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3.
Chirurg ; 82(9): 759-60, 762-4, 766, 2011 Sep.
Article in German | MEDLINE | ID: mdl-21826569

ABSTRACT

Liposuction is one of the most commonly performed procedures in aesthetic surgery. The primary aim is body contouring and not weight reduction. The vast amount of available methods for suctioning subcutaneous fat allows an optimal individual treatment plan, keeping in mind the correct indications. Although liposuction is often offered as a minor and harmless surgery, it is a complex procedure. A thorough training of the surgeon and in-depth knowledge about possible complications is essential. In addition to aesthetic indications liposuction is also a valuable tool in reconstructive surgery. For optimal patient selection the skin elasticity has to be considered during the preoperative assessment. Besides pure volume reduction through liposuction, the regenerative possibilities of adipose tissue are of great interest for basic research and clinical applications. Lipofilling or autologous fat transfer is used for a wide variety of aesthetic and reconstructive procedures. Of special interest is the regenerative and reconstructive potential of adipose-derived stem cells (ADSC).


Subject(s)
Lipectomy/methods , Adipose Tissue/physiopathology , Adipose Tissue/transplantation , Cicatrix/etiology , Cicatrix/prevention & control , Clinical Competence , Esthetics , Graft Survival/physiology , Humans , Lipectomy/adverse effects , Lipectomy/education , Mesenchymal Stem Cell Transplantation , Patient Care Planning , Postoperative Complications/etiology , Postoperative Complications/prevention & control , Precision Medicine , Preoperative Care/methods , Plastic Surgery Procedures/adverse effects , Plastic Surgery Procedures/education , Plastic Surgery Procedures/methods , Regeneration/physiology , Surgery, Plastic/education
4.
Cir. plást. ibero-latinoam ; 34(1): 71-79, ene.-mar. 2008. ilus, tab
Article in Es | IBECS | ID: ibc-64980

ABSTRACT

La obtención de tejido adiposo supone un nuevo y prometedor mercado de trabajo para los cirujanos plásticos, ya que los bancos de tejidos escogerán de forma acertada la grasa como el medio más fácil para obtener fuentes de células madre de alto rendimiento, en la medida en que este tejido es capaz de producir al menos cinco veces más unidades formadores de colonias (UFCs) que la médula ósea. El objetivo del presente trabajo es mostrar lo que se puede esperar del tejido adiposo como origen de células adultas de fracción vacularestromal (FVE), y señalar las mejores áreas del cuerpo humano para ser elegidas como donantes de tejido adiposo, extraído mediante liposucción. Describimos la rutina seguida para la obtención de células de FVE mediante la digestión de las muestras de tejido adiposo humano con colagenasa. En el momento de su recolección, esas células presentaban una viabilidad de 92+/- 1% basada en exclusión por Azul de Trypan. Las células de FVE recontadas después de permanecer48 horas en medio de cultivo de Eagle modificado por Dulbecco(DMEM), dentro de una cámara de Neubauer, tras lo cual el rendimiento medio de las células de FVE fue de 7,2 +/- 1,3 x 103células por mililitro de tejido lipoaspirado. En conclusión, pensamos que supone un desafío en la actualida del mejorar las estrategias para la obtención de células de FVE. Este trabajo, por ahora preliminar, muestra que las células de FVE pueden ser fácilmente obtenidas por medio de lipoaspiración. La comparación entre las diferentes áreas donantes, mostró un rendimiento22% más alto para las células de FVE cuando el tejido adiposo había sido obtenido del tronco, en comparación a cuando lo había sido delos miembros (AU)


The harvest of adipose tissue will be a promising labor marketing for plastic surgeons, since tissue banks will certainly choose fat as the easiest way to obtain a high-yield source of stem cells, as this type of tissue can produce at least five times more colony-forming units (CFUs) than bone marrow extracts. The aim of this study is to show what can be expected from fat tissues as an origin of adult stromal vascular fraction (SVF) cells, and to evaluate the best areas to be elected as donor sites within the human body, all obtained by liposuction. The routine to obtain SVF cells by collagenase digestion of human adipose tissue samples was described. At the time of harvest, these cells displayed a viability of 92+/- 1% based on Try pan Blue exclusion, SVF cells were counted after 48 hours culture in Dulbecco´s modified Eagle medium (DMEM) in a Neuberger counting chamber. The average yield of SVF cells was 7,2 +/- 1,3 x 103 cells per millilitre of liposuctioned tissue. As a conclusion, best strategies to obtain SVF cells are an important challenge nowadays. This study, although preliminary, showed that SVF may be easily obtained From liposuction. Comparison among different donor sites showed a 22% higher yield of SVF cells when fat tissue had been obtained from the trunk regions, when confronted with limbs (AU)


Subject(s)
Stem Cells/physiology , Topography, Medical/methods , Adipose Tissue/cytology , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/ultrastructure , Stem Cells/cytology , Lipectomy/trends , Adipose Tissue , Skinfold Thickness , Mesoderm/cytology , Lipectomy/education , Lipectomy/methods
5.
J Dermatol Surg Oncol ; 14(10): 1055-6, 1988 Oct.
Article in English | MEDLINE | ID: mdl-3170925
6.
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