Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 39
Filter
Add more filters










Publication year range
1.
J Integr Plant Biol ; 60(9): 878-896, 2018 Sep.
Article in English | MEDLINE | ID: mdl-30047576

ABSTRACT

During the establishment of rhizobia-legume symbiosis, the cytokinin receptor LHK1 (Lotus Histidine Kinase 1) is essential for nodule formation. However, the mechanism by which cytokinin signaling regulates symbiosis remains largely unknown. In this study, an LHK1-interacting protein, LjCZF1, was identified and further characterized. LjCZF1 is a C3HC4-type RING finger protein that is highly conserved in plants. LjCZF1 specifically interacted with LHK1 in yeast two-hybrid, in vitro pull-down and co-immunoprecipitation assays conducted in tobacco. Phosphomimetic mutation of the potential threonine (T167D) phosphorylation site enhanced the interaction between LjCZF1 and LHK1, whereas phosphorylation mutation (T167A) eliminated this interaction. Transcript abundance of LjCZF1 was up-regulated significantly after inoculation with rhizobia. The LORE1 insertion mutant and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9-mediated knockout mutant Lotus japonicus plants demonstrated significantly reduced number of infection threads and nodules. In contrast, plants over-expressing LjCZF1 exhibited increased numbers of infection threads and nodules. Collectively, these data support the notion that LjCZF1 is a positive regulator of symbiotic nodulation, possibly through interaction with LHK1.


Subject(s)
Lotus/metabolism , Plant Proteins/metabolism , Rhizobium/physiology , Root Nodules, Plant/metabolism , Gene Expression Regulation, Plant , Lotus/cytology , Lotus/microbiology , Plant Proteins/genetics , Root Nodules, Plant/cytology , Root Nodules, Plant/microbiology , Signal Transduction/genetics , Signal Transduction/physiology , Symbiosis/genetics , Symbiosis/physiology
2.
Plant J ; 93(2): 297-310, 2018 01.
Article in English | MEDLINE | ID: mdl-29171909

ABSTRACT

Phytophthora palmivora is a devastating oomycete plant pathogen. We found that P. palmivora induces disease in Lotus japonicus and used this interaction to identify cellular and molecular events in response to this oomycete, which has a broad host range. Transcript quantification revealed that Lys12 was highly and rapidly induced during P. palmivora infection. Mutants of Lys12 displayed accelerated disease progression, earlier plant death and a lower level of defence gene expression than the wild type, while the defence program after chitin, laminarin, oligogalacturonide or flg22 treatment and the root symbioses with nitrogen-fixing rhizobia and arbuscular mycorrhiza were similar to the wild type. On the microbial side, we found that P. palmivora encodes an active chitin synthase-like protein, and mycelial growth is impaired after treatment with a chitin-synthase inhibitor. However, wheat germ agglutinin-detectable N-acetyl-glucosamine (GlcNAc) epitopes were not identified when the oomycete was grown in vitro or while infecting the roots. This indicates that conventional GlcNAc-mers are unlikely to be produced and/or accumulate in P. palmivora cell walls and that LYS12 might perceive an unknown carbohydrate. The impact of Lys12 on progression of root rot disease, together with the finding that similar genes are present in other P. palmivora hosts, suggests that LYS12 might mediate a common early response to this pathogen.


Subject(s)
Host-Pathogen Interactions , Lotus/immunology , Phytophthora/physiology , Plant Diseases/immunology , Plant Proteins/metabolism , Signal Transduction , Chitin Synthase/genetics , Chitin Synthase/metabolism , Lotus/cytology , Lotus/microbiology , Lotus/parasitology , Mycorrhizae/physiology , Phytophthora/cytology , Plant Diseases/microbiology , Plant Diseases/parasitology , Plant Proteins/genetics , Plant Roots/immunology , Plant Roots/microbiology , Plant Roots/parasitology , Rhizobium/physiology , Symbiosis
3.
Plant Physiol ; 175(1): 361-375, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28733389

