ABSTRACT
Bovine leukosis virus (BLV) was transmitted to sheep in a simulated mechanical transmission experiment, using the following species of mosquitoes; Anopheles freeborni, A stephensi, A quadrimaculatus, and A albimanus. Mosquitoes were fed on blood taken from a BLV-infected cow with persistent lymphocytosis. Mouthparts and heads of mosquitoes were removed immediately after feeding, placed in RPMI 1640 medium, and inoculated subcutaneously into sheep. Nine sheep were inoculated with mouthparts and heads from 37 to 122 mosquitoes. Infection was determined serologically. Three monthly serum samples were collected from the sheep and were tested for the presence of antibodies to BLV, using the agar-gel immunodiffusion (AGID) test. Sera that were negative by AGID at 3 months were tested by radioimmunoassay. Results from radioimmunoassay agreed with those obtained by AGID. Four of the 9 sheep developed antibody to BLV. Sheep that seroconverted were inoculated with mouthparts and heads from as few as 54 mosquitoes.
