ABSTRACT
Epidemiological data have shown that periodontal bacterial infection, periodontitis, and oral squamous cell carcinoma have close relationship on the disease progress and risk. However, the specific role of periodontal microbes and their mechanism in the development of oral squamous cell carcinoma is not yet clear. In our previous work, metagenomic Illumina Mi-seq analysis was used to identify tstructure and abundance of periodontital microbiome. Accoding to the results, we used Porphyromonas.spp. and Fusobacterium.spp. as the periodontitis positive microbiota; Neisseria.spp and Corynebacterium.spp as periodontitis negative microbiota (their average relative abundance were >5%). These representative strains of the above genus were used to infect OSCC cells to explore their effect on tumor cell biology behavior, and detect the expression level of the gene in related to inflammation, migration, invasion and cell cycle. We find that periodontitis positive correlated microbiota had a promoting effect on the development of oral squamous cell carcinoma in vitro by regulating mRNA and protein expression of IL-6, IL-8, MMP-9 and Cyclin-D1. Periodontitis negative correlated microbiota had suppression effect on the development of oral squamous cell carcinoma in vitro analysis.
Subject(s)
Head and Neck Neoplasms/microbiology , Microbiota , Periodontitis/microbiology , Squamous Cell Carcinoma of Head and Neck/microbiology , Bacteroidaceae Infections/complications , Bacteroidaceae Infections/microbiology , Bacteroidaceae Infections/pathology , Cell Line, Tumor , Cell Movement/physiology , Corynebacterium/genetics , Corynebacterium/isolation & purification , Fusobacterium Infections/complications , Fusobacterium Infections/microbiology , Fusobacterium Infections/pathology , Fusobacterium nucleatum/genetics , Fusobacterium nucleatum/isolation & purification , Head and Neck Neoplasms/metabolism , Head and Neck Neoplasms/pathology , Humans , Neisseria sicca/genetics , Neisseria sicca/isolation & purification , Neisseriaceae Infections/complications , Neisseriaceae Infections/microbiology , Neisseriaceae Infections/pathology , Porphyromonas gingivalis/genetics , Porphyromonas gingivalis/isolation & purification , Squamous Cell Carcinoma of Head and Neck/metabolism , Squamous Cell Carcinoma of Head and Neck/pathologyABSTRACT
The study of the interaction of bacteria with surfaces requires the detection of specific bacterial groups with high spatial resolution. Here, we describe a method to rapidly and efficiently add nanogold particles to oligonucleotide probes, which target bacterial ribosomal RNA. These nanogold-labeled probes are then used in an in situ hybridization procedure that ensures both cellular integrity and high specificity. Electron microscopy subsequently enables the visualization of specific cells with high local precision on complex surface structures. This method will contribute to an increased understanding of how bacteria interact with surface structures on a sub-micron scale.
Subject(s)
Bacteria/genetics , Bacteriological Techniques/methods , Microscopy, Electron, Scanning/methods , Nanoparticles/chemistry , Bacteria/isolation & purification , Bacteriological Techniques/instrumentation , Escherichia coli/genetics , Escherichia coli/isolation & purification , Gold/chemistry , In Situ Hybridization/methods , Neisseria sicca/genetics , Neisseria sicca/isolation & purification , Oligonucleotide Probes/chemical synthesis , Oligonucleotide Probes/chemistry , Oligonucleotide Probes/genetics , RNA, Bacterial , RNA, Ribosomal , Sensitivity and Specificity , Spectrometry, X-Ray EmissionABSTRACT
We report a case of invasive Neisseria sicca/subflava meningitis after a spinal injection procedure during which a face mask was not worn by the proceduralist. The report highlights the importance of awareness of, and adherence to, guidelines for protective face mask use during procedures that require sterile conditions.
