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1.
J Eukaryot Microbiol ; 54(1): 1-7, 2007.
Article in English | MEDLINE | ID: mdl-17300509

ABSTRACT

In the past decades, the major focus of antigen variation research has been on parasitic protists. However, antigenic variation occurs also in free-living protists. The antigenic systems of the ciliates Paramecium and Tetrahymena have been studied for more than 100 yr. In spite of different life strategies and distant phylogenetic relationships of free-living ciliates and parasitic protists, their antigenic systems have features in common, such as the presence of repeated protein motifs and multigene families. The function of variable surface antigens in free-living ciliates is still unknown. Up to now no detailed monitoring of antigen expression in free-living ciliates in natural habitats has been performed. Unlike stochastic switching in parasites, antigen expression in ciliates can be directed, e.g. by temperature, which holds great advantages for research on the expression mechanism. Regulated expression of surface antigens occurs in an exclusive way and the responsible mechanism is complex, involving both transcriptional and post-transcriptional features. The involvement of homology-dependent effects has been proposed several times but has not been proved yet.


Subject(s)
Antigenic Variation , Antigens, Protozoan/immunology , Paramecium/immunology , Tetrahymena/immunology , Animals , Antigenic Variation/genetics , Antigens, Protozoan/chemistry , Antigens, Protozoan/genetics , Antigens, Surface/genetics , Antigens, Surface/immunology , Gene Expression Regulation , Genome, Protozoan , Paramecium/classification , Paramecium/genetics , Serotyping , Signal Transduction , Tetrahymena/genetics
2.
Protoplasma ; 225(3-4): 157-67, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16228897

ABSTRACT

Many of the highly organized microtubular arrangements in ciliates are located in the cortical area containing membrane vesicles and vacuoles. In Tetrahymena thermophila and Paramecium caudatum, immunofluorescence microscopy with the monoclonal antibody TU-06, directed against beta-tubulin, revealed distinct staining of this cortical region alone, while the cilia and other microtubular structures were unstained. The specificity of the antibody was confirmed by immunoblotting and by preabsorption of the antibody with purified tubulin. Double-label immunofluorescence with antibodies against gamma-tubulin, detyrosinated alpha-tubulin, and centrin showed that the TU-06 epitope is localized outside the basal body region. This was also confirmed by immunogold electron microscopy of thin sections. Proteolytic digestion of porcine brain beta-tubulin combined with a peptide scan of immobilized, overlapping peptides disclosed that the epitope was in the beta-tubulin region beta81-95, a region which is phylogenetically highly conserved. As known posttranslational modifications of beta-tubulin are located outside this area, the observed staining pattern cannot be interpreted as evidence of subcellular sequestration of modified tubulin. The limited distribution of the epitope could rather reflect the dependence of TU-06 epitope exposition on conformations of tubulin molecules in microtubule arrangements or on differential masking by interacting proteins.


Subject(s)
Epitopes/analysis , Paramecium/immunology , Tetrahymena thermophila/immunology , Tubulin/immunology , 3T3 Cells , Animals , Cell Membrane/immunology , Epitope Mapping , Epitopes/immunology , Epitopes/metabolism , Immunoblotting , Mice
3.
J Eukaryot Microbiol ; 52(4): 319-27, 2005.
Article in English | MEDLINE | ID: mdl-16014009

ABSTRACT

Paramecium primaurelia expresses three major types of surface antigens. We report here the identification of the gene for serotype S, which completes the sequence data of expressed serotypes of P. primaurelia. The complete open reading frame of surface antigen S was identified using a novel technique, based upon the presence of conservative regions in the non-coding areas of the multigene family. We were able to isolate the 7194-bp-long open reading frame from the macronuclear DNA for Serotype 156S. The corresponding mRNA was detected in the two serotype S-expressing stocks, 60 and 156, of P. primaurelia, which clarifies that both stocks are using the same S allele. Comparisons of the nucleic acid and the deduced amino-acid sequence showed high identity to surface antigen 51B of P. tetraurelia, sufficient to cause an immunological cross-reaction in vivo. Immunologically relevant epitopes in vivo were identified in the central regions of the genes, constructed of nearly perfect tandem repeats.


