Detection of Extremely Low Level Ciguatoxins through Monitoring of Lithium Adduct Ions by Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry.

Ciguatera poisoning (CP) is the most common type of marine biotoxin food poisoning worldwide, and it is caused by ciguatoxins (CTXs), thermostable polyether toxins produced by dinoflagellate Gambierdiscus and Fukuyoa spp. It is typically caused by the consumption of large fish high on the food chain that have accumulated CTXs in their flesh. CTXs in trace amounts are found in natural samples, and they mainly induce neurotoxic effects in consumers at concentrations as low as 0.2 µg/kg. The U.S. Food and Drug Administration has established CTX maximum permitted levels of 0.01 µg/kg for CTX1B and 0.1 µg/kg for C-CTX1 based on toxicological data. More than 20 variants of the CTX1B and CTX3C series have been identified, and the simultaneous detection of trace amounts of CTX analogs has recently been required. Previously published works using LC-MS/MS achieved the safety levels by monitoring the sodium adduct ions of CTXs ([M+Na]+ > [M+Na]+). In this study, we optimized a highly sensitive method for the detection of CTXs using the sodium or lithium adducts, [M+Na]+ or [M+Li]+, by adding alkali metals such as Na+ or Li+ to the mobile phase. This work demonstrates that CTXs can be successfully detected at the low concentrations recommended by the FDA with good chromatographic separation using LC-MS/MS. It also reports on the method's new analytical conditions and accuracy using [M+Li]+.

[Determination of 24 elements migration in Yixing clay pottery by inductively coupled plasma mass spectrometry].

OBJECTIVE: To establish an analytical method for determining the migration of 24 elements in Yixing clay pottery in 4% acetic acid simulated solution by inductively coupled plasma mass spectrometry. METHODS: Four types of Yixing clay pottery, including Yixing clay teapot, Yixing clay kettle, Yixing clay pot, and Yixing clay electric stew pot, were immersed in 4% acetic acid as a food simulant for testing. The migration amount of 24 elements in the migration solution was determined using inductively coupled plasma mass spectrometry. RESULTS: Lithium, magnesium, aluminum, iron, and barium elements with a mass concentration of 1000 µg/L; Lead, cadmium, total arsenic, chromium, nickel, copper, vanadium, manganese, antimony, tin, zinc, cobalt, molybdenum, silver, beryllium, thallium, titanium, and strontium elements within 100 µg/L there was a linear relationship within, the r value was between 0.998 739 and 0.999 989. Total mercury at 5.0 µg/L, there was a linear relationship within, the r value of 0.995 056. The detection limit of the elements measured by this method was between 0.5 and 45.0 µg/L, the recovery rate was 80.6%-108.9%, and the relative standard deviation was 1.0%-4.8%(n=6). A total of 32 samples of four types of Yixing clay pottery sold on the market, including teapots, boiling kettles, casseroles, and electric stewing pots, were tested. It was found that the migration of 16 elements, including beryllium, titanium, chromium, nickel, cobalt, zinc, silver, cadmium, antimony, total mercury, thallium, tin, copper, total arsenic, molybdenum, and lead, were lower than the quantitative limit. The element with the highest migration volume teapot was aluminum, magnesium, and barium; The kettle was aluminum and magnesium; Casserole was aluminum, magnesium, and lithium; The electric stew pot was aluminum. CONCLUSION: This method is easy to operate and has high accuracy, providing an effective and feasible detection method for the determination and evaluation of element migration in Yixing clay pottery.

Determination of lithium in the blood of the deceased by inductively coupled plasma mass spectrometry in a case of lithium-ion battery fire.

In this study, a case of lithium-ion battery fire is presented. The blood of the deceased was analyzed for lithium (Li) using ICP-MS (inductively coupled plasma mass spectrometry). When compared to normal individuals in the same region, the deceased had much higher levels of Li in their blood. Therefore, conducting quantitative analyses of Li in the bodies of individuals who die in lithium-ion battery fire can provide valuable information into the specific circumstances surrounding their deaths.

Blood and hoof biodistibution of some trace element (Lithium, Copper, Zinc, Strontium and, Lead) in horse from two different areas of Sicily.

