The distribution of presumptive thoracic paraganglionic tissue in the common marmoset (Callithrix jacchus).

The aortic-pulmonary regions (APR) of seven adult marmosets (Callithrix jacchus) and the region of the right subclavian artery of a further three marmosets were diffusion-fixed with 10% buffered formol-saline solution. In both regions serial 5 microm sections were cut and stained by the Martius yellow, brilliant crystal scarlet and soluble blue method. Presumptive thoracic paraganglionic (PTP) tissue was only observed in the APR. PTP tissue was composed of small groups of cells that varied in size and number. The distribution of the groups of cells was extremely variable, so much so that it would be misleading to attempt to classify their position; they were not circumscribed by a connective tissue capsule, but were always related to the thoracic branches of the left vagus nerve. The cells lay in loose areolar tissue characteristic of this part of the mediastinum and received their blood supply from small adjacent connective tissue arterioles. Unlike the paraganglionic tissue found in the carotid body the cells in the thorax did not appear to have a profuse capillary blood supply. There was, however, a close cellular-neural relationship. The cells, 10-15 microm in diameter, were oval or rounded in appearance and possessed a central nucleus and clear cytoplasm. No evidence was found that these cells possessed a 'companion' cell reminiscent of the arrangement of type 1 and type 2 cells in the carotid body. In conclusion, we found evidence of presumed paraganglionic tissue in the APR of the marmoset which, however, did not show the characteristic histological features of the aortic body chemoreceptors that have been described in some non-primate mammals. A survey of the mediastina of other non-human primates is required to establish whether this finding is atypical for these animals.

Chemosensitivity, plasticity, and functional heterogeneity of paraganglionic cells in the rat coeliac-superior mesenteric complex.

Chemosensitivity and plasticity of paraganglionic cells in the rat coeliac-superior mesenteric complex (CSMC) were investigated at a basal state of normoxia (21% O2) and after long-term moderate hypoxia (10% O2, 14 days). Chemical sympathectomy previous to hypoxia was performed to destroy principal ganglionic neurons and thus to allow measurement of the norepinephrine and dopamine content of paraganglionic cells. At the basal state, the CSMC contained dopaminergic (TH+/DBH-) and noradrenergic (TH+/DBH+) paraganglionic cells, the majority being of the noradrenergic type. After 14 days of hypoxia, this ratio was reversed and dopaminergic cells predominated, as indicated by a twofold increase of TH+ cells and a twofold decrease of DBH+ cells. Biochemically, hypoxia produced an increase in the content (1.6-fold) and utilization (1.4-fold) of dopamine as well as a smaller increase in the content of norepinephrine, with no change in its utilization rate. The dopaminergic activation induced by hypoxia persisted after sympathectomy with guanethidine. It is concluded that paraganglionic cells in the CSMC display a chemosensitive function. Furthermore, our findings indicate that paraganglionic cells are differentially affected by hypoxia, depending on their distribution and the nature of their neuromodulators. The alterations induced by hypoxia point out the phenotypic plasticity developed by paraganglionic cells in adaptation to hypoxia and further demonstrate the functional heterogeneity of this autonomic cell population in the rat CSMC.

Immunohistochemistry of small intensely fluorescent (SIF) cells and of SIF cell-associated nerve fibers in the rat superior cervical ganglion.

Double-labelling immunofluorescence was applied on single sections of the rat superior cervical ganglion to evaluate neurochemistry and connectivity of intraganglionic SIF cells. The synaptic vesicle membrane protein synaptophysin and secretoneurin, a newly discovered neuropeptide derived from secretogranin II, proved reliable molecular markers of this cell type, whereas serotonin and tyrosine hydroxylase immunoreactivities were observed in slightly incongruent SIF cell subpopulations. Immunolabelling for vasoactive intestinal polypeptide and neuropeptide Y occurred in few SIF cells. None of the above immunoreactivities were visibly altered by preganglionic or postganglionic denervation, while some SIF cells were immunolabelled for galanin or for the neuronal microtubule-associated protein MAP2 after postganglionic denervation. SIF cells were nonreactive for the pan-neuronal marker protein gene product (PGP) 9.5 or neurofilament 160 kD. Intense staining of NADPH-diaphorase in some SIF cells, suggesting catalytic activity of nitric oxide synthase, could not be substantiated by immunoreactivity for this enzyme. SIF cells were approached by nonidentical fiber populations immunoreactive for PGP 9.5, neurofilament, or neuropeptide Y, whereas immunoreactivities for galanin and vasoactive intestinal polypeptide were colocalized in fiber meshes around SIF cells. The findings indicate (1) neurochemical SIF cell heterogeneity, (2) SIF cell plasticity in response to ganglionic perturbation, and (3) a differentiated innervation of SIF cells in the rat superior cervical ganglion.