Subject(s)
Antigens, Protozoan/genetics , Antigens, Protozoan/immunology , Paramecium/genetics , Paramecium/immunology , Amino Acid Sequence , Animals , Antigenic Variation/genetics , Antigenic Variation/immunology , Antigens, Surface/genetics , Antigens, Surface/immunology , Base Sequence , DNA, Protozoan/chemistry , DNA, Protozoan/genetics , Epitopes/genetics , Epitopes/immunology , Molecular Sequence Data , RNA, Protozoan/chemistry , RNA, Protozoan/genetics , Reverse Transcriptase Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNA , Surface Properties
4.
FEMS Microbiol Lett ; 238(2): 449-53, 2004 Sep 15.
Article in English | MEDLINE | ID: mdl-15358432

ABSTRACT

The presence of opioid, beta-adrenergic and cholinergic receptors has been demonstrated in ciliated protozoa, but little is known about gamma-aminobutyric acid (GABA) receptors. In this study we have analyzed the distribution of GABA(A)-type receptor subunits in Paramecium. Confocal laser microscopy using antibodies specific for alpha(1)-, alpha(2)-, alpha(3)-, alpha(6)-, beta(2/3)-, gamma(2)-, epsilon-, lambda-, and theta-subunits showed that most receptors are aggregated in clusters and are distributed both on cell surface and in the cytoplasm. The intensity of labelling of the alpha(6)-, beta(2/3)- and gamma(2)-subunits was more intense than the alpha(1)-, epsilon-, and theta-subunits, suggesting that the former are present in higher concentrations than the latter.


Subject(s)
Paramecium/chemistry , Receptors, GABA-A/analysis , Animals , Antibody Specificity , Fluorescent Antibody Technique , Microscopy, Confocal/methods , Paramecium/immunology , Receptors, GABA-A/immunology
5.
J Membr Biol ; 200(1): 15-23, 2004 Jul 01.
Article in English | MEDLINE | ID: mdl-15386156

ABSTRACT

We analyzed temperature-induced changes of variant surface antigen (vsAg) expression in Paramecium primaurelia, using immuno-techniques and mRNA determinations. Upon a 23 degrees C to 33 degrees C shift, the old vsAg, type 156G, remains on the cell surface for a time, when already mRNA for the new form, 156D, is expressed. A considerable amount of 156D-specific mRNA is formed 45-48 h after the temperature shift, while 156D surface expression reaches maximal levels only after >72 h. A new aspect of these experiments is that, during this transition, the old vsAg is steadily released in high-molecular-weight form into the culture medium, as found by dot blot and Western blot analysis of concentrated culture medium. The new vsAg form is first inserted into the somatic cell membrane, before it spreads also into cilia. In the reverse transition, 33 degrees C to 23 degrees C, the adaptation on the level of transcription and surface expression is considerably faster. While we had previously shown, under steady-state conditions (constant temperature), the occurrence of a degradation pathway by endocytotic and phagocytotic ingestion of vsAg this may proceed in parallel to the steady release of old vsAg from the cell surface into the medium. Altogether these combined processes may facilitate the installation of the new vsAg type.


Subject(s)
Antigens, Protozoan/immunology , Antigens, Surface/immunology , Paramecium/immunology , Temperature , Transcription, Genetic , Animals , Cilia/immunology , Culture Media/chemistry , Microscopy, Immunoelectron , Protozoan Proteins/immunology
6.
Histochem J ; 34(1-2): 51-6, 2002.
Article in English | MEDLINE | ID: mdl-12365800

ABSTRACT

Cell fractionation, SDS-PAGE, quantitative Western blot, confocal immunolocalization and immunogold labelling were performed to find an interpretation of the physiological response of the unicellular eukaryote Paramecium to beta-adrenergic ligands. The 69 kDa polypeptide separated by SDS-PAGE in S2 and P2 Paramecium subcellular fractions cross-reacted with antibody directed against human beta2-adrenergic receptor. This was detected by Western blotting followed by chemiluminescent detection. Quantitative image analysis showed that beta-selective adrenergic agonist (-)-isoproterenol--previously shown to enhance phagocytic activity--evoked redistribution of the adrenergic receptor analogue from membraneous (P2) to cytosolic (S2) fraction. The relative increase in immunoreactive band intensity in S2 reached 80% and was paralleled by a 59% decrease in P2 fraction. Confocal immunofluorescence revealed beta2-adrenergic receptor sites on the cell surface and at the ridge of the cytopharynx--where nascent phagosomes are formed. This localization was confirmed by immunoelectron microscopy. These results indicate that the 69 kDa Paramecium polypeptide immunorelated to vertebrate beta2-adrenergic receptor appeared in this evolutionary ancient cell as a nutrient receptor.


