ABSTRACT
The effect of a single intramuscular injection of prednisolone sodium phosphate (14.3 mg/kg) on the development and severity of dermal lesions induced by topical sulfur mustard was investigated in guinea pigs. Control animals received sulfur mustard alone, topical sulfur mustard and intramuscular saline, intramuscular prednisolone alone, or intramuscular saline alone. With topical sulfur mustard alone there were typical mustard lesions consisting of epidermal necrosis and hyalinization, with acute inflammation and necrosis in the dermis, the severity and extent of which were dose-related. Systemic prednisolone therapy had no influence on the nature, severity or progression of the dermal histopathologic response to topical sulfur mustard.
Subject(s)
Mustard Compounds/antagonists & inhibitors , Mustard Gas/antagonists & inhibitors , Prednisolone/therapeutic use , Skin Diseases/chemically induced , Animals , Guinea Pigs , Injections, Intramuscular , Necrosis , Neutrophils , Prednisolone/administration & dosage , Skin/pathology , Skin Diseases/pathologySubject(s)
Methylamines/antagonists & inhibitors , Mustard Compounds/antagonists & inhibitors , Naphthalenes/antagonists & inhibitors , Tryptophan/pharmacology , Animals , Dose-Response Relationship, Drug , Guinea Pigs , Histidine/pharmacology , Male , Muscle Contraction/drug effects , Norepinephrine/antagonists & inhibitors , Phenylephrine/pharmacology , Receptors, Drug/drug effects , Tyrosine/pharmacology , Vas Deferens/drug effectsABSTRACT
1 The synthesis of tritium labelled propylbenzilylcholine mustard ([(3)H]-PrBCM; N-2'-chloroethyl-N-[2'', 3''-(3)H(2)] propyl-2-aminoethyl benzilate) is described.2 The uptake by muscle strips was measured and shown to be considerably increased by previous immersion of the muscle in distilled water.3 A considerable part of the uptake is inhibited selectively by atropine, but not by nicotinic antagonists. A number of muscarinic agonists also inhibit uptake and their apparent affinity constants have been determined.4 The uptake by atropine-sensitive sites is temperature-insensitive, whereas the other sites are temperature-sensitive. Recovery is highly temperature-sensitive and there is good agreement between recovery of sensitivity to agonists and loss of radioactivity from the muscle.
