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1.
Front Immunol ; 15: 1329013, 2024.
Article in English | MEDLINE | ID: mdl-38665908

ABSTRACT

Introduction: Subgroups of autoantibodies directed against voltage-gated potassium channel (Kv) complex components have been associated with immunotherapy-responsive clinical syndromes. The high prevalence and the role of autoantibodies directly binding Kv remain, however, controversial. Our objective was to determine Kv autoantibody binding requirements and to clarify their contribution to the observed immune response. Methods: Binding epitopes were studied in sera (n = 36) and cerebrospinal fluid (CSF) (n = 12) from a patient cohort positive for Kv1.2 but negative for 32 common neurological autoantigens and controls (sera n = 18 and CSF n = 5) by phospho and deep mutational scans. Autoantibody specificity and contribution to the observed immune response were resolved on recombinant cells, cerebellum slices, and nerve fibers. Results: 83% of the patients (30/36) within the studied cohort shared one out of the two major binding epitopes with Kv1.2-3 reactivity. Eleven percent (4/36) of the serum samples showed no binding. Fingerprinting resolved close to identical sequence requirements for both shared epitopes. Kv autoantibody response is directed against juxtaparanodal regions in peripheral nerves and the axon initial segment in central nervous system neurons and exclusively mediated by the shared epitopes. Discussion: Systematic mapping revealed two shared autoimmune responses, with one dominant Kv1.2-3 autoantibody epitope being unexpectedly prevalent. The conservation of the molecular binding requirements among these patients indicates a uniform autoantibody repertoire with monospecific reactivity. The enhanced sensitivity of the epitope-based (10/12) compared with that of the cell-based detection (7/12) highlights its use for detection. The determined immunodominant epitope is also the primary immune response visible in tissue, suggesting a diagnostic significance and a specific value for routine screening.


Subject(s)
Autoantibodies , Autoimmunity , Immunodominant Epitopes , Kv1.2 Potassium Channel , Humans , Autoantibodies/immunology , Autoantibodies/blood , Kv1.2 Potassium Channel/immunology , Immunodominant Epitopes/immunology , Female , Male , Middle Aged , Adult , Autoantigens/immunology , Epitope Mapping , Animals
2.
Neurology ; 70(20): 1883-90, 2008 May 13.
Article in English | MEDLINE | ID: mdl-18474843

ABSTRACT

OBJECTIVE: To document neurologic, oncologic, and serologic associations of patients in whom voltage-gated potassium channel (VGKC) autoantibodies were detected in the course of serologic evaluation for neuronal, glial, and muscle autoantibodies. METHODS: Indirect immunofluorescence screening of sera from 130,000 patients performed on a service basis for markers of paraneoplastic neurologic autoimmunity identified 80 patients whose IgG bound to the synapse-rich molecular layer of mouse cerebellar cortex in a pattern consistent with VGKC immunoreactivity. Antibody specificity was confirmed in all cases by immunoprecipitation of detergent-solubilized brain synaptic proteins complexed with (125)I-alpha-dendrotoxin. RESULTS: Clinical information was available for 72 patients: 51% women, median age at symptom onset 65 years, and median follow-up period 14 months. Neurologic manifestations were acute to subacute in onset in 71% and multifocal in 46%; 71% had cognitive impairment, 58% seizures, 33% dysautonomia, 29% myoclonus, 26% dyssomnia, 25% peripheral nerve dysfunction, 21% extrapyramidal dysfunction, and 19% brainstem/cranial nerve dysfunction. Creutzfeldt-Jakob disease was a common misdiagnosis (14%). Neoplasms encountered (confirmed histologically in 33%) included 18 carcinomas, 5 adenomas, 1 thymoma, and 3 hematologic malignancies. Hyponatremia was documented in 36%, other organ-specific autoantibodies in 49%, and a co-existing autoimmune disorder in 33% (including thyroiditis 21%, type 1 diabetes mellitus 11%). Benefit was reported for 34 of 38 patients (89%) receiving immunotherapy and was marked in 50%. CONCLUSIONS: The spectrum of neurologic manifestations and neoplasms associated with voltage-gated potassium channel (VGKC) autoimmunity is broader than previously recognized. Evaluation for VGKC antibodies is recommended in the comprehensive autoimmune serologic testing of subacute idiopathic neurologic disorders.