Subject(s)
Infectious Disease Transmission, Professional-to-Patient , Neisseria sicca/isolation & purification , Neisseriaceae Infections/transmission , Aged , Contrast Media/administration & dosage , Humans , Iatrogenic Disease , Injections, Spinal , Male , Neisseria sicca/growth & development , Neisseriaceae Infections/microbiologyABSTRACT
We report the first case, in Medline-based literature, of conjunctivitis caused by gram negative diplococcus, Neisseria sicca. Although it is not widely accepted as such, isolation from cultures of repeated eye swab samples suggests that N. sicca may be a pathogen in conjunctival infections. Positive culture for this organism should not be readily dismissed. Such conjunctivitis responded favorably to treatment with netilmicin eye drops.
Subject(s)
Conjunctiva/microbiology , Conjunctivitis/microbiology , Eye Infections, Bacterial/microbiology , Neisseria sicca/isolation & purification , Neisseriaceae Infections/microbiology , Anti-Bacterial Agents/administration & dosage , Conjunctiva/pathology , Conjunctivitis/diagnosis , Conjunctivitis/drug therapy , Eye Infections, Bacterial/diagnosis , Eye Infections, Bacterial/drug therapy , Humans , Male , Neisseriaceae Infections/diagnosis , Neisseriaceae Infections/drug therapy , Ophthalmic SolutionsSubject(s)
Discitis/microbiology , Endocarditis, Bacterial/microbiology , Neisseria sicca/isolation & purification , Neisseriaceae Infections/microbiology , Thoracic Vertebrae/microbiology , Aged , Anti-Bacterial Agents/therapeutic use , Aortic Valve/microbiology , Ceftriaxone/therapeutic use , Ciprofloxacin/therapeutic use , Discitis/drug therapy , Endocarditis, Bacterial/drug therapy , Humans , Intervertebral Disc Degeneration/complications , Male , Neisseria sicca/enzymology , Neisseriaceae Infections/drug therapy , Penicillinase/metabolismSubject(s)
Endocarditis, Bacterial/diagnosis , Endocarditis, Bacterial/pathology , Neisseria sicca/isolation & purification , Neisseriaceae Infections/diagnosis , Neisseriaceae Infections/pathology , Aged , Autopsy , Blood/microbiology , Brain/diagnostic imaging , Brain/pathology , Cerebellum/pathology , Conjunctiva/pathology , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Endocarditis, Bacterial/microbiology , Exanthema/pathology , Fatal Outcome , Female , Humans , Kidney/diagnostic imaging , Kidney/pathology , Lung/diagnostic imaging , Lung/pathology , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Tomography, X-Ray Computed , UltrasonographyABSTRACT
Neisseria sicca/subflava are generally considered commensal inhabitants of the human oropharynx. We describe a case of disseminated N. sicca/subflava infection in an immunocompromised 15-year-old male presenting with cutaneous erythematous nodules. Our report adds to the growing evidence that these bacteria can cause disseminated infections, and describes a cutaneous manifestation of disseminated disease with N. sicca/subflava.
Subject(s)
Bacteremia/complications , Bacteremia/diagnosis , Neisseria sicca/isolation & purification , Neisseriaceae Infections/diagnosis , Skin Diseases, Bacterial/microbiology , Adolescent , Anti-Bacterial Agents/therapeutic use , Antifungal Agents/therapeutic use , Bacteremia/microbiology , Bacteremia/pathology , Blood/microbiology , Humans , Immunocompromised Host , Male , Neisseriaceae Infections/microbiology , Skin/microbiology , Skin Diseases, Bacterial/pathology , Treatment OutcomeABSTRACT
Neisseria sicca is rarely associated with clinical infections and to the authors' knowledge this organism has not been reported as a causative agent of infected biloma (liver abscess). A case of a diabetic man with infected biloma due to N. sicca after repeated transcatheter arterial embolization for hepatocellular carcinoma is reported. The patient was successfully treated with intravenous cefotaxime and metronidazole. The biochemical profile and 16S rRNA gene partial sequencing results of the isolate were in agreement with those of N. sicca.
