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1.
Hipertensión (Madr., Ed. impr.) ; 25(5): 214-217, sept. 2008. tab, ilus
Article in Spanish | IBECS | ID: ibc-151633

ABSTRACT

El síndrome antifosfolípido primario (SAFP) es una entidad clínica que tiene como manifestación más frecuente desde el punto de vista renal la hipertensión, mientras que el síndrome nefrótico es infrecuente. La prevalencia de estenosis de arteria renal en este síndrome es desconocida, al igual que su evolución y tratamiento. Desde el punto de vista terapéutico, en el SAFP está indicada la anticoagulación con dicumorínicos para lograr una ratio normalizada internacional (INR) > 3 para evitar que progresen los eventos trombóticos y la enfermedad renal. A continuación presentamos el caso de un varón de 30 años que diagnosticamos de síndrome antifosfolípido primario que a pesar de presentar una trombosis de arteria renal presentó un ligero deterioro de la función renal sin hipertensión (AU)


Primary antiphospholipid syndrome (PAPS) is a clinical condition whose most frequent manifestation from the renal point of view is hypertension, nephrotic syndrome being a rare presentation form. The prevalence of renal artery stenosis in this syndrome as well as its evolution and treatment are unknown. From the therapeutic point of view, anticoagulation with dicumorinics is indicated in PAPS. An attempt should be made to obtain an international normalized ratio (INR) > 3 to prevent progression of the thrombotic events and renal disease. We present the case of a 30-year old male diagnosed of primary antiphospholipid syndrome. In spite of having renal artery thrombosis, he had a mild deterioration of renal function without hypertension (AU)


Subject(s)
Humans , Male , Female , Renal Artery Obstruction/blood , Renal Artery Obstruction/metabolism , Nephrotic Syndrome/complications , Nephrotic Syndrome/pathology , Antiphospholipid Syndrome/metabolism , Lupus Vulgaris/metabolism , Arthritis, Rheumatoid/metabolism , Arthritis, Rheumatoid/pathology , Hypertension/pathology , Renal Artery Obstruction/complications , Renal Artery Obstruction/diagnosis , Nephrotic Syndrome/diagnosis , Nephrotic Syndrome/metabolism , Antiphospholipid Syndrome/classification , Lupus Vulgaris/complications , Arthritis, Rheumatoid/complications , Arthritis, Rheumatoid/diagnosis , Hypertension/diagnosis
2.
Am J Pathol ; 168(6): 1779-92, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16723695

ABSTRACT

Antibodies to dsDNA represent a classification criterion for systemic lupus erythematosus. Subpopulations of these antibodies are involved in lupus nephritis. No known marker separates nephritogenic from non-nephritogenic anti-dsDNA antibodies. It is not clear whether specificity for glomerular target antigens or intrinsic antibody-affinity for dsDNA or nucleosomes is a critical parameter. Furthermore, it is still controversial whether glomerular target antigen(s) is constituted by nucleosomes or by non-nucleosomal glomerular structures. Previously, we have demonstrated that antibodies eluted from murine nephritic kidneys recognize nucleosomes, but not other glomerular antigens. In this study, we determined the structures that bind nephritogenic autoantibodies in vivo by transmission electron microscopy, immune electron microscopy, and colocalization immune electron microscopy using experimental antibodies to dsDNA, to histones and transcription factors, or to laminin. The data obtained are consistent and point at glomerular basement membrane-associated nucleosomes as target structures for the nephritogenic autoantibodies. Terminal deoxynucleotidyl-transferase-mediated dUTP nick end-labeling or caspase-3 assays demonstrate that lupus nephritis is linked to intraglomerular cell apoptosis. The data suggest that nucleosomes are released by apoptosis and associate with glomerulus basement membranes, which may then be targeted by pathogenic anti-nucleosome antibodies. Thus, apoptotic nucleosomes may represent both inducer and target structures for nephritogenic autoantibodies in systemic lupus erythematosus.


