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1.
Carbohydr Polym ; 338: 122218, 2024 Aug 15.
Article in English | MEDLINE | ID: mdl-38763705

ABSTRACT

Here, biogenic and multifunctional active food coatings and packaging with UV shielding and antimicrobial properties were structured from the aqueous dispersion of an industrial byproduct, suberin, which was stabilized with amphiphilic cellulose nanofibers (CNF). The dual-functioning CNF, synthesized in a deep eutectic solvent, functioned as an efficient suberin dispersant and reinforcing agent in the packaging design. The nanofibrillar percolation network of CNF provided a steric hindrance against the coalescence of the suberin particles. The low CNF dosage of 0.5 wt% resulted in dispersion with optimal viscosity (208.70 Pa.s), enhanced stability (instability index of <0.001), and reduced particle size (9.37 ± 2.43 µm). The dispersion of suberin and CNF was further converted into self-standing films with superior UV-blocking capability, good thermal stability, improved hydrophobicity (increase in water contact angle from 61° ± 0.15 to 83° ± 5.11), and antimicrobial properties against gram-negative bacteria. Finally, the synergistic bicomponent dispersions were demonstrated as fruit coatings for bananas and packaging for strawberries to promote their self-life. The coatings and packaging considerably mitigated fruit deterioration and improved their freshness by preventing moisture loss and microbial attack. This sustainable approach is expected to pave the way toward advanced, biogenic, and active food packaging based on widely available bioresources.


Subject(s)
Cellulose , Food Packaging , Lipids , Nanofibers , Wood , Nanofibers/chemistry , Cellulose/chemistry , Food Packaging/methods , Wood/chemistry , Lipids/chemistry , Hydrophobic and Hydrophilic Interactions , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Viscosity , Musa/chemistry , Water/chemistry , Gram-Negative Bacteria/drug effects , Fruit/chemistry
3.
Food Chem ; 451: 139506, 2024 Sep 01.
Article in English | MEDLINE | ID: mdl-38703733

ABSTRACT

This study aimed to characterize and evaluate the in vitro bioactive properties of green banana pulp (GBPF), peel (GBPeF), and mixed pulp/peel flours M1 (90/10) and M2 (80/20). Lipid concentration was higher in GBPeF (7.53%), as were the levels of free and bound phenolics (577 and 653.1 mg GAE/100 g, respectively), whereas the resistant starch content was higher in GBPF (44.11%). Incorporating up to 20% GBPeF into the mixed flour had a minor effect on the starch pasting properties of GBPF. GBPeF featured rutin and trans-ferulic acid as the predominant free and bound phenolic compounds, respectively. GBPF presented different major free phenolics, though it had similar bound phenolics to GBPeF. Both M1 and M2 demonstrated a reduction in intracellular reactive oxygen species (ROS) generation. Consequently, this study validates the potential of green banana mixed flour, containing up to 20% GBPeF, for developing healthy foods and reducing post-harvest losses.


Subject(s)
Flour , Fruit , Musa , Nutritive Value , Phenols , Musa/chemistry , Flour/analysis , Fruit/chemistry , Phenols/analysis , Phenols/chemistry , Plant Extracts/chemistry , Plant Extracts/analysis , Reactive Oxygen Species/metabolism , Starch/chemistry , Starch/analysis
4.
Int J Biol Macromol ; 270(Pt 1): 132070, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38705313

ABSTRACT

Plastic pots used in horticultural nurseries generate substantial waste, causing environmental pollution. This study aimed to develop biodegradable composites from banana pseudo-stem reinforced with agricultural residues like pineapple leaves, taro and water hyacinth as eco-friendly substitutes. The aim of this study is to develop optimised banana biocomposite formulations with suitable reinforcements that balance mechanical durability, biodegradation, and seedling growth promotion properties to serve as viable eco-friendly alternatives to plastic seedling pots. This study was carried out by fabricating banana fibre mats through pulping, drying and hot pressing. Composite sheets were reinforced with 50 % pineapple, taro or water hyacinth fibres. The mechanical properties (tensile, yield strength, elongation, bursting strength), hydrophilicity (contact angle, water absorption), biodegradability (soil burial test), and seedling growth promotion were evaluated through appropriate testing methods. The results show that banana-taro composites exhibited suitable tensile strength (25 MPa), elongation (27 %), water uptake (41 %) and 82 % biodegradation in 60 days. It was observed that biodegradable seedling trays fabricated from banana-taro composite showed 95 % tomato seed germination and a 125 cm plant height increase in 30 days, superior to plastic trays. The finding shows that the study demonstrates the potential of banana-taro biocomposites as alternatives to plastic nursery pots, enabling healthy seedling growth while eliminating plastic waste pollution through biodegradation.