ABSTRACT

Legume mutants have shown the requirement for receptor-mediated cytokinin signaling in symbiotic nodule organogenesis. While the receptors are central regulators, cytokinin also is accumulated during early phases of symbiotic interaction, but the pathways involved have not yet been fully resolved. To identify the source, timing, and effect of this accumulation, we followed transcript levels of the cytokinin biosynthetic pathway genes in a sliding developmental zone of Lotus japonicus roots. LjIpt2 and LjLog4 were identified as the major contributors to the first cytokinin burst. The genetic dependence and Nod factor responsiveness of these genes confirm that cytokinin biosynthesis is a key target of the common symbiosis pathway. The accumulation of LjIpt2 and LjLog4 transcripts occurs independent of the LjLhk1 receptor during nodulation. Together with the rapid repression of both genes by cytokinin, this indicates that LjIpt2 and LjLog4 contribute to, rather than respond to, the initial cytokinin buildup. Analysis of the cytokinin response using the synthetic cytokinin sensor, TCSn, showed that this response occurs in cortical cells before spreading to the epidermis in L. japonicus While mutant analysis identified redundancy in several biosynthesis families, we found that mutation of LjIpt4 limits nodule numbers. Overexpression of LjIpt3 or LjLog4 alone was insufficient to produce the robust formation of spontaneous nodules. In contrast, overexpressing a complete cytokinin biosynthesis pathway leads to large, often fused spontaneous nodules. These results show the importance of cytokinin biosynthesis in initiating and balancing the requirement for cortical cell activation without uncontrolled cell proliferation.


Subject(s)
Cytokinins/biosynthesis , Lotus/genetics , Plant Growth Regulators/biosynthesis , Plant Proteins/metabolism , Rhizobiaceae/physiology , Signal Transduction , Gene Expression Regulation, Developmental/genetics , Gene Expression Regulation, Plant , Lotus/cytology , Lotus/growth & development , Lotus/physiology , Models, Biological , Plant Proteins/genetics , Plant Root Nodulation , Plant Roots/cytology , Plant Roots/genetics , Plant Roots/growth & development , Plant Roots/physiology , Root Nodules, Plant/cytology , Root Nodules, Plant/genetics , Root Nodules, Plant/growth & development , Root Nodules, Plant/physiology , Symbiosis
4.
Plant Biol (Stuttg) ; 19(3): 420-427, 2017 May.
Article in English | MEDLINE | ID: mdl-28135026

ABSTRACT

In the generally bee-pollinated genus Lotus a group of four species have evolved bird-pollinated flowers. The floral changes in these species include altered petal orientation, shape and texture. In Lotus these characters are associated with dorsiventral petal identity, suggesting that shifts in the expression of dorsal identity genes may be involved in the evolution of bird pollination. Of particular interest is Lotus japonicus CYCLOIDEA 2 (LjCYC2), known to determine the presence of papillate conical cells on the dorsal petal in L. japonicus. Bird-pollinated species are unusual in not having papillate conical cells on the dorsal petal. Using RT-PCR at various stages of flower development, we determined the timing of expression in all petal types for the three putative petal identity genes (CYC-like genes) in different species with contrasting floral morphology and pollination syndromes. In bird-pollinated species the dorsal identity gene, LjCYC2, is not expressed at the floral stage when papillate conical cells are normally differentiating in bee-pollinated species. In contrast, in bee-pollinated species, LjCYC2 is expressed during conical cell development. Changes in the timing of expression of the above two genes are associated with modifications in petal growth and lateralisation of the dorsal and ventral petals in the bird-pollinated species. This study indicates that changes in the timing, rather than spatial distribution, of expression likely contribute to the modifications of petal micromorphology and petal size during the transition from bee to bird pollination in Macaronesian Lotus species.


Subject(s)
Birds , Fabaceae/genetics , Flowers/genetics , Pollination , Animals , Bees , Fabaceae/cytology , Fabaceae/physiology , Flowers/cytology , Flowers/growth & development , Gene Expression Regulation, Plant , Lotus/cytology , Lotus/genetics , Lotus/physiology , Plant Cells , Spatio-Temporal Analysis
5.
J Plant Physiol ; 208: 40-46, 2017 Jan.
Article in English | MEDLINE | ID: mdl-27889519

ABSTRACT

Iron is an essential nutrient for legume-rhizobium symbiosis and accumulates abundantly in the nodules. However, the concentration of free iron in the cells is strictly controlled to avoid toxicity. It is known that ferritin accumulates in the cells as an iron storage protein. During nodule senescence, the expression of the ferritin gene, Ljfer1, was induced in Lotus japonicus. We investigated a signal transduction pathway leading to the increase of Ljfer1 in the nodule. The Ljfer1 promoter of L. japonicus contains a conserved Iron-Dependent Regulatory Sequence (IDRS). The expression of Ljfer1 was induced by the application of iron or sodium nitroprusside, which is a nitric oxide (NO) donor. The application of iron to the nodule increased the level of NO. These data strongly suggest that iron-induced NO leads to increased expression of Ljfer1 during the senescence of L. japonicus nodules.