BACKGROUND AND PURPOSE: Biological monitoring of trace element horses is a well-known tool for investigating potential bioaccumulation in urbanized and industrialized geographical areas. Some biomaterials such as hoof are considered as an important indicator of environmental pollution. Hooves can store trace elements for a long time compared to blood and this represents a scientific key to long-term monitoring of exposure to environmental pollutants. In the present study, samples of equine hoof and blood were taken from an experimental group of horses living in an industrialized area of Sicily (Italy) and from a control group of horses housed in a non-industrialized area of Sicily to evaluate the bioaccumulation of different trace elements in different substrates. METHODS: Hoof and blood mineral concentration of Li- Lithium, Cu- Copper, Zn- Zinc, Sr- Strontium and, Pb- Lead was processed by means of Thermo Scientific iCAP-Q ICP-MS spectrometer. Student T-test was applied to evaluate the differences between groups and substrates. Correlation analysis between substrates and hematological parameters was performed. RESULTS: Statistical analysis showed a significant statistical difference for Li (p < 0.0001) and Pb (p < 0.0001) between blood and hoof in both groups. A statistically significant difference was observed for Li (p < 0.0001), Cu(p < 0.01), Zn (p < 0.0001) and Pb (p < 0.0001) between substrates in the experimental group. The statistical analysis showed a significant difference of blood Zn (p < 0.01) and hoof Pb (p < 0.01) concentration between groups. Our results revealed a statistically higher concentration of minerals in the blood substrate than in the hoof in both groups. Li (p < 0.0001) and Zn (p < 0.001) were positively correlated between control and experimental group in blood substrate. No correlations were found (P > .05) in hoof between groups for other elements. A positive correlation with WBC was observed in Cu concentration for hoof substrate (p < 0.02) in control group and for Zn in experimental group (p < 0.02). A positive correlation with HGB and HCT was observed for Cu hoof concentration (p < 0.02) in experimental group and PLT resulted positively correlated (p < 0.02) with Pb blood substrate in control group. CONCLUSIONS: Horses were not overexposed to these elements in the analyzed groups. The study highlights the role of a new bioaccumulation substrate such as the horse hoof identified as an analytical matrix for the monitoring of heavy metal concentrations in domestic animals.

[Fortification of drinking water with lithium to reduce suicide in the population].

Antimanic and phase-prophylactic features of lithium (Li) in subjects with affective disorders has been known for a long while. Furthermore, it has also been proven for decades that - partially due to its aforementioned effects - Li has marked antisuicide properties in subjects with mood disorders. Intriguingly, consistent findings from several studies conducted in the last 15 years suggest that the antisuicide effect of Li can also be detected in those members of the population who consume drinking water with high Li contents (in connection with this, we must note that the level of Li in tap water is several orders of magnitude less than the therapeutic dose of Li). Based on these results, and also taking into the consideration the long-known anti-goiter effect of iodized table salt, some experts suggest considering the enrichment of tap water with microdose Li. This paper paper briefly summarizes our current knowledge on this topic as well as the related clinical and ethical dilemmas.

Effects of Diabetes on Elemental Levels and Nanostructure of Root Canal Dentin.

INTRODUCTION: This study evaluated the effects of diabetes mellitus (DM) on the nanostructure of root canal dentin using high-resolution transmission electron microscopy (HRTEM) and inductively coupled plasma mass spectrometry (ICP-MS). METHODS: Twenty extracted human premolars from diabetic and nondiabetic patients (n = 10 in each group) were decoronated and sectioned horizontally into 40 2-mm-thick dentin discs, with each disc designated for a specific test. ICP-MS was used to determine the different elemental levels of copper, lithium, zinc, selenium, strontium, manganese, and magnesium in diabetic and nondiabetic specimens. HRTEM was used to analyze the shape and quantity of the apatite crystals in diabetic and nondiabetic dentin at the nanostructural level. Statistical analysis was performed using Kolmogorov-Smirnov and Student t test (P < .05). RESULTS: ICP-MS revealed significant differences in trace element concentrations between the diabetic and nondiabetic specimens (P < .05), with lower levels of magnesium, zinc, strontium, lithium, manganese, and selenium (P < .05), and higher levels of copper in diabetic specimens (P < .05). HRTEM revealed that diabetic dentin exhibited a less compact structure with smaller crystallites and significantly more crystals in the 2500 nm2 area (P < .05). CONCLUSION: Diabetic dentin exhibited smaller crystallites and altered elemental levels more than nondiabetic dentin, which could explain the higher root canal treatment failure rate in diabetic patients.