Subject(s)
Musa , Seedlings , Musa/growth & development , Musa/chemistry , Seedlings/growth & development , Tensile Strength , Biodegradation, Environmental , Germination , Water/chemistry
5.
Int J Biol Macromol ; 270(Pt 1): 132276, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38734352

ABSTRACT

The reuse and development of natural waste resources is a hotspots and challenges in the research of new fiber materials and the resolution of environmental concern globally. Herein, this study aimed to develop a simple and direct manual extraction process to extract Musa core fibers (MCFs) for rapid water conduction and evaporation. Through simple processes such as ring cutting and stretching, this green and non-destructive inside-out extraction strategy enabled Musa fibers to be naturally and harmlessly degummed from natural Musa stems, with good maintenance of the fiber structure and highly helical morphology. The extracted fibers are composed of regularly and closely arranged cellulose nanofibrils in the shape of ribbon spirally arranged multi-filaments, and the single filament is about 2.65 µm. The high-purity fibers exhibit ultra-high tensile strength under a non-destructive extraction process, and the ultimate tensile strength in dry state is as high as 742.95 MPa. The tensile strength is affected by the number of fiber bundles, which shows that tensile strength and tensile modulus is higher than those of vascular bundle fibers in dry or wet condition. In addition, the MCFs membrane indicates good water conductivity, with a water absorption height of 50 mm for the sample in only 60 s. Moreover, the water evaporation rate of MCFs reaches 1.37 kg m-2 h-1 in 30 min, which shows that MCFs have excellent water conductivity and evaporation rate compared with ordinary cotton fibers. These results indicate that MCFs have great potential in replacing the use of chemical methods to extract fibers from vascular bundles, providing an effective way to achieve sustainability in quick-drying applications, as well as in the sustainable development of natural waste resources.


Subject(s)
Musa , Tensile Strength , Water , Water/chemistry , Musa/chemistry , Cellulose/chemistry , Nanofibers/chemistry
6.
Int J Biol Macromol ; 270(Pt 1): 132287, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38735601

ABSTRACT

Damage to the integrity of the preservation coating on the fruit surface will seriously affect the shelf life of the fruit. In this work, the strong hydrogen bond interaction between xanthan gum (XG) and konjac glucomannan (KGM) could form hydrogel films with self-healing properties. The introduction of gallic acid (GA) was beneficial to further improve the antioxidant activity and UV shielding performance of the composite films. Surprisingly, the mechanical properties and gas (water vapor, O2 and CO2) barrier properties of the KGM film crosslinked by XG were significantly improved. The experiment of banana preservation showed that the composite coating could effectively delay the water loss and browning of bananas, slow down the decomposition of pectin and starch in the flesh, and extend the shelf life of bananas for >6 days. Therefore, this multifunctional coating is an excellent packaging material and has a very broad application prospect in the field of food preservation.


Subject(s)
Food Preservation , Mannans , Musa , Polysaccharides, Bacterial , Mannans/chemistry , Polysaccharides, Bacterial/chemistry , Musa/chemistry , Food Preservation/methods , Antioxidants/chemistry , Food Packaging/methods , Hydrogels/chemistry
7.
Food Chem ; 448: 139176, 2024 Aug 01.
Article in English | MEDLINE | ID: mdl-38574719

ABSTRACT

Using 3D printing technology, a gelatin-polyvinyl alcohol­carbon dots (GPC) layer+corn starch-polyvinyl alcohol-cinnamon essential oil (CPC) layer active bilayer film with an external barrier function and an internal controlled-release effect was successfully produced for food preservation. The GPC film was provided with potent antioxidant and UV blocking properties by the banana peel carbon dots (CDs). The cinnamon essential oil (CEO) had the strongest interaction with the film matrix at 3% (w/w), causing the CPC film having the lowest surface wettability, good integrity, and lowest crystallinity. The CEO's stability and releasing effectiveness were greatly enhanced by the creation of a bilayer film. At 60% filling rate of the CPC layer, the bilayer film showed the highest CEO retention after drying and the best CEO release performance. Finally, the created active bilayer film was found to significantly improve the sensory quality stability of the spicy essential oil microcapsule powders. It also successfully extended the mangoes' shelf life by delaying browning and rot.


