Lipids of the oligodendroglial fraction in methylnitrosourea induced encephalopathy.

The investigations were performed on oligodendroglial cells isolated from the cerebral white matter of Wistar rats treated intravenously with a 3% solution of MNU at a dose of 60 mg/kg body weight. The oligodendroglial fraction showed a decrease of the sphingomyelin, phosphatidylserine and phosphoinositides content. The observed changes correlated with alterations of the lipid spectrum found in the myelin fraction of MNU intoxicated rats. This would indicate that MNU acting in the central nervous system affects in a similar way the lipid metabolism of both components of the oligodendroglia-myelin system.

[Ability of cells in tissue culture to recover from damage caused by chemotherapeutic drugs]. / Sposobnost' kletok v kul'ture tkani vosstanavlivat'sia ot povrezhdenii, indutsirovannykh nekotorymi khimioterapevticheskimi preparatami.

The repair of potential lethal damages was investigated in the LL cell line induced by some chemicals. Three different chemical compounds were used. An inhibitor of DNA synthesis--hydroxyurea, an alkylating agent--methylnitrosourea, and an antibiotic intercalating in DNA--actinomycin D. It was shown that damages induced by hydroxyurea could be repaired. Unlike, the repair of damages induced by methylnitrosourea and actinomycin D was not observed.