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1.
J Mol Med (Berl) ; 94(3): 361-70, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26594018

ABSTRACT

The causative agent of Lyme borreliosis, Borrelia burgdorferi, is transmitted by Ixodes ticks. During tick feeding, B. burgdorferi migrates from the tick gut to the salivary glands from where transmission to the host occurs. B. burgdorferi-interacting tick proteins might serve as vaccine targets to thwart B. burgdorferi transmission. A previous screening for B. burgdorferi-interacting Ixodes scapularis gut proteins identified an I. scapularis putative dystroglycan protein (ISCW015049). Here, we describe the ISCW015049's protein structure and its cellular location in the tick gut in relation to B. burgdorferi migration. Secondly, in vivo B. burgdorferi-tick attachment murine models were performed to study the role of ISCW015049 during B. burgdorferi migration and transmission. In silico analysis confirmed that ISCW015049 is similar to dystroglycan and was named I. scapularis dystroglycan-like protein (ISDLP). Confocal microscopy of gut tissue showed that ISDLP is expressed on the surface of gut cells, is upregulated during tick feeding, and is expressed significantly higher in infected ticks compared to uninfected ticks. Inhibition of ISDLP by RNA interference (RNAi) resulted in lower B. burgdorferi transmission to mice. In conclusion, we have identified a dystroglycan-like protein in I. scapularis gut that can bind to B. burgdorferi and promotes B. burgdorferi migration from the tick gut. Key messages: B. burgdorferi exploits tick proteins to orchestrate its transmission to the host. B. burgdorferi is able bind to an I. scapularis dystroglycan-like protein (ISDLP). Inhibition of ISDLP in ticks results in lower B. burgdorferi transmission to mice. ISDLP is a potential target to prevent Lyme borreliosis.


Subject(s)
Arthropod Proteins/metabolism , Borrelia burgdorferi/physiology , Dystroglycans/metabolism , Ixodes/microbiology , Lyme Disease/prevention & control , Lyme Disease/transmission , Animals , Arthropod Proteins/genetics , Arthropod Proteins/therapeutic use , Dystroglycans/genetics , Dystroglycans/therapeutic use , Humans , Immunization , Ixodes/genetics , Ixodes/physiology , Lyme Disease/metabolism , Lyme Disease Vaccines/genetics , Lyme Disease Vaccines/metabolism , Lyme Disease Vaccines/therapeutic use , Mice , RNA, Small Interfering/genetics , RNA, Small Interfering/therapeutic use , RNAi Therapeutics , Rabbits , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Recombinant Proteins/therapeutic use
2.
Clin Vaccine Immunol ; 18(6): 901-6, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21525304

ABSTRACT

Borrelia burgdorferi OspC is an outer membrane lipoprotein required for the establishment of infection in mammals. Due to its universal distribution among B. burgdorferi sensu lato strains and high antigenicity, it is being explored for the development of a next-generation Lyme disease vaccine. An understanding of the surface presentation of OspC will facilitate efforts to maximize its potential as a vaccine candidate. OspC forms homodimers at the cell surface, and it has been hypothesized that it may also form oligomeric arrays. Here, we employ site-directed mutagenesis to test the hypothesis that interdimeric disulfide bonds at cysteine 130 (C130) mediate oligomerization. B. burgdorferi B31 ospC was replaced with a C130A substitution mutant to yield strain B31::ospC(C130A). Recombinant protein was also generated. Disulfide-bond-dependent oligomer formation was demonstrated and determined to be dependent on C130. Oligomerization was not required for in vivo function, as B31::ospC(C130A) retained infectivity and disseminated normally. The total IgG response and the induced isotype pattern were similar between mice infected with untransformed B31 and those infected with the B31::ospC(C130A) strain. These data indicate that the immune response to OspC is not significantly altered by formation of OspC oligomers, a finding that has significant implications in Lyme disease vaccine design.