Subject(s)
Paramecium/metabolism , Receptors, Adrenergic, beta-2/metabolism , Animals , Binding Sites , Blotting, Western , Cell Fractionation , Cell Membrane/chemistry , Cell Membrane/immunology , Electrophoresis, Polyacrylamide Gel , Humans , Isoproterenol/pharmacology , Microscopy, Confocal , Microscopy, Immunoelectron , Molecular Weight , Paramecium/immunology , Paramecium/ultrastructure , Receptors, Adrenergic, beta-2/immunology , Receptors, Adrenergic, beta-2/isolation & purification , Transport Vesicles/chemistry , Transport Vesicles/immunology , Vertebrates/immunology , Vertebrates/metabolism
8.
J Eukaryot Microbiol ; 48(2): 234-45, 2001.
Article in English | MEDLINE | ID: mdl-12095113

ABSTRACT

In Paramecium, several kinds of the oral networks of fine filaments are defined at the ultrastructural level. Using the sodium chloride-treated oral apparatus of Paramecium as an antigen to produce monoclonal antibodies, we have begun to identify the proteins constituting these networks. Immunoblotting showed that all positive antibodies were directed against three bands (70-, 75-and 83-kD), which corresponded to quantitatively minor components of the antigen; there was no antibody specific for the quantitatively major components (58- and 62-kD). Immunolocalization with four of these antibodies directed against one or several of these three bands showed that these proteins are components of the fine filaments supporting the oral area; a decoration of the basal bodies and the outer lattice was also observed on the cortex. Immunofluorescence on interphase cells suggested that the three proteins colocalized on the left side of the oral apparatus, whereas only the 70-kD band was detected on the right side. During division, the antigens of the antibodies were detected at different stages after oral basal body assembly. The antibodies cross-reacted with the tetrins, which are oral filament-forming proteins in Tetrahymena, demonstrating that tetrin-related proteins are quantitatively minor components of the oral and the somatic cytoskeleton of Paramecium.


Subject(s)
Paramecium/chemistry , Paramecium/ultrastructure , Protozoan Proteins/analysis , Animals , Antibodies, Monoclonal/immunology , Antibodies, Protozoan/immunology , Antigens, Protozoan/analysis , Cytoskeletal Proteins/immunology , Epitopes , Immunoblotting , Immunohistochemistry , Interphase , Microscopy, Fluorescence , Microscopy, Immunoelectron , Morphogenesis , Paramecium/growth & development , Paramecium/immunology , Protozoan Proteins/immunology
9.
J Membr Biol ; 172(1): 77-88, 1999 Nov 01.
Article in English | MEDLINE | ID: mdl-10552016

ABSTRACT

We combined widely different biochemical methods to analyze proteins of the cell surface of P. tetraurelia since so far one can isolate only a subfraction of cell membrane vesicles enriched in the GPI-anchored surface antigens ("immoblization" or "i-AGs"). We also found that i-AGs may undergo partial degradation by endogenous proteases. Genuine intrinsic membrane proteins were recognized particularly with lipophilic 5-[(125)I]-iodonaphthalene-1-azide (INA) labeling which reportedly "sees" integral proteins and cytoplasmic cell membrane-associated proteins. With INA (+DTT), bands of

