Subject(s)
Humans , Female , Child , Dermoscopy , Molluscum Contagiosum/diagnostic imaging , Foot Dermatoses/diagnostic imagingABSTRACT
No disponible
Subject(s)
Humans , Eyelid Diseases/diagnostic imaging , Eyelid Diseases/virology , Molluscum Contagiosum/diagnostic imaging , Tomography, Optical CoherenceABSTRACT
Molluscum contagiosum is a common, contagious viral skin disease that often affects children and adolescents. Involvement of the areola and nipple are rarely reported. Herein we report two young women with molluscum contagiosum on the areola-nipple complex and we discuss the dermoscopic features of the lesions at this unusual site.
Subject(s)
Dermoscopy , Molluscum Contagiosum/diagnostic imaging , Nipples , Adult , Female , Humans , Molluscum Contagiosum/pathology , Young AdultABSTRACT
Molluscum contagiosum virus (MCV) is a common skin pathogen of children and young adults. Infection with MCV causes benign skin tumors in children and young adults and is mostly self-limiting. In contrast to orthopoxviruses, MCV infections tend to take a subacute clinical course but may persist for up to 12 months. Current numbers for MCV seroprevalence in different geographical areas are based on a variety of historical serological methods from complement fixation assays to MCV ELISAs based on purified MCV virions and MC133 antigen expressed in a Semliki Forest Virus expression system. A standardized ELISA for the assessment of MCV seroprevalence would be useful to determine global MCV seroprevalence. The methods described show that polypeptides derived from MCV open reading frames MC084 (residues V123 to R230 and V33 to G117), mc133 (residues M1 to N370), and glutathione S-transferase (GST)-H3L (residues I142 to W251) expressed in E. coli RIL+ as GST fusion proteins can be used to assess antibody binding in a GST capture ELISA. We show how the ELISA can be used to screen a panel of patient sera previously characterized with the mc084 V123-R230 ELISA. © 2017 by John Wiley & Sons, Inc.
Subject(s)
Antibodies, Viral/blood , Enzyme-Linked Immunosorbent Assay/methods , Molluscum Contagiosum/diagnostic imaging , Molluscum contagiosum virus/immunology , Antigens, Viral/genetics , Antigens, Viral/immunology , Humans , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Seroepidemiologic StudiesABSTRACT
Handheld reflectance confocal microscopy may represent an adjunctive, fast, non-invasive tool for the diagnosis of molluscum contagiosum, revealing microscopic details closely related to histopathology, as demonstrated by this study evaluating 19 molluscum lesions in 11 patients. It permits the rapid examination of one or multiple skin lesions in real time and it is perfectly suitable for children.
Subject(s)
Dermoscopy , Molluscum Contagiosum/diagnostic imaging , Molluscum Contagiosum/pathology , Adolescent , Adult , Child , Female , Humans , Male , Microscopy, Confocal/methods , Young AdultABSTRACT
Immunohistochemical studies of one typical and two atypical cases of molluscum contagiosum with anti-CD34 monoclonal antibodies showed a tightly enclosing fine vasculature around the lesional masses of the disease. The thin interstitial septa between the lobules of the molluscum lesions also contained abundant endothelium. An electron microscopic study of a pinched-off lesion of common molluscum contagiosum demonstrated that the tightly enclosing blood vessels lacked muscle layers, suggesting that they were capillaries, being a distance of several hundred nanometers from the basal cells of the molluscum mass. A 3D constructed image of the vasculature confirmed a network of the vessels. These tightly enclosing vascular networks around the lesions of molluscum contagiosum support the rapid growth of this disease.