Subject(s)
Autoantibodies/blood , Paraneoplastic Syndromes/immunology , Peripheral Nervous System Diseases/immunology , Shaker Superfamily of Potassium Channels/immunology , Adenoma/complications , Adolescent , Adult , Aged , Aged, 80 and over , Autonomic Nervous System Diseases/etiology , Autonomic Nervous System Diseases/immunology , Basal Ganglia Diseases/etiology , Basal Ganglia Diseases/immunology , Child , Cranial Nerve Diseases/etiology , Cranial Nerve Diseases/immunology , Female , Fluorescent Antibody Technique, Indirect , Hematologic Neoplasms/complications , Humans , Kv1.1 Potassium Channel/immunology , Kv1.2 Potassium Channel/immunology , Kv1.6 Potassium Channel , Male , Middle Aged , Myoclonus/etiology , Myoclonus/immunology , Paraneoplastic Syndromes/complications , Peripheral Nervous System Diseases/etiology , Thymoma/complications , Thymus Neoplasms/complications
3.
Am J Hypertens ; 21(2): 213-8, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18174882

ABSTRACT

BACKGROUND: We have previously demonstrated differences in the gene expression of voltage-gated K v1.X channel alpha-subunits in arteries from Wistar-Kyoto rats (WKYs) and spontaneously hypertensive rats (SHRs). The purpose of this study was to test the hypothesis that these differences are also present at the protein level. METHODS: Proteins were isolated from the aorta, mesenteric (MAs) and tail arteries (TAs) of 12- to 15-week-old male WKY and SHR, and analyzed by immunoblotting. K(v) currents were recorded from MA myocytes by patch clamp methods. RESULTS: Expression of Kv1.2, Kv1.5, and Kv2.1 was higher in MAs but was not different in aortas of SHRs as compared to WKYs. In the TA, expression of Kv1.2 and Kv1.5 was higher while that of Kv2.1 was lower in SHR compared to WKY. In the MA, the larger expression of an 80 kDa species of Kv1.2 in SHRs was associated with a lower expression of a 60 kDa species. Kv2.1 gene expression was larger in MAs from SHRs but not different in TAs. K(v) currents associated with Kv1.X and Kv2.1 channels were both larger in MA myocytes from SHRs but less than expected based upon differences in K(v) alpha-subunit protein expression. CONCLUSIONS: For the MA, K(v) protein expression and current components between WKYs and SHRs were qualitatively consistent, but differences in gene and protein expression were not closely correlated. The higher expression of K(v) subunits in small mesenteric arteries (SMAs) of SHR would tend to maintain normal myogenic activity and vasoconstrictor reserve, and could be viewed as a form of homeostatic remodeling.


Subject(s)
Hypertension/genetics , Hypertension/physiopathology , Potassium Channels, Voltage-Gated/genetics , Potassium Channels, Voltage-Gated/physiology , Animals , Antibody Specificity , CHO Cells , Cricetinae , Cricetulus , Gene Expression/physiology , Humans , Kidney/cytology , Kv1.2 Potassium Channel/genetics , Kv1.2 Potassium Channel/immunology , Kv1.2 Potassium Channel/physiology , Kv1.5 Potassium Channel/genetics , Kv1.5 Potassium Channel/immunology , Kv1.5 Potassium Channel/physiology , Male , Mesenteric Arteries/physiology , Monocytes/physiology , Patch-Clamp Techniques , Potassium Channels, Voltage-Gated/immunology , Rats , Rats, Inbred SHR , Rats, Inbred WKY , Shab Potassium Channels/genetics , Shab Potassium Channels/immunology , Shab Potassium Channels/physiology , Tail/blood supply , Thoracic Arteries/physiology
4.
Brain ; 129(Pt 6): 1570-84, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16613892

ABSTRACT

Autoantibodies to Shaker-type (Kv1) K+ channels are now known to be associated with three syndromes. Peripheral nerve hyperexcitability is the chief manifestation of acquired neuromyotonia; the combination of neuromyotonia with autonomic and CNS involvement is called Morvan's syndrome (MoS); and CNS manifestations without peripheral involvement is called limbic encephalitis (LE). To determine the cellular basis of these clinical manifestations, we immunostained mouse neural tissues with sera from patients with neuromyotonia (n = 10), MoS (n = 2) or LE (n = 5), comparing with specific antibodies to relevant K+ channel subunits. Fourteen of 17 patients' sera were positive for Kv1.1, Kv1.2 or Kv1.6 antibodies by immunoprecipitation of 125I-alpha-dendrotoxin-labelled rabbit brain K+ channels. Most sera (11 out of 17) labelled juxtaparanodes of peripheral myelinated axons, co-localizing with Kv1.1 and Kv1.2. In the CNS, all sera tested (n = 12) co-localized with one or more areas of high Kv1.1, Kv1.2 or Kv1.6 channel expression: 10 out of 12 sera co-localized with Kv1.1 and Kv1.2 at spinal cord juxtaparanodes or cerebellar layers, while 3 out of 12 sera co-localized additionally (n = 2) or exclusively (n = 1) with Kv1.6 subunits in Purkinje cells, motor and hippocampal neurons. However, only sera from LE patients labelled the hippocampal areas that are enriched in excitatory, Kv1.1-positive axon terminals. All sera (17 out of 17) labelled one or more of these Kv1 subunits when expressed at the cell membrane of transfected HeLa cells, but not when they were retained in the endoplasmic reticulum. Again, LE sera labelled Kv1.1 subunits more prominently than did MoS or neuromyotonia sera, suggesting an association between higher Kv1.1 specificity and limbic manifestations. In contrast, neuromyotonia sera bound more strongly to Kv1.2 subunits than to Kv1.1 or Kv1.6. These studies support the hypothesis that antibodies to mature surface membrane-expressed Shaker-type K+ channels cause acquired neuromyotonia, MoS and LE, and suggest that future assays based on immunofluorescence of cells expressing individual Kv1 subunits will prove more sensitive than the immunoprecipitation assay. Although more than one type of antibody is often detectable in individual sera, higher affinity for certain subunits or subunit combinations may determine the range of clinical manifestations.


Subject(s)
Autoantibodies/blood , Autoimmune Diseases/immunology , Isaacs Syndrome/immunology , Limbic Encephalitis/immunology , Shaker Superfamily of Potassium Channels/immunology , Adolescent , Adult , Aged , Animals , Antibody Specificity , Biomarkers/blood , Brain/immunology , Female , HeLa Cells , Hippocampus/immunology , Humans , Kv1.1 Potassium Channel/immunology , Kv1.2 Potassium Channel/immunology , Kv1.6 Potassium Channel , Male , Mice , Middle Aged , Peripheral Nerves/immunology , Presynaptic Terminals/immunology , Spinal Cord/immunology , Syringomyelia/immunology , Transfection
5.
Neurology ; 64(7): 1290-3, 2005 Apr 12.
Article in English | MEDLINE | ID: mdl-15824370

ABSTRACT

A patient with thymoma-associated neuromyotonia and voltage-gated potassium channel (Kv1.2 and Kv1.6) antibodies by immunoprecipitation and rat brain immunolabeling was treated successfully with immunoadsorption and cyclophosphamide. Curiously, glutamic acid decarboxylase antibodies, absent at onset, appeared later. Stiff-person syndrome was absent, but fast blink reflex recovery suggested enhanced brainstem excitability. The range of antibodies produced in thymoma-associated neuromyotonia is richer, and the timing of antibody appearance more complex, than previously suspected.


Subject(s)
Autoimmune Diseases of the Nervous System/immunology , Glutamate Decarboxylase/immunology , Isaacs Syndrome/immunology , Potassium Channels, Voltage-Gated/immunology , Thymoma/complications , Thymus Neoplasms/complications , Adult , Animals , Autoantibodies/immunology , Autoimmune Diseases of the Nervous System/physiopathology , Blinking/immunology , Brain Stem/immunology , Brain Stem/physiopathology , Cyclophosphamide/therapeutic use , Humans , Immunosorbent Techniques , Immunosuppressive Agents/therapeutic use , Isaacs Syndrome/physiopathology , Kv1.2 Potassium Channel/immunology , Kv1.6 Potassium Channel/immunology , Male , Rats , Reflex, Abnormal/immunology , Thymoma/immunology , Thymoma/physiopathology , Thymus Neoplasms/immunology , Thymus Neoplasms/physiopathology , Treatment Outcome , gamma-Aminobutyric Acid/biosynthesis
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