ABSTRACT
The authors describe and summarise the use of nucleic acid hybridisation and polymerase chain reaction (PCR) technologies in the diagnosis of animal diseases. PCR is a powerful biological tool which enables exponential enzymatic amplification in vitro of a given deoxyribonucleic acid sequence. This technique is currently used to study the molecular pathogenesis of many infectious diseases and also for diagnosis. PCR is usually more sensitive than conventional methods and does not require complex facilities, and will therefore soon become the preferred technology of laboratory diagnosticians, field veterinarians and health officers.
Subject(s)
Communicable Diseases/veterinary , DNA/analysis , Nucleic Acid Hybridization/veterinary , Polymerase Chain Reaction/veterinary , RNA/analysis , Animals , Bacterial Infections/diagnosis , Bacterial Infections/veterinary , Communicable Diseases/diagnosis , DNA/isolation & purification , RNA/isolation & purification , Virus Diseases/diagnosis , Virus Diseases/veterinaryABSTRACT
Various methods have been employed for the diagnosis of pseudorabies in Argentina. A large serological survey was carried out by means of enzyme-linked immunosorbent assay (blocking ELISA) and virus neutralisation (VN). An outbreak was studied by virological and immunohistochemical methods and in situ nucleic acid hybridisation.