ABSTRACT
Various preparations of iron(III) nitrilotriacetate (FeNTA) solution reported in the literature lack a comprehensive method for accurate determination of FeNTA concentration and often result in unstable solutions. A detailed procedure for the preparation of FeNTA solution is presented that includes the standardization of both components of the chelate. The standardization of the components allowed the accurate determination of the molar absorption coefficients for the calculation of the FeNTA concentration in two different buffers at pH 5.6 and 7.4. The variation of pH in this range or ionic strength in the range from 0 M to 3 M (KCl) has little effect on the value of the molar absorption coefficient. The precise concentrations of all species involved in the equilibria between Fe and NTA were determined in the pH range 2-12 using the Jenkins-Traub algorithm to solve the 5th-order polynomial in Microsoft Excel. In view of the experimental observations and the calculated distribution of species, the stability of FeNTA solutions may be affected by the Fe : NTA ratio and the total concentrations, with dilute solutions and those with an excess of NTA over Fe showing higher stability.
Subject(s)
Iron , Nitrilotriacetic Acid , Iron/metabolism , Protein Binding , Nitrilotriacetic Acid/chemistry , Nitrilotriacetic Acid/metabolismABSTRACT
Earlier we have shown that exposure to copper-nitrilotriacetate (Cu-NTA) manifests toxicity by generating oxidative stress and potent induction of proliferative reaction in the liver and kidney. In the study, we look at the impact of nitroglycerin (GTN) administration on Cu-NTA-induced oxidative stress and hyperproliferative response in the liver and kidney. GTN administration intraperitoneally to male Wistar rats after Cu-NTA administration intraperitoneally caused substantial protection against Cu-NTA-induced tissue injury, oxidative stress and hyperproliferative response. Cu-NTA administration at a dose of 4.5 mg/kg body weight produces significant (p < .001) elevation in biochemical parameters including aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and creatinine (CREA) with a concomitant increase in microsomal lipid peroxidation. Along with these alterations, we discovered a substantial increment in [3H]thymidine incorporation into hepatic and renal DNA synthesis (p < .001). Cu-NTA-induced tissue damage and lipid peroxidation in hepatic and renal tissues were inhibited by GTN treatment in a dose-dependent manner (p < .05-0.001). Furthermore, GTN can suppress the hyperproliferative response elicited by Cu-NTA by down-regulating the rate of [3H]thymidine incorporation into hepatic and renal DNA (p < .01-0.001). Protective effect of GTN against Cu-NTA was also confirmed by histopathological changes in liver and kidney. This result suggests that GTN may serve as a scavenger for reactive oxygen species (ROS) and reduces toxic metabolites of Cu-NTA, thereby avoiding tissue injury and oxidative stress. Further, administration of NO inhibitor, NG-Nitroarginine methyl ester (L-NAME), exacerbated Cu-NTA induced oxidative tissue damage and cell proliferation. Overall, GTN reduces Cu-NTA-induced tissue damage, oxidative stress, and proliferative response in the rat liver and kidney, according to these findings. On the basis of the above results, present study suggests that GTN may be a potential therapeutic agent for restoration of oxidative damage and proliferation to liver and kidney.
Subject(s)
Copper , Nitroglycerin , Rats , Animals , Male , Nitroglycerin/pharmacology , Copper/toxicity , Rats, Wistar , Kidney , Lipid Peroxidation , Nitrilotriacetic Acid/toxicity , Nitrilotriacetic Acid/metabolism , Oxidative Stress , Liver/metabolism , Antioxidants/pharmacology , NG-Nitroarginine Methyl Ester/pharmacology , Thymidine/metabolism , Thymidine/pharmacology , DNA/metabolism , Ferric Compounds/toxicityABSTRACT
Iron-catalyzed oxidative stress generates reactive oxygen species in the kidney and induces oxidative damage including lipid, protein, and DNA modifications which induces renal injury and may lead to cancer. An analysis of oxidative stress dynamics by reactive oxygen species has not been performed non-invasively in real time in intact kidneys and is a significant challenge in biology and medicine. Here, I report that MCLA-800 is a near-infrared chemiluminescent probe that visualizes the dynamics of superoxide anion (O2â¢-) production and the upstream generation of reactive oxygen species in living rat kidneys suffering acute renal oxidative stress induced by intraperitoneal administration of iron3+-nitrilotriacetate (Fe3+-NTA) as a representative Fe3+ chelate. MCLA-800 was intravenously injected at 250 nmol/kg body weight and immediately transported to the kidneys with the emitting light dependent on O2â¢- production. The magnitude of O2â¢- production correlated with the Fe3+-NTA dose. O2â¢- was continuously produced in the blood stream following Fe3+-NTA injection at 0.15 mmol/kg body weight, while peak production in the renal cortex occurred at 24 h, then decreased to the background level at 72 h. This study clearly revealed the dynamics of Fe3+-NTA-mediated O2â¢- production in the living kidney by chemiluminescent imaging of O2â¢- production using MCLA-800.