Subject(s)
Cinnamomum zeylanicum , Food Packaging , Gelatin , Musa , Oils, Volatile , Printing, Three-Dimensional , Starch , Oils, Volatile/chemistry , Food Packaging/instrumentation , Cinnamomum zeylanicum/chemistry , Gelatin/chemistry , Starch/chemistry , Musa/chemistry , Carbon/chemistry , Food Preservation/instrumentation , Food Preservation/methods , Quantum Dots/chemistry , Zea mays/chemistry
8.
Int J Biol Macromol ; 267(Pt 1): 131135, 2024 May.
Article in English | MEDLINE | ID: mdl-38574914

ABSTRACT

The study involves the preparation and characterization of crosslinked-carboxymethyl cellulose (CMC) films using varying amounts of citric acid (CA) within the range 5 %-20 %, w/w, relative to the dry weight of CMC. Through techniques such as Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, carbonyl content analysis, and gel fraction measurements, the successful crosslinking between CMC and CA is confirmed. The investigation includes an analysis of chemical structure, physical and optical characteristics, swelling behavior, water vapor transmission rate, moisture content, and surface morphologies. The water resistance of the cross-linked CMC films exhibited a significant improvement when compared to the non-crosslinked CMC film. The findings indicated that films crosslinked with 10 % CA demonstrated favorable properties for application as edible coatings. These transparent films, ideal for packaging, prove effective in preserving the quality and sensory attributes of fresh bananas, including color retention, minimized weight loss, slowed ripening through inhibiting amyloplast degradation, and enhanced firmness during storage.


Subject(s)
Carboxymethylcellulose Sodium , Citric Acid , Edible Films , Food Packaging , Musa , Carboxymethylcellulose Sodium/chemistry , Citric Acid/chemistry , Food Packaging/methods , Musa/chemistry , Steam , Cross-Linking Reagents/chemistry , Spectroscopy, Fourier Transform Infrared , Water/chemistry , Food Preservation/methods
9.
J Food Sci ; 89(5): 2774-2786, 2024 May.
Article in English | MEDLINE | ID: mdl-38602038

ABSTRACT

Banana is one of the most consumed and popular fruits in all regions of the world, being cultivated mainly in tropical countries. It is not only a rich source of vitamins A, C, and B, calcium, iron, potassium, phosphorus, and other vitamins and nutrients, but it also contains several types of antioxidants with high nutritional value. In this context, the current study aimed to quantify the content of ascorbic acid, flavonoids, pigments, and minerals present in "Nanicão" bananas during the ripening process. As demonstrated, the level of flavonoids was higher in ripe and overripe fruits, whereas the mineral composition was high only at ripening stage 4 (more yellow than green) a stage that should be prioritized when recommending fruit consumption to the population deficient in these minerals. Regarding pigments, there was a reduction in chlorophylls a and b and an increase in carotenoids and anthocyanins in peels and pulps. PRACTICAL APPLICATION: Flavonoids are phenolic, bioactive compounds with proven antioxidant and anti-inflammatory activity and products of the plant's secondary metabolism. The degradation of chlorophylls and synthesis of carotenoids and anthocyanins, and as a consequence of the latter pigment, the increase in flavonoids in the pulp was evident during the monitoring of ripening, mainly in the fruit peels in relation to pigments. Minerals are essential elements, the main ones provided in balanced diets and important for dietary and nutritional health.


Subject(s)
Antioxidants , Carotenoids , Flavonoids , Fruit , Minerals , Musa , Flavonoids/analysis , Fruit/chemistry , Musa/chemistry , Minerals/analysis , Antioxidants/analysis , Carotenoids/analysis , Pigments, Biological/analysis , Chlorophyll/analysis , Ascorbic Acid/analysis , Nutritive Value , Anthocyanins/analysis
10.
Trop Anim Health Prod ; 56(4): 139, 2024 Apr 24.
Article in English | MEDLINE | ID: mdl-38656618