Subject(s)
Ferritins/metabolism , Iron/pharmacology , Lotus/physiology , Mesorhizobium/physiology , Nitric Oxide/metabolism , Gene Expression Regulation, Plant , Genes, Reporter , Iron/metabolism , Lotus/cytology , Lotus/drug effects , Nitric Oxide Donors/pharmacology , Nitroprusside/pharmacology , Plant Proteins/metabolism , Promoter Regions, Genetic/genetics , Root Nodules, Plant/cytology , Root Nodules, Plant/drug effects , Root Nodules, Plant/physiology , Signal Transduction , Symbiosis
6.
Mol Plant Microbe Interact ; 29(12): 950-964, 2016 12.
Article in English | MEDLINE | ID: mdl-27929718

ABSTRACT

Legume plants engage in intimate relationships with rhizobial bacteria to form nitrogen-fixing nodules, root-derived organs that accommodate the microsymbiont. Members of the Nuclear Factor Y (NF-Y) gene family, which have undergone significant expansion and functional diversification during plant evolution, are essential for this symbiotic liaison. Acting in a partially redundant manner, NF-Y proteins were shown, previously, to regulate bacterial infection, including selection of a superior rhizobial strain, and to mediate nodule structure formation. However, the exact mechanism by which these transcriptional factors exert their symbiotic functions has remained elusive. By carrying out detailed functional analyses of Lotus japonicus mutants, we demonstrate that LjNF-YA1 becomes indispensable downstream from the initial cortical cell divisions but prior to nodule differentiation, including cell enlargement and vascular bundle formation. Three affiliates of the SHORT INTERNODES/STYLISH transcription factor gene family, called STY1, STY2, and STY3, are demonstrated to be among likely direct targets of LjNF-YA1, and our results point to their involvement in nodule formation.


Subject(s)
CCAAT-Binding Factor/metabolism , Lotus/genetics , Rhizobium/physiology , Transcriptome , Amino Acid Sequence , CCAAT-Binding Factor/genetics , Cell Differentiation , Chromosome Mapping , Genes, Reporter , Lotus/cytology , Lotus/microbiology , Lotus/physiology , Mutation , Phenotype , Plant Proteins/genetics , Plant Proteins/metabolism , Plant Roots/cytology , Plant Roots/genetics , Plant Roots/microbiology , Plant Roots/physiology , Root Nodules, Plant/cytology , Root Nodules, Plant/genetics , Root Nodules, Plant/microbiology , Root Nodules, Plant/physiology , Sequence Alignment , Symbiosis , Transcription Factors/genetics , Transcription Factors/metabolism
7.
Mol Plant Microbe Interact ; 29(12): 925-937, 2016 12.
Article in English | MEDLINE | ID: mdl-27827003

ABSTRACT

Sinorhizobium fredii HH103-Rifr, a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rifr invade L. burttii roots through infection threads or epidermal cracks, respectively. Infection threads in root hairs were not observed in L. burttii plants inoculated with S. fredii HH103-Rifr. A S. fredii HH103-Rifr nodA mutant failed to nodulate L. burttii, demonstrating that Nod factors are strictly necessary for this crack-entry mode, and a noeL mutant was also severely impaired in L. burttii nodulation, indicating that the presence of fucosyl residues in the Nod factor is symbiotically relevant. However, significant symbiotic impacts due to the absence of methylation or to acetylation of the fucosyl residue were not detected. In contrast S. fredii HH103-Rifr mutants showing lipopolysaccharide alterations had reduced symbiotic capacity, while mutants affected in production of either exopolysaccharides, capsular polysaccharides, or both were not impaired in nodulation. Mutants unable to produce cyclic glucans and purine or pyrimidine auxotrophic mutants formed ineffective nodules with L. burttii. Flagellin-dependent bacterial mobility was not required for crack infection, since HH103-Rifr fla mutants nodulated L. burttii. None of the S. fredii HH103-Rifr surface-polysaccharide mutants gained effective nodulation with L. japonicus.


Subject(s)
Lotus/microbiology , Polysaccharides, Bacterial/metabolism , Sinorhizobium fredii/physiology , Symbiosis , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Host Specificity , Lotus/cytology , Lotus/physiology , Mutation , Phenotype , Plant Root Nodulation , Plant Roots/cytology , Plant Roots/microbiology , Plant Roots/physiology , Polysaccharides, Bacterial/chemistry , Purines/metabolism , Pyrimidines/metabolism , Sinorhizobium fredii/cytology , Sinorhizobium fredii/genetics
8.
Mol Plant Microbe Interact ; 29(7): 584-92, 2016 Jul.
Article in English | MEDLINE | ID: mdl-27183039