Subject(s)
Nitrilotriacetic Acid , Superoxides , Animals , Carcinogens/pharmacology , Ferric Compounds/metabolism , Iron/metabolism , Kidney , Lipid Peroxidation , Luminescence , Nitrilotriacetic Acid/metabolism , Nitrilotriacetic Acid/pharmacology , Oxidative Stress , Rats , Superoxides/metabolismABSTRACT
In the present research, the effects of sublethal lead (Pb) concentrations on total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) levels, enzyme activities (aspartate transaminase, AST; alanine transaminase, ALT; lactate dehydrogenase, LDH), ion levels (magnesium, Mg; sodium, Na; potassium, K; chlorine, Cl; calcium, Ca), and some metabolite levels (cholesterol, triglyceride, HDL, LDL, albumin, total protein) in the blood serum of Oreochromis niloticus and the protective function of nitrilotriacetic acid (NTA) due to its chelating characteristic were investigated. O. niloticus, which has an important position in the food chain and is often preferred in toxicological studies, was exposed to 0.1 ppm Pb, 0.1 ppm Pb + 0.3 ppm NTA, 1 ppm Pb, and 1 ppm Pb + 3 ppm NTA concentrations for 7 and 21 days. At the end of the duration, serum TAS and TOS levels were measured spectrophotometrically with Rel Assay Diagnostics; other enzyme activities, ion levels, and metabolite parameters were done by an autoanalyzer using commercial kits. Depending on the exposure periods and concentrations, the changes in the parameters were determined. It is determined that, under the influence of high ambient concentration of lead, TOS, OSI, AST, ALT, LDH, LDL, triglyceride, and Mg levels increased, while TAS, albumin, and K levels decreased after 21 days. These increases/decreases in all serum biochemical parameters were generally higher in fish treated with Pb alone compared to fish treated with a mixture of Pb + NTA. This study shows that these changes in serum parameters could be used as an indicator to assess on metal toxicity.
Subject(s)
Cichlids , Albumins/metabolism , Animals , Antioxidants/metabolism , Chelating Agents/metabolism , Chelating Agents/pharmacology , Chlorides/pharmacology , Cichlids/metabolism , Lead/metabolism , Nitrilotriacetic Acid/metabolism , Nitrilotriacetic Acid/toxicity , Oxidative Stress , TriglyceridesABSTRACT
Amyloid-ß (Aß) peptide and tau protein are two hallmark proteins in Alzheimer's disease (AD); however, the parameters, which mediate the abnormal aggregation of Aß and tau, have not been fully discovered. Here, we have provided an optimum method to purify tau protein isoform 1N4R by using nickel-nitrilotriacetic acid agarose chromatography under denaturing condition. The biochemical and biophysical properties of the purified protein were further characterized using in vitro tau filament assembly, tubulin polymerization assay, circular dichroism (CD) spectroscopy and atomic force microscopy. Afterwards, we investigated the effect of tau protein on aggregation of Aß (25-35) peptide using microscopic imaging and cell viability assay. Incubation of tau at physiologic and supra-physiologic concentrations with Aß25-35 for 40 days under reducing and non-reducing conditions revealed formation of two types of aggregates with distinct morphologies and dimensions. In non-reducing condition, the co-incubated sample showed granular aggregates, while in reducing condition, they formed annular protofibrils. Results from cell viability assay revealed the increased cell viability for the co-incubated sample. Therefore, the disassembling action shown by tau protein on Aß25-35 suggests the possibility that tau may have a protective role in preventing Aß peptide from acquiring the cytotoxic, aggregated form against oxidative stress damages.