Subject(s)
Apoptosis , Basement Membrane/chemistry , Kidney Glomerulus/metabolism , Lupus Vulgaris/immunology , Nephritis/metabolism , Animals , Autoantibodies/chemistry , Basement Membrane/embryology , Cattle , Histones/metabolism , Immunoglobulin G/chemistry , Kidney/metabolism , Lupus Vulgaris/metabolism , Mice , Mice, Inbred BALB C , Time Factors
3.
J Clin Invest ; 116(4): 961-73, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16585962

ABSTRACT

Antibodies against citrullinated proteins are specific and predictive markers for rheumatoid arthritis although the pathologic relevance of these antibodies remains unclear. To investigate the significance of these autoantibodies, collagen-induced arthritis (CIA) in mice was used to establish an animal model of antibody reactivity to citrullinated proteins. DBA/1J mice were immunized with bovine type II collagen (CII) at days 0 and 21, and serum was collected every 7 days for analysis. Antibodies against both CII and cyclic citrullinated peptide, one such citrullinated antigen, appeared early after immunization, before joint swelling was observed. Further, these antibodies demonstrated specific binding to citrullinated filaggrin in rat esophagus by indirect immunofluorescence and citrullinated fibrinogen by Western blot. To evaluate the role of immune responses to citrullinated proteins in CIA, mice were tolerized with a citrulline-containing peptide, followed by antigen challenge with CII. Tolerized mice demonstrated significantly reduced disease severity and incidence compared with controls. We also identified novel murine monoclonal antibodies specific to citrullinated fibrinogen that enhanced arthritis when coadministered with a submaximal dose of anti-CII antibodies and bound targets within the inflamed synovium of mice with CIA. These results demonstrate that antibodies against citrullinated proteins are centrally involved in the pathogenesis of autoimmune arthritis.


Subject(s)
Antibodies, Monoclonal/immunology , Arthritis, Experimental/immunology , Arthritis, Rheumatoid/immunology , Autoantibodies/biosynthesis , Peptides, Cyclic/immunology , Animals , Antibodies, Monoclonal/metabolism , Antibody Specificity , Antigen-Antibody Reactions , Arthritis, Experimental/microbiology , Arthritis, Rheumatoid/metabolism , Cattle , Collagen , Fibrinogen/immunology , Fibrinogen/metabolism , Fibrinogen/pharmacology , Lupus Vulgaris/immunology , Lupus Vulgaris/metabolism , Male , Mice , Mice, Inbred DBA , Synovial Membrane/immunology , Synovial Membrane/metabolism , Synovial Membrane/pathology , Time Factors
4.
Article in English | MEDLINE | ID: mdl-17369632

ABSTRACT

In most real-life gene expression data sets, there are often multiple sample classes with ordinals, which are categorized into the normal or diseased type. The traditional feature or attribute selection methods consider multiple classes equally without paying attention to the up/down regulation across the normal and diseased types of classes, while the specific gene selection methods particularly consider the differential expressions across the normal and diseased, but ignore the existence of multiple classes. In this paper, for improving the biomarker discovery, we propose to make the best use of these two aspects: the differential expressions (that can be viewed as the domain knowledge of gene expression data) and the multiple classes (that can be viewed as a kind of data set characteristic). Therefore, we simultaneously take into account these two aspects by employing the 1-rank generalized matrix approximations (GMA). Our results show that the consideration of both aspects can not only improve the accuracy of classifying the samples, but also provide a visualization method to effectively analyze the gene expression data on both genes and samples. Based on the GMA mechanism, we further propose an algorithm for obtaining the compact biomarker by reducing the redundancy.


Subject(s)
Biomarkers, Tumor/metabolism , Biomarkers/metabolism , Computational Biology/methods , Gene Expression Profiling/methods , Gene Expression Regulation, Leukemic , Gene Expression Regulation , Algorithms , Cell Line, Tumor , Cluster Analysis , Genomics , Humans , Lupus Vulgaris/metabolism , Models, Genetic , Models, Statistical , Oligonucleotide Array Sequence Analysis
5.
J Proteome Res ; 4(6): 2032-42, 2005.
Article in English | MEDLINE | ID: mdl-16335948

