ABSTRACT
Mycobacterium smegmatis cells produce [3H]methanol when incubated with [methyl-3H]methionine. The methanol is derived from S-adenosylmethionine rather than methyltetrahydrofolate. M. smegmatis cells carboxymethylate several proteins, and some of the methanol probably results from their demethylation, but most of the methanol may come from an unidentified component with a high gel mobility. Although methanol in the medium reached 19 microM, it was not incorporated into the methylated mannose polysaccharide, a lipid carrier in this organism.
Subject(s)
Methanol/biosynthesis , Mycobacterium/metabolism , Mannosides/metabolism , Methionine/metabolism , Polysaccharides/metabolism , S-AdenosylmethionineSubject(s)
Methanol/analysis , Microsomes, Liver/metabolism , Animals , Antineoplastic Agents/pharmacology , Carcinogens/pharmacology , Cell Survival , Cells, Cultured , Gas Chromatography-Mass Spectrometry/methods , Leukemia P388/metabolism , Male , Methanol/biosynthesis , Mice , Phenobarbital/pharmacology , Rats , Rats, Inbred F344ABSTRACT
Chemotactically wild type Escherichia coli were incubated with L-[methyl-3H]methionine to label the methyl groups of their methyl-accepting chemotaxis proteins. Cells were then treated to specifically demethylate these proteins. We have identified the end product of this demethylation as [3H]methanol in the cell-free medium from treated cells.
Subject(s)
Bacterial Proteins/metabolism , Chemotaxis , Escherichia coli/metabolism , Membrane Proteins/metabolism , Methanol/biosynthesis , Cell-Free System , Cold Temperature , Dealkylation , Methionine/metabolism , Methylation , Species SpecificitySubject(s)
Benz(a)Anthracenes/metabolism , Ethers, Cyclic/biosynthesis , Microsomes, Liver/metabolism , Adrenal Gland Diseases/chemically induced , Animals , Chromatography, Gel , Chromatography, Thin Layer , Crystallization , Glutathione/biosynthesis , Guanine Nucleotides/metabolism , Hydrolases/metabolism , Male , Methanol/biosynthesis , Methylcholanthrene/pharmacology , Microsomes, Liver/drug effects , Microsomes, Liver/enzymology , NADH, NADPH Oxidoreductases/metabolism , Necrosis/chemically induced , Phenols/metabolism , Rats , TritiumSubject(s)
Anti-Bacterial Agents/isolation & purification , Cyclopentanes/isolation & purification , Streptomyces/analysis , Anti-Bacterial Agents/biosynthesis , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Chemical Phenomena , Chemistry , Cyclopentanes/biosynthesis , Cyclopentanes/pharmacology , Magnetic Resonance Spectroscopy , Methanol/biosynthesis , Methanol/isolation & purification , Methanol/pharmacology , Spectrophotometry, Infrared , Streptomyces/classification , Streptomyces/metabolismSubject(s)
Adenosine/metabolism , Methanol/biosynthesis , Methionine/metabolism , Adrenal Glands/enzymology , Animals , Benzoates , Brain/enzymology , Carbon Isotopes , Chromatography, Paper , In Vitro Techniques , Kidney/enzymology , Liver/enzymology , Lung/enzymology , Methyltransferases/metabolism , Myocardium/enzymology , Pancreas/enzymology , Rats , S-Adenosylmethionine/biosynthesis , S-Adenosylmethionine/metabolism , Spleen/enzymology , Sulfur Isotopes , TritiumSubject(s)
Ethanol/biosynthesis , Intestinal Mucosa/metabolism , Adult , Animals , Chromatography, Gas , Fasting , Female , Humans , Male , Mass Spectrometry , Methanol/biosynthesis , Portal Vein , RatsSubject(s)
Erythrocytes/enzymology , Methyltransferases/blood , Animals , Carbon Isotopes , Catecholamines , Cats , Dogs , Guinea Pigs , Histamine , Humans , Methanol/biosynthesis , Rats , Species SpecificityABSTRACT
1. Experimental conditions have been found in which small amounts of methanol (approximately 2.5mm) accumulated when washed cell suspensions of methane-grown Pseudomonas methanica and Methanomonas methanooxidans were incubated with methane+oxygen mixtures in Warburg flasks. 2. The methanol formed could be separated completely from water by fractional distillation through glass helices followed by gas chromatography using 20% polyethylene glycol 400 on a Celite 545 support. 3. By using (18)O-enriched oxygen gas the abundance of (18)O in the methanol formed from oxidation of methane was measured with a Perkin-Elmer 270 combined gas chromatograph/mass spectrometer. The results showed that the oxygen in methanol was derived exclusively from gaseous oxygen in both micro-organisms. 4. Control experiments using [(18)O]water in incubation mixtures confirmed that there was negligible incorporation of the oxygen atom from water into methanol.
