ABSTRACT
Early endometriosis is associated with infertility, and oxidative stress may play a role in the pathogenesis of disease-related infertility. This prospective case-control study aimed to compare the presence of oxidative stress markers in the follicular microenvironment and systemic circulation of infertile women with minimal/mild endometriosis (EI/II) versus individuals undergoing controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI). Seventy-one blood samples (27 from infertile women with EI/II and 44 controls with tubal and/or male infertility factor) and 51 follicular fluid samples (19 EI/II and 32 controls) were obtained on the day of oocyte retrieval. Total hydroperoxides (FOX1 ), reduced glutathione, vitamin E, Superoxide dismutase, total antioxidant capacity, malondialdehyde, advanced oxidation protein products, and 8-hydroxy-2'-deoxyguanosine (8OHdG) concentrations were measured in both fluids. Women with EI/II showed higher FOX1 (8.48 ± 1.72 vs. 7.69 ± 1.71 µmol/g protein) and lower total antioxidant capacity (0.38 ± 0.18 vs. 0.46 ± 0.15 mEq Trolox/L) concentrations in serum, and higher 8OHdG concentrations (24.21 ± 8.56 vs. 17.22 ± 5.6 ng/ml) in follicular fluid compared with controls. These data implicate both systemic and follicular oxidative stress may in infertile women with EI/II undergoing controlled ovarian stimulation for ICSI. Furthermore, the elevated 8OHdG concentrations in follicular fluid of women with EI/II may be related to compromised oocyte quality.
Subject(s)
DNA Damage/physiology , Endometriosis , Infertility, Female/etiology , Oocytes/metabolism , Oxidative Stress