ABSTRACT

Legume plants can establish symbiosis with soil bacteria called rhizobia to obtain nitrogen as a nutrient directly from atmospheric N2 via symbiotic nitrogen fixation. Legumes and rhizobia form nodules, symbiotic organs in which fixed-nitrogen and photosynthetic products are exchanged between rhizobia and plant cells. The photosynthetic products supplied to rhizobia are thought to be dicarboxylates but little is known about the movement of dicarboxylates in the nodules. In terms of dicarboxylate transporters, an aluminum-activated malate transporter (ALMT) family is a strong candidate responsible for the membrane transport of carboxylates in nodules. Among the seven ALMT genes in the Lotus japonicus genome, only one, LjALMT4, shows a high expression in the nodules. LjALMT4 showed transport activity in a Xenopus oocyte system, with LjALMT4 mediating the efflux of dicarboxylates including malate, succinate, and fumarate, but not tricarboxylates such as citrate. LjALMT4 also mediated the influx of several inorganic anions. Organ-specific gene expression analysis showed LjALMT4 mRNA mainly in the parenchyma cells of nodule vascular bundles. These results suggest that LjALMT4 may not be involved in the direct supply of dicarboxylates to rhizobia in infected cells but is responsible for supplying malate as well as several anions necessary for symbiotic nitrogen fixation, via nodule vasculatures.


Subject(s)
Dicarboxylic Acid Transporters/genetics , Lotus/genetics , Rhizobium/physiology , Symbiosis , Dicarboxylic Acid Transporters/metabolism , Genes, Reporter , Lotus/cytology , Lotus/metabolism , Molecular Sequence Data , Nitrogen Fixation , Phylogeny , Plant Proteins/genetics , Plant Proteins/metabolism , Rhizobium/cytology , Root Nodules, Plant/cytology , Root Nodules, Plant/metabolism , Root Nodules, Plant/microbiology , Sequence Analysis, DNA
9.
Plant Cell Environ ; 39(2): 393-415, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26297195

ABSTRACT

Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus, we performed an integrated multi-omics analysis with a focus on plant and fungal phospholipid (PL) metabolism and biological significance of lysophosphatidylcholine (LPC). Our results support the role of LPC as a bioactive compound eliciting cellular and molecular response mechanisms in Lotus. Evidence is provided for large interspecific chemical diversity of LPC species among mycorrhizae with related AMF species. Lipid, gene expression and elemental profiling emphasize the Lotus-Glomus intraradices interaction as distinct from other arbuscular mycorrhizal (AM) interactions. In G. intraradices, genes involved in fatty acid (FA) elongation and biosynthesis of unsaturated FAs were enhanced, while in Lotus, FA synthesis genes were up-regulated during AMS. Furthermore, FAS protein localization to mitochondria suggests FA biosynthesis and elongation may also occur in AMF. Our results suggest the existence of interspecific partitioning of PL resources for generation of LPC and novel candidate bioactive PLs in the Lotus-G. intraradices symbiosis. Moreover, the data advocate research with phylogenetically diverse Glomeromycota species for a broader understanding of the molecular underpinnings of AMS.


Subject(s)
Glomeromycota/physiology , Glycerophospholipids/metabolism , Lotus/microbiology , Lysophosphatidylcholines/metabolism , Metabolomics/methods , Mycorrhizae/physiology , Proteomics/methods , Symbiosis , Biosynthetic Pathways/drug effects , Biosynthetic Pathways/genetics , Gene Expression Regulation, Plant/drug effects , Glomeromycota/drug effects , Lipid Metabolism/drug effects , Lipid Metabolism/genetics , Lotus/cytology , Lotus/drug effects , Models, Biological , Mycorrhizae/drug effects , Phosphates/pharmacology , Quantitative Trait, Heritable , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Analysis, RNA , Subcellular Fractions/metabolism , Symbiosis/drug effects , Symbiosis/genetics , Time Factors , Transcription, Genetic/drug effects , Transcriptome/drug effects , Transcriptome/genetics
10.
Mol Plant Microbe Interact ; 28(5): 534-45, 2015 May.
Article in English | MEDLINE | ID: mdl-25761207

ABSTRACT

The calcium/calmodulin-dependent protein kinase CCaMK forms a complex with its phosphorylation target CIP73 (CCaMK-interacting protein of 73 kDa). In this work, a homolog of the animal HSC/HSP70 interacting protein (HIP) was identified as an interacting partner of CIP73 in Lotus japonicus. L. japonicus HIP contains all functional domains characteristic of animal HIP proteins. The C-terminal STI1-like domain of L. japonicus HIP was found to be necessary and sufficient for interaction with CIP73. The interaction between CIP73 and HIP occurred in both the nuclei and cytoplasm in Nicotiana benthamiana leaf cells. The interactions between CIP73 and HIP and between CIP73 and CCaMK could take place simultaneously in the same nuclei. HIP transcripts were detected in all plant tissues tested. As nodule primordia developed into young nodules, the expression of HIP was down-regulated and the HIP transcript level became very low in mature nodules. More nodules were formed in transgenic hairy roots of L. japonicus expressing HIP RNA interference at 16 days postinoculation as compared with the control hairy roots expressing the empty vector. It appears that HIP may play a role as a negative regulator for nodulation.


