ABSTRACT
PURPOSE: To investigate whether unilateral in vivo UVR-B exposure of one eye affects the fellow eye in a co-cataractogenic, sympathetic reaction and to determine whether an inflammatory response could be involved in the pathogenesis. METHODS: C57BL/6 mice were unilaterally exposed in vivo to UVR-B for 15 min. In the group of 24 animals each received 0×/2×/3×/or 4× cataract threshold equivalent dose. Following 48-hr UVR-B exposure, cataract morphology was documented in dark-field illumination photography, and light scattering was quantified, in both lenses in vitro. Serum levels of pro-inflammatory cytokines IL-1ß, IL-6 and TNF-α were analysed with ELISA. Immunohistochemistry was performed for inflammatory infiltration in exposed and contralateral eyes. RESULTS: UVR-B exposure induced cataract in all exposed lenses. There was additionally a significant UVR dose-dependent increase in light scattering in the lenses of the non-exposed fellow eye. Inflammatory infiltration was detected immunohistochemically in the anterior segment of both eyes. IL-1ß serum concentration increased with increasing UVR-B exposure dose. There was a similar trend for serum IL-6 but not for TNF-α. CONCLUSION: Unilateral UVR-B exposure to one eye is associated with intraocular inflammation and an increase in lens light scattering also in the unexposed, fellow eye. A resulting systemic inflammatory response might be mediated by IL-1ß and possibly IL-6. The finding that an inflammatory response may play a role in UVR-B-induced cataract development might initiate new strategies in the prevention of the disease.
Subject(s)
Cataract/etiology , Lens, Crystalline/radiation effects , Ophthalmia, Sympathetic/etiology , Radiation Injuries, Experimental/etiology , Ultraviolet Rays/adverse effects , Animals , Anterior Eye Segment/pathology , Cataract/blood , Cataract/pathology , Dose-Response Relationship, Radiation , Enzyme-Linked Immunosorbent Assay , Female , Interleukin-1beta/blood , Interleukin-6/blood , Lens, Crystalline/pathology , Light , Macrophages/pathology , Mice , Mice, Inbred C57BL , Monocytes/pathology , Neutrophils/pathology , Ophthalmia, Sympathetic/blood , Ophthalmia, Sympathetic/pathology , Radiation Injuries, Experimental/blood , Radiation Injuries, Experimental/pathology , Scattering, Radiation , Tumor Necrosis Factor-alpha/bloodABSTRACT
Serum sialic acid levels were measured in 16 patients with sympathetic ophthalmitis, 36 with neglected traumatic uveitis following penetrating injury and 40 healthy subjects. There was no significant alteration of its level in patients with traumatic uveitis. However, its level was significantly elevated in patients with sympathetic ophthalmitis. It was high even in the early stage of the disease. It decreased significantly at the remission stage. It is proposed that measurement of sialic acid level in serum can be used as a diagnostic aid when the diagnosis of sympathetic ophthalmitis remains doubtful on clinical grounds. The extent of rise in its level may be considered a good parameter of the degree of severity of sympathetic ophthalmitis. It may also act as a useful tool to evaluate the drug efficacy in this disease.