Subject(s)
Antigens, Bacterial/metabolism , Bacterial Outer Membrane Proteins/metabolism , Disulfides , Lyme Disease Vaccines/metabolism , Protein Multimerization , Virulence Factors/metabolism , Amino Acid Substitution/genetics , Animals , Antibodies, Bacterial/blood , Antigens, Bacterial/chemistry , Antigens, Bacterial/genetics , Antigens, Bacterial/immunology , Bacterial Outer Membrane Proteins/chemistry , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/immunology , Borrelia burgdorferi Group/genetics , Borrelia burgdorferi Group/immunology , Cysteine/genetics , Immunoglobulin G/blood , Lyme Disease Vaccines/chemistry , Lyme Disease Vaccines/genetics , Lyme Disease Vaccines/immunology , Mice , Mice, Inbred C3H , Mutagenesis, Site-Directed , Virulence Factors/chemistry , Virulence Factors/genetics , Virulence Factors/immunology
3.
Protein Sci ; 10(10): 2083-92, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11567099

ABSTRACT

We previously demonstrated that a beta-hairpin peptide, termed BH(9-10), derived from a single-layer beta-sheet of Borrelia OspA protein, formed a native-like beta-turn in trifluoroethanol (TFE) solution, and it assembled into amyloid-like fibrils at higher TFE concentrations. This peptide is highly charged, and fibrillization of such a hydrophilic peptide is quite unusual. In this study, we designed a circularly permutated peptide of BH(9-10), termed BH(10-9). When folded into their respective beta-hairpin structures found in OspA, these peptides would have identical cross-strand interactions but different turns connecting the strands. NMR study revealed that BH(10-9) had little propensity to form a turn structure both in aqueous and TFE solutions. At higher TFE concentration, BH(10-9) precipitated with a concomitant alpha-to-beta conformational conversion, in a similar manner to the BH(9-10) fibrillization. However, the BH(10-9) precipitates were nonfibrillar aggregation. The precipitation kinetics of BH(10-9) was exponential, consistent with a first-order molecular assembly reaction, while the fibrillization of BH(9-10) showed sigmoidal kinetics, indicative of a two-step reaction consisting of nucleation and molecular assembly. The correlation between native-like turn formation and fibrillization of our peptide system strongly suggests that BH(9-10) adopts a native-like beta-hairpin conformation in the fibrils. Remarkably, seeding with the preformed BH(10-9) precipitates changed the two-step BH(9-10) fibrillization to a one-step molecular assembly reaction, and disrupted the BH(9-10) fibril structure, indicating interactions between the BH(10-9) aggregates and the BH(9-10) peptide. Our results suggest that, in these peptides, cross-strand interactions are the driving force for molecular assembly, and turn formation limits modes of peptide assembly.


Subject(s)
Antigens, Surface/chemistry , Bacterial Outer Membrane Proteins/chemistry , Bacterial Proteins/chemistry , Borrelia/chemistry , Lipoproteins , Lyme Disease Vaccines/chemistry , Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/metabolism , Bacterial Proteins/metabolism , Bacterial Vaccines , Chemical Precipitation , Kinetics , Lyme Disease Vaccines/metabolism , Models, Molecular , Peptides/chemistry , Peptides/metabolism , Protein Structure, Secondary
4.
J Immunol ; 166(12): 7398-403, 2001 Jun 15.
Article in English | MEDLINE | ID: mdl-11390491

ABSTRACT

Borrelia burgdorferi outer surface protein (Osp) A is preferentially expressed by spirochetes in the Ixodes scapularis gut and facilitates pathogen-vector adherence in vitro. Here we examined B. burgdorferi-tick interactions in vivo by using Abs directed against OspA from each of the three major B. burgdorferi sensu lato genospecies: B. burgdorferi sensu stricto, Borrelia afzelii, and Borrelia garinii. Abs directed against B. burgdorferi sensu stricto (isolate N40) destroy the spirochete and can protect mice from infection. In contrast, antisera raised against OspA from B. afzelii (isolate ACA-1) and B. garinii (isolate ZQ-1) bind to B. burgdorferi N40 but are not borreliacidal against the N40 isolate. Our present studies assess whether these selected OspA Abs interfere with B. burgdorferi-tick attachment in a murine model of Lyme disease with I. scapularis. We examined engorged ticks that had fed on B. burgdorferi N40-infected scid mice previously treated with OspA (N40, ACA-1, ZQ-1, or mAb C3.78) or control Abs. OspA-N40 antisera or mAb C3.78 destroyed B. burgdorferi N40 within the engorged ticks. In contrast, treatment of mice with OspA-ACA-1 and OspA-ZQ-1 antisera did not kill B. burgdorferi N40 within the ticks but did effectively interfere with B. burgdorferi-I. scapularis adherence, thereby preventing efficient colonization of the vector. These studies show that nonborreliacidal OspA Abs can inhibit B. burgdorferi attachment to the tick gut, highlighting the importance of OspA in spirochete-arthropod interactions in vivo.


