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Arch Virol ; 157(4): 773-6, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22227800

ABSTRACT

In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-throughput sequencing. The full genome of a new potyvirus could be assembled from the resulting siRNA sequences, and it was sufficiently different from other sequences to be considered a member of a new species, which we have designated Yam bean mosaic virus (YBMV). Sequence similarity suggests that YBMV has also been detected in yam beans in Indonesia.


Subject(s)
Genome, Viral , Pachyrhizus/virology , Plant Diseases/virology , Potyvirus/genetics , Potyvirus/isolation & purification , RNA, Viral/genetics , Cluster Analysis , Molecular Sequence Data , Peru , Phylogeny , RNA, Small Interfering/genetics , Sequence Analysis, DNA , Sequence Homology
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