Subject(s)
Paramecium/chemistry , Protozoan Proteins/isolation & purification , Animals , Antigens, Protozoan/chemistry , Antigens, Protozoan/isolation & purification , Antigens, Surface/chemistry , Antigens, Surface/isolation & purification , Azides , Cell Membrane/chemistry , Cholic Acids , Cross-Linking Reagents , Iodine Radioisotopes , Membrane Proteins/chemistry , Membrane Proteins/immunology , Membrane Proteins/isolation & purification , Molecular Weight , Paramecium/immunology , Protozoan Proteins/chemistry , Protozoan Proteins/immunology , Solubility
10.
J Eukaryot Microbiol ; 45(2): 202-9, 1998.
Article in English | MEDLINE | ID: mdl-9561774

ABSTRACT

The pellicle of Paramecium has three two-dimensionally arrayed systems that occupy separate but closely paralleling planes. All three systems are now distinguishable by their differing immunological properties. This study focused on the two deeper systems. The infraciliary lattice lies innermost and labels with centrin-specific antibodies. The middle system, the striated bands, is specifically labeled with a monoclonal antibody that we have raised to a 110 kDa cortical antigen in P. multimicronucleatum. This antibody labels a similar geometric cortical pattern in at least two species, P. multimicronucleatum and P. tetraurelia. Centrin-specific structures appear to be net-like in the above two species but show a more interrupted pattern in P. caudatum. The cytostomal cord is an essentially unbranched extension of the net-like infraciliary lattice and, like it, is centrin-specific. The cord has a unique association with the alveolar sacs which suggest these calcium-storing compartments contribute to the calcium fluxes required for contraction of the cord. A structural rather than a contractile function is favored for the striated bands, based solely on their morphology.


Subject(s)
Antibodies, Monoclonal/immunology , Antigens, Protozoan/immunology , Calcium-Binding Proteins/immunology , Chromosomal Proteins, Non-Histone , Cytoskeleton/immunology , Paramecium/immunology , Protozoan Proteins/immunology , Animals , Antibodies, Protozoan/immunology , Blotting, Western , Cytoskeletal Proteins/immunology , Cytoskeleton/ultrastructure , Electrophoresis, Polyacrylamide Gel , Fluorescent Antibody Technique, Indirect , Freeze Etching , Immunohistochemistry , Mice , Mice, Inbred BALB C , Microscopy, Electron , Paramecium/ultrastructure
11.
J Eukaryot Microbiol ; 43(4): 303-13, 1996.
Article in English | MEDLINE | ID: mdl-8768434

ABSTRACT

When paramecium primaurelia expresses the D serotype, a major high molecular weight mRNA species is detected in the cytoplasm. Using the cDNA derived from this mRNA as a probe, three very similar genes, D alpha, D beta and D gamma, were cloned. Of these three genes, we show that only the D alpha mRNA is present in the cytoplasm of cells expressing the D serotype and corresponds to the major mRNA species. The nucleotide sequence of the entire coding region of the D alpha gene, as well as the upstream and downstream sequences, has been determined. The 7632-nucleotide open reading frame encodes a putative protein that displays the characteristic cysteine residue periodicity of Paramecium surface antigens but does not contain central tandemly repeated sequences. Partial sequences of the two nonexpressed genes D beta and D gamma indicate a high percentage of identity (90%-95%) with the D alpha gene, suggesting that D beta and D gamma genes are either very similar surface protein genes whose transcription is repressed trough mutual exclusion, or perhaps are pseudogenes. A region of variable DNA rearrangement was identified 1 kb upstream of the D gamma gene. This macronuclear region arises from the same micronuclear locus by alternative excision of internal eliminated sequences during macronuclear development.


Subject(s)
Antigens, Protozoan/genetics , Antigens, Surface/genetics , Paramecium/immunology , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , DNA Probes , Gene Expression , Gene Rearrangement , Molecular Sequence Data , Oligodeoxyribonucleotides
12.
J Cell Sci ; 108 ( Pt 3): 1263-74, 1995 Mar.
Article in English | MEDLINE | ID: mdl-7622609