Subject(s)
Anti-Bacterial Agents/administration & dosage , Antibodies, Bacterial/administration & dosage , Antigens, Surface/immunology , Bacterial Outer Membrane Proteins/immunology , Borrelia burgdorferi Group/immunology , Ixodes/immunology , Ixodes/microbiology , Lipoproteins , Lyme Disease Vaccines/immunology , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/metabolism , Antibodies, Bacterial/chemistry , Antibodies, Bacterial/metabolism , Antibodies, Monoclonal/administration & dosage , Antibodies, Monoclonal/chemistry , Antibodies, Monoclonal/metabolism , Antigens, Surface/genetics , Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/metabolism , Bacterial Vaccines , Binding Sites, Antibody/genetics , Borrelia burgdorferi Group/genetics , Digestive System/immunology , Digestive System/metabolism , Digestive System/microbiology , Epitopes/genetics , Epitopes/metabolism , Female , Immune Sera/administration & dosage , Immune Sera/chemistry , Immune Sera/metabolism , Injections, Intraperitoneal , Injections, Subcutaneous , Ixodes/anatomy & histology , Ixodes/metabolism , Lyme Disease/immunology , Lyme Disease/prevention & control , Lyme Disease Vaccines/genetics , Lyme Disease Vaccines/metabolism , Mice , Mice, SCID , Mutation , Protein Structure, Tertiary/genetics , Recombinant Proteins/immunology , Recombinant Proteins/metabolism
5.
J Mol Biol ; 308(2): 367-75, 2001 Apr 27.
Article in English | MEDLINE | ID: mdl-11327773

ABSTRACT

Borrelia outer surface protein A (OspA) contains a unique single-layer beta-sheet that connects N and C-terminal globular domains. This single-layer beta-sheet segment (beta-strands 8-10) is highly stable in solution, although it is exposed to the solvent on both faces of the sheet and thus it does not contain a hydrophobic core. Here, we tested whether interactions with the C-terminal domain are essential for the formation of the single-layer beta-sheet. We characterized the solution structure, dynamics and stability of an OspA fragment corresponding to beta-strands 1-12 (termed OspA[27-163]), which lacks a majority of the C-terminal globular domain. Analyses of NMR chemical shifts and backbone nuclear Overhauser effect (NOE) connectivities showed that OspA[27-163] is folded except the 12th and final beta-strand. (1)H-(15)N heteronuclear NOE measurements and amide H-(2)H exchange revealed that the single-layer beta-sheet in this fragment is more flexible than the corresponding region in full-length OspA. Thermal-denaturation experiments using differential scanning calorimetry and NMR spectroscopy revealed that the N-terminal globular domain in the fragment has a conformational stability similar to that of the same region in the full-length protein, and that the single-layer beta-sheet region also has a modest thermal stability. These results demonstrate that the unique single-layer beta-sheet retains its conformation in the absence of its interactions with the C-terminal domain. This fragment is significantly smaller than the full-length OspA, and thus it is expected to facilitate studies of the folding mechanism of this unusual beta-sheet structure.