ABSTRACT

The baseline level of gene expression varies between healthy controls and systemic lupus erythematosus (SLE) patients, and among SLE patients themselves. These variations may explain the different clinical manifestations and severity of disease observed in SLE. Epigenetic mechanisms, which involve DNA and histone modifications, are predictably associated with distinct transcriptional states. To understand the interplay between various histone modifications, including acetylation and methylation, and lupus disease, we performed differential expression histone modification analysis in splenocytes from the MRL-lpr/lpr mouse model of lupus. Using stable isotope labeling in combination with mass spectrometry, we found global site-specific hypermethylation (except H3 K4 methylation) and hypoacetylation in histone H3 and H4 MRL-lpr/lpr mice compared to control MRL/MPJ mice. Moreover, we have identified novel histone modifications such as H3 K18 methylation, H4 K31 methylation, and H4 K31 acetylation that are differentially expressed in MRL-lpr/lpr mice compared to controls. Finally, in vivo administration of the histone deacetylase inhibitor trichostatin A (TSA) corrected the site-specific hypoacetylation states on H3 and H4 in MRL-lpr/lpr mice with improvement of disease phenotype. Thus, this study is the first to establish the association between aberrant histone codes and pathogenesis of autoimmune disease SLE. These aberrant post-translational histone modifications can therefore be reset with histone deacetylase inhibition in vivo.


Subject(s)
Epigenesis, Genetic , Gene Expression Regulation , Histone Deacetylase Inhibitors , Histones/chemistry , Lupus Vulgaris/genetics , Lupus Vulgaris/metabolism , Amino Acid Sequence , Animals , Autoimmune Diseases/metabolism , Chromatography, High Pressure Liquid , DNA/chemistry , DNA Methylation , Disease Models, Animal , Female , Histone Deacetylases/metabolism , Histones/metabolism , Humans , Hydroxamic Acids/pharmacology , Isotopes , Lupus Erythematosus, Systemic/metabolism , Mass Spectrometry , Mice , Mice, Inbred MRL lpr , Mice, Transgenic , Molecular Sequence Data , Peptides/chemistry , Phenotype , Protein Processing, Post-Translational , Sequence Homology, Amino Acid , Spleen/cytology
6.
Toxicology ; 216(1): 59-71, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16159691

ABSTRACT

Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium verticillioides, commonly present in corn and other cereals. Exposure to FB1 causes organ-specific diseases in various species, e.g., equine leukoencephalomalacia and porcine pulmonary edema; in mice the response is hepatotoxicity. We earlier reported that ceramide synthase inhibition by FB1, the initial biochemical effect of this mycotoxin, results in modulation of cytokine network in response to accumulated free sphingoid bases. In the current study we used NZB/NZW-F1 (NZBW) mice that have modified cytokine expression and develop lupus beginning at 5 months of age. The NZBW and C57BL/6J (CBL) mice (appropriate control) were given five daily subcutaneous injections of either saline or 2.25 mg FB1/kg/day and euthanized 24 h after the last treatment. Peripheral leukocyte counts were higher after exposure to FB1 in CBL but not in NZBW. FB1 treatment caused increases of plasma alanine aminotransferase and aspartate aminotransferase activity in CBL mice indicating hepatotoxicity; no elevation of circulating liver enzymes was recorded in NZBW mice. Hepatotoxic responses were confirmed by microscopic evaluation of apoptotic cells. The FB1-induced proliferation of cells observed in CBL strain was abolished in NZBW animals. The sphinganine accumulation in liver after FB1 was equal in both strains of mice. The NZBW strain lacked the FB1-induced increases in the expression of liver tumor necrosis factor alpha, interferon gamma, receptor interacting protein (RIP), and tumor necrosis factor alpha-related apoptosis-inducing ligand (TRAIL), observed in CBL. Results confirmed our hypothesis that initial altered sphingolipid metabolism caused by FB1 leads to perturbation of liver cytokine network and ultimate cellular injury; the mice deficient in cytokine signaling are refractory to FB1 hepatotoxicity.


Subject(s)
Cytokines/metabolism , Fumonisins/toxicity , Hepatitis/immunology , Liver/drug effects , Lupus Vulgaris/immunology , Sphingosine/analogs & derivatives , Animals , Apoptosis/drug effects , Chemical and Drug Induced Liver Injury/complications , Chemical and Drug Induced Liver Injury/immunology , Chemical and Drug Induced Liver Injury/metabolism , Cytokines/immunology , Hepatitis/complications , Hepatitis/metabolism , Hepatitis, Animal/complications , Hepatitis, Animal/immunology , Hepatitis, Animal/metabolism , Immunity, Innate/drug effects , Immunity, Innate/immunology , Liver/immunology , Liver/metabolism , Lupus Vulgaris/complications , Lupus Vulgaris/metabolism , Male , Mice , Mice, Inbred Strains , Signal Transduction/drug effects , Sphingosine/metabolism
7.
Br J Nutr ; 93(6): 835-44, 2005 Jun.
Article in English | MEDLINE | ID: mdl-16022752