Subject(s)
Alzheimer Disease/metabolism , Amyloid beta-Peptides/metabolism , Peptide Fragments/metabolism , tau Proteins/metabolism , Alzheimer Disease/pathology , Amyloid beta-Peptides/chemistry , Cell Survival , Chromatography, Agarose/methods , Circular Dichroism/methods , Humans , Microscopy, Atomic Force , Nitrilotriacetic Acid/metabolism , Oxidative Stress , Peptide Fragments/chemistry , Protein Aggregates , Protein Aggregation, Pathological/metabolism , Protein Isoforms/metabolism , Spectrum Analysis/methods , tau Proteins/chemistry , tau Proteins/isolation & purificationABSTRACT
Specific and expeditious identification and enrichment of target proteins in living cells is often a challenging task. The hexahistidine (6His) tag is frequently used to label artificially engineered proteins produced in prokaryotic or eukaryotic cells. Utilizing the interaction between 6His-tag and nitrilotriacetic acid (NTA) mediated by divalent metal ions (Ni2+, Cu2+, Zn2+ or Co2+), we designed and synthesized a series of Nap-G/Biotin/ANA-FFpYGK-NTA probes that, assisted by alkaline phosphatase (ALP), self-assemble into nanofibers. The probe consists of an NTA group that specifically binds to 6His-tag, an FFpY group that promotes self-assembly facilitated by ALP, and a hydrophobic (Nap-G/ANA/Biotin) capping group for various applications. We demonstrate that the ANA-FFpYGK-NTA(Ni2+) nanofibers are fit for real-time tracking of His-tagged protein in living cells, and the Biotin-FFpYGK-NTA(Ni2+) nanofibers are for isolating His-tagged proteins and other proteins that they interact with.
Subject(s)
Chelating Agents/metabolism , Cytoplasm/metabolism , Histidine/metabolism , Nanofibers , Nitrilotriacetic Acid/metabolism , Oligopeptides/metabolism , Chelating Agents/analysis , Cytoplasm/chemistry , Fluorescent Dyes/analysis , Fluorescent Dyes/metabolism , Histidine/analysis , Humans , MCF-7 Cells , Nanofibers/analysis , Nitrilotriacetic Acid/analysis , Oligopeptides/analysisABSTRACT
The ability to incorporate a desired functionality into proteins of interest in a site-specific manner can provide powerful tools for investigating biological systems and creating therapeutic conjugates. However, there are not any universal methods that can be applied to all proteins, and it is thus important to explore the chemical strategy for protein modification. In this paper, we developed a new reactive peptide tag/probe pair system for site-specific covalent protein labeling. This method relies on the recognition-driven reaction of a peptide tag and a molecular probe, which comprises the lysine-containing short histidine tag (KH6 or H6K) and a binuclear nickel (II)- nitrilotriacetic acid (Ni2+-NTA) complex probe containing a lysine-reactive N-acyl-N-alkyl sulfonamide (NASA) group. The selective interaction of the His-tag and Ni2+-NTA propeles a rapid nucleophilic reaction between a lysine residue of the tag and the electrophilic NASA group of the probe by the proximity effect, resulting in the tag-site-specific functionalization of proteins. We characterized the reactive profile and site-specificity of this method using model peptides and proteins in vitro, and demonstrated the general utility for production of a nanobody-chemical probe conjugate without compromising its binding ability.
Subject(s)
Histidine/chemistry , Indicators and Reagents/chemistry , Molecular Probes/chemistry , Proteins/chemistry , Staining and Labeling , Sulfonamides/chemistry , HEK293 Cells , Histidine/metabolism , Humans , Indicators and Reagents/metabolism , Lysine/chemistry , Lysine/metabolism , Models, Molecular , Molecular Probes/metabolism , Molecular Structure , Nickel/chemistry , Nickel/metabolism , Nitrilotriacetic Acid/chemistry , Nitrilotriacetic Acid/metabolism , Proteins/metabolism , Sulfonamides/metabolismABSTRACT
Protein dynamics is at the heart of all cellular processes. Here, we utilize the dHis-CuII NTA label to obtain site-specific information on dynamics for both an α-helix and ß-sheet site of GB1, the immunoglobulin binding domain of protein G. Spectral features found in our CW-EPR measurements were consistent with the overall rigid nature of GB1 and with predictions from molecular dynamics simulations. Using this information, we show the potential of this approach to elucidate the role of dynamics in substrate binding of a functionally necessary α-helix in human glutathione transferase A1-1 (hGSTA1-1). We observe two dynamical modes for the helix. The addition of the inhibitor GS-Met and GS-Hex resulted in hGSTA1-1 to favor the more rigid active state conformation, while the faster mode potentially aids the search for substrates. Together the results illustrate the remarkable potential of the dHis-based labelling approach to measure site-specific dynamics using room temperature lineshape analysis.