Subject(s)
Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Gene Expression Regulation, Plant , Lotus/metabolism , Molecular Chaperones/metabolism , Plant Proteins/metabolism , Base Sequence , Calcium-Calmodulin-Dependent Protein Kinases/genetics , Down-Regulation , Genes, Reporter , Lotus/cytology , Lotus/genetics , Molecular Chaperones/genetics , Molecular Sequence Data , Phylogeny , Plant Leaves/cytology , Plant Leaves/genetics , Plant Leaves/metabolism , Plant Proteins/genetics , Plant Root Nodulation , Plants, Genetically Modified , Protein Multimerization , Protein Structure, Tertiary , Root Nodules, Plant/cytology , Root Nodules, Plant/genetics , Root Nodules, Plant/metabolism , Sequence Analysis, DNA , Symbiosis , Nicotiana/cytology , Nicotiana/genetics , Nicotiana/metabolism , Two-Hybrid System Techniques , Ubiquitin
11.
Biosci Biotechnol Biochem ; 78(6): 960-8, 2014.
Article in English | MEDLINE | ID: mdl-25036120

ABSTRACT

The plant steroid hormones brassinosteroids (BRs) play important roles in plant growth and responses to stresses. The up-regulation of pathogen resistance by BR signaling has been analyzed, but the relationship between BR and insect herbivores remains largely unclear. BIL1/BZR1 is a BR master transcription factor known to be involved in the regulation of plant development through work conducted on a gain of function mutation. Here, we analyzed the function of BIL1/BZR1 in response to insect feeding and demonstrated that resistance against thrip feeding was increased in the bil1-1D/bzr1-1D mutant compared to wild-type. We generated Lotus japonicus transgenic plants that over-express the Arabidopsis bil1/bzr1 mutant, Lj-bil1/bzr1-OX. The Lj-bil1/bzr1-OX plants showed increased resistance to thrip feeding. The expression levels of the jasmoninc acid (JA)-inducible VSP genes were increased in both Arabidopsis bil1-1D/bzr1-1D mutants and L. japonicus Lj-bil1/bzr1-OX plants. The resistance to thrip feeding caused by the BIL1/BZR1 gene may involve JA signaling.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/physiology , Brassinosteroids/metabolism , Herbivory , Lotus/physiology , Nuclear Proteins/metabolism , Thysanoptera , Transcription Factors/metabolism , Animals , Arabidopsis/cytology , Arabidopsis/genetics , Arabidopsis Proteins/genetics , DNA-Binding Proteins , Lotus/cytology , Lotus/genetics , Mutation , Nuclear Proteins/genetics , Phenotype , Plant Leaves/cytology , Plant Leaves/genetics , Plants, Genetically Modified , Signal Transduction , Transcription Factors/genetics , Transformation, Genetic
12.
Plant J ; 77(1): 146-59, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24329948

ABSTRACT

For the establishment of an effective root nodule symbiosis, a coordinated regulation of the infection processes between the epidermis and cortex is required. However, it remains unclear whether the symbiotic genes identified so far are involved in epidermal and/or cortical infection, e.g. epidermal and cortical infection thread formation or cortical cell division. To analyze the symbiotic gene requirements of the infection process, we have developed an epidermis-specific expression system (pEpi expression system) and examined the symbiotic genes NFR1, NFR5, NUP85, NUP133, CASTOR, POLLUX, CCaMK, CYCLOPS, NSP1 and NSP2 for involvement in the infection process in the epidermis and cortex. Our study shows that expression of the upstream common symbiosis genes CASTOR, POLLUX, NUP85 and NUP133 in the epidermis is sufficient to induce formation of infection threads and cortical cell division, leading to the development of fully effective nodules. Our system also shows a requirement of CCaMK, CYCLOPS, NSP1 and NSP2 for the entire nodulation process, and the different contributions of NFR1 and NFR5 to cortical infection thread formation. Based on these analyses using the pEpi expression system, we propose a functional model of symbiotic genes for epidermal and cortical infection.


