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ISME J ; 4(3): 346-56, 2010 Mar.
Article in English | MEDLINE | ID: mdl-19956276

ABSTRACT

Korarchaeota constitute a recently proposed and little characterized kingdom of Archaea that might have diverged before the lineages of Crenarchaeota and Euryarchaeota split. To assess the diversity, distribution and abundance of Korarchaeota, we analysed 19 terrestrial hot springs in Hveragerdi and Krysuvik, Iceland, and in Kamchatka, Russia. The springs were 70-97 degrees C with pH 2.5-6.5. Out of 19 springs, 12 tested positive for Korarchaeota with specific primers. A Korarchaeota 16S rDNA library was made from each of these. From the 301 clones sequenced, 87 unique sequences were obtained from Iceland and 33 from Kamchatka. The similarity between Kamchatkan and Icelandic 16S rDNA sequences and that of Candidatus Korarchaeum cryptofilum was

Subject(s)
Biodiversity , Hot Springs/microbiology , Korarchaeota/classification , Korarchaeota/isolation & purification , Cell Count , Cluster Analysis , DNA, Archaeal/chemistry , DNA, Archaeal/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Genes, rRNA , Iceland , Korarchaeota/genetics , Molecular Sequence Data , Phylogeny , RNA, Archaeal/genetics , RNA, Ribosomal, 16S/genetics , Russia , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid
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