Characterization of trace and heavy metal concentration in groundwater: A case study from a tropical river basin of southern India.

This study investigates the hydrogeochemistry of groundwater samples collected from the Shiriya River Basin (SRB), a tropical watershed located in Kasaragod, Kerala, southern India, with a special focus on trace elements. Fifty-four groundwater samples were collected from deep aquifers, which constitute weathered and fractured granitoids and mafic rocks, and the groundwater is tapped by bore wells from a fractured zone at a depth range of 60-100 m. Concentrations of Sr, Li, Ba, Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Cd, Ag, Au, Te, Pb, Re, and PGEs in groundwater were determined by using Q-ICPMS. Out of the 25 analysed trace elements in groundwater, only Sr (489.6 µg/L), Ba (226 µg/L), Li (11.76 µg/L) Mn (396.8 µg/L), Ni (68 µg/L) and Fe (2438.5 µg/L) show anomalous values. The PGEs and the majority of trace elements show values within the permissible limit. Raman spectral studies reveal the presence of celsian in aquifer rocks and are the source of Ba in groundwater. Further, XRF data of the rocks show a high enrichment of Fe and Mn in mafic dyke, basalt, and syenite, and Ba and Sr in granite, pegmatite, and granitic gneiss. Therefore, this study proved that the source of these elements is geogenic, i.e., they are released from the crystalline aquifer through rock-water interaction under alkaline conditions. The results of this study show that the groundwater of the basin has enough metals such as Na, K, Mg, Ca, Mn, Fe, Co, Cu, and Zn, which are good for health. Nevertheless, a few metals (Fe, Mn, Ba, Sr, Li, Ni) that may exert toxic effects on humans are also present in the groundwater of the SRB. As groundwater is found to be a dependable source of drinking water in such watersheds, a comprehensive study on the hydrogeochemistry of all watersheds in tropical regions is recommended.

The effects of lithium on human red blood cells studied using optical spectroscopy and laser trap.

Lithium has been the treatment of choice for patients with bipolar disorder. However, lithium overdose happens more frequently since it has a very narrow therapeutic range in blood, necessitating investigation of its adverse effects on blood cells. The possible changes that lithium exposure may have on functional and morphological characteristics of human red blood cells (RBCs) have been studied ex vivo using single-cell Raman spectroscopy, optical trapping, and membrane fluorescent probe. The Raman spectroscopy was performed with excitation at 532 nm light, which also results in simultaneous photoreduction of intracellular hemoglobin (Hb). The level of photoreduction of lithium-exposed RBCs was observed to decline with lithium concentration, indicating irreversible oxygenation of intracellular Hb from lithium exposure. The lithium exposure may also have an effect on RBC membrane, which was investigated via optical stretching in a laser trap and the results suggest lower membrane fluidity for the lithium-exposed RBCs. The membrane fluidity of RBCs was further studied using the Prodan generalized polarization method and the results verify the reduction of membrane fluidity upon lithium exposure.

Transfer of anticonvulsants and lithium into amniotic fluid, umbilical cord blood & breast milk: A systematic review & combined analysis.

OBJECTIVE: Data on the ability of anticonvulsants and lithium to enter fetal and newborn circulation has become increasingly available; here we estimated penetration ratios in a series of matrices from combined samples of pregnant/breastfeeding women treated with anticonvulsants or lithium. METHODS: We conducted a systematic literature search in PubMed/EMBASE for studies with concentrations of anticonvulsants/lithium from maternal blood, amniotic fluid, umbilical cord blood and/or breast milk. Penetration ratios were calculated by dividing the concentrations in amniotic fluid, umbilical cord plasma or breast milk by the maternal concentrations. When data from multiple studies were available, we calculated combined penetration ratios, weighting studies' mean by study size. RESULTS: Ninety-one eligible studies for brivaracetam, carbamazepine, clonazepam, ethosuximide, gabapentin, lacosamide, lamotrigine, levetiracetam, lithium, oxcarbazepine, perampanel, phenobarbital, phenytoin, pregabalin, primidone, topiramate, valproate, vigabatrin and zonisamide were identified. For amniotic fluid, the highest penetration ratios were estimated for levetiracetam (mean 3.56, range 1.27-5.85, n = 2) and lowest for valproate (mean 0.11, range 0.02-1.02, n = 57). For umbilical cord plasma, oxcarbazepine had the highest ratio (mean 1.59, range 0.11-4.33, n = 12) with clonazepam having the lowest (mean 0.55, range 0.52-0.59, n = 2). For breast milk, the highest ratios were observed for oxcarbazepine (mean 3.75, range 0.5-7.0, n = 2), whereas the lowest were observed for valproate (mean 0.04, range 0.01-0.22, n = 121). DISCUSSION: We observed substantial variability between anticonvulsants and lithium regarding their ability to enter fetal/newborn circulation. Assessing concentrations of anticonvulsants and lithium in maternal samples can provide a surrogate of fetal/infant exposure, although patterns of concentration-dependent effects for maternal/neonatal safety are lacking.