Subject(s)
Antigens, Surface/chemistry , Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/chemistry , Bacterial Outer Membrane Proteins/metabolism , Borrelia burgdorferi Group/chemistry , Lipoproteins , Lyme Disease Vaccines/chemistry , Lyme Disease Vaccines/metabolism , Amino Acid Sequence , Bacterial Vaccines , Calorimetry, Differential Scanning , Hot Temperature , Models, Molecular , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Pliability , Protein Denaturation , Protein Structure, Secondary , Protein Structure, Tertiary , Sequence Deletion
6.
Infect Immun ; 69(6): 3536-41, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11349010

ABSTRACT

Here we describe a protocol for purifying Borrelia burgdorferi from feeding ticks by velocity centrifugation and Percoll density gradient centrifugation. The purified spirochetes were motile and 10- to 20-fold purer than the bacteria in crude tick homogenates. The purified bacteria were present in sufficient quantity for protein and gene expression studies. In comparison to culture-grown bacteria, tick-borne spirochetes had several proteins that were upregulated and a few that were downregulated. When the levels of B. burgdorferi outer surface proteins OspA and OspC were measured, OspC protein and mRNA levels were lower in cultured bacteria than in bacteria purified from ticks. Although differences in OspA mRNA levels were observed between cultured and tick-borne bacteria, no differences were observed at the protein level. These experiments demonstrate that tick-transmitted borreliae display a gene expression and antigen profile different from that of spirochetes cultured in vitro.


Subject(s)
Borrelia burgdorferi Group/isolation & purification , Ixodes/microbiology , Lipoproteins , Lyme Disease/transmission , Animals , Antigens, Bacterial/genetics , Antigens, Bacterial/immunology , Antigens, Bacterial/metabolism , Antigens, Surface/genetics , Antigens, Surface/immunology , Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/immunology , Bacterial Outer Membrane Proteins/metabolism , Bacterial Vaccines , Bacteriological Techniques , Borrelia burgdorferi Group/growth & development , Centrifugation, Density Gradient/methods , Culture Media , Feeding Behavior , Female , Ixodes/physiology , Lyme Disease/microbiology , Lyme Disease Vaccines/genetics , Lyme Disease Vaccines/immunology , Lyme Disease Vaccines/metabolism , Mice , Mice, Inbred C3H
7.
Res Microbiol ; 152(2): 149-56, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11316368

ABSTRACT

Different species of pathogenic Borrelia show different symptoms and tick vector specificity. Even within regions where only one species is found, Lyme disease progresses very differently from one patient to another. Since Borrelia shows very little recombination either within or between species, alleles of a gene can be used to mark clones. The ospC gene is highly variable within each species and can be used to define groups of related clones. It has been previously shown that only four out of seventeen ospC groups of Borrelia burgdorferi sensu stricto cause invasive forms of the disease. Other groups cause erythema migrans, a skin rash at the site of the tick bite, but not invasive disease, while still other groups seem to be nonpathogenic to humans. In this study we extend the analysis of the ospC gene to the other pathogenic species, Borrelia garinii and Borrelia afzelii. Only two groups in B. afzelii and four groups in B. garinii cause invasive disease. Thus, only ten out of the 58 defined ospC groups cause invasive and presumably chronic Lyme disease.


Subject(s)
Antigens, Bacterial , Bacterial Outer Membrane Proteins/genetics , Borrelia burgdorferi Group/genetics , Borrelia burgdorferi Group/pathogenicity , Borrelia/genetics , Genetic Variation , Lipoproteins , Animals , Antigens, Surface/genetics , Antigens, Surface/immunology , Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/immunology , Bacterial Outer Membrane Proteins/metabolism , Bacterial Vaccines , Borrelia/pathogenicity , Erythema Chronicum Migrans/microbiology , Genes, Bacterial , Humans , Lyme Disease/microbiology , Lyme Disease Vaccines/genetics , Lyme Disease Vaccines/immunology , Lyme Disease Vaccines/metabolism , Phylogeny , Ticks/microbiology , Virulence
8.
Mol Microbiol ; 40(2): 387-96, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11309121