ABSTRACT

The extent to which a donor membrane will be retrieved, or if it is retrieved at all after it fuses with an acceptor membrane, is usually difficult to determine. We have studied the dynamics of membrane retrieval in the phagosome system of Paramecium multimicronucleatum using six monoclonal antibody markers. Our previous freeze-fracture and transmission electron microscopic studies have indicated that extensive changes take place in the membrane of the young phagosome as it progresses through its cycle. Using immunofluorescence and immunoelectron microscopy to determine the times of entry and exit of these individual antigens into the digestive vacuole system, we showed that two hydrophilic antigens, one located on the cytosolic and one on the lumenal side of the discoidal membrane (phagosome membrane precursor), were completely retrieved from the phagosome by tubulation within the first three minutes. At the same time that this membrane was retrieved, membrane from a second population of vesicles, the acidosomes, fused with the phagosome to produce the phagoacidosome. On the basis of immunogold localization on cryosections of a total of six antigens, the two specific for phagosome/discoidal vesicle membrane as well as four specific for the acidosome/phagoacidosome membrane, this replacement is total. We also showed that in the presence of the actin-active drug cytochalasin B, this replacement was essentially prevented. However, when vacuole acidification was neutralized by ammonium chloride, this replacement process continued unaffected after a lag. Consequently, acidification, per se, is not required to trigger the replacement of the phagosome membrane. We conclude, on the basis of these studies as well as our previous freeze-fracture studies that during phagoacidosome formation most of the acceptor membrane is retrieved and is replaced by the donor membrane. This shows that at least one cell type possesses the mechanisms needed to substantially replace the membrane of a phagosomal compartment when radical and rapid changes are needed to modulate the digestive and absorptive processes.


Subject(s)
Membrane Fusion/physiology , Paramecium/physiology , Phagosomes/physiology , Ammonium Chloride/pharmacology , Animals , Antibodies, Monoclonal , Antigens, Protozoan/chemistry , Antigens, Protozoan/metabolism , Cytochalasin B/pharmacology , Frozen Sections , Membrane Fusion/drug effects , Microscopy, Fluorescence , Microscopy, Immunoelectron , Paramecium/immunology , Paramecium/ultrastructure , Phagosomes/drug effects , Phagosomes/ultrastructure
13.
J Eukaryot Microbiol ; 41(5): 503-10, 1994.
Article in English | MEDLINE | ID: mdl-7804254

ABSTRACT

A monoclonal antibody (mAb) IR-2-1 was raised against a 67-kDa protein purified from the macronucleus-specific bacterial symbiont Holospora obtusa of Paramecium caudatum. The mAb was found to react with two bands (31 and 67-kDa) on gels of H. obtusa. Indirect immunofluorescence microscopy showed that these antigens were distributed inside the cells. However, unexpectedly, this mAb also cross reacted with the radial arms of the contractile vacuole in P. caudatum, P. tetraurelia, P. multimicronucleatum, P. jenningsi and P. bursaria as well as with their cytoplasm. Immunoelectron microscopy showed that the antigens were located on the decorated spongiome of the radial arms. In immunoblots, mAb IR-2-1 reacted with a band of 67 kDa in all Paramecium species examined. However, no band appeared in the immunoblot of isolated macronuclei of H. obtusa-free P. caudatum and no label was seen in the nuclear matrix of the macronucleus of air-dried P. caudatum. These results suggest that the 67-kDa antigen found in H. obtusa was not imported from the host macronucleus and the same antigen in the host contractile vacuoles and cytoplasm were not derived from the symbiont. These results also showed that an epitope on the decorated spongiome of the Paramecium species is shared by its bacterial symbiont. In contrast to the decorated tubule-specific mAb, DS-1, the antigens for IR-2-1 appeared to be loosely membrane bound as they were lost in paraformaldehyde fixed and acetone permeabilized Paramecium.


Subject(s)
Antibodies, Monoclonal , Gram-Negative Bacteria/immunology , Paramecium/immunology , Paramecium/microbiology , Animals , Cell Nucleus/microbiology , Cross Reactions , Fluorescent Antibody Technique , Microscopy, Immunoelectron , Paramecium/ultrastructure , Protozoan Proteins/immunology , Symbiosis , Vacuoles/immunology
14.
Biol Cell ; 81(2): 95-119, 1994.
Article in English | MEDLINE | ID: mdl-7531532