Insights on metal pollution of a Patagonia watershed: A case study in the lower course of the Negro river, Argentina.

This study evaluated the occurrence and distribution of largely known pollutants (Ag, Cd, Cu, Cr, Hg, Ni, Pb, Pd, and Zn), as well as emerging ones (Li, and V) in the water dissolved fraction, suspended particulate matter, and surface sediments from the lower course of the Negro River, Argentina. There are scarce preceding data on inorganic pollution in the entire watershed and, in the case of the emerging pollutants, there are almost no studies performed worldwide. Sampling was conducted in 2019 at six sampling sites, three of them mostly river dominated and the rest under marine domain. The samples were subjected to an acid digestion in a microwave digester, and analyzed using an Inductively Coupled Plasma Atomic Emission Spectrometer. Results: revealed that Cu, Li, V, and Zn were always on the top four of the highest average metal concentrations in water and sediment fractions. The pollution assessment indicated that the watershed might be exposed to anthropogenic pollution, as over 60% of Cu and Zn, and over 85% of Hg in water dissolved samples from the marine dominated sites were above the maximum recommended values from guidelines. The multivariate analyses characterized the watershed into two clusters, with metals in the sediment fraction mainly contributing to the uppermost sites. Indeed, sedimentary Cu and Zn background enrichment indices pointed out a moderate pollution of the river dominated sites. This study highlights the relevance of an integrative approach in metal pollution evaluation, as the results denoted a progressive deterioration of the watershed, affecting the water quality of the lower course of the Negro River and its adjacent coastal zone. Overall, these results contribute to a more complete evaluation of the potential to fulfill the Sustainable Development Goals, with implications for future treatment strategies to enhance the environmental quality of the area.

Assessment of water quality, trace metal pollution, source apportionment and health risks in the groundwater of Chakwal, Pakistan.

Groundwater quality evaluation is the main concern in the regions like Chakwal where it is major source of water for drinking and irrigation due to low storage capacity of the surface water and lack of proper irrigation system. The aim of the present study was to evaluate various physicochemical parameters (pH, EC, TDS, DO, TA, TH and chlorides) and selected essential/toxic trace metal concentrations (Na, K, Ca, Mg, Sr, Li, Ag, Zn, Fe, Cu, Co, Mn, Cr, Cd, and Pb) in order to explore their distribution, correlation, spatial variations and health risk assessment. Average concentration of some trace metals (Co, Cd and Pb) and physicochemical parameters (EC, TDS, and alkalinity) were found to exceed the national/international standards. Multivariate methods of analysis showed strong associations among Fe-Li-K, Sr-Mg-Ca, Cd-Mn, Cu-Zn, Ag-Co, and Cr-Pb-Na which were significantly contributed by anthropogenic activities. Irrigation water quality index exhibited intermediate suitability of the groundwater for irrigation purpose. Health risk evaluation of the trace metals revealed significant non-carcinogenic risks for Cd, Co and Pb (HQing > 1) especially for children. Similarly, significant carcinogenic risk was found to be associated with Pb and Cr which exceeded the safe limit, suggesting the lifetime carcinogenic risk associated with these metals in the groundwater. The present health risk problems should be considered on top priority and immediate actions should be taken to safeguard the water quality in the study area.

Lithium treatment extends human lifespan: findings from the UK Biobank.

