Prospecção de biomarcadores inflamatórios de síndrome coronariana aguda - resultados preliminares / Prospecting of inflamatory biomarkers in acute coronary syndrome - preliminary results

Fundamentos: Numerosas substâncias têm sido exploradas como preditores de risco em doença arterial coronariana (DARC), mas a literatura ainda carece de indicadores de síndrome coronariana aguda (SCA).Objetivos: Este estudo piloto visa a descrever níveis circulantes do fator de inibição da migração de macrófagos (MIF), da fração solúvel de ligante de CD40 (sCD40L) e da interleucina 6 (IL-6) numa amostra de pacientes sob suspeita de SCA, no sentido de explorar seu potencial como ferramentas diagnósticas.Métodos: Num período de 6 meses, uma série de pacientes atendidos em unidade de dor torácica, com e sem critérios diagnósticos de SCA, tiveram amonstras de sangue venoso periféricos coletadas com o propósito de medir e comparar a produção das citocinas nos dois grupos...

Solid-phase synthesis, isolation and analysis of a mouse protein, the macrophage migration inhibitory factor.

Murine macrophage migration inhibitory factor (mMIF) is an Mr 12,500 protein composed of all natural amino acid residues. Using the fluorenylmethoxycarbonyl chemistry for solid-phase peptide synthesis (SPPS) under special conditions, a stepwise approach was very successful leading to a crude product in unexpected high purity. After RP-HPLC isolation, a little mass difference of -12 u was determined for the received protein by matrix-assisted laser desorption ionization MS and ion spray MS and the failure sequence [Ser15]-mMIF identified by Edman degradation. The total solid-phase synthesis was repeated in the stepwise manner under the same conditions leading to the expected mMIF protein in high purity, which was confirmed by different analytical methods. Our results shows the reproducibility of our SPPS approaches to proteins and point out the importance of high-resolution mass spectrometry for a rapid and accurate analysis of such biopolymers.