ABSTRACT
The 70-amino-acid-residue N-terminal sequence of the bacterioferritin (BFR) of Azotobacter vinelandii was determined and shown to be highly similar to the N-terminal sequences of the Escherichia coli and Nitrobacter winogradskyi bacterioferritins. Electrophoretic and immunological analyses further indicate that the bacterioferritins of E. coli, A. vinelandii and Pseudomonas aeruginosa are closely related. A novel, two-subunit assembly state that predominates over the 24-subunit form of BFR at low pH was demonstrated. The results indicate that the bacterioferritins form a family of proteins that are distinct from the ferritins of plants and animals.
Subject(s)
Azotobacter/analysis , Bacterial Proteins/chemistry , Cytochrome b Group/chemistry , Escherichia coli/analysis , Ferritins/chemistry , Nitrobacter/analysis , Pseudomonas aeruginosa/analysis , Amino Acid Sequence , Bacterial Proteins/immunology , Chemical Phenomena , Chemistry, Physical , Cross Reactions , Cytochrome b Group/immunology , Electrophoresis, Polyacrylamide Gel , Ferritins/immunology , Immunodiffusion/methods , Isoelectric Focusing , Molecular Sequence Data , Species SpecificityABSTRACT
Carboxysomes were isolated from Nitrobacter winogradskyi and Nitrobacter agilis. The icosahedral particles contained double-stranded deoxyribonucleic acid (DNA). In the presence of ethidium bromide and cesium chloride, the particle-bound DNA had a buoyant density of rho 25 = 1.701 g/cm3. Electron microscopy revealed the DNA to be a 14-micron circular molecule.
Subject(s)
DNA, Bacterial/analysis , DNA, Circular/analysis , Inclusion Bodies/analysis , Nitrobacter/ultrastructure , Centrifugation, Density Gradient , Nitrobacter/analysisABSTRACT
Vaccenic acid (11-18:1) accounted for 92% of the fatty acids in the extractable lipids of log-phase Nitrobacter and Hyphomicrobium. During the stationary phase, both genera formed a 19-carbon cyclopropane fatty acid which increased in proportion to a decrease in the amount of vaccenic acid.
Subject(s)
Bacteria/analysis , Fatty Acids/analysis , Nitrobacter/analysis , Bacteria/growth & development , Bacteria/metabolism , Chloroform , Chromatography, Gas , Culture Media , Fatty Acids/metabolism , Fatty Acids, Essential/analysis , Lipids/analysis , Methanol , Nitrobacter/growth & development , Nitrobacter/metabolism , Solvents , Species Specificity , Time FactorsSubject(s)
Carbon Dioxide/metabolism , Nitrobacter/metabolism , Nitrosomonas/metabolism , Acetates/metabolism , Amino Acids/analysis , Amino Acids/biosynthesis , Aspartic Acid/metabolism , Carbon Isotopes , Carboxy-Lyases/metabolism , Chromatography, Paper , Citric Acid Cycle , Culture Media , Glutamates/metabolism , Ketoglutaric Acids/metabolism , Ligases/metabolism , Nitrobacter/analysis , Nitrobacter/enzymology , Nitrosomonas/analysis , Nitrosomonas/enzymology , Oxidoreductases/metabolism , Pyruvates/metabolism , Spectrophotometry , Succinates/metabolismABSTRACT
Fatty acids in the lipids of 19 marine and terrestrial nitrifying bacteria have been analyzed. Ammonia-oxidizing bacteria have a very simple acid composition; palmitic and palmitoleic acid account for 96 to 100% of the total acids. The fatty acids of nitrite-oxidizing bacteria cover a wider range, from C(14) to C(19), but from two to four acids still account for more than 80% of the total acids. Branched iso- and anteiso-acids are present in traces only in 2 of the 19 bacteria. The chemical and morphological similarity between blue-green algae and these bacteria is discussed.