Lithium is a nutritional trace element that is also used pharmacologically for the management of bipolar and related psychiatric disorders. Recent studies have shown that lithium supplementation can extend health and lifespan in different animal models. Moreover, nutritional lithium uptake from drinking water was repeatedly found to be positively correlated with human longevity. By analyzing a large observational aging cohort (UK Biobank, n = 501,461 individuals) along with prescription data derived from the National Health Services (NHS), we here find therapeutic supplementation of lithium linked to decreased mortality (p = 0.0017) of individuals diagnosed with affective disorders. Subsequent multivariate survival analyses reveal lithium to be the strongest factor in regards to increased survival effects (hazard ratio = 0.274 [0.119-0.634 CI 95%, p = 0.0023]), corresponding to 3.641 times lower (95% CI 1.577-8.407) chances of dying at a given age for lithium users compared to users of other anti-psychotic drugs. While these results may further support the use of lithium as a geroprotective supplement, it should be noted that doses applied within the UK Biobank/NHS setting require close supervision by qualified medical professionals.

Buffer zone-based trace elements indicating the impact of human activities on karst urban groundwater.

The tanglesome allocation of landscape types at various spatial dimensions is an important component influencing the quality of groundwater environment in karst cities. Trace elements can be used as indicators of the extent of impact on groundwater which is an effective means of tracing groundwater contamination. In this study, we studied the influence of landscape patterns on trace elements in groundwater of typical karst cities in Southwest China (Guiyang City) on a multi-spatial scale by using multivariate statistical analysis. According to the sampling points, buffer zone scales with different radii (500 m, 1000 m, 1500 m, and 4000 m) were established to quantify the land use model. There are suburban and urban differences in trace element content. The city center has higher levels of trace elements compared to suburban areas, especially Li, Ni, Tl, Cu, Sr, Co, As, and Mn. In addition, the outcomes of the multiple linear regression had shown that the size effect of the association from landscape pattern to trace elements varies with different indicators and parameters. The results of redundancy analysis showed an overall change in trace elements was better interpreted by the landscape pattern of the 1500 m-scale buffer. At the same time, at the 1500 m scale, Ni, Tl, Cu, Co, As, Cr, Sr, Li, and Mn were positively correlated with the urban landscape index (4LPI, 4LSI), influenced by urban anthropogenic activities, while Cd, Zn, and Pb were positively correlated with the cropland landscape index (1AI, 1LPI), influenced by agricultural activities. This study indicates that trace elements are a reliable indicator for tracing groundwater contamination. The buffer zone can reflect the extent of urban impacts on groundwater and provide a new and effective analytical tool for groundwater management.

Elemental Profiling of North-East Indian Tea (Camellia sinensis) by ICP-MS and Assessment of Associated Health Risk.

Tea is a perennial crop that requires acidic soil for better plant growth. Due to the acidic nature of tea-growing soil, metals can be easily absorbed by tea plants from growing medium. Other anthropogenic activities are also the major contributor of element in the tea. This study provided a comprehensive database of 24 elements which were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). Selected 24 elements belong to alkali metal (Li, Rb, Cs), alkaline earth metal (Be, Sr, Ba), transition metal (V, Cr, Mn, Co, Ni, Cu, Zn, Ag, Cd, Hg), basic metal (Al, Ga, Sn, Tl, Pb), metalloid (As), non-metal (Se), and actinide (U). Total 321 drier mouth samples were collected during 2020-2021 from eight different regions (Darjeeling, Terai, Dooars, North Bank, Upper Assam, South Bank, Cachar, and Tripura) of north-east India. No inorganic mercury as well as uranium was detected in any tested tea samples. Mean concentrations of Be, As, Ga, Tl, Li, Se, Cd, Ag, Cs, V, Co, and Pb were at trace level, whereas macro-element mean concentrations were distributed in the manner of Al > Mn > Rb > Ba > Zn > Cu > Sr > Cr > Ni > Sn. Human health risk for non-carcinogenic and carcinogenic metals was also assessed for the studied elements. Hazard quotients (HQs) and hazard index (HI) values (< 1) for non-carcinogenic elements indicated no risk. The incremental lifetime cancer risk (ILCR) values for carcinogenic elements indicated no risk for As, Cd, and Pb and medium level risk for Ni. Study concluded that north-east Indian tea would not pose any health hazard.

Rainwater chemistry composition in Bellsund: Sources of elements and deposition discrepancies in the coastal area (SW Spitsbergen, Svalbard).