ABSTRACT

Ciliates are very good models for studying post-translationally generated tubulin heterogeneity because they exhibit highly differentiated microtubular networks in combination with reduced genetic diversity. We have approached the analysis of tubulin heterogeneity in Paramecium through extensive isolation and characterization of monoclonal antibodies using various antigens and several immunization protocols. Eight monoclonal antibodies and 10 hybridoma supernatants were characterized by: i) immunoblotting on ciliate and pig brain tubulins as well as on peptide maps of Paramecium axonemal tubulin; ii) immunoblotting on ciliate tubulin fusion peptides generated in E coli, a procedure which allows in principle to discriminate antibodies that are directed against tubulin sequence (reactive on fusion peptides) from those directed against a post-translational epitope (non-reactive); and iii) immunofluorescence on Paramecium, 3T3 and PtK2 cells. Twelve antibodies labeled all microtubules in Paramecium cells and were found to be directed against tubulin primary sequences (nine of them being located in the alpha N-terminal domain, one in the beta C-terminal one, and two in alpha and beta central stretches). The remaining ones decorated only a specific subset of microtubules within the cell and were presumably directed against post-translational modifications. Among these, three antibodies are directed against an N-terminal acetylated epitope of alpha-tubulin whereas the epitopes of three other ones (TAP 952 degrees, AXO 58 and AXO 49 degrees) apparently correspond to still unidentified post-translational modifications, located in the C-terminal domain of both alpha- and beta-tubulins. The AXO 49 degrees specificity is similar to that of a previously described polyclonal serum raised against Paramecium axonemal tubulin [2]. The results are discussed in terms of identification and accessibility of the epitopes and immunogenicity of ciliate tubulin with reference to mammalian and ciliate tubulin sequences.


Subject(s)
Antibodies, Monoclonal/immunology , Paramecium/immunology , Tubulin/immunology , Amino Acid Sequence , Animals , Antibodies, Monoclonal/isolation & purification , Antibody Specificity , Binding Sites, Antibody , Brain/metabolism , Cell Line , Cilia/immunology , Epitopes/immunology , Immunoblotting , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Protein Processing, Post-Translational , Swine
15.
Cell Motil Cytoskeleton ; 29(3): 215-24, 1994.
Article in English | MEDLINE | ID: mdl-7895285

ABSTRACT

In an earlier study we reported the isolation of a cytoplasmic dynein from the cytosol of Paramecium multimicronucleatum. In this study we report the isolation and characterization of two cytosolic axonemal dyneins (22S and 12S) as well as a 19S cytoplasmic dynein from the cytosol of whole or deciliated cells using preformed bovine brain microtubules. These three dynein species were characterized according to mass, morphology, vanadate photocleavage patterns, CTPase/ATPase ratios, Km and Vmax values, temperature optima and reactivity with a mAb. For comparison, 22S and 12S axonemal dyneins (ADs) were also isolated and purified from the demembranated axonemes. The 22S and 12S soluble dyneins appear to be related to ciliary ADs in that the 22S soluble dynein is three-headed while the 12S is a one-headed dynein, as determined by negative staining. Ciliary ADs and their corresponding 22S and 12S soluble dyneins isolated from the cytosol also have similar Km and Vmax values as well as vanadate photocleavage patterns and temperature optima. A mAb raised against the soluble 22S dynein reacted with the 22S ciliary dyneins but not the 12S axonemal or the 19S cytoplasmic dynein. All isolated dyneins supported similar microtubule gliding rates but had different ionic requirements for the translocation buffer. These results suggest that: (i) the two soluble 22S and 12S dyneins are precursor molecules of the ciliary dyneins, (ii) the subunits of the outer arm dynein are already assembled in the cytosol as a three-headed bouquet, and (iii) the 22S and 12S soluble dyneins are functional prior to being transported and attached to the axonemes of the cilia.