Subject(s)
Calcium/metabolism , Gene Expression Regulation, Plant , Lotus/genetics , Rhizobium/genetics , Symbiosis/genetics , Cell Division , Genes, Reporter , Genetic Vectors , Lotus/cytology , Lotus/microbiology , Lotus/physiology , Models, Biological , Mutation , Nucleotide Motifs , Organ Specificity , Phenotype , Plant Epidermis , Plant Proteins/genetics , Plant Proteins/metabolism , Plant Root Nodulation , Plant Roots/metabolism , Promoter Regions, Genetic/genetics , Rhizobium/physiology , Root Nodules, Plant/genetics
13.
Plant J ; 74(6): 1059-71, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23551340

ABSTRACT

In comparison with the technology platforms developed to localize transcripts and proteins, imaging tools for visualization of metabolite distributions in plant tissues are less well developed and lack versatility. This hampers our understanding of plant metabolism and dynamics. In this study, we demonstrate that desorption electrospray ionization mass spectrometry imaging (DESI-MSI) of tissue imprints on porous Teflon may be used to accurately image the distribution of even labile plant metabolites such as hydroxynitrile glucosides, which normally undergo enzymatic hydrolysis by specific ß-glucosidases upon cell disruption. This fast and simple sample preparation resulted in no substantial differences in the distribution and ratios of all hydroxynitrile glucosides between leaves from wild-type Lotus japonicus and a ß-glucosidase mutant plant that lacks the ability to hydrolyze certain hydroxynitrile glucosides. In wild-type, the enzymatic conversion of hydroxynitrile glucosides and the concomitant release of glucose were easily visualized when a restricted area of the leaf tissue was damaged prior to sample preparation. The gene encoding the first enzyme in hydroxynitrile glucoside biosynthesis in L. japonicus leaves, CYP79D3, was found to be highly expressed during the early stages of leaf development, and the hydroxynitrile glucoside distribution in mature leaves reflected this early expression pattern. The utility of direct DESI-MSI of plant tissue was demonstrated using cryo-sections of cassava (Manihot esculenta) tubers. The hydroxynitrile glucoside levels were highest in the outer cell layers, as verified by LC-MS analyses. The unexpected discovery of a hydroxynitrile-derived di-glycoside shows the potential of DESI-MSI to discover and guide investigations into new metabolic routes.


Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Glucosides/metabolism , Lotus/metabolism , Manihot/metabolism , Sorghum/metabolism , Spectrometry, Mass, Electrospray Ionization/methods , Chromatography, Liquid , Cytochrome P-450 Enzyme System/genetics , Genes, Reporter , Glucosides/chemistry , Hydrolysis , Lotus/chemistry , Lotus/cytology , Lotus/genetics , Manihot/chemistry , Manihot/cytology , Mass Spectrometry , Mutation , Nitriles/chemistry , Nitriles/metabolism , Plant Leaves/chemistry , Plant Leaves/cytology , Plant Leaves/metabolism , Plant Tubers/chemistry , Plant Tubers/cytology , Plant Tubers/metabolism , Promoter Regions, Genetic/genetics , Seedlings/chemistry , Seedlings/cytology , Seedlings/metabolism , Sorghum/chemistry , Spectrometry, Mass, Electrospray Ionization/instrumentation , beta-Glucosidase/metabolism
14.
Methods Mol Biol ; 953: 77-93, 2013.
Article in English | MEDLINE | ID: mdl-23073877

ABSTRACT

Plant cell suspension cultures are widely used in plant biology as a convenient tool for the investigation of a wide range of phenomena, bypassing the structural complexity of the plant organism in toto. The homogeneity of an in vitro cell population, the large availability of material, the high rate of cell growth and the good reproducibility of conditions make suspension-cultured cells suitable for the analysis of complex physiological processes at the cellular and molecular levels. Moreover, plant cell cultures provide a valuable platform for the production of high-value secondary metabolites and other substances of commercial interest. Here we describe how to initiate and maintain plant cell cultures starting from explants obtained from in vitro germinated seedlings. Isolation of protoplasts from plant cell suspension cultures and regeneration of plants via organogenesis and somatic embryogenesis are also presented.


Subject(s)
Cell Culture Techniques/methods , Plant Cells/metabolism , Arabidopsis/cytology , Arabidopsis/growth & development , Biomass , Culture Media , Daucus carota/cytology , Daucus carota/growth & development , Germination , Lotus/cytology , Lotus/growth & development , Organogenesis , Protoplasts , Regeneration , Seeds/cytology , Seeds/growth & development , Glycine max/cytology , Glycine max/growth & development , Sterilization , Suspensions
15.
Biosci Biotechnol Biochem ; 76(12): 2332-4, 2012.
Article in English | MEDLINE | ID: mdl-23221703

