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1.
Article in English | MEDLINE | ID: mdl-24937569

ABSTRACT

Here I report the complete mitochondrial genome of the African palm civet, (Nandinia binotata) as sequenced from overlapping PCR products. The genome is 17,103 bp in length and contains the 37 genes found in a typical mammalian genome: 13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. The control region of N. binotata includes both RS2 and RS3 tandem repeats. The overall base composition on the L-strand is A: 33.6%, C: 27.3%, G: 13.0%, and T: 26.1%.


Subject(s)
DNA, Mitochondrial/genetics , Genome, Mitochondrial/genetics , Mitochondria/genetics , Nandiniidae/genetics , Sequence Analysis, DNA/veterinary , Africa , Animals , Base Composition/genetics , Base Sequence , Genome Size , Molecular Sequence Data , Polymerase Chain Reaction/veterinary , RNA, Ribosomal/genetics , RNA, Transfer/genetics , Tandem Repeat Sequences/genetics
2.
Nat Commun ; 5: 4106, 2014 Jun 30.
Article in English | MEDLINE | ID: mdl-24978855

ABSTRACT

Oil palm, a plantation crop of major economic importance in Southeast Asia, is the predominant source of edible oil worldwide. We report the identification of the virescens (VIR) gene, which controls fruit exocarp colour and is an indicator of ripeness. VIR is a R2R3-MYB transcription factor with homology to Lilium LhMYB12 and similarity to Arabidopsis production of anthocyanin pigment1 (PAP1). We identify five independent mutant alleles of VIR in over 400 accessions from sub-Saharan Africa that account for the dominant-negative virescens phenotype. Each mutation results in premature termination of the carboxy-terminal domain of VIR, resembling McClintock's C1-I allele in maize. The abundance of alleles likely reflects cultural practices, by which fruits were venerated for magical and medicinal properties. The identification of VIR will allow selection of the trait at the seed or early-nursery stage, 3-6 years before fruits are produced, greatly advancing introgression into elite breeding material.


Subject(s)
Fruit/growth & development , Gene Expression Regulation, Plant , Nandiniidae/metabolism , Plant Proteins/metabolism , Amino Acid Sequence , Animals , Fruit/genetics , Fruit/metabolism , Gene Expression Regulation, Developmental , Molecular Sequence Data , Nandiniidae/classification , Nandiniidae/genetics , Pancreatitis-Associated Proteins , Phylogeny , Plant Proteins/genetics , Plants/classification , Plants/genetics
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