Discrepancies in rainfall chemistry in Bellsund were found to be influenced by the orographic barrier and related to the variability in the inflow of air masses as well as to the distance of sampling sites from the sea and thus the extent of sea spray impact. This study covers measurements of rainfall (P) and air temperature (T), physicochemical parameters (pH, specific electrolytic conductivity (SEC), major ions (Cl-, NO3-, SO42-) and elements (Na, Ca, Mg,K), as well as trace elements (i.a. As, Cd, Cr, Fe, Co, Pb, Ni, Zn) and dissolved organic carbon (DOC) in 22 rainfall samples collected in August on the Calypsostranda marine terrace and in the forefield of a land-terminating glacier (NW Wedel Jarlsberg Land). The comparison of chemical parameters in the samples revealed major discrepancies, including statistically significant higher rainwater pH and SEC, and the levels of Ag, As, Bi, Ca, Co, Fe, Li, Mn, Mo, Ni, Pb, Sb, and V, deposited near the seashore (Calypsostranda) than in the glacier forefield. Cluster analysis (CA) showed that elements deposited in lower concentrations at the glacier forefield site came from predominately anthropogenic sources. Conversely, CA results of metals and metalloids deposited on the Calypsostranda marine terrace indicate both natural and anthropogenic sources. A correlation matrix and principal component analysis (PCA) permitted identifying two primary factors affecting rainfall chemistry at each of the study sites. In Calypsostranda, these were the inflow of relatively unpolluted cold air (F1 = 35.1%) and sea spray (F2 = 27.6%), while in the glacier forefield the factors were an orographic barrier (F1 = 37.3%) and the inflow of polluted warm air (F2 = 25.2%).

Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types.

The mobility of chemical elements in the soil-orchid system has been poorly studied. The aim of this study is to evaluate the uptake and mobility of several trace (Li, Ba, Sr, Ag, Hg, and B) and macronutrients (Ca, Mg, and K) in the orchid Anacamptis morio (L.) R.M.Bateman, Pridgeon & M.W.Chase from soils in western Serbia. The sampling sites are characterized by three different bedrock types-cherts, limestones, and serpentines, which are the source of the significant chemical differences in the elemental status of the soil and plant tissues. The four-step Community Bureau of Reference sequential extraction procedure was used to determine the distribution of fractions and predict their potential phytoavailability. The orchid and soil samples were analyzed for total elemental content analysis using ICP-OES. The greatest potential for plant availability was determined for Ba and Sr, representing about 80% of the total soil content. More than 40% of Li in the soils was found to be potentially phytoavailable. Significant correlations were found between the total content of Li, B, and Sr in soils. Between 38 and 60% of Li content and more than 80% of Ba and Sr content were determined to be potentially phytoavailable by sequential analysis. The highest bioconcentration factor (> 1) was determined in the case of B and Sr for all orchid organs, while translocation factor for Li was highest in tubers and leaves. The studied elements were mainly stored in tubers and roots, indicating the exclusion strategy of A. morio as a metal tolerance mechanism. The data obtained showed significant differences in metal content in soils and plants originating from sites with different parent materials, suggesting that bedrock type and associated soil properties are important factors that determine chemical element mobility and uptake.

Interplay of higher plants with lithium pollution: Global trends, meta-analysis, and perspectives.

Lithium (Li) is gaining attention due to rapid rise in modern industries but their ultimate fingerprints on plants are not well established. Herein, we executed a meta-analysis of the existing recent literature investigating the impact of Li sources and levels on plant species under different growth conditions to understand the existing state of knowledge. Toxic effects of Li exposure in plants varies as a function of medium and interestingly, more negative responses are reported in hydroponic media as compared to soil and foliar application. Additionally, toxic effects of Li vary with Li source materials and LiCl more negatively affected plant development parameters such as plant germination (n = 48) and root biomass (n = 57) and recorded highly uptake in plants (n = 78), while LiNO3 has more negative effects on shoot biomass. The Li at <50 mg L-1 concentrations significantly influenced the plant physiological indicators including plant germination and root biomass, while 50-500 mg L-1 Li concentration influence the biochemical parameters. The dose-response relationship (EC50) ranges regarding the exposure medium of Li sources in plant species were observed 24.6-196.7 ppm respectively. The uptake potential of Li is dose-dependent and their translocation/bioaccumulation remains unknown. Future work should include full life cycle studies of the crops to elucidate the bioaccumulation of Li in edible tissues and to investigate possible trophic transfer of Li.