Subject(s)
Cilia/metabolism , Dyneins/metabolism , Paramecium/metabolism , Protozoan Proteins/metabolism , Animals , Antibodies, Monoclonal/immunology , Antibodies, Protozoan/immunology , Biological Transport , Chemical Phenomena , Chemistry, Physical , Cytosol/metabolism , Dyneins/immunology , Molecular Weight , Negative Staining , Paramecium/immunology , Paramecium/ultrastructure , Protein Folding , Protozoan Proteins/immunology , Reproducibility of Results
16.
Cell Motil Cytoskeleton ; 27(4): 337-49, 1994.
Article in English | MEDLINE | ID: mdl-7520839

ABSTRACT

Microtubular networks are extensively developed in many ciliate species. In several of them, we investigate the occurrence of the post-translational glutamylation of tubulin [Eddé et al., 1990: Science 247:82-85; Eddé et al., 1991: J. Cell. Biochem. 46:134-142] using as a probe for such modified tubulin, the monoclonal antibody GT335 [Wolff et al., 1992: Eur. J. Cell Biol. 59:425-432]. Results obtained in Paramecium strongly suggest that both axonemal and cytoplasmic tubulin are glutamylated. As in the vertebrate brain tubulin so far tested, the GT335 epitope is located at the carboxy-terminal fragment of cytoplasmic tubulin removed by subtilisin treatment. Immunoblotting and immunofluorescence experiments reveal that, unlike tubulin acetylation, glutamylation is not restricted to cold-resistant microtubules. In addition, immunofluorescence studies performed on dividing cells show that glutamylation takes place soon after the polymerization of microtubules. Finally, glutamylated tubulin is also detected in the ciliate species Euplotes, Tetrahymena, and Paraurostyla. Together with results obtained on flagellate species, this suggests that tubulin glutamylation came out early in the course of eukaryotic evolution and has been widely exploited in various cellular strategies.


Subject(s)
Cilia/chemistry , Eukaryota/chemistry , Glutamates , Tubulin/chemistry , Animals , Antibodies, Monoclonal , Cytoplasm/chemistry , Epitopes , Eukaryota/immunology , Eukaryota/ultrastructure , Glutamic Acid , Microtubules/chemistry , Molecular Probes , Paramecium/immunology
17.
J Cell Sci ; 106 ( Pt 2): 693-702, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8282774

ABSTRACT

In a previous study, monoclonal antibody DS-1 was found to specifically label the decorated spongiome along the radial arms of the contractile vacuole complexes in Paramecium multimicronucleatum. Fluorescein isothiocyanate-conjugated DS-1, when injected into cells, labels the radial arms initially, but with increasing postinjection time both the intensity of fluorescence and the number of fluorescently labeled radial arms were reduced. When these cells were fixed after 45 minutes and probed indirectly using a second fluorochrome, little new label was seen on the already fluorescein-labeled radial arms. Thin sections showed that the amount of decorated tubules along some collecting canals decreased from the proximal to the distal end and vesicles, which were never seen in control cells, appeared next to the decorated spongiome. These results suggested that the decorated spongiome was undergoing disassembly and sequestration into one region of the cell. The injected DS-1 also reduced the expulsion frequency of the contractile vacuoles in a dose-, time- and site-dependent manner. The contractile vacuole complexes near the injection site were affected more than those farther from the site, but the sizes of both contractile vacuoles were only transiently affected so that fluid output per cell was reduced by approximately 60%. Beyond 45 minutes postinjection, both the expulsion frequency and total fluid output began to recover as the DS-1 was sequestered into one part of the cell. This region persisted in cells up to 18 hours but disappeared by 24 hours, which coincided with the full recovery of the expulsion frequency and of decorated spongiome along the radial arms. The contractile vacuole, the collecting canals and the smooth spongiome were morphologically unaffected. These results indicate that when the decorated spongiome is dissociated from the contractile vacuole complex, the complex's function is strongly inhibited, showing the decorated spongiome to be the site of fluid segregation.