Subject(s)
Glutathione Transferase/chemistry , Histidine/chemistry , Isoenzymes/chemistry , Molecular Dynamics Simulation , Temperature , Coordination Complexes/chemistry , Coordination Complexes/metabolism , Copper/chemistry , Copper/metabolism , Electron Spin Resonance Spectroscopy , Glutathione Transferase/metabolism , Histidine/metabolism , Humans , Isoenzymes/metabolism , Molecular Conformation , Nitrilotriacetic Acid/chemistry , Nitrilotriacetic Acid/metabolismABSTRACT
To effective capture and universal enrichment of His-tagged protein, polyacrylic acid (PAA) brushes were used to encapsulate Fe3O4 nanoparticles, connect NTA, and Ni2+ to prepare magnetic beads. These materials provide many advantages, such as excellent stability, tuneable particle size, and a surface for further functionalisation with biomolecules. His-tagged green fluorescence protein (GFP) was separated efficiently, and the binding capacity of Fe3O4/MPS@PAA/NTA-Ni2+ was 93.4â mg/g. Compared with High-Affinity Ni-NTA Resin and Ni-NTA Magnetic Agarose Beads, Fe3O4/MPS@PAA/NTA-Ni2+ nanocomposites exhibited higher separation efficiency and binding capacity towards His-tagged GFP. Moreover, the selectivity and recyclability of them for the target proteins were maintained well after six cycles. This study would widen the application of PAA in constructing multifunctional nanocomposites for biomedical fields.
Subject(s)
Histidine/metabolism , Magnetite Nanoparticles/chemistry , Nitrilotriacetic Acid/analogs & derivatives , Organometallic Compounds/chemistry , Recombinant Fusion Proteins/isolation & purification , Acrylic Resins/chemistry , Green Fluorescent Proteins/chemistry , Green Fluorescent Proteins/isolation & purification , Histidine/chemistry , Nanocomposites/chemistry , Nitrilotriacetic Acid/chemistry , Nitrilotriacetic Acid/metabolism , Organometallic Compounds/metabolism , Recombinant Fusion Proteins/chemistryABSTRACT
Nitrilotriacetic acid (NTA), a biodegradable chelant, has been promoted to effectively assist Pb phytoextraction, while a few researches available on the phytostabilizer of Athyrium wardii (Hook.). In this study, two incubation experiments and a subsequent column experiment were conducted to investigate the effects of application of NTA on Pb availability in soils and Pb accumulation in A. wardii and associated leaching risk. The application of NTA significantly increased the exchangeable Pb and Pb bound to carbonates along with a decreased pH, leading to enhanced Pb availability in soils. It was more effective in enhancing Pb availability in soils by adding 2 mmol kg-1 NTA into soils at once for 7 d, thus demonstrating potential for enhancing Pb uptake by A. wardii. After the addition of 2 mmol kg-1 NTA for 7 d, Pb concentrations in roots of A. wardii was enhanced by 23.8%, along with 10.6% of increase for Pb accumulation in roots. No significant changes were observed for the biomass of A. wardii. Meanwhile, the available Pb and TCLP-extractable Pb in 0-20 cm soils increased by 11.1-23.4% and 7.1-31.2%, thus promoting Pb leaching in 0-20 cm soils. However, there were no changes for Pb leaching risk levels of 20-40 cm soils. No Pb was detected in the leachates from all columns. The application of 2 mmol kg-1 NTA at once for 7 d is therefore proved to show greater potential in enhancing Pb remediation efficiency by the phytostabilizer of A. wardii without increasing Pb leaching risk into groundwater.
Subject(s)
Biodegradation, Environmental , Lead/metabolism , Soil Pollutants/metabolism , Tracheophyta/physiology , Biomass , Lead/analysis , Nitrilotriacetic Acid/metabolism , Plant Roots/metabolism , Soil , Soil Pollutants/analysis , Tracheophyta/metabolismABSTRACT
Fluorescence labeling of the target molecules using a small molecule-based probe is superior than a method using genetically expressed green fluorescence protein (GFP) in terms of convenience in its preparation and functionalization. Fluorophore-nitrilotriacetic acid (NTA) conjugates with several ester protecting groups were synthesized and evaluated for their cell membrane permeability by fluorescence microscopy analysis. One of the derivatives, acetoxymethyl (AM)-protected NTA conjugate is hydrolyzed, resulting in intracellular accumulation, thus providing localized fluorescence intensity in cells. This modification is expected as an effective method for converting a non-cell membrane permeable NTA-BODIPY conjugates to a cell membrane permeable derivatives.