ABSTRACT

We aimed to evaluate the effects of different drying methods for banana residues on the energy metabolism and respirometry of growing lambs. Twenty Santa Inês x Dorper lambs were fed five experimental diets: Tifton 85 grass hay (Control), shade-dried banana leaf hay (LH Shade), shade-dried pseudostem banana hay (PH Shade), sun-dried banana leaf hay (LH Sun), and sun-dried banana pseudostem hay (PH Sun). Nutrient intake and digestibility were assessed in metabolic cages, whereas O2 consumption and CO2, methane, and heat production were measured in a respirometry chamber with animals fed at maintenance and ad libitum levels. Nutrient and energy intake was not influenced by diet. Pseudostem hay had higher apparent digestibility of dry matter (71.5%), organic matter (72.4%), and neutral detergent fiber (58.0%). However, this led to greater energy loss in the form of methane (12.1%). The banana residue hays and drying methods did not alter oxygen consumption, CO2 production, or heat production of animals fed ad libitum or during maintenance. On the other hand, the use of leaf hay resulted in a reduction of 24.7% in enteric methane production of animals fed ad libitum. The inclusion of pseudostem hay is recommended in sheep feedlot diet. This residue provided greater use of DM, however promoted a greater loss of energy in the form of methane, resulting in similar energy consumption. The drying methods did not reduce the availability of nutrients and the sun drying method is recommended, since it is a faster drying method.


Subject(s)
Animal Feed , Diet , Digestion , Energy Metabolism , Musa , Animals , Musa/chemistry , Animal Feed/analysis , Diet/veterinary , Male , Sheep, Domestic/physiology , Animal Nutritional Physiological Phenomena , Oxygen Consumption , Desiccation
11.
Braz J Microbiol ; 55(2): 1117-1129, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38647869

ABSTRACT

Global food production faces challenges concerning access to nutritious and sustainably produced food. Pleurotus djamor, however, is an edible mushroom that can be cultivated on agricultural waste. Considering that nutritional and functional potential of mushrooms can change based on cultivation conditions, we examined the influence of substrates with different compositions of banana leaf and sugarcane bagasse on the nutritional, mycochemical, and antioxidant properties of P. djamor. The mushrooms were grown for 120 days and dried in a circulating air oven at 45 °C for three days. We conducted bromatological analyses and mycochemical characterization (1H-NMR, total phenolics, and flavonoids) of the mushrooms and assayed the antioxidant activity of extracts from the dried mushrooms using an ethanol/water solution (70:30 v/v). In general, the substrates produced mushrooms with high protein (18.77 ± 0.24% to 17.80 ± 0.34%) and dietary fiber content (18.02 ± 0.05% to 19.32 ± 0.39%), and with low lipid (0.28 + 0.08% to 0.4 + 0.6%), and caloric content (maximum value: 258.42 + 8.49), with no significant differences between the groups (p ≥ 0.05). The mushrooms also exhibited high levels of total phenolics and flavonoids. The mushrooms cultivated on sugarcane bagasse substrates presented the highest values (p < 0.05). Analysis of the 1H-NMR spectra indicates an abundant presence of heteropolysaccharides, ß-glucans, α-glucans, and oligosaccharides, and all the mushroom extracts exhibited high antioxidant activity. In conclusion, our study demonstrates that agricultural residues permit sustainable production of edible mushrooms while maintaining nutritional and functional properties.


Subject(s)
Antioxidants , Cellulose , Musa , Plant Leaves , Pleurotus , Saccharum , Pleurotus/metabolism , Pleurotus/chemistry , Pleurotus/growth & development , Saccharum/chemistry , Saccharum/metabolism , Antioxidants/metabolism , Antioxidants/chemistry , Musa/chemistry , Plant Leaves/chemistry , Cellulose/metabolism , Nutritive Value , Flavonoids/metabolism , Flavonoids/analysis , Phenols/metabolism , Agriculture/methods
12.
J Evid Based Integr Med ; 29: 2515690X241249534, 2024.
Article in English | MEDLINE | ID: mdl-38679583