ABSTRACT

Genetic transformation of Borrelia spp. is limited in development and has found application in only one species. For a non-genetic approach for manipulating the phenotype of these spirochaetes, we determined whether exogenous recombinant lipoproteins would incorporate in the cell's outer membrane. Using unlabelled or 125I-labelled Osp proteins, Osp-specific monoclonal antibodies, proteinase K and formaldehyde as reagents, we found that decoration of spirochaetes had the following characteristics. (i) Purified recombinant OspA or OspD lipoproteins associated with Borrelia burgdorferi and B. hermsii cells that lacked abundant lipoproteins of their own. (ii) This decoration of the cells with exogenous OspA did not affect cell's viability. (iii) The decoration was concentration and temperature dependent and stable for at least 24 h. (iv) Like native OspA, the recombinant OspA decorating the cells was accessible to antibodies and proteases and could be cross-linked to the integral outer membrane protein, P66. (v) Decoration of viable B. burgdorferi and B. hermsii with OspA rendered the cells susceptible to killing by OspA-specific antiserum. Such non-genetic alteration of the surface of a bacterium may be used to study functions and properties of lipoproteins in situ.


Subject(s)
Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/metabolism , Borrelia/classification , Borrelia/metabolism , Cell Membrane/immunology , Cell Membrane/metabolism , Lipoproteins , Lyme Disease Vaccines/metabolism , Antibodies, Bacterial/immunology , Antibodies, Monoclonal/immunology , Antigens, Surface/genetics , Bacterial Outer Membrane Proteins/genetics , Bacterial Vaccines , Borrelia/growth & development , Borrelia/immunology , Borrelia burgdorferi Group/classification , Borrelia burgdorferi Group/growth & development , Borrelia burgdorferi Group/immunology , Borrelia burgdorferi Group/metabolism , Cross-Linking Reagents , Endopeptidase K/metabolism , Formaldehyde , Lyme Disease Vaccines/genetics , Phenotype , Surface Properties
9.
J Immunol ; 166(8): 5286-91, 2001 Apr 15.
Article in English | MEDLINE | ID: mdl-11290815

ABSTRACT

Antibiotic treatment-resistant Lyme arthritis is a chronic inflammatory joint disease that follows infection with Borrelia burgdorferi (BB:). A marked Ab and T cell response to BB: outer surface protein A (OspA) often develops during prolonged episodes of arthritis. Furthermore, cross-reaction between the bacterial OspA and human LFA-1alpha(L) at the T cell level and the inability to detect BB: in the joint implicate an autoimmune mechanism. To analyze the nature of response to OspA and LFA-1alpha(L), we used OspA-specific T cell hybrids from DR4 transgenic mice, as well as cloned human cells specific for OspA(165-184), the immunodominant epitope, from five DRB1*0401(+) patients, using OspA-MHC class II tetramers. Although OspA(165-184) stimulated nearly all OspA-specific human T cell clones tested to proliferate and secrete IFN-gamma and IL-13, LFA-1alpha(L326-345) stimulated approximately 10% of these clones to proliferate and a greater percentage to secrete IL-13. Assays with LFA- or OspA-DR4 monomers revealed that higher concentrations of LFA-DR4 were needed to stimulate dual-reactive T cell hybrids. Our analysis at the clonal level demonstrates that human LFA-1alpha(L326-345) behaves as a partial agonist, perhaps playing a role in perpetuating symptoms of arthritis.


Subject(s)
Antigens, Surface/immunology , Bacterial Outer Membrane Proteins/immunology , Borrelia burgdorferi Group/immunology , Lipoproteins , Lyme Disease Vaccines/immunology , Lyme Disease/immunology , Lymphocyte Activation/immunology , Lymphocyte Function-Associated Antigen-1/immunology , Molecular Mimicry , T-Lymphocyte Subsets/immunology , Animals , Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/agonists , Bacterial Outer Membrane Proteins/metabolism , Bacterial Vaccines , Clone Cells , Humans , Hybridomas , Lyme Disease Vaccines/agonists , Lyme Disease Vaccines/metabolism , Lymphocyte Function-Associated Antigen-1/metabolism , Mice , Mice, Transgenic , Peptide Fragments/agonists , Peptide Fragments/immunology , Peptide Fragments/metabolism , T-Lymphocyte Subsets/microbiology
10.
J Clin Microbiol ; 39(3): 1169-71, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11230451

ABSTRACT

In Europe, Borrelia garinii OspA serotype 4 has been isolated from the cerebrospinal fluid of patients but, up to now, has never been identified among culture isolates from Ixodes ricinus ticks. This information raises the question of whether OspA serotype 4 is transmitted by I. ricinus in nature. In the present study, I. ricinus nymphs collected in an area of endemicity in southern Germany were allowed to feed on mice. Cultivation of ear biopsy specimens showed that six of seven B. garinii-infected mice were infected by OspA serotype 4. In contrast, very few B. garinii OspA serotype 4 organisms were isolated directly from the ticks which infected the mice; most isolates were B. afzelii. The infected mice transmitted mainly OspA serotype 4 to xenodiagnostic ticks, preferentially in combination with B. afzelii.


