ABSTRACT
Standard cytophotometric measurement of DNA in normal, leukoplakic and cancerous oral and oropharyngeal tissues with a Leitz Weitzler Aristophot Cytophotometer showed both 1% and 5% significance in different grades of malignancy and 5% as regards sites of malignancy. The differences were marked in different grades of malignancy and specially with progression of the lesion. Cytophotometry can be useful to diagnose the stages of carcinoma.
Subject(s)
DNA, Neoplasm/analysis , Mouth Neoplasms/analysis , Adult , Biomarkers, Tumor/analysis , DNA/analysis , Female , Humans , Leukoplakia, Oral/analysis , Leukoplakia, Oral/diagnosis , Male , Middle Aged , Mouth/analysis , Mouth Neoplasms/diagnosis , Mouth Neoplasms/pathology , Oropharyngeal Neoplasms/analysis , Oropharyngeal Neoplasms/diagnosis , Oropharyngeal Neoplasms/pathology , Oropharynx/analysis , Reference ValuesABSTRACT
Cyclic nucleotides (cAMP, cGMP) are suggested to participate in the regulation of cell growth and differentiation. Guanylate cyclase is the enzyme which catalyzes the synthesis of cGMP. The basal guanylate cyclase activity was slightly higher in well-differentiated squamous cell carcinomas than in normal mucosa, but was two-fold higher in papillomas. Poorly differentiated squamous cell carcinoma did not show any increased basal activity. Stimulation with nitroprusside (NP) resulted in a 20% increased activity for normal mucosa and a 30% increase for poorly differentiated carcinomas, whereas enzyme prepared from well-differentiated squamous carcinomas and papillomas showed a two-fold increase.
Subject(s)
Carcinoma, Squamous Cell/enzymology , Guanylate Cyclase/metabolism , Laryngeal Neoplasms/enzymology , Mouth Neoplasms/enzymology , Oropharyngeal Neoplasms/enzymology , Papilloma/enzymology , Pharyngeal Neoplasms/enzymology , Carcinoma, Squamous Cell/analysis , Epithelium/enzymology , Guanylate Cyclase/analysis , Humans , Laryngeal Neoplasms/analysis , Mouth Neoplasms/analysis , Oropharyngeal Neoplasms/analysis , Papilloma/analysisABSTRACT
The existence of hormone receptors on or within neoplastic tissue has potential diagnostic, therapeutic, and prognostic importance. It was the purpose of this study to measure estrogen and progesterone receptors in a large group of patients with head and neck cancer to determine their frequency. Sixty-five patients with head and neck tumors underwent a total of 75 estrogen and 50 progesterone receptor assays. In this group, 87.7% were squamous cell carcinoma. In the estrogen receptor assays, 89.3% (67/75) were negative, 8% (6/75) were borderline, and only 2.7% (2/75) were positive. In the progesterone receptor assays, 78% (39/50) were negative, 22% (11/50) were borderline, and there were no positive results. There were no changes in assays of tissue removed at biopsy v tissue removed during surgery. There was no impact with chemotherapy. In conclusion, head and neck cancers do not appear to possess estrogen or progesterone receptors and can be considered to be hormonally independent.