ABSTRACT

OBJECTIVE: Aqueous extract of unripe Musa paradisiaca fruit is commonly used for the treatment of ulcers in eastern Nigeria. This study aimed to assess the acute and subacute effects of an aqueous extract of unripe fruit on male and female fertility in rats. METHODS: Aqueous extracts obtained by maceration were analyzed for acute and subacute toxicity and for the presence of phytochemical constituents using standard procedures. The extract (100, 500, and 1000 mg/kg) was administered daily to rats of both sexes for 28 d. Blood samples collected on days 0 and 28 were assessed for follicle-stimulating hormone (FSH), luteinizing hormone (LH), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA). Testes and ovaries were harvested for histopathological analysis. Sperm were also collected to determine the sperm count and motility. RESULTS: Phytochemical screening revealed the presence of saponins, tannins, alkaloids, and resins. After an oral dose of up to 5000 mg/kg, there were no deaths in the acute toxicity test. The extract (500 mg/kg) significantly (P < .05) enhanced sperm count and motility relative to the untreated control; significantly (P < .05) reduced SOD, CAT, and glutathione levels, while significantly (P < .05) elevated LH, FSH, and MDA levels in male and female rats. Histological examination revealed significant structural damage to the ovaries. CONCLUSION: Unripe Musa paradisiaca fruit exhibited an adverse toxicological profile following prolonged administration and caused oxidative stress in rodents.


Subject(s)
Follicle Stimulating Hormone , Luteinizing Hormone , Musa , Plant Extracts , Animals , Male , Female , Plant Extracts/pharmacology , Rats , Musa/chemistry , Luteinizing Hormone/blood , Follicle Stimulating Hormone/blood , Malondialdehyde/metabolism , Superoxide Dismutase/metabolism , Reproduction/drug effects , Ovary/drug effects , Nigeria , Catalase/metabolism , Testis/drug effects , Sperm Count , Fruit , Sperm Motility/drug effects , Rats, Wistar
13.
J Sci Food Agric ; 104(8): 4485-4497, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38483269

ABSTRACT

Crop breeding in sub-Saharan Africa has made considerable gains; however, postharvest and food-related preferences have been overlooked, in addition to how these preferences vary by gender, social difference and context. This context is changing as participatory approaches using intersectional gender and place-based methods are beginning to inform how breeding programmes make decisions. This article presents an innovative methodology to inclusively and democratically prioritise food quality traits of root, tuber and banana crops based on engagement with food systems actors and transdisciplinary collaboration. The outcome of the methodology is the Gendered Food Product Profile (GFPP) - a list of prioritised food quality characteristics - to support breeders to make more socially inclusive decisions on the methods for trait characterisation to select genotypes closer to the needs of food system actors. This article reviews application of the methodology in 14 GFPPs, presents illustrative case studies and lessons learned. Key lessons are that the transdisciplinary structure and the key role of social scientists helped avoid reductionism, supported co-learning, and the creation of GFPPs that represented the diverse interests of food system actors, particularly women, in situ. The method partially addressed power dynamics in multidisciplinary decision making; however, effectiveness was dependent on equitable team relations and supportive institutions committed to valuing plural forms of knowledge. Actions to address power asymmetries that privilege particular types of knowledge and voices in decision making are crucial in techno-science projects, along with opportunities for co-learning and long-term collaboration and a transdisciplinary structure at higher level. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.


Subject(s)
Crops, Agricultural , Decision Making , Humans , Female , Male , Crops, Agricultural/growth & development , Plant Breeding , Musa/chemistry , Africa South of the Sahara , Cooperative Behavior
14.
Environ Res ; 252(Pt 1): 118785, 2024 Jul 01.
Article in English | MEDLINE | ID: mdl-38555094

ABSTRACT

The cube architecture associated with the CeO2 nanoflowers (NFs) that generated, which had an average crystallization width of 7 nm, has been confirmed by X-ray crystallographic investigations. The method used is environmentally acceptable since it converts wasted banana peel extracts into CeO2 nanoflower. On the basis of artwork obtained from a High-Resolution Transmission Electron Microscope (HR-TEM), CeO2 nanoparticles have been observed to possess a spherical shape and an average particle diameter of 21 nm. To take the purpose of this study, green-fabricated CeO2-NFs were used to investigate the photocatalytic oxidation of methyl orange (MO) dye when exposed to sunshine. CeO2 nanofibers showed a degradation performance of 98% when compared to methyl orange dye. Evidently is a possibility that this may be caused by the presence of CeO2 nanoflowers, whereby enhance the interaction of electrons, which are holes dissolution, and adherence. Upon a single day of being exposed, the biocidal potential was tested against both gram-positive and gram-negative bacteria, including E. coli, B. cereus, and S. aureus, among others. Due to the fact that its 32 mm minimum inhibitory concentration (MIC) for B. cereus was the highest among conventional medicines. As shown by the extraordinary capabilities of WBP@CeO2 tiny particles, manipulating of flexible tiny particles to feed the purpose of achieving effective and customizable infections and dermatologist advancements is really stunning.


