ABSTRACT
A protein antigenically similar to Tamm-Horsfall glycoprotein ( THLG ) was determined in CSF by affinity chromatography followed by a radioimmunological assay. THLG seems not to be a constituent of normal CSF or an autoantigen. It probably originates from nervous tissue and not from plasma proteins. Its mean is higher in the CSF of multiple sclerosis patients than in the CSF of degenerative disorders patients. THLG seems to be a chemical component of neuronal membrane and its physiological functions remain unknown.
Subject(s)
Demyelinating Diseases/cerebrospinal fluid , Mucoproteins/cerebrospinal fluid , Blood-Brain Barrier , Central Nervous System Diseases/pathology , Demyelinating Diseases/immunology , Female , Humans , Leukocytes/pathology , Male , Mucoproteins/blood , Multiple Sclerosis/cerebrospinal fluid , Multiple Sclerosis/immunology , Necrosis/metabolism , UromodulinABSTRACT
Affinity chromatography purification was combined with a radioimmunoassay for 'Tamm-Horsfall-like' glycoprotein. This enabled serum concentrations to be established and to demonstrate its presence in cerebrospinal fluid for the first time. This assay method used in different circumstances suggests a multifocal synthesis. Nevertheless, urinary Tamm-Horsfall glycoprotein so far must be distinguished from the serum or cerebrospinal fluid Tamm-Horsfall-like glycoprotein.
