ABSTRACT
OBJECTIVE: The aim of this study is to characterize immunohistochemical profiles of lining epithelia of nasopalatine duct cyst (NPC) as well as to correlate those findings with their clinicopathological features to understand the histopathogenesis of NPC. MATERIALS AND METHODS: Forty-one surgical specimens from NPC were examined for clinical profiles and expression of keratin-7, 13, MUC-1, and P63 by immunohistochemistry, compared to radicular cyst (RC) and maxillary sinusitis. RESULTS: Nasopalatine duct cyst was clinically characterized by male predominant occurrence: 44% of the cases involved tooth roots, and 70% with inflammatory backgrounds. Lining epithelia of NPCs without daughter cysts were immunohistochemically distinguished into three layers: a keratin 7-positive (+) ciliated cell layer in the surface, a keratin-13+ middle layer, and a MUC-1+/P63+ lower half, indicating that they were not respiratory epithelia, and the same layering pattern was observed in RC. However, those immunolocalization patterns of the main cyst lining with daughter cyst were exactly the same as those of daughter cyst linings as well as duct epithelia of mucous glands. CONCLUSIONS: Two possible histopathogenesis of NPC were clarified: one was inflammatory cyst like RC and the other was salivary duct cyst-like mucocele.
Subject(s)
Maxillary Diseases/etiology , Nasal Cavity/pathology , Nonodontogenic Cysts/etiology , Nonodontogenic Cysts/pathology , Palate, Hard/pathology , Adult , Aged , Epithelial Cells/pathology , Female , Humans , Inflammation/complications , Keratins/metabolism , Male , Maxillary Diseases/metabolism , Maxillary Diseases/pathology , Maxillary Sinusitis/pathology , Membrane Proteins/metabolism , Middle Aged , Mucins/metabolism , Mucocele/complications , Nonodontogenic Cysts/metabolism , Radicular Cyst/pathology , Sex Ratio , Terminology as Topic , Tooth Root/pathology , Young AdultABSTRACT
BACKGROUND: Cytokeratin (CK) expression patterns have been studied in numerous intact and diseased oral tissues. However, CK expression in metaplastic squamous cells has not been explored in depth and the origin of metaplastic epithelial linings of the jaw cysts has not been sufficiently investigated. METHODS: We examined CK expression in 46 postoperative maxillary cysts (POMCs) which were lined with pseudostratified columnar cells only, columnar and squamous cells, and squamous cells only, in 13, 30 and 3 cases, respectively. RESULTS: The expression of CK8, CK13 and CK18 were observed in 39, 9 and all 43 of the columnar epithelial linings, respectively. Metaplastic squamous epithelia expressed more CK13, and less CK18 and CK8. Of the 33 metaplastic linings, 24 expressed CK8, 23 CK13 and 26 linings expressed CK18. The patterns of expression of CK13 and CK18 observed were CK18(+)-CK13(-) in 10 metaplastic linings, CK18(+)-CK13(+) in 16, and CK18(-)-CK13(+) in 7. The expression of CK13- and CK18-mRNA was generally correlated with level of protein expressed. CK18-mRNA expression was observed by in situ hybridization, not only in the 26 metaplastic linings which were positive for CK18 protein, but also in five of the seven metaplastic linings which did not express CK18 protein. In addition, RT-PCR revealed an expression of CK18-mRNA in all metaplastic squamous linings, although the expression level was weaker than that in the columnar epithelial linings. The CK13-mRNA was expressed inversely to the CK18-mRNA. CONCLUSIONS: These results indicate that CK18-mRNA is preserved through metaplasia, although the protein expression decreased. Metaplastic squamous cells differentiate with a decrease of CK18 and an increase of CK13 expression.
Subject(s)
Cell Differentiation/physiology , Keratins/biosynthesis , Maxillary Diseases/metabolism , Nonodontogenic Cysts/metabolism , Nonodontogenic Cysts/pathology , Cell Differentiation/genetics , Epithelial Cells/metabolism , Epithelial Cells/pathology , Gene Expression , Humans , Immunohistochemistry , In Situ Hybridization , Isoelectric Focusing , Maxillary Diseases/pathology , Metaplasia/metabolism , Metaplasia/pathology , RNA, Messenger/analysis , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
In a review of 20 nasopalatine duct cysts, two cases were encountered with a pigmented epithelial lining. In the literature we have found one similar case only. The pigment in our cases was shown to be melanin. The authors suggest that the melanin-containing epithelial cells are derived from Jacobson's organs and, therefore, most likely should be considered olfactory epithelium.
Subject(s)
Mouth Diseases/pathology , Nonodontogenic Cysts/pathology , Nose Diseases/pathology , Palate/pathology , Adult , Humans , Male , Melanins/metabolism , Middle Aged , Mouth Diseases/metabolism , Nonodontogenic Cysts/metabolism , Nose Diseases/metabolism , Palate/metabolismABSTRACT
Jaw cyst lining cells have an active transporting mechanism for Na+ ion and K+ion, a secreting mechanism and a selecting mechanism, and they allow permeation of electrolytes, lipids and protein into cysts. The components within the cysts have a controlling metabolism, and keep the system stable. Tumour wall cells of cystic ameloblastoma have only a passive transporting mechanism for various substances. Their nature differs from that of jaw cyst lining cells.