Subject(s)
Fluorescent Dyes/chemistry , Fluorescent Dyes/metabolism , Nitrilotriacetic Acid/analogs & derivatives , Nitrilotriacetic Acid/metabolism , Boron Compounds/chemical synthesis , Boron Compounds/chemistry , Boron Compounds/metabolism , Cell Line , Cell Membrane Permeability , Fluorescence , Fluorescent Dyes/chemical synthesis , Humans , Hydrolysis , Microscopy, Fluorescence , Nitrilotriacetic Acid/chemical synthesisABSTRACT
Nontransferrin-bound iron (NTBI) is a heterogeneously speciated plasma iron, typically detectable when transferrin saturation (TfSat) exceeds 75%. Here, we examine factors affecting NTBI levels by a recently discovered direct chelator-based (CP851) fluorescent bead-linked flow-cytometric assay (bead-NTBI), compared with the established indirect nitrilotriacetate (NTA) assay in 122 iron-overloaded patients, including 64 on recent iron chelation therapy and 13 healthy volunteers. Both methods correlated (r = 0.57, P < 0.0001) but with low agreement, attributable to 2 major factors: (1) the NTA method, unlike the bead method, is highly dependent on TfSat, with NTBI under-estimation at low TfSat and over-estimation once Tf is saturated, (2) the bead method detects <3-fold higher values than the NTA assay in patients on recent deferiprone-containing chelation due to greater detection of chelate complexes but lower values for patients on deferasirox. The optimal timing of sample collection relative to chelation dosing requires further study. Patients with splenectomy, high-storage iron, and increased erythropoiesis had greater discrepancy between assays, consistent with differential access by both methods to the NTBI pools associated with these clinical variables. The bead-NTBI assay has advantages over the NTA assay, being less dependent on TfSat, hence of less tendency for false-negative or false-positive values at low and high TfSat, respectively.
Subject(s)
Biological Assay/methods , Iron/metabolism , Microspheres , Transferrin/metabolism , Fluorescence , Humans , Iron Chelating Agents/pharmacology , Nitrilotriacetic Acid/metabolism , Regression AnalysisABSTRACT
Phytoextraction has been identified as one of the most propitious methods of phytoremediation. This pot experiment were treated with varying amounts of (ethylenediamine triacetic acid) EDTA 3-15, (Nitriloacetic acid) NTA 3-10, (Ammonium citrate) NH4 citrate 10 - 25 mmol and one mg kg(-1)Cd, filled with 5 kg soil. The addition of chelators significantly increased Cd concentration in soil and plant. The results showed that maximum Cd uptake was noted under root, shoot and leaf of castor plant tissue (2.26, 1.54, and 0.72 mg kg(-1)) under EDTA 15, NTA 10, and NH4 citrate 25 mmol treatments respectively, and in soil 1.08, 1.06 and 0.52 mg kg(-1) pot(-1) under NH4 citrate 25, NTA 10 and EDTA 15 mmol treatments respectively, as against to control (p < 0.05). Additions of chelators reduction biomass under the EDTA 15 mmol as compared to other treatments, However, Bioconcentration factor (BCF), translocation factor (TF) and remediation factor (RF) were significantly increased under EDTA 15 and NH4 citrate 25 mmol as against control. Our results demonstrated that castor plant proved satisfactory for phytoextraction on contaminated soil, and EDTA 15 and NH4 citrate 25 mmol had the affirmative effect on the Cd uptake in the artificial Cd-contaminated soil.
Subject(s)
Cadmium/metabolism , Chelating Agents/metabolism , Environmental Restoration and Remediation/methods , Ricinus/metabolism , Soil Pollutants/metabolism , Biodegradation, Environmental , Citric Acid/metabolism , Edetic Acid/metabolism , Nitrilotriacetic Acid/metabolism , Quaternary Ammonium Compounds/metabolismABSTRACT
Gold surfaces functionalized with nickel-nitrilotriacetic acid (Ni²âº-NTA) as self-assembled monolayers (SAM) to immobilize histidine (His)-tagged biomolecules are broadly reported in the literature. However, the increasing demand of using microfluidic systems and biosensors takes more and more advantage on silicon technology which provides dedicated glass surfaces and substantially allows cost and resource savings. Here we present a novel method for the controlled oriented immobilization of His-tagged proteins on glass surfaces functionalized with a Ni²âº-NTA layer. Exemplarily, the protein pattern morphology after immobilization on the Ni²âº-NTA layer of His6-tagged soluble receptor for advanced glycation endproducts (sRAGE) was investigated and compared to non-oriented immobilization of sRAGE on amino SAM by using scanning electron microscopy (SEM). Moreover, we demonstrated interaction of immobilized sRAGE with three structurally different ligands, S100A12, S100A4, and glycated low density lipoproteins (glycLDL), by means of peak-force tapping atomic force microscopy (PF-AFM). We showed a clear discrimination of different protein-ligand orientations by differential height measurements.