ABSTRACT

Energy restriction (ER) and dietary fish oil (FO) are known to reduce the severity of glomerulonephritis and increase the lifespan of lupus-prone (NZB x NZW) F1 (B/W) mice. In the present study, mice were fed either ad libitum or energy-restricted (a 40 % lower energy intake than the diet ad libitum), semi-purified diets containing 5 % maize oil or 5 % fish oil supplementation. To estimate the renal damage associated with oxidative stress, the total amounts of reactive oxygen species (ROS), cyclooxygenase-derived ROS and levels of guanidino compounds were measured. Additionally, we assessed the putative action of ER and FO on several key antioxidant enzymes measured in the kidney post-mitochondrial fraction. Results showed that the age-related increase in creatinine level was significantly reduced by ER and FO in old mice. In contrast, arginine and guanidino acetic acid levels showed a decrease with age but were increased by ER and FO. The GSH:GSSG ratio showed a significant decrease with age, whereas ER and FO feeding prevented the decrease. The age-related decrease in antioxidant scavenging superoxide dismutase, catalase and glutathione peroxidase activities were all reversed by ER and FO. The moderately decreased glutathione reductase and glutathione-S-transferase activities with age were significantly increased by ER and FO. Furthermore, the increased total ROS and cyclooxygenase-derived ROS levels were effectively reduced by ER and FO. In conclusion, our data strongly indicate that ER and FO maintain antioxidant status and GSH:GSSG ratio, thereby protecting against renal deterioration from oxidative insults during ageing.


Subject(s)
Antioxidants/metabolism , Dietary Supplements , Energy Intake/physiology , Fish Oils/administration & dosage , Guanidine/analysis , Kidney/metabolism , Lupus Vulgaris/metabolism , Acetic Acid/analysis , Aging/physiology , Animals , Arginine/analysis , Catalase/metabolism , Creatinine/analysis , Female , Glutathione/analysis , Glutathione Disulfide/analysis , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Glutathione Transferase/metabolism , Mice , Mice, Inbred Strains , Oxidative Stress/physiology , Reactive Oxygen Species/metabolism , Superoxide Dismutase/metabolism
8.
Thromb Res ; 114(5-6): 371-82, 2004.
Article in English | MEDLINE | ID: mdl-15507267

ABSTRACT

The natural targets of anti-phospholipid antibodies (aPL) and the stimuli that induce them remain unknown. Apoptotic cells have been proposed as both potential targets and immunogens for anti-phospholipid antibodies. Demonstration of selective recognition by anti-phospholipid antibodies provides support for apoptotic cells as antigenic targets. Here, we summarize data showing that prothrombin (PT) binds to apoptotic, but not viable, cells, and that apoptotic-cell bound prothrombin provides a target for human polyclonal and murine monoclonal lupus anticoagulant (LA) antibodies. We discuss findings for two monoclonal lupus anticoagulant antibodies that have high (antibody 29J3-62) or low (antibody 29I4-24) affinity, respectively, for soluble prothrombin. Despite their very different affinities for soluble prothrombin, both monoclonal antibodies reacted similarly with prothrombin bound to phospholipid or apoptotic cells. Furthermore, both antibodies enhanced the binding of prothrombin to apoptotic cells. We propose that the recognition of apoptotic cells by these prothrombin-dependent monoclonal antibodies provides a paradigm for other anti-phospholipid autoantibodies. 29I4-24 is prototypical of phospholipid-dependent anti-phospholipid antibodies, while 29J3-62 represents a prototype for phospholipid-independent anti-phospholipid antibodies. Proteins such as prothrombin and beta2-glycoprotein I (beta2GPI) bind to apoptotic cells, thereby enhancing the recognition of apoptotic cells by anti-phospholipid antibodies. Furthermore, anti-phospholipid antibodies potentiate the interaction of these proteins with apoptotic cells. While it is unclear whether apoptotic cells are the inducing stimuli in patients with anti-phospholipid antibodies or even whether anti-phospholipid antibodies interact with apoptotic cells in vivo, it is nonetheless clear that anti-phospholipid antibodies have the potential to affect both the procoagulant activity and the uptake and clearance of apoptotic cells.