Subject(s)
Paramecium/physiology , Water-Electrolyte Balance/physiology , Animals , Antibodies, Monoclonal , Fluorescein-5-isothiocyanate , Intracellular Fluid/physiology , Microscopy, Electron , Paramecium/immunology , Paramecium/ultrastructure , Vacuoles/immunology , Vacuoles/physiology , Vacuoles/ultrastructure
18.
Biol Cell ; 75(3): 217-23, 1992.
Article in English | MEDLINE | ID: mdl-1335331

ABSTRACT

Biosynthetic labelling experiments performed on P primaurelia strain 156, expressing the temperature-specific G surface antigen, 156G SAg, demonstrated that the purified 156G SAg contained the components characteristic of a GPI-anchor. [3H]ethanolamine, [3H]myo-inositol, [32P]phosphoric acid and [3H]myristic acid could all be incorporated into the surface antigen. Myristic acid labelling was lost after treatment in vitro with Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PI-PLC). After complete digestion by pronase, a fragment containing the intact GPI-anchor of 156G surface antigen was isolated. This fragment was shown to be hydrophobic and glycosylated and to possess an epitope found specifically in the GPI component of GPI-anchored proteins. The role of the GPI-tail in anchoring the 156G surface antigen into the membrane was assessed by determining that purified 156G molecules with the GPI-anchor could be incorporated into lipid vesicles and red cell ghosts whereas the 156G molecules lacking the GPI-anchor, as result of treatment with B thuringiensis PI-PLC, could not. It has also been shown that the membrane-bound form and the soluble form, obtained after cleavage of the 156G SAg lipid moiety either by an endogenous PI-PLC or by a bacterial PI-PLC, displayed identical circular dichroic spectra.


Subject(s)
Antigens, Protozoan/chemistry , Antigens, Surface/chemistry , Membrane Glycoproteins/chemistry , Paramecium/immunology , Animals , Circular Dichroism , Glycosylphosphatidylinositols/analysis , Liposomes , Phosphatidylinositol Diacylglycerol-Lyase , Phosphoinositide Phospholipase C , Phosphoric Diester Hydrolases/metabolism , Protein Conformation , Solubility , Temperature
19.
Dev Genet ; 13(1): 53-7, 1992.
Article in English | MEDLINE | ID: mdl-1395142

ABSTRACT

We obtained a monoclonal antibody (MA-1) specific for macronuclei of the ciliate Paramecium caudatum and P. dubosqui. Immunoblotting showed that the antigen was a polypeptide of 50 kilodalton (kDa). During the process of nuclear differentiation in P. caudatum, the MA-1 antigens appeared in the macronuclear anlagen immediately after four out of eight post zygotic nuclei differentiated morphologically into the macronuclear anlagen. Afterwards, the antigens could be detected in the macronucleus through the cell cycle, and disappeared when the macronucleus began to degenerate in exconjugant cells. These results suggest that the antigens may play a role in the differentiation and function of the macronucleus.


Subject(s)
Antigens, Protozoan/metabolism , Cell Nucleus/immunology , Paramecium/growth & development , Animals , Antibodies, Monoclonal , Cross Reactions , Fluorescent Antibody Technique , Immunoblotting , Kinetics , Paramecium/immunology
20.
Mol Cell Biol ; 11(2): 1133-7, 1991 Feb.
Article in English | MEDLINE | ID: mdl-1990269

ABSTRACT

A previously isolated mutant cell line called d48 contains a complete copy of the A surface antigen gene in the micronuclear genome, but the gene is not incorporated into the macronucleus. Previous experiments have shown that a cytoplasmic factor made in the wild-type macronucleus can rescue the mutant. Recently, S. Koizumi and S. Kobayashi (Mol. Cell. Biol. 9:4398-4401, 1989) observed that injection of a plasmid containing the A gene into the d48 macronucleus rescued the cell line after autogamy. It is shown here that an 8.8-kb EcoRI fragment containing only a portion of the A gene coding region is sufficient for the rescue of d48. The inability of other A gene fragments to rescue the mutant shows that this effect is dependent upon specific Paramecium DNA sequences. Rescue results in restoration of the wild-type DNA restriction pattern in the macronucleus. These results are consistent with a model in which the macronuclear A locus normally makes an additional gene product that is required for correct processing of the micronuclear copy of the A gene.


Subject(s)
Antigens, Protozoan/genetics , Antigens, Surface/genetics , Cell Nucleus/physiology , DNA/genetics , Paramecium/physiology , Animals , Blotting, Southern , Cloning, Molecular , DNA/isolation & purification , Molecular Weight , Mutation , Nucleic Acid Hybridization , Paramecium/genetics , Paramecium/immunology , Plasmids , Restriction Mapping
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