Subject(s)
Histidine/genetics , Nitrilotriacetic Acid/analogs & derivatives , Oligopeptides/genetics , Organometallic Compounds/metabolism , Biosensing Techniques , Glass , Ligands , Nitrilotriacetic Acid/metabolism , S100 ProteinsABSTRACT
A bench scale system consisting of an up-flow packed bed bioreactor (UAPBR) made of polyurethane foam was used for the treatment and regeneration of aqueous solution of ferrous-NTA scrubbed with nitric oxide (NO). The biomass in the UAPBR was sequentially acclimatized under denitrifying and iron reducing conditions using ethanol as electron donor, after which nitric oxide (NO) gas was loaded continuously to the system by absorption. The system was investigated for different parameters viz. pH, removal efficiency of nitric oxide, biological reduction efficiency of Fe(II)NTA-NO and COD utilization. The Fe(II)NTA-NO reduction efficiency reached 87.8 % at a loading rate of 0.24 mmol L(-1) h(-1), while the scrubber efficiency reached more than 75 % with 250 ppm NO. Stover-Kincannon and a Plug-flow kinetic model based on Michaelis-Menten equation were used to describe the UAPBR performance with respect to Fe(II)NTA-NO and COD removal. The Stover-Kincannon model was found capable of describing the Fe(II)NTA-NO reduction (R m = 8.92 mM h(-1) and K NO = 11.46 mM h(-1)) while plug-flow model provided better fit to the COD utilization (U m = 66.62 mg L(-1) h(-1), K COD = 7.28 mg L(-1)). Analyses for pH, Fe(III)NTA, ammonium, nitrite concentration, and FTIR analysis of the medium samples indicated degradation of NTA, which leads to ammonium and nitrite accumulation in the medium, and affect the regeneration process.
Subject(s)
Bacteria/metabolism , Bioreactors/microbiology , Environmental Restoration and Remediation/methods , Ferrous Compounds/metabolism , Nitric Oxide/metabolism , Nitrilotriacetic Acid/metabolism , Anaerobiosis , Biodegradation, Environmental , Environmental Restoration and Remediation/instrumentation , Ferrous Compounds/chemistry , Kinetics , Nitric Oxide/chemistry , Nitrilotriacetic Acid/chemistryABSTRACT
To understand the metabolism of flavonoid rhamnoglycosides by human intestinal microbiota, we measured the metabolic activity of rutin and poncirin (distributed in many functional foods and herbal medicine) by 100 human stool specimens. The average α-Lrhamnosidase activities on the p-nitrophenyl-α-L-rhamnopyranoside, rutin, and poncirin subtrates were 0.10 ± 0.07, 0.25 ± 0.08, and 0.15 ± 0.09 pmol/min/mg, respectively. To investigate the enzymatic properties, α-L-rhamnosidase-producing bacteria were isolated from the specimens, and the α-L-rhamnosidase gene was cloned from a selected organism, Bifidobacterium dentium, and expressed in E. coli. The cloned α-L-rhamnosidase gene contained a 2,673 bp sequcence encoding 890 amino acid residues. The cloned gene was expressed using the pET 26b(+) vector in E. coli BL21, and the expressed enzyme was purified using Ni(2+)-NTA and Q-HP column chromatography. The specific activity of the purified α-L-rhamnosidase was 23.3 µmol/min/mg. Of the tested natural product constituents, the cloned α-L-rhamnosidase hydrolyzed rutin most potently, followed by poncirin, naringin, and ginsenoside Re. However, it was unable to hydrolyze quercitrin. This is the first report describing the cloning, expression, and characterization of α-L-rhamnosidase, a flavonoid rhamnoglycosidemetabolizing enzyme, from bifidobacteria. Based on these findings, the α-L-rhamnosidase of intestinal bacteria such as B. dentium seem to be more effective in hydrolyzing (1-->6) bonds than (1-->2) bonds of rhamnoglycosides, and may play an important role in the metabolism and pharmacological effect of rhamnoglycosides.