Subject(s)
Anti-Bacterial Agents , Azo Compounds , Cerium , Musa , Plant Extracts , Musa/chemistry , Cerium/chemistry , Cerium/pharmacology , Azo Compounds/chemistry , Plant Extracts/chemistry , Plant Extracts/pharmacology , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Coloring Agents/chemistry , Catalysis , Microbial Sensitivity Tests
15.
Int J Biol Macromol ; 264(Pt 1): 130462, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38423435

ABSTRACT

Banana starch has attracted significant attention due to its abundant content of resistant starch. This study aims to compare the multiscale structure and functional properties of banana starch obtained from five cultivated varieties and investigate the impact of dielectric barrier discharge cold plasma (DBD) treatment on these starch characteristics. All five types of natural banana starch exhibited an elliptical and irregular shape, conforming to the CB crystal structure, with a bimodal distribution of branch chain lengths. The resistant starch content ranged from 88.9 % to 94.1 %. Variations in the amylose content, amylopectin branch chain length distribution, and structural characteristics resulted in differences in properties such as gelatinization behavior and sensitivity to DBD treatment. The DBD treatment inflicted surface damage on starch granules, reduced the amylose content, shortened the amylopectin branch chain length, and changed the relative crystallinity to varying degrees. The DBD treatment significantly increased starch solubility and light transmittance. Simultaneously, it resulted in a noteworthy decrease in peak viscosity and gelatinization enthalpy of starch paste. The in vitro digestibility test showed that 76.2 %-86.5 % of resistant starch was retained after DBD treatment. The DBD treatment renders banana starch with reduced viscosity, increased paste transparency, enhanced solubility, and broadens its potential application.


Subject(s)
Musa , Plasma Gases , Starch/chemistry , Amylopectin/chemistry , Amylose/chemistry , Musa/chemistry , Plasma Gases/chemistry , Resistant Starch , Viscosity
16.
Microsc Res Tech ; 87(7): 1479-1493, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38407375

ABSTRACT

For many years, the synthesis of graphene oxide (GO) had involved exfoliating graphite flakes, and the methods applied were expensive and time-consuming. Thus, an attempt had been made to create an inventive, less expensive method for the synthesis of GO using unrefined, raw carbon-containing material. Modified Hummer's method was used to prepare GO from banana peel. In addition, the metallic silver nanocomposite was also synthesized along with laoding of drug Rocephin where they interact with each other through electrostatic hydrogen bond interaction. The degree of crystallinity and the crystallite size were through x-ray diffraction (XRD) analysis and the crystallite size of AgNPs was found to be 40.40 nm. The scanning electron microscopy (SEM) analysis shows that the morphology of the GO gradually changes with the addition of AgNPs and Rocephin. A blue shift was seen in the absorbance maxima of the raw carbon upon the conjugation of Rocephin in UV analysis. The Fourier-transform infrared spectroscopy, and energy dispersive X-ray (EDX) spectroscopy were used to determine the chemical composition of the samples. Furthermore, a broad biological screening of the synthesized samples had been carried out following the total reducing power (TRP), total antioxidant capacity (TAC), antibacterial, antifungal, MTT (Cytotoxicity of biologically synthesized silver nanoparticles in MDA-MB-231 human breast cancer cells) cell viability, brine shrimp lethality, and hemolytic protocols. Significant results were obtained, and the Rocephin-GO-AgNPs had depicted promising activity as compared with their counterparts. RESEARCH HIGHLIGHTS: The GO was prepared from the raw carbon extracted from banana peels and was used as a substrate for the synthesis Graphene oxide silver nanoparticles (GO-AgNPs) and Rocephin-loaded graphene oxide silver nanoparticles (Rocephin-GO-AgNPs) The structural and compositional analysis of the nanomaterial was carried out, and they were screened for several biomedical applications. The Rocephin-GO-AgNPs exhibit the highest activity as compared with their counterparts.


