1.
Nature
; 542(7640): 166, 2017 02 08.
Article
in English
| MEDLINE
| ID: mdl-28179651
Subject(s)
Molecular Biology/history , Mutagenesis, Site-Directed/history , Anemia, Sickle Cell/genetics , Anemia, Sickle Cell/therapy , Animals , Cystic Fibrosis , Disease Models, Animal , Genetic Therapy/history , Genetic Therapy/methods , Haptoglobins/chemistry , History, 20th Century , History, 21st Century , Humans , Mice , Mouse Embryonic Stem Cells/metabolism , Mutagenesis, Site-Directed/methods , Nobel Prize , United Kingdom , United States
2.
Biotechniques
; 54(3): 127-8, 2013 Mar.
Article
in English
| MEDLINE
| ID: mdl-23477381
ABSTRACT
Gene Splicing by Overlap Extension (SOE), described in a BioTechniques Research Report 23 years ago, is still widely used today in spite of changes in technology and scientific fashion. The history of SOE offers practical lessons for those who seek the best techniques, and for those who strive to develop them.
Subject(s)
DNA, Recombinant/genetics , Polymerase Chain Reaction/methods , Animals , DNA Primers/genetics , History, 20th Century , History, 21st Century , Humans , Mutagenesis, Site-Directed/history , Mutagenesis, Site-Directed/methods , Polymerase Chain Reaction/history
3.
EMBO J
; 30(12): 2312-24, 2011 May 20.
Article
in English
| MEDLINE
| ID: mdl-21602790
Subject(s)
Genetic Code , Protein Engineering/history , Protein Engineering/methods , Protein Processing, Post-Translational/genetics , Amino Acyl-tRNA Synthetases/genetics , Amino Acyl-tRNA Synthetases/history , Aminoacylation/genetics , Animals , History, 20th Century , History, 21st Century , Mutagenesis, Site-Directed/history , Mutagenesis, Site-Directed/methods , Protein Interaction Mapping/history , Protein Interaction Mapping/methods , Ribosomes/enzymology , Ribosomes/genetics
4.
Pillars article: a critical role of lambda 5 protein in B cell development. Cell. 1992. 69: 823-831.
J Immunol
; 184(3): 1130-8, 2010 Feb 01.
Article
in English
| MEDLINE
| ID: mdl-20089708
Subject(s)
B-Lymphocytes/immunology , Hematopoietic Stem Cells/immunology , Immunoglobulin Light Chains, Surrogate/history , Immunoglobulin lambda-Chains/history , Animals , B-Lymphocytes/cytology , Base Sequence , Cell Differentiation/genetics , Gene Rearrangement, B-Lymphocyte , Hematopoietic Stem Cells/cytology , History, 20th Century , Humans , Immunoglobulin Light Chains, Surrogate/genetics , Immunoglobulin lambda-Chains/genetics , Mice , Mice, Inbred Strains , Mice, Transgenic , Mutagenesis, Insertional , Mutagenesis, Site-Directed/history , Recombination, Genetic
5.
Rev Med Chir Soc Med Nat Iasi
; 112(2): 289-92, 2008.
Article
in Romanian
| MEDLINE
| ID: mdl-19294993