Serotonin immunoreactivity in the autonomic intrapulmonary ganglia of the fetal sheep.

Using light microscopic immunohistochemistry, serotonin immunoreactivity was found in paraganglionic cells within pulmonary autonomic ganglia of fetal sheep at pseudoglandular and canalicular stages of lung development. Serotonin immunoreactive cells appeared individually or in clusters, and were occasionally seen around or in close contact with blood vessels.

Peptidergic innervation of arterial chemoreceptors.

The peptidergic innervation of arterial chemoreceptor organs (the rat carotid body and vagal paraganglia; guinea pig carotid body) was studied immunohistochemically. Five different populations of nerve fibres in the guinea pig carotid body could be discriminated according to their origin and their chemical coding. The innervation pattern of the rat carotid body differed in some aspects. Comparison of the rat carotid body and vagal paraganglia suggested that autonomic neuropeptide Y-like immunoreactive fibres act primarily via vascular mechanisms rather than directly on the chemoreceptor tissue. Sensory fibres were shown to contain immunoreactivities for substance P, calcitonin gene-related peptide (rat and guinea pig) and somatostatin (guinea pig). The functional role of the identified peptide-containing sensory fibres remains to be established.

Postnatal development of the innervation and paraganglia in the porcine pulmonary arterial bed.

The innervation of the pulmonary trunk and pulmonary arterial bed was studied in 17 pigs from birth to 6 months of age. After birth, the pulmonary trunk and extra- and intra-pulmonary arteries contained neurofilament and protein gene-product-immunoreactive nerve fibres in both the adventitia and media. The density of nerve fibres increased from birth to 2 months, this being most marked during the first 2 weeks of life. Most of the fibres in the media were presumed to be sympathetic in origin as they contained both neuropeptide tyrosine and tyrosine hydroxylase immunoreactivity. Fibres were associated with the vasa-vasorum and vascular smooth muscle running around the vessel, between the elastic laminae and smooth muscle cells, in the outer two-thirds of the media. Vasoactive intestinal polypeptide and calcitonin gene-related peptide-immunoreactive nerve fibres were found to be associated with the pulmonary trunk and extra-pulmonary artery, but generally not with the intra-pulmonary arteries. Tyrosine hydroxylase immunoreactivity was detected in the glomus cells at birth, but peptide immunoreactivity (enkephalin) was not demonstrated in paraganglia until 14 days of age. Adaptation to extra-uterine life is associated with rapid development changes in the innervation of the pig pulmonary trunk, extra- and intra-pulmonary arteries and in the expression of peptide immunoreactivity in both nerve fibres and glomus cells. These changes may have a role in the postnatal adaptation of the pulmonary circulation.

Paraganglia of the human recurrent laryngeal nerve.

Recurrent laryngeal nerves (RLNs) from human autopsy material were collected and prepared for light microscopy. Within five of eight investigated RLNs, paraganglia-like organs were found, the mean cross-sectional area of which was estimated to be 0.03 mm2. These organs were composed of cells resembling the type I and type II cells of the carotid body. The possible function of laryngeal nerve paraganglia is discussed.

[Chemodectoma of nerve X. Value and interest of the concept of the diffuse paraganglionic system]. / Chemodectomes du X. Valeur et intérêt du concept de système para-ganglionnaire diffus.

Tenth nerve chemodectoma is one of the rarest varieties of cervicocephalic paragangliomas, far behind tumors developed from the carotid body. Based on findings in three patients, one reported previously, clinical and biological features of these formations are discussed. Recent data and those from personal studies are exposed, and arguments presented enabling the concept to be defended of a diffuse paraganglionic system in relation with the vagus nerve.

Location and size of carotid body-like organs (paraganglia) revealed in rats by the permeability of blood vessels to Evans blue dye.