Subject(s)
Graphite , Metal Nanoparticles , Nanocomposites , Silver , Graphite/chemistry , Silver/chemistry , Silver/pharmacology , Nanocomposites/chemistry , Animals , Metal Nanoparticles/chemistry , Humans , Artemia/drug effects , X-Ray Diffraction , Spectroscopy, Fourier Transform Infrared , Antioxidants/pharmacology , Antioxidants/chemistry , Anti-Bacterial Agents/pharmacology , Microscopy, Electron, Scanning , Musa/chemistry , Anti-Infective Agents/pharmacology
17.
Int J Biol Macromol ; 254(Pt 1): 127748, 2024 Jan.
Article in English | MEDLINE | ID: mdl-38287591

ABSTRACT

Banana starch has a highly resistant starch (RS) and slow-digested starch (SDS) content, making it attractive as a functional ingredient. Unfortunately, banana starch requires modification processes due to the loss of RS and SDS during gelatinization because of its thermolabile characteristics. This study explores the effect of banana starch modification by enzymatic, heat moisture treatment (HMT) and dual modification (HMT+ enzymatic) on its nutritional (RS, SDS) and functional properties (hydration, structural, gelation, rheological). HMT and dual modifications decrease RS (from 44.62 g/100 g to 16.62 and 26.66 g/100 g, respectively) and increase SDS (from 21.72 g/100 g to 33.91 and 26.95 g/100 g, respectively) in raw starch but induce structural changes that enhance RS (from 3.10 g/100 g to 3.94 and 4.4 g/100 g, respectively) and SDS (from 2.58 g/100 g to 9.58 and 11.48 g/100 g) thermo-resistance in gelled starch. Also, changes in the functional properties of starches were evidenced, such as weaker gels (hardness < 41 g), lower water absorption (<12.35 g/g), high starch solubility (>1.77 g/100 g) and increased gelatinization temperature. Improved gelatinization temperature and RS thermostability resulted from modifications that could expand banana starch applications as a beverage and compote thickener agent.


Subject(s)
Musa , Starch , Starch/chemistry , Musa/chemistry , Chemical Phenomena , Solubility , Temperature , Resistant Starch , Hot Temperature
18.
J Ethnopharmacol ; 322: 117639, 2024 Mar 25.
Article in English | MEDLINE | ID: mdl-38135229

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: The stem of Musa paradisiaca (plantain) has found application in traditional medicine for the treatment of diabetes, inflammation, ulcers and wound injuries. AIM OF THE STUDY: This study investigated the phytochemical composition, toxicity profile, wound healing, anti-inflammatory and analgesic effects of aqueous Musa paradisiaca stem extract (AMPSE) in rats. METHODS: Phytochemical analysis of methanol-MPSE was performed by gas chromatography-mass spectrometry (GC-MS). Acute toxicity testing was carried out through oral administration of a single dose of AMPSE up to 5 g/kg. Four separate groups of rats were used for the subacute toxicity testing (n = 6). Group 1 served as a normal control and did not receive AMPSE, groups 2-4 received AMPSE daily by gavage for 28 days. In the experiments with excision and incision wounds, the rats were treated with 10 w/w AMPS extract. The anti-inflammatory and analgesic effects of AMPSE were assessed using egg albumin-induced paw oedema and acetic acid-induced writhing methods, respectively. For the subacute, anti-inflammatory and analgesic studies, AMPSE was administered to the experimental rats at doses of 300, 600 and 900 mg/kg body weight. RESULTS: Bioactive compounds identified include ß-sitisterol, n-hexadecanoic acid, octadecanoic acid, diethyl sulfate, p-hydroxynorephedrine, phenylephrine, nor-pseudoephedrine, metaraminol, pseudoephedrine and vanillic acid. No signs of toxicity and no deaths were observed in all the groups. For the groups treated with AMPSE for 28 days, a significant reduction in alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, urea, sodium, chloride, total cholesterol, triglycerides, and low-density lipoprotein cholesterol were observed while high density lipoprotein cholesterol, glutathione and superoxide dismutase increased compared to control (p < 0.05). In wound healing experiments, AMPSE showed greater percent wound contraction and wound resistance fracture compared to the povidone-iodine (PI) treated and control groups. Treatment with 900 mg/kg AMPSE resulted in significant (p < 0.05) anti-inflammatory and analgesic effects compared to the control. CONCLUSION: This study shows that AMPSE is not toxic but contains biologically active compounds with hepatoprotective, anti-inflammatory, lipid-lowering and wound-healing effects. Treatment of rats with AMPSE has shown that AMPSE has anti-inflammatory, analgesic, hepatoprotective, lipid-lowering and wound-healing effects, supporting its therapeutic use in ethnomedicine.