Subject(s)
Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/metabolism , Borrelia Infections/microbiology , Borrelia Infections/transmission , Borrelia/classification , Borrelia/physiology , Ixodes/microbiology , Lipoproteins , Lyme Disease Vaccines/metabolism , Animals , Bacterial Vaccines , Borrelia/genetics , Female , Ixodes/physiology , Mice , Mice, Inbred BALB C , Serotyping
11.
J Mol Microbiol Biotechnol ; 3(1): 83-93, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11200233

ABSTRACT

No effective recombinant vaccines are currently available for any rickettsial diseases. In this regard the first non-ribosomal DNA sequences from the obligate intracellular pathogen Piscirickettsia salmonis are presented. Genomic DNA isolated from Percoll density gradient purified P. salmonis, was used to construct an expression library in lambda ZAP II. In the absence of preexisting DNA sequence, rabbit polyclonal antiserum raised against P. salmonis, with a bias toward P. salmonis surface antigens, was used to identify immunoreactive clones. Catabolite repression of the lac promoter was required to obtain a stable clone of a 4,983 bp insert in Escherichia coli due to insert toxicity exerted by the accompanying radA open reading frame (ORF). DNA sequence analysis of the insert revealed 1 partial and 4 intact predicted ORF's. A 486 bp ORF, ospA, encoded a 17 kDa antigenic outer surface protein (OspA) with 62% amino acid sequence homology to the genus common 17 kDa outer membrane lipoprotein of Rickettsia prowazekii, previously thought confined to members of the genus Rickettsia. Palmitate incorporation demonstrated that OspA is posttranslationally lipidated in E. coli, albeit poorly expressed as a lipoprotein even after replacement of the signal sequence with the signal sequence from lpp (Braun lipoprotein) or the rickettsial 17 kDa homologue. To enhance expression, ospA was optimized for codon usage in E. coli by PCR synthesis. Expression of ospA was ultimately improved (approximately 13% of total protein) with a truncated variant lacking a signal sequence. High level expression (approximately 42% tot. prot.) was attained as an N-terminal fusion protein with the fusion product recovered as inclusion bodies in E. coli BL21. Expression of OspA in P. salmonis was confirmed by immunoblot analysis using polyclonal antibodies generated against a synthetic peptide of OspA (110-129) and a strong antibody response against OspA was detected in convalescent sera from coho salmon (Oncorhynchus kisutch).


Subject(s)
Antigens, Bacterial/genetics , Antigens, Surface/genetics , Bacterial Outer Membrane Proteins/genetics , Lipoproteins/genetics , Lyme Disease Vaccines/genetics , Proteobacteria/genetics , Amino Acid Sequence , Animals , Antigens, Bacterial/immunology , Antigens, Bacterial/metabolism , Antigens, Surface/immunology , Antigens, Surface/metabolism , Artificial Gene Fusion , Bacterial Outer Membrane Proteins/immunology , Bacterial Outer Membrane Proteins/metabolism , Bacterial Vaccines , Bacteriophage T7/genetics , Blotting, Northern/methods , Codon , Immunoblotting/methods , Intracellular Fluid/microbiology , Lipoproteins/immunology , Lipoproteins/metabolism , Lyme Disease Vaccines/immunology , Lyme Disease Vaccines/metabolism , Molecular Sequence Data , Polymerase Chain Reaction/methods , Promoter Regions, Genetic , Protein Biosynthesis , Protein Processing, Post-Translational , Protein Sorting Signals , Proteobacteria/immunology , Salmonidae/microbiology , Sequence Analysis , Transcription, Genetic
12.
J Mol Microbiol Biotechnol ; 2(4): 575-9, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11075934