We determined the number, distribution size, and morphology of paraganglia near the glossopharyngeal, vagus, and sympathetic nerves of rats. The location of paraganglia was revealed by a method that takes advantage of the comparatively high permeability of their blood vessels to Evans blue dye. Rats were fixed by vascular perfusion of glutaraldehyde 2 min after receiving an intravenous injection of Evans blue dye. Paraganglia appeared as circumscribed, intensely blue structures that were readily distinguished from unstained nerves associated with them. Similarly, some groups of small intensely fluorescent (SIF) cells in autonomic and sensory ganglia were surrounded by Evans blue at a time that other portions of the ganglia contained little detectable dye. An average of 92.5 (range 41-134) paraganglia and 41 (range 17-68) blue spots in ganglia were found in the neck, thorax and abdomen of each of 10 rats. Carotid bodies had a mean length of 601 +/- 123 micrometer, width of 275 +/- 65 micrometer, and volume of 25.1 +/- 11.2 micrometer 3 X 10(6). Other paraganglia had an average length of 168 +/- 108 micrometer, width of 77 +/- 41 micrometer, and volume of 0.87 +/- 1.55 micrometer 3 X 10(6). The total volume of paraganglion tissue averaged 128 micrometer 3 X 10(6) (range 62-215 micrometer 3 X 10(6)), 59% of which was due to paraganglia other than the carotid bodies. By using fluorescence microscopy, we verified that small catecholamine-containing cells, visible because of their yellow-green fluorescence induced by formaldehyde gas, were located in regions along nerves and within ganglia that contained extravascular dye, visible because of its red fluorescence. Electron-microscopic studies confirmed that blue-stained organs (presumptive paraganglia) associated with the superior laryngeal nerve and other branches of the vagus nerve contained cells morphologically similar to glomus cells of the carotid body. Celiac ganglia contained, in addition, some cells similar to chromaffin cells of the adrenal medulla. Paraganglia (but not in SIF cells in ganglia) were encapsulated by layers of perineurium, which may constitute a barrier to diffusion. Tortuous thin-walled blood vessels, some with a fenestrated endothelium, were present in all paraganglia examined and were near most groups of SIF cells in ganglia. Neural connections of the small catecholamine-containing cells varied. Most nerve terminals on cells in paraganglia resembled sensory nerve endings on glomus cells of the carotid body, although some were morphologically similar to preganglionic nerves on chromaffin cells of the adrenal medulla.

Paraganglia in the human gallbladder.

Paraganglia found in the subserosa of nine of ten surgically removed gallbladders had morphologic features and staining characteristics similar to those of other sites. Although they were not difficult to locate in subserial sections, their sparseness and smallness explains the rarity with which they have been encountered in the routine histologic gallbladder examination. The strong, finely granular argyrophilia of the chief cell cytoplasm in all of the glomera studied offer a simple means of identifying them when only a portion of the structure is viewed.

Efferent fibres to the aortic bodies: a search for their cell bodies in the brainstem of the cat and rabbit.

An attempt has been made to determine where in the lower brainstem the cell bodies of nonsympathetic efferent fibres in the aortic nerve of the cat and rabbit are located. Horseradish peroxidase (HRP) was placed on the central end of the right cut aortic nerve of anaesthetized animals and, after an appropriate time, sections of the brainstem encompassing the rostral and caudal limits of the dorsal vagal motor nucleus and nucleus ambiguus were examined microscopically for retrogradely transported HRP. Cell bodies labelled by exogenous HRP were not found in any of the cats or rabbits exposed to HRP although reaction product, due to an endogenous response, was observed. Appropriate control experiments were performed to show that the sensitivity of the technique for demonstrating HRP in our hand was adequate. We conclude that the cell bodies of efferent fibres, of non sympathetic origin, in the aortic nerve are likely to be located outside the central nervous system.

Sympathetic innervation of the pulmonary artery, ascending aorta, and coronar glomera of the rabbit. A fluorescence microscopic study.

Adrenergic nerve fibres were demonstrated in the connective tissue of the rabbit coronar glomera by means of the formaldehyde-induced fluorescence technique for catecholamines. This type of innervation is similar to the adrenergic nerve supply to the rabbit and cat carotid body. Adrenergic fibres terminate subendothelially and only a few can be traced to type I cells in the glomera coronaria. The sympathetic innervation of the ascending aorta is exceedingly sparse in contrast to the pulmonary trunk, while vasa vasorum of the ascending aorta exhibit a dense sympathetic innervation.

Chemoreceptor properties of glomus tissue found in the carotid region of the cat.

1. ;Miniglomera' appearing as small masses of tissue with ample vascularization were found around the common carotid artery of the cat. Physiological, gross anatomical and electron microscopic studies were conducted on these tissues.2. The chemosensory function of each ;miniglomus' was evident from the behaviour of the afferent nerve fibres supplying the tissue: afferent responses became more active during asphyxia, when the blood flow through the tissue was reduced or blocked and when cyanide or ACh were applied. The afferent impulses became more infrequent during hyperventilation.3. Sensory frequency response curves constructed against percentage of inhaled O(2) showed that the impulses of single units increased in frequency with lowering of O(2) content of the inhaled gas.4. These miniglomera are innervated by afferent fibres emerging from the nodose ganglion; sometimes these fibres are contained in the aortic or common carotid baroreceptor nerves, but sometimes they emerge as independent nerves. None of the miniglomera are supplied by branches of the sinus nerve.5. The fine structure of the miniglomus is similar to that of the carotid body. The tissue contains two types of cells: glomus cells which contain dense cored granules, and sustentacular cells whose fine processes enclose the former. Membrane densifications occur where glomus cells lie adjacent to one another or where they are contacted by nerve terminals. Nerve fibres are common in the miniglomus but they contact glomus cells less frequently than in the carotid body.