Subject(s)
Bifidobacterium/isolation & purification , Bifidobacterium/metabolism , Flavonoids/metabolism , Glycoside Hydrolases/genetics , Glycoside Hydrolases/metabolism , Intestines/microbiology , Microbiota , Rutin/metabolism , Base Sequence , Bifidobacterium/genetics , Cloning, Molecular , Cloning, Organism , Escherichia coli/genetics , Flavanones/metabolism , Ginsenosides/metabolism , Glycoside Hydrolases/chemistry , Humans , Molecular Sequence Data , Nitrilotriacetic Acid/analogs & derivatives , Nitrilotriacetic Acid/metabolism , Organometallic Compounds/metabolism , Quercetin/analogs & derivatives , Quercetin/metabolism , Rutin/biosynthesis , Sequence Alignment , Sequence Analysis, DNA , Substrate SpecificityABSTRACT
Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are related apicomplexan parasites that cause reproductive and neurological disorders in a wide range of domestic and wild animals. In the present study, the immunofluorescence antibody test (IFAT) was used to investigate the presence of antibodies against T. gondii, N. caninum and S. neurona in the sera of 11 free-living jaguars (Panthera onca) in two protected areas in the Pantanal region of Mato Grosso state, Brazil. Ten jaguars (90.9%) showed seropositivity for T. gondii, eight (72.7%) for S. neurona, and seven (63.6%) for N. caninum antigens. Our findings reveal exposure of jaguars to these related coccidian parasites and circulation of these pathogens in this wild ecosystem. To the best of our knowledge, this is the first serological detection of N. caninum and S. neurona in free-living jaguars.
Toxoplasma gondii, Neospora caninum e Sarcocystis neurona são coccídios relacionados responsáveis por causar desordens reprodutivas e neurológicas em uma ampla variedade de animais domésticos e selvagens. No presente estudo, a Reação de Imunofluorescência Indireta (RIFI) foi utilizada para investigar a presença de anticorpos contra T. gondii, N. caninum e S.neurona em soros de 11 onças-pintadas de vida livre (Panthera onca), provenientes de duas áreas protegidas na região do Pantanal do Estado de Mato Grosso, Brasil. Dez (90,9%), sete (63,6%) e oito (72,7%) onças amostradas foram soropositivas para T. gondii, N. caninum e S. neurona, respectivamente. Os resultados indicam a exposição a esses coccídios relacionados entre as onças-pintadas e a circulação ambiental desses patógenos nesse ecossistema selvagem. Este é o primeiro relato da detecção sorológica de N. caninum e S. neurona em onças-pintadas de vida livre.
Subject(s)
Humans , Iron/metabolism , Nitrilotriacetic Acid/analogs & derivatives , Biological Transport, Active , Chelating Agents , Ferric Compounds/metabolism , HeLa Cells , Kinetics , Nitrilotriacetic Acid/metabolism , Pentetic Acid , Transferrin/metabolismABSTRACT
BACKGROUND: Iron (Fe) deficiency anemia remains the largest nutritional deficiency disorder worldwide. How the gut acquires iron from nano Fe(III), especially at the apical surface, is incompletely understood. OBJECTIVE: We developed a novel Fe supplement consisting of nanoparticulate tartrate-modified Fe(III) poly oxo-hydroxide [here termed nano Fe(III)], which mimics the Fe oxide core of ferritin and effectively treats iron deficiency anemia in rats. METHODS: We determined transfer to the systemic circulation of nano Fe(III) in iron-deficient and iron-sufficient outbread Swiss mouse strain (CD1) mice with use of (59)Fe-labeled material. Iron deficiency was induced before starting the Fe-supplementation period through reduction of Fe concentrations in the rodent diet. A control group of iron-sufficient mice were fed a diet with adequate Fe concentrations throughout the study. Furthermore, we conducted a hemoglobin repletion study in which iron-deficient CD1 mice were fed for 7 d a diet supplemented with ferrous sulfate (FeSO4) or nano Fe(III). Finally, we further probed the mechanism of cellular acquisition of nano Fe(III) by assessing ferritin formation, as a measure of Fe uptake and utilization, in HuTu 80 duodenal cancer cells with targeted inhibition of divalent metal transporter 1 (DMT1) and duodenal cytochrome b (DCYTB) before exposure to the supplemented iron sources. Differences in gene