ABSTRACT

We have previously demonstrated that the alphaMbeta2 integrin (known as CR3 or Mac-1) expressed on neutrophils (PMNs) and/or on CHO Mac-1 transfected cells,in the presence of serum complement binds B. burgdorferi and promotes an increased non -opsonic adhesion, in the presence of serum complement. In this study we demonstrate that: 1) living motile B. burgdorferiand recombinant lipidated OspA and OspC, up-regulate CR3 expression on PMNs; 2) in the absence of serum, B. burgdorferi induces increased adhesion of CHO cells expressing CR3 to fibronectin, an extracellular matrix protein. Both the I-domain and the lectin-like domain of CR3 are involved in the binding recognition and activation because mAb anti I-domain and N-acetyl-glucosamine inhibit cell adhesion to fibronectin. These data indicate that B. burgdorferi whole cells, but not Osps, activate CR3 integrin; since this receptor plays a key role in priming neutrophils to important inflammatory events, the interaction of B. burgdorferi with neutrophils via the CR3 may enhance their role both in defence and in disease.


Subject(s)
Antigens, Bacterial , Borrelia burgdorferi Group/physiology , Fibronectins/physiology , Macrophage-1 Antigen/genetics , Neutrophils/microbiology , Neutrophils/physiology , Animals , Antibodies, Monoclonal/pharmacology , Antigens, Surface/metabolism , Bacterial Adhesion , Bacterial Outer Membrane Proteins/metabolism , Bacterial Vaccines , Binding Sites , CHO Cells , Cell Adhesion/physiology , Cricetinae , Gene Expression Regulation , Humans , In Vitro Techniques , Lipoproteins/metabolism , Lyme Disease Vaccines/metabolism , Macrophage-1 Antigen/physiology , Recombinant Proteins/metabolism , Transfection , Up-Regulation
13.
Mol Microbiol ; 37(6): 1470-9, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10998177

ABSTRACT

The paradigm for differential antigen expression in Borrelia burgdorferi, the agent of Lyme disease, is the reciprocal expression of its outer surface (lipo)proteins (Osp) A and C; as B. burgdorferi transitions from its arthropod vector into mammalian tissue, ospC is upregulated, and ospA is downregulated. In the current study, using B. burgdorferi cultivated under varying conditions in BSK-H medium, we found that a decrease in pH, in conjunction with increases in temperature (e.g. 34 degrees C or 37 degrees C) and cell density, acted interdependently for the reciprocal expression of ospC and ospA. The lower pH (6.8), which induced the reciprocal expression of ospC and ospA in BSK-H medium, correlated with a drop in pH from 7.4 to 6.8 of tick midgut contents during tick feeding. In addition to ospC and ospA, other genes were found to be regulated in reciprocal fashion. Such genes were either ospC-like (e.g. ospF, mlp-8 and rpoS) (group I) or ospA-like (lp6.6 and p22) (group II); changes in expression occurred at the mRNA level. That the expression of rpoS, encoding a putative stress-related alternative sigma factor (sigma(s)), was ospC-like suggested that the expression of some of the group I genes may be controlled through sigma(s). The combined results prompt a model that allows for predicting the regulation of other B. burgdorferi genes that may be involved in spirochaete transmission, virulence or mammalian host immune responses.


Subject(s)
Antigens, Bacterial , Antigens, Surface/genetics , Bacterial Outer Membrane Proteins/genetics , Borrelia burgdorferi Group/genetics , Borrelia burgdorferi Group/pathogenicity , Ixodes/microbiology , Lipoproteins , Lyme Disease Vaccines/genetics , Animals , Antigens, Surface/metabolism , Bacterial Outer Membrane Proteins/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Bacterial Vaccines , Down-Regulation , Gene Expression Regulation, Bacterial , Hydrogen-Ion Concentration , Lyme Disease Vaccines/metabolism , Sigma Factor/genetics , Sigma Factor/metabolism , Species Specificity , Temperature , Transcription, Genetic , Virulence/genetics
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