Subject(s)
Antibodies, Antiphospholipid/physiology , Apoptosis , Prothrombin/chemistry , Animals , Antibodies, Monoclonal/chemistry , Anticoagulants/chemistry , Antigens/chemistry , Binding Sites , Dose-Response Relationship, Immunologic , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Glycoproteins/chemistry , Humans , Immunoglobulin G/chemistry , Lupus Vulgaris/metabolism , Mice , Phospholipids/chemistry , Protein Binding , beta 2-Glycoprotein I
9.
J Mol Endocrinol ; 31(3): 373-99, 2003 Dec.
Article in English | MEDLINE | ID: mdl-14664701

ABSTRACT

Lymphocyte development, selection and education represent tightly controlled immune processes that normally prevent autoimmunity. Lymphocyte development likely induces cellular selection through apoptosis to remove potentially autoreactive cells. Dysregulated apoptosis, both interrupted as well as accelerated apoptosis, are now demonstrated as central defects in diverse murine autoimmune disease. In murine models of autoimmune lupus, mutations in cell death receptor Fas (CD95) and its ligand, FasL (CD95 L), have been identified. These errors create a lymphoid system resistant to apoptosis. In contrast, select lymphoid subpopulations of maturing autoimmune prone non-obese diabetic mice have identifiable and pathogenic T cells with both in vivo and in vitro heightened apoptosis after drug interventions. In part, these defects are due to faulty activation of transcription factors such as nuclear factor-kappaB (NF-kappaB) that normally protect against apoptotic death. The genetic basis of interrupted NF-kappaB in pathogenic memory T cells in diabetes is attributable to a developmentally controlled gene defect in an essential subunit of the proteasome. No specific gene in most common forms of human autoimmune disease has yet been identified. Functional assays from diverse laboratories repeatedly demonstrate heightened apoptosis in multiple cellular signaling pathways for cell death, suggesting a common theme in disease causality.


Subject(s)
Apoptosis/immunology , Autoimmunity/immunology , Lymphocytes/metabolism , Membrane Glycoproteins/genetics , fas Receptor/genetics , Animals , Apoptosis/genetics , Apoptosis/physiology , Autoimmunity/genetics , Fas Ligand Protein , Humans , Lupus Vulgaris/immunology , Lupus Vulgaris/metabolism , Lymphocytes/cytology , Major Histocompatibility Complex , Membrane Glycoproteins/metabolism , Mice , Mice, Inbred NOD , Mutation , NF-kappa B/metabolism , Proteasome Endopeptidase Complex/metabolism , T-Lymphocytes/cytology , T-Lymphocytes/metabolism , fas Receptor/metabolism
10.
FEBS Lett ; 553(3): 245-9, 2003 Oct 23.
Article in English | MEDLINE | ID: mdl-14572632

ABSTRACT

Increased expression of p202 (52 kDa), an interferon (IFN)-inducible murine protein, in splenic cells (B- and T-cells) derived from female mice of the lupus-prone strains is correlated with increased susceptibility to develop systemic lupus erythematosus. However, the molecular mechanisms remain unclear. Our previous studies have indicated that, in IFN-treated fibroblasts, p202 is detected both in the cytoplasm and in the nucleus. Moreover, in the cytoplasm, a fraction of p202 associates with a membranous organelle. Here we report that, in the cytoplasm, a fraction of p202 associated with mitochondria. Additionally, we found that the constitutive p202 is primarily detected in the cytoplasm. Remarkably, the IFN treatment of cells potentiated nuclear accumulation of p202. Our observations are consistent with the possibility that IFN signaling regulates p202 levels as well as its nucleocytoplasmic distribution. These observations will serve as a basis to elucidate the molecular mechanisms by which p202 contributes to lupus susceptibility.