ABSTRACT

Recent intensive studies of the model plant Arabidopsis thaliana have revealed the molecular mechanisms underlying circadian rhythms in detail. Results of phylogenetic analyses indicated that some of core clock genes are widely conserved throughout the plant kingdom. For another model plant the legume Lotus japonicus, we have reported that it has a set of putative clock genes highly homologous to A. thaliana. Taking advantage of the L. japonicus hairy root transformation system, in this study we characterized the promoter activity of A. thaliana core clock genes CCA1 and PRR5 in heterologous L. japonicus cells and found that the molecular mechanism of circadian rhythm in L. japonicus is compatible with that of A. thaliana.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Arabidopsis/physiology , Circadian Rhythm/genetics , Lotus/genetics , Lotus/physiology , Transcription Factors/genetics , Culture Techniques , Lotus/cytology , Lotus/growth & development , Promoter Regions, Genetic/genetics , Transformation, Genetic
16.
Development ; 139(21): 3997-4006, 2012 Nov.
Article in English | MEDLINE | ID: mdl-23048184

ABSTRACT

Nodulation is a form of de novo organogenesis that occurs mainly in legumes. During early nodule development, the host plant root is infected by rhizobia that induce dedifferentiation of some cortical cells, which then proliferate to form the symbiotic root nodule primordium. Two classic phytohormones, cytokinin and auxin, play essential roles in diverse aspects of cell proliferation and differentiation. Although recent genetic studies have established how activation of cytokinin signaling is crucial to the control of cortical cell differentiation, the physiological pathways through which auxin might act in nodule development are poorly characterized. Here, we report the detailed patterns of auxin accumulation during nodule development in Lotus japonicus. Our analyses showed that auxin predominantly accumulates in dividing cortical cells and that NODULE INCEPTION, a key transcription factor in nodule development, positively regulates this accumulation. Additionally, we found that auxin accumulation is inhibited by a systemic negative regulatory mechanism termed autoregulation of nodulation (AON). Analysis of the constitutive activation of LjCLE-RS genes, which encode putative root-derived signals that function in AON, in combination with the determination of auxin accumulation patterns in proliferating cortical cells, indicated that activation of LjCLE-RS genes blocks the progress of further cortical cell division, probably through controlling auxin accumulation. Our data provide evidence for the existence of a novel fine-tuning mechanism that controls nodule development in a cortical cell stage-dependent manner.


Subject(s)
Indoleacetic Acids/metabolism , Lotus/cytology , Lotus/metabolism , Root Nodules, Plant/cytology , Root Nodules, Plant/metabolism , Cell Division/physiology , Cytokinins/metabolism , Gene Expression Regulation, Plant , Plant Root Nodulation/genetics , Plant Root Nodulation/physiology
17.
Plant J ; 69(1): 181-92, 2012 Jan.
Article in English | MEDLINE | ID: mdl-21910770

ABSTRACT

Temporally and spatially defined calcium signatures are integral parts of numerous signalling pathways. Monitoring calcium dynamics with high spatial and temporal resolution is therefore critically important to understand how this ubiquitous second messenger can control diverse cellular responses. Yellow cameleons (YCs) are fluorescence resonance energy transfer (FRET)-based genetically encoded Ca(2+) -sensors that provide a powerful tool to monitor the spatio-temporal dynamics of Ca(2+) fluxes. Here we present an advanced set of vectors and transgenic lines for live cell Ca(2+) imaging in plants. Transgene silencing mediated by the cauliflower mosaic virus (CaMV) 35S promoter has severely limited the application of nanosensors for ions and metabolites and we have thus used the UBQ10 promoter from Arabidopsis and show here that this results in constitutive and stable expression of YCs in transgenic plants. To improve the spatial resolution, our vector repertoire includes versions of YCs that can be targeted to defined locations. Using this toolkit, we identified temporally distinct responses to external ATP at the plasma membrane, in the cytosol and in the nucleus of neighbouring root cells. Moreover analysis of Ca(2+) dynamics in Lotus japonicus revealed distinct Nod factor induced Ca(2+) spiking patterns in the nucleus and the cytosol. Consequently, the constructs and transgenic lines introduced here enable a detailed analysis of Ca(2+) dynamics in different cellular compartments and in different plant species and will foster novel approaches to decipher the temporal and spatial characteristics of calcium signatures.