Subject(s)
Musa , Musaceae , Plantago , Rats , Animals , Musa/chemistry , Plant Extracts/therapeutic use , Plant Extracts/toxicity , Pseudoephedrine/pharmacology , Analgesics/therapeutic use , Analgesics/toxicity , Anti-Inflammatory Agents/therapeutic use , Anti-Inflammatory Agents/toxicity , Wound Healing , Cholesterol/pharmacology , Phytochemicals/therapeutic use , Phytochemicals/toxicity , Lipids/pharmacology
19.
Int J Biol Macromol ; 258(Pt 2): 128989, 2024 Feb.
Article in English | MEDLINE | ID: mdl-38154717

ABSTRACT

Pectin is a polysaccharide mainly used in food processing industries as an emulsifier, thickener, stabilizer and in pharmaceuticals as an excipient, wall material and bio adhesive for improving delivery and efficiency. Raising demand for pectin, pushes to explore unconventional plant-based sources for the extraction of pectin. This work is aimed to explore the possibility of extracting pectin from the peel of banana varieties and to decipher the chemical and techno-functional properties. Among the varieties, Nendran, a plantain banana recorded higher pectin recovery (23.42 %), swelling power (23.10 gg-1), anhydrouronic acid (AUA) content (72.86 %) and emulsifying activity (46.19 %). Pectin from the banana peels exhibited the equivalent weight (g/mol) ranging from 943.40 (var. Bhimkol) to 1282.05 (var. Nendran). Morphological observations revealed that the extracted pectin has fragments with uneven sizes and inter-particle voids in the structure. Banana pectin behaved similar to commercial pectin in terms of rheological, textural and structural profiles. HPLC analysis and NMR spectra confirmed the dominance of galacturonic acid in the banana peel pectins. The study unveiled and opened up the avenues of utilizing banana peel as a complementary biomass for the extraction of pectin which could be used in different industrial applications.


Subject(s)
Musa , Pectins , Pectins/chemistry , Musa/chemistry , Polysaccharides , Emulsifying Agents/chemistry
20.
ScientificWorldJournal ; 2023: 8898045, 2023.
Article in English | MEDLINE | ID: mdl-37808477

ABSTRACT

Natural polymers such as pectin have gained increased utilization in pharmaceutical and biotechnology sectors because they are affordable, easily accessible, nontoxic, and chemically modifiable, with the potential to be biodegradable and biocompatible. Musa paradisiaca (plantain) peels make up 30-40% of the overall weight of the fruit. The extraction of pectin from these residues can therefore be viewed as a possible waste of wealth. This study, therefore, focused on evaluating the suspending properties of pectin obtained from Musa paradisiaca (plantain) peels (through acid and alkaline extraction) and presented an alternative suspending agent in the pharmaceutical formulation of suspensions. The unripe peels of Musa paradisiaca were acquired and authenticated at the Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana. Pectin was extracted from the peels using both acid and alkaline extraction processes, respectively, characterized, and evaluated for its phytochemical properties. Different concentrations of the acid and alkaline pectin extracts were employed as a suspending agent in paracetamol suspensions, using acacia gum as a standard. The pectin yields obtained were 4.88% and 7.61% for the acid and alkaline extraction processes, respectively, while phytochemical screening revealed the presence of glycosides, tannins, saponins, and phenols in both extracts. The alkaline pectin extract recorded higher equivalent weight, degree of esterification, ash content, and crude content than the acid pectin extract, while FTIR identified similar functional groups in both acid and alkaline pectin extracts. The test suspensions reported significant differences (P < 0.05) in flow rates, ease of redispersion, sedimentation volumes, and rates compared with acacia gum. Moreover, when the acid and alkaline pectin extracts were compared, significant differences (P < 0.05) were observed in sedimentation rates and sedimentation volumes, suggesting that the extraction method may affect suspending properties. Ultimately, the alkaline pectin extract had better suspending properties than the acid pectin extract; however, they both can be used as an alternative to acacia gum as a suspending agent.


Subject(s)
Musa , Pharmacy , Plantago , Pectins , Excipients/chemistry , Musa/chemistry , Drug Compounding , Gum Arabic , Phytochemicals
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