2% (32 of 1423) for cortical samples (P<0.001). Biopsy medulla was associated with infection after comprehensive multivariable adjustment of confounders, including core length, glomerular number, and number of cores (adjusted odds ratio, 1.81; 95% confidence interval, 1.02 to 3.21; P=0.04). In viremic cases (n=275), medulla was associated with BK virus nephropathy diagnosis (39% versus 19% for cortex; P<0.001) and tissue polyomavirus load (Banff polyomavirus score 0.64±0.96 versus 0.33±1.00; P=0.006). Biopsy medulla was associated with BK virus allograft nephropathy using generalized estimating equation (odds ratio, 2.04; 95% confidence interval, 1.05 to 3.96; n=275) and propensity matched score comparison (odds ratio, 2.24; 95% confidence interval, 1.11 to 4.54; P=0.03 for 156 balanced pairs). Morphometric evaluation of Simian virus 40 large T immunohistochemistry found maximal infected tubules within the inner cortex and medullary regions (P<0.001 versus outer cortex). CONCLUSIONS: Active BK virus replication concentrated around the corticomedullary junction can explain the higher detection rates for BK virus allograft nephropathy with deep sampling. The current adequacy requirement specifying targeting medulla can be justified to minimize a missed diagnosis from undersampling.
Subject(s)
BK Virus , Kidney Diseases/diagnosis , Kidney Diseases/pathology , Kidney Medulla/pathology , Polyomavirus Infections/complications , Tumor Virus Infections/complications , Adult , Allografts/pathology , Antigens, Polyomavirus Transforming/analysis , Biopsy/standards , Female , Humans , Kidney Cortex/pathology , Kidney Cortex/virology , Kidney Diseases/virology , Kidney Medulla/virology , Kidney Transplantation , Male , Middle Aged , Prospective Studies , Viral LoadSubject(s)
Herpesvirus 4, Human/metabolism , Kidney Medulla/virology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology , Liver/virology , Lymphoproliferative Disorders/virology , Piperazines/adverse effects , Pyrimidines/adverse effects , Spleen/virology , Antineoplastic Agents/adverse effects , Benzamides , Cell Proliferation , Female , Humans , Imatinib Mesylate , In Situ Hybridization, Fluorescence , Lymphoproliferative Disorders/chemically induced , Lymphoproliferative Disorders/metabolism , Middle Aged , Recurrence , Translocation, GeneticSubject(s)
Kidney/blood supply , Kidney/pathology , Porcine Reproductive and Respiratory Syndrome/pathology , Animals , Arterioles/pathology , Arterioles/virology , Endothelium, Vascular/pathology , Kidney/virology , Kidney Medulla/pathology , Kidney Medulla/virology , Necrosis , Porcine respiratory and reproductive syndrome virus/isolation & purification , Swine , Tunica Intima/pathology , Tunica Media/pathologyABSTRACT
Coronaviruses have a marked tropism for epithelial cells. Entry and release of the porcine transmissible gastroenteritis virus (TGEV) is restricted to apical surfaces of polarized epithelial cells, as we have recently shown (J. W. A. Rossen, C. P. J. Bekker, W. F. Voorhout, G. J. A. M. Strous, A. van der Ende, and P. J. M. Rottier, 1994, J. Virol. 68, 7966-7973). In this paper we analyze the interactions of mouse hepatitis coronavirus A59 (MHV-A59) with polarized murine kidney cells (mTAL) grown on permeable supports. After inoculation from the apical or basolateral side, virus entry was found to take place only through the apical membrane. The virus utilized a protein of the carcinoembryonic antigen family as its receptor. In contrast to TGEV, MHV-A59 was released preferentially from the basolateral plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the basolateral culture fluid as well as by electron microscopical observations. In the mouse, MHV initially replicates in the nasal epithelium before being disseminated throughout the body; the basolateral release of MHV from epithelial cells into the animal's circulation may be the first step in the establishment of a systemic infection.