Subject(s)
Arabidopsis/genetics , Calcium/analysis , Fluorescence Resonance Energy Transfer/methods , Lotus/cytology , Adenosine Triphosphate/metabolism , Biosensing Techniques/methods , Calcium/metabolism , Calcium-Binding Proteins/analysis , Caulimovirus/genetics , Cell Membrane/metabolism , Cell Nucleus/metabolism , Cotyledon/genetics , Cotyledon/metabolism , Cytosol/metabolism , Genetic Vectors , Lotus/metabolism , Plant Roots/genetics , Plant Roots/metabolism , Plants, Genetically Modified , Promoter Regions, Genetic , Transgenes
18.
Plant Physiol Biochem ; 49(9): 963-9, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21561784

ABSTRACT

The molecular dialogue occurring prior to direct contact between the fungal and plant partners of arbuscular-mycorrhizal (AM) symbioses begins with the release of fungal elicitors, so far only partially identified chemically, which can activate specific signaling pathways in the host plant. We show here that the activation of MAPK is also induced by exudates of germinating spores of Gigaspora margarita in cultured cells of the non-leguminous species tobacco (Nicotiana tabacum), as well as in those of the model legume Lotus japonicus. MAPK activity peaked about 15 min after the exposure of the host cells to the fungal exudates (FE). FE were also responsible for a rapid and transient increase in free cytosolic Ca(2+) in Nicotiana plumbaginifolia and tobacco cells, and pre-treatment with a Ca(2+)-channel blocker (La(3+)) showed that in these cells, MAPK activation was dependent on the cytosolic Ca(2+) increase. A partial dependence of MAPK activity on the common Sym pathway could be demonstrated for a cell line of L. japonicus defective for LjSym4 and hence unable to establish an AM symbiosis. Our results show that MAPK activation is triggered by an FE-induced cytosolic Ca(2+) transient, and that a Sym genetic determinant acts to modulate the intensity and duration of this activity.


Subject(s)
Complex Mixtures/pharmacology , Glomeromycota/chemistry , Lotus/enzymology , MAP Kinase Signaling System/drug effects , Mitogen-Activated Protein Kinase Kinases/metabolism , Nicotiana/enzymology , Plant Cells/enzymology , Plant Proteins/metabolism , Complex Mixtures/chemistry , Glomeromycota/physiology , Lotus/cytology , Spores, Fungal/chemistry , Spores, Fungal/metabolism , Symbiosis/physiology , Time Factors , Nicotiana/cytology
19.
Biochim Biophys Acta ; 1814(4): 496-504, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21256984

ABSTRACT

Two cDNA clones coding for α-type carbonic anhydrases (CA; EC 4.2.1.1) in the nitrogen-fixing nodules of the model legume Lotus japonicus were identified. Functionality of the full-length proteins was confirmed by heterologous expression in Escherichia coli and purification of the encoded polypeptides. The developmental expression pattern of LjCAA1 and LjCAA2 revealed that both genes code for nodule enhanced carbonic anhydrase isoforms, which are induced early during nodule development. The genes were slightly to moderately down-regulated in ineffective nodules formed by mutant Mesorhizobium loti strains, indicating that these genes may also be involved in biochemical and physiological processes not directly linked to nitrogen fixation/assimilation. The spatial expression profiling revealed that both genes were expressed in nodule inner cortical cells, vascular bundles and central tissue. These results are discussed in the context of the possible roles of CA in nodule carbon dioxide (CO(2)) metabolism.


Subject(s)
Carbonic Anhydrases/metabolism , Lotus/enzymology , Root Nodules, Plant/enzymology , Amino Acid Sequence , Carbonic Anhydrases/chemistry , Carbonic Anhydrases/genetics , DNA, Complementary/genetics , Enzyme Assays , Gene Expression Regulation, Plant , Lotus/cytology , Lotus/genetics , Models, Molecular , Molecular Sequence Data , Phylogeny , Protein Structure, Secondary , RNA, Messenger/genetics , RNA, Messenger/metabolism , Root Nodules, Plant/cytology , Root Nodules, Plant/genetics , Sequence Homology, Amino Acid , Up-Regulation/genetics
20.
Trends Plant Sci ; 15(10): 540-5, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20829094

ABSTRACT

Great advances have been made in our understanding of the host plant's common symbiosis functions, which in legumes mediate intracellular accommodation of both nitrogen-fixing bacteria and arbuscular mycorrhiza (AM) fungi. However, it has become apparent that additional plant genes are required specifically for bacterial entry inside the host root. In this opinion article, we consider Lotus japonicus nap1 and pir1 symbiotic mutants within the context of other deleterious mutations that impair an intracellular accommodation of bacteria but have no impact on the colonization of roots by AM fungi. We highlight a clear delineation of early signaling events during bacterial versus AM symbioses while suggesting a more intricate origin of the plant's ability for intracellular accommodation of bacteria.


Subject(s)
Lotus/microbiology , Lotus/physiology , Lotus/cytology , Lotus/genetics , Medicago truncatula/genetics , Medicago truncatula/microbiology , Medicago truncatula/physiology , Mutation , Mycorrhizae/physiology , Plant Roots/microbiology , Symbiosis
SELECTION OF CITATIONS
SEARCH DETAIL
...