expression were assessed by quantitative polymerase chain reaction. RESULTS: Absorption (means ± SEMs) of nano Fe(III) was significantly increased in iron-deficient mice (58 ± 19%) compared to iron-sufficient mice (18 ± 17%) (P = 0.0001). Supplementation of the diet with nano Fe(III) or FeSO4 significantly increased hemoglobin concentrations in iron-deficient mice (170 ± 20 g/L, P = 0.01 and 180 ± 20 g/L, P = 0.002, respectively). Hepatic hepcidin mRNA expression reflected the nonheme-iron concentrations of the liver and was also comparable for both nano Fe(III)- and FeSO4-supplemented groups, as were iron concentrations in the spleen and duodenum. Silencing of the solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2 (Slc11a2) gene (DMT1) significantly inhibited ferritin formation from FeSO4 (P = 0.005) but had no effect on uptake and utilization of nano Fe(III). Inhibiting DCYTB with an antibody also had no effect on uptake and utilization of nano Fe(III) but significantly inhibited ferritin formation from ferric nitrilotriacetate chelate (Fe-NTA) (P = 0.04). Similarly, cellular ferritin formation from nano Fe(III) was unaffected by the Fe(II) chelator ferrozine, which significantly inhibited uptake and utilization from FeSO4 (P = 0.009) and Fe-NTA (P = 0.005). CONCLUSIONS: Our data strongly support direct nano Fe(III) uptake by enterocytes as an efficient mechanism of dietary iron acquisition, which may complement the known Fe(II)/DMT1 uptake pathway.
Subject(s)
Duodenum/cytology , Duodenum/drug effects , Ferritins/administration & dosage , Nanoparticles/chemistry , Anemia, Iron-Deficiency/drug therapy , Animals , Cation Transport Proteins/genetics , Cation Transport Proteins/metabolism , Cell Line, Tumor , Dietary Supplements , Duodenum/metabolism , Enterocytes/metabolism , Ferric Compounds/metabolism , Ferritins/pharmacokinetics , Ferrous Compounds/administration & dosage , Ferrous Compounds/pharmacokinetics , Hemoglobins , Hepcidins/genetics , Hepcidins/metabolism , Iron, Dietary/administration & dosage , Iron, Dietary/pharmacokinetics , Liver/drug effects , Liver/metabolism , Male , Mice , Nitrilotriacetic Acid/analogs & derivatives , Nitrilotriacetic Acid/metabolism , Spleen/drug effects , Spleen/metabolism , Transcription Factors/genetics , Transcription Factors/metabolismABSTRACT
Desferrioxamine (DFO) is a potent iron chelator used in the treatment of iron overload (IO) disorders. However, due to its low cell permeability and fast clearance, DFO administration is usually prolonged and of limited use for the treatment of IO in tissues such as the brain. Caffeine is a safe, rapidly absorbable molecule that can be linked to other compounds to improve their cell permeability. In this work, we successfully prepared and described DFO-caffeine, a conjugate with iron scavenging ability, antioxidant properties and enhanced permeation in the HeLa cell model.
Subject(s)
Caffeine/pharmacology , Deferoxamine/pharmacology , Iron Chelating Agents/pharmacology , Iron Overload/drug therapy , Oxidative Stress/drug effects , Antioxidants/administration & dosage , Antioxidants/pharmacology , Caffeine/administration & dosage , Cell Membrane Permeability , Deferoxamine/administration & dosage , Drug Evaluation, Preclinical , Ferric Compounds/metabolism , Ferric Compounds/toxicity , Fluoresceins/analysis , Fluorescent Dyes/analysis , HeLa Cells , Humans , Iron Chelating Agents/administration & dosage , Molecular Structure , Nitrilotriacetic Acid/analogs & derivatives , Nitrilotriacetic Acid/metabolism , Nitrilotriacetic Acid/toxicityABSTRACT
Selective and fast labeling of proteins in living cells is a major challenge. Live-cell labeling techniques require high specificity, high labeling density, and cell permeability of the tagging molecule to target the protein of interest. Here we report on the site-specific, rapid, and efficient labeling of endogenous and recombinant histidine-tagged proteins in distinct subcellular compartments using cell-penetrating multivalent chelator carrier complexes. In vivo labeling was followed in real time in living cells, demonstrating a high specificity and high degree of colocalization in the crowded cellular environment.