Subject(s)
Carrier Proteins/metabolism , Interferon Type I/pharmacology , Intracellular Signaling Peptides and Proteins , Lupus Vulgaris/metabolism , Nuclear Proteins/metabolism , Phosphoproteins/metabolism , Animals , Carrier Proteins/genetics , Cell Nucleus/metabolism , Cytoplasm/metabolism , Disease Susceptibility , Embryo, Mammalian/cytology , Fibroblasts/cytology , Fibroblasts/metabolism , Fluorescent Antibody Technique , Green Fluorescent Proteins , Immunoblotting , Luminescent Proteins/genetics , Lupus Vulgaris/genetics , Mice , Mice, Congenic , Mitochondria/metabolism , NIH 3T3 Cells , Nuclear Proteins/genetics , Phosphoproteins/genetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Transfection
12.
Clin Exp Immunol ; 123(2): 196-202, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11207648

ABSTRACT

Complement and Fcgamma receptors are known to mediate the processing of immune complexes (IC), and abnormalities in these mechanisms may predispose to the development of lupus. We explored the processing of IC in mice deficient in complement component C1q. 125I-labelled IC comprising Hepatitis B surface antigen (HBsAg)/human anti-HBsAg (HBsAg/Ab) were injected intravenously and the sites of IC clearance determined by direct counting of organ uptake at various time points. The liver and spleen were the main sites of IC uptake in all mice. The splenic uptake of IC was significantly reduced in the C1q-deficient mice compared with the control mice. C1q-deficient mice also exhibited an initial accelerated hepatic uptake of IC similar to that seen in human subjects with hypocomplementaemia. The hepatic localization of IC at later time points was similar in both groups of mice. These data in mice are consistent with previous observations in humans that confirm that the classical pathway of complement plays an important role in the appropriate processing of IC in vivo.


Subject(s)
Antigen-Antibody Complex/metabolism , Complement C1q/metabolism , Animals , Antigen-Antibody Complex/genetics , Antigen-Antibody Complex/immunology , Complement C1q/genetics , Complement C1q/immunology , Gene Deletion , Gene Expression Regulation/immunology , Humans , Lupus Vulgaris/etiology , Lupus Vulgaris/genetics , Lupus Vulgaris/immunology , Lupus Vulgaris/metabolism , Mice
13.
Am J Pathol ; 152(4): 953-62, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9546356

ABSTRACT

Clusterin, a widely distributed glycoprotein, is detected in most tissues and in numerous physiological fluids. In the kidney, this protein is constitutively expressed in tubular epithelial cells, and its expression is enhanced following tubular injuries. In addition, clusterin has been detected in glomerular immune deposits of glomerulonephritis. The present study was designed to define the sites of clusterin mRNA accumulation in murine lupus-like nephritis in comparison with murine tubulopathies. In lupus-like nephritis, a significant increase of clusterin mRNA abundance was demonstrated. This up-regulation was localized exclusively in tubular epithelial cells exhibiting tubulointerstitial alterations, whereas no clusterin mRNA was detectable in diseased glomeruli, excluding an active synthesis of clusterin by glomerular cells. A similar tubular increase of clusterin mRNA abundance was observed in myeloma-like cast nephropathy induced by IgG3 monoclonal cryoglobulins and even in the absence of any detectable histological alterations in a model of septic shock induced by the injection of bacterial lipopolysaccharides. Our results suggest that tubular epithelial cells are the only sites of clusterin mRNA accumulation during the course of lupus-like nephritis and that the tubular up-regulation of clusterin gene expression may reflect the cellular response to various types of tubular injuries.


Subject(s)
Gene Expression Regulation , Glycoproteins/metabolism , Kidney Diseases/metabolism , Kidney Tubules/metabolism , Lupus Vulgaris/metabolism , Molecular Chaperones , Nephritis/metabolism , Animals , Clusterin , Complement Inactivator Proteins/metabolism , Female , Heymann Nephritis Antigenic Complex , Immunoglobulin G/adverse effects , In Situ Hybridization , Kidney Diseases/chemically induced , Kidney Diseases/pathology , Kidney Glomerulus/immunology , Kidney Glomerulus/metabolism , Kidney Glomerulus/pathology , Kidney Tubular Necrosis, Acute/chemically induced , Kidney Tubular Necrosis, Acute/metabolism , Kidney Tubular Necrosis, Acute/pathology , Kidney Tubules/pathology , Lipopolysaccharides/adverse effects , Lupus Vulgaris/pathology , Male , Membrane Glycoproteins/metabolism , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Inbred Strains , Nephritis/chemically induced , Nephritis/pathology , Nerve Tissue Proteins/metabolism , RNA, Messenger/analysis
14.
Am J Pathol ; 151(3): 725-34, 1997 Sep.
Article in English | MEDLINE | ID: mdl-9284821

ABSTRACT

Quantitative reverse transcription polymerase chain reaction and in situ hybridization were employed to investigate the expression of tissue-type and urokinase-type plasminogen activators (t-PA and u-PA, respectively), of their specific inhibitor (PAI-1), and of the procoagulant molecule tissue factor (TF) in tissues from mice that develop autoimmune disease (MRL lpr/lpr). A dramatic increase in PAI-1 activity in plasma and in PAI-1 mRNA in the kidneys was observed in these mice, and this increase appeared to correlate with the progression of lupus nephritis. The increase in PAI-1 mRNA was relatively specific for the kidney as little or no change was observed in most other tissues. One exception was the brain where PAI-1 mRNA was also significantly higher in the diseased mice. In addition to these changes in PAI-1, decreases in u-PA mRNA and increases in TF mRNA were demonstrated in kidneys from the lupus-prone mice. These changes also correlated with the development of lupus nephritis and with spontaneous glomerular and peritubular fibrin deposition in the nephritic kidney. In this regard, the MRL lpr/lpr mice were found to be considerably more sensitive to endotoxin than the normal controls, developing fibrin deposits in the kidneys and other tissues at 10- to 20-fold lower concentrations of this toxic agent. The increase in PAI-1 and TF mRNAs and the decrease in u-PA mRNA in the kidneys of MRL lpr/lpr mice suggests that changes in the expression of these genes may promote the formation of microthrombi and thus contribute to the progression of lupus nephritis in this model.


Subject(s)
Kidney/metabolism , Lupus Vulgaris/metabolism , Plasminogen Activator Inhibitor 1/metabolism , Thromboplastin/metabolism , Tissue Plasminogen Activator/metabolism , Urokinase-Type Plasminogen Activator/metabolism , Aging , Animals , Brain/metabolism , Female , Fibrin/metabolism , In Situ Hybridization , Kidney/drug effects , Lipopolysaccharides/pharmacology , Liver/metabolism , Lung/metabolism , Mice , Mice, Inbred Strains , Myocardium/metabolism , Plasminogen Activator Inhibitor 1/blood , Plasminogen Activator Inhibitor 1/genetics , Polymerase Chain Reaction , RNA, Messenger/metabolism , Spleen/metabolism , Thromboplastin/genetics , Tissue Distribution , Tissue Plasminogen Activator/genetics , Urokinase-Type Plasminogen Activator/genetics
15.
J Am Soc Nephrol ; 8(5): 720-9, 1997 May.
Article in English | MEDLINE | ID: mdl-9176841

ABSTRACT

Mononuclear cell infiltration in glomeruli and renal interstitium is a prominent feature of some types of glomerulonephritis, including lupus nephritis. The mechanism(s) underlying monocyte influx into the kidney is not fully understood. Recently, monocyte chemoattractant protein-1 (MCP-1) has been identified as a chemotactic factor involved in the recruitment of monocytes/macrophages in the glomeruli of rats with mesangioproliferative as well as anti-glomerular basement membrane glomerulonephritis. In the study presented here, renal MCP-1 mRNA expression in New Zealand Black x New Zealand White (NZB/W) F1 mice, a model of genetically determined immune complex disease that mimics systemic lupus in humans, was investigated. Northern blot analysis revealed a single 0.7 kb MCP-1 transcript of very low intensity in kidneys from 2-month-old NZB/W mice that had not yet developed proteinuria nor renal damage. Message levels, which increased markedly with the progression of nephritis and in association with mononuclear cell infiltration, were 10- and 15- fold higher in 8-10-month-old mice than in 2-month-old mice. By in situ hybridization, increased expression of MCP-1 mRNA was demonstrated in glomeruli and, even more striking, in tubular epithelial cells. Western blot analysis demonstrated increased expression of MCP-1 protein in kidneys of 10-month-old NZB/W mice, consistent with MCP-1 mRNA data. When NZB/W mice were treated with cyclophosphamide up to 12 months of age, expression of MCP-1 in the renal tissue remained low, the influx of inflammatory cells did not appear, and glomerular and tubular structures remained well preserved. These data suggest that elevated MCP-1 might act as a signal for inflammatory cells to infiltrate the kidney in lupus nephritis.


Subject(s)
Autoimmune Diseases/metabolism , Chemokine CCL2/metabolism , Kidney/metabolism , Lupus Vulgaris/metabolism , Animals , Autoimmune Diseases/pathology , Autoimmune Diseases/physiopathology , Chemokine CCL2/genetics , Cyclophosphamide/pharmacology , Female , Gene Expression , Immunosuppressive Agents/pharmacology , In Situ Hybridization , Kidney/pathology , Kidney/physiopathology , Lupus Vulgaris/pathology , Lupus Vulgaris/physiopathology , Mice , Mice, Inbred NZB , Proteinuria/urine , Time Factors
16.
Brain Behav Immun ; 10(4): 337-50, 1996 Dec.
Article in English | MEDLINE | ID: mdl-9045749

ABSTRACT

Immune reactions and mitogen stimulation of mammals and chickens lead to an increase of glucocorticoid (GC) plasma levels concomitant with the immune response. Interleukin (IL) 1, one of the most important glucocorticoid increasing factors produced by cells of the immune system, acts via the hypothalamo-pituitary-adrenal (HPA) axis. This pattern of immunoendocrine feedback communication is altered in autoimmune disease (AID) and represents a possible site of action for GC therapy. In the present study we investigated the role and possible underlying mechanisms of a disturbed immunoendocrine communication via the HPA axis in murine lupus. We analyzed the response to recombinant human (rhu) IL-1alpha in AID-prone mice [NZB, NZW, (NZB/NZW)F1, MRL/MP-lpr] in comparison to nonautoimmune, normal control mice (Swiss, C3H/HeJ, MRL/MP-+/+) at different levels of the HPA axis. To this end, we quantified the plasma levels of ACTH, corticosterone, and corticosterone-binding globulin (CBG) and determined various pathology parameters for autoimmunity. AID-prone mice produced nearly the same levels of plasma corticosterone after injection of rhu IL-1alpha as normal mice, but had baseline corticosterone levels consistently higher, thus resulting in significantly lower corticosterone increasing ratios. ACTH levels increased after rhu IL-1alpha injection, but there was no clearcut difference in the increasing ratios of AID-prone and normal strains. CBG levels showed no difference. As expected, there was a correlation of pathology parameters for autoimmunity and the altered immunomodulatory response to rhu IL-1alpha per group. On an individual basis, there was no such correlation. In conclusion, our results confirm the existence of a disturbed immunoendocrine communication in AID-prone mice. This disturbance clearly differs from individual to individual and also among different types of AID.


Subject(s)
Hypothalamo-Hypophyseal System/immunology , Lupus Vulgaris/immunology , Lupus Vulgaris/metabolism , Pituitary-Adrenal System/immunology , Animals , Autoradiography , Humans , Mice , Mice, Inbred C3H
18.
J Biol Chem ; 271(2): 1118-26, 1996 Jan 12.
Article in English | MEDLINE | ID: mdl-8557639

ABSTRACT

The trace interferon-alpha-induced protein, p36, was induced in Raji cells in association with lupus inclusions. It was solubilized in a nonionic detergent buffer, enriched by differential centrifugation and by preparative isoelectric focusing, and purified to homogeneity on two-dimensional protein gels. Failure to obtain N-terminal amino acid sequence, however, suggested a blocked alpha-amino group. Sequences of six tryptic peptides, 13-19 amino acids in length, were obtained after digestion, microbore-high performance liquid chromotography purification, and chemical sequence analysis. None of the six sequences, which represented approximately 25% of the entire protein, shared any meaningful homologies with entries in protein sequence repositories. Raji-cell p36 was shown in Western blots with antipeptide antibodies to be induced at least 400-fold and by immunofluorescence microscopy to co-localize with the endoplasmic reticulum resident protein, protein disulfide isomerase. These results show that p36 is a new interferon-alpha-induced protein that localizes in the endoplasmic reticulum, the cell region in which the lupus inclusions form, and that p36 is probably physically associated with the lupus inclusions.


Subject(s)
Annexin A2/isolation & purification , Cytoplasmic Granules/metabolism , Interferon-alpha/pharmacology , Lupus Vulgaris/metabolism , Amino Acid Sequence , Annexin A2/biosynthesis , Annexin A2/genetics , B-Lymphocytes/metabolism , B-Lymphocytes/pathology , B-Lymphocytes/ultrastructure , Chromatography, High Pressure Liquid , Cytoplasmic Granules/ultrastructure , Humans , Microscopy, Electron , Molecular Sequence Data , Molecular Weight , Tumor Cells, Cultured
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