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1.
Sci Rep ; 7(1): 17291, 2017 12 11.
Article in English | MEDLINE | ID: mdl-29230010

ABSTRACT

In the study, 15 K. pneumoniae strains were isolated from the mink experiencing respiratory distress in mideastern Shandong province, China, and the prevalence of K. pneumoniae in the sampled mink was 11.9% (15/126). Fourteen (93.33%) of the 15 K. pneumoniae isolates were identified as serotype K2 and hypermucoviscosity phenotype. The 12 virulence-associated genes of the K. pneumoniae isolates were tested. The prevalence of the wabG gene for the isolates were 100% (15/15), the ureA gene 100% (15/15), the rmpA gene 93.33% (14/15), the aerobactin gene 93.33% (14/15), the uge gene 93.33% (14/15), the IucB gene 80% (12/15) and the ybtA gene 13.33% (2/15). But the other five genes, fim, iroNB, wcaG, alls and kfuBC, gave a negative PCR reaction in the 15 isolates, respectively. The animal experiments using K. pneumoniae-SD-12 and K. pneumoniae-SD-21 demonstrated that the serotype K2 was high virulence for mice and mink. These finding implied there exist potential threat that K. pneumoniae pathogens could transmit to human, especially the fur animal farm workers and residents lived near the fur animal farms. Therefore, the etiology and epidemiological surveillance of K. pneumoniae in mink should be strengthened for people's public health.


Subject(s)
Bacterial Proteins/metabolism , Klebsiella Infections/epidemiology , Klebsiella pneumoniae/pathogenicity , Liver Abscess/epidemiology , Respiration Disorders/epidemiology , Serogroup , Virulence Factors/metabolism , Animals , Bacterial Proteins/genetics , China/epidemiology , Incidence , Klebsiella Infections/genetics , Klebsiella Infections/virology , Liver Abscess/genetics , Liver Abscess/virology , Mice , Mink , Phenotype , Respiration Disorders/genetics , Respiration Disorders/virology , Virulence Factors/genetics
3.
Am J Med Sci ; 339(3): 290-1, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20220339

ABSTRACT

Pyogenic liver abscesses are rarely encountered in HIV-infected patients living outside of temperate climates and are usually polymicrobial in nature, with a majority of the pathogens arising from gastrointestinal flora. We describe the second case of a liver abscess in an HIV-positive individual that was caused by methicillin-resistant Staphylococcus aureus (MRSA), most likely due to a partially treated community-acquired MRSA skin abscess. The liver abscess was successfully managed by percutaneous drainage and intravenous antibiotics. This case underlines the ubiquitous nature of community-acquired MRSA and its possible unusual presentations in immunocompromised hosts.


Subject(s)
HIV Infections/diagnosis , HIV Infections/microbiology , Liver Abscess/diagnosis , Liver Abscess/microbiology , Methicillin-Resistant Staphylococcus aureus , Staphylococcal Infections/diagnosis , Adult , Female , Humans , Liver Abscess/virology , Methicillin-Resistant Staphylococcus aureus/isolation & purification , Staphylococcal Infections/virology
4.
Diagn Microbiol Infect Dis ; 62(1): 1-6, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18486404

ABSTRACT

Hypermucoviscosity, rmpA (regulator of mucoid phenotype), aerobactin (an iron siderophore), kfu (an iron uptake system), allS (associated with allantoin metabolism), and K1/K2 capsules are important virulence determinants in Klebsiella pneumoniae for liver abscesses. We determined the prevalence of these virulence factors of 50 nonrepeat K. pneumoniae isolates recovered from patients with primary liver abscesses who were treated at 2 medical centers in Taiwan. Virulence genes were surveyed by polymerase chain reaction analysis. The prevalence of hypermucoviscosity phenotype, plasmid-born rmpA, aerobactin, kfu, and allS genes revealed 96%, 100%, 100%, 100%, and 100% in 26 capsular K1 isolates; 90%, 100%, 100%, 0%, and 0% in 10 K2 isolates; and 79%, 86%, 93%, 50%, and 0% in 14 non-K1/K2 isolates; respectively. When injected into mice intraperitoneally, regardless of any capsule K serotype, K. pneumoniae isolates with hypermucoviscosity phenotype as well as presence of rmpA and aerobactin genes exhibited high virulence for mouse lethality (LD(50), <10(2) CFU). Without significant difference in the prevalence of expressing hypermucoviscosity phenotype and carriage of rmpA and aerobactin genes, these virulent non-K1/K2 isolates are as capable as K1/K2 isolates of causing primary liver abscesses.


Subject(s)
Bacterial Capsules , Klebsiella pneumoniae/classification , Klebsiella pneumoniae/pathogenicity , Liver Abscess , Virulence Factors/metabolism , Animals , Antigens, Bacterial , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Humans , Hydroxamic Acids/metabolism , Klebsiella Infections/epidemiology , Klebsiella Infections/virology , Klebsiella pneumoniae/isolation & purification , Klebsiella pneumoniae/physiology , Liver Abscess/epidemiology , Liver Abscess/virology , Mice , Phenotype , Polysaccharides, Bacterial , Prevalence , Serotyping , Taiwan/epidemiology , Virulence , Virulence Factors/genetics , Viscosity
6.
South Med J ; 90(3): 347-51, 1997 Mar.
Article in English | MEDLINE | ID: mdl-9076313

ABSTRACT

Hepatitis due to herpes simplex virus (HSV) is unusual in healthy individuals. To date, only 56 cases of HSV hepatitis in adult patients have been reported, including 21 pregnant patients. We describe a 25-year-old white woman in her 30th week of gestation who had progressive acute hepatitis. Histologic examination of the liver biopsy specimen showed diffuse microabscesses involving more than 50% of the hepatic parenchyma, with multiple hepatocytes containing Cowdry type A and ground-glass nuclear inclusions. The diagnosis of herpes hepatitis was confirmed by positive immunoreactivity to HSV antibodies in the tissue sections. Intravenous acyclovir therapy was immediately initiated, and the patient's condition improved dramatically. She then had a normal baby at term. Subsequently, the patient had a second pregnancy and an uncomplicated vaginal delivery without recurrence of the disease. Even though alterations of the humoral and cell-mediated immunity occur during pregnancy, herpes hepatitis is rare in pregnant women. Since the prompt administration of antiviral drugs is a lifesaving measure, we recommend including HSV hepatitis in the differential diagnosis of acute hepatitis in pregnancy.


Subject(s)
Hepatitis, Viral, Human/diagnosis , Herpes Simplex/diagnosis , Pregnancy Complications, Infectious/diagnosis , Acute Disease , Acyclovir/administration & dosage , Acyclovir/therapeutic use , Adult , Antibodies, Viral/analysis , Antibody Formation , Antiviral Agents/administration & dosage , Antiviral Agents/therapeutic use , Biopsy , Diagnosis, Differential , Female , Hepatitis, Viral, Human/drug therapy , Hepatitis, Viral, Human/immunology , Herpes Simplex/drug therapy , Herpes Simplex/immunology , Humans , Immunity, Cellular , Injections, Intravenous , Liver Abscess/virology , Pregnancy , Pregnancy Complications, Infectious/drug therapy , Pregnancy Complications, Infectious/immunology , Pregnancy Outcome , Simplexvirus/immunology
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