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1.
Int J Mol Sci ; 22(9)2021 May 05.
Article in English | MEDLINE | ID: mdl-34063173

ABSTRACT

Recent studies have suggested that flavonoids such as quercetin and probiotics such as Bifidobacterium bifidum (Bf) and Lactobacillus gasseri (Lg) could play a relevant role in inhibiting colon cancer cell growth. Our study investigated the role of dietary supplementation with microencapsulated probiotics (Bf and Lg) along with quercetin in the development of mouse colorectal cancer (CRC). Methods: Adenomatous polyposis coli/multiple intestinal neoplasia (ApcMin/+) mice were fed a standard diet or the same diet supplemented with microencapsulated probiotics (Bf and Lg strains, 107 CFU/100 g food) or both probiotics strains plus microencapsulated quercetin (15 mg/100 g food) for 73 days. Changes in body and organ weights, energy metabolism, intestinal microbiota, and colon tissue were determined. The expression of genes related to the Wnt pathway was also analyzed in colon samples. Results: Dietary supplementation with microencapsulated probiotics or microencapsulated probiotics plus quercetin reduced body weight loss and intestinal bleeding in ApcMin/+ mice. An improvement in energy expenditure was observed after 8 weeks but not after 10 weeks of treatment. A supplemented diet with microencapsulated Bf and Lg reduced the number of aberrant crypt foci (ACF) and adenomas by 45% and 60%, respectively, whereas the supplementation with Bf, Lg and quercetin decreased the number of ACF and adenomas by 57% and 80%, respectively. Microencapsulated Bf and Lg in combination with quercetin could exert inhibition of the canonical Wnt/ß-catenin signaling pathway in the colon of ApcMin/+ mice Conclusions: The administration of microencapsulated Bf and Lg, individually or in combination with quercetin, inhibits the CRC development in ApcMin/+ mice.


Subject(s)
Adenomatous Polyposis Coli/metabolism , Bifidobacterium bifidum/cytology , Carcinogenesis/pathology , Cells, Immobilized/cytology , Colorectal Neoplasms/pathology , Lactobacillus gasseri/cytology , Quercetin/pharmacology , Animals , Body Weight/drug effects , Carcinogenesis/drug effects , Colon/pathology , Colony Count, Microbial , Colorectal Neoplasms/genetics , Energy Metabolism/drug effects , Feces/microbiology , Feeding Behavior , Gene Expression Regulation, Neoplastic/drug effects , Mice, Inbred C57BL , Occult Blood , Organ Size/drug effects , Probiotics/pharmacology , Wnt Signaling Pathway/drug effects
2.
Microbiol Res ; 205: 88-98, 2017 Dec.
Article in English | MEDLINE | ID: mdl-28942850

ABSTRACT

Lactobacillus rhamnosus DSM 14870 and Lactobacillus gasseri DSM 14869 were previously isolated from the vaginal epithelial cells (VEC) of healthy women and selected for the development of the vaginal EcoVag® probiotic capsules. EcoVag® was subsequently shown to provide long-term cure and reduce relapse of bacterial vaginosis (BV) as an adjunct to antibiotic therapy. To identify genes potentially involved in probiotic activity, we performed genome sequencing and characterization of the two strains. The complete genome analysis of both strains revealed the presence of genes encoding functions related to adhesion, exopolysaccharide (EPS) biosynthesis, antimicrobial activity, and CRISPR adaptive immunity but absence of antibiotic resistance genes. Interesting features of L. rhamnosus DSM 14870 genome include the presence of the spaCBA-srtC gene encoding spaCBA pili and interruption of the gene cluster encoding long galactose-rich EPS by integrases. Unique to L. gasseri DSM 14869 genome was the presence of a gene encoding a putative (1456 amino acid) new adhesin containing two rib/alpha-like repeats. L. rhamnosus DSM 14870 and L. gasseri DSM 14869 showed acidification of the culture medium (to pH 3.8) and a strong adhesion capability to the Caco-2 cell line and VEC. L. gasseri DSM 14869 could produce a thick (40nm) EPS layer and hydrogen peroxide. L. rhamnosus DSM 14870 was shown to produce SpaCBA pili and a 20nm EPS layer, and could inhibit the growth of Gardnerella vaginalis, a bacterium commonly associated with BV. The genome sequences provide a basis for further elucidation of the molecular basis for their probiotic functions.


Subject(s)
Capsules/therapeutic use , Genes, Bacterial/genetics , Lacticaseibacillus rhamnosus/genetics , Lactobacillus gasseri/genetics , Probiotics/therapeutic use , Vagina/microbiology , Vaginosis, Bacterial/drug therapy , Whole Genome Sequencing , Anti-Bacterial Agents , Antibiosis , Bacterial Adhesion/genetics , Bacterial Proteins/genetics , Caco-2 Cells , Chromosomes, Bacterial , DNA Transposable Elements , DNA, Bacterial , Drug Resistance, Bacterial/genetics , Female , Fimbriae, Bacterial/genetics , Gardnerella vaginalis/growth & development , Gene Transfer, Horizontal , Humans , Lactobacillus gasseri/cytology , Lactobacillus gasseri/growth & development , Lacticaseibacillus rhamnosus/cytology , Lacticaseibacillus rhamnosus/growth & development , Membrane Proteins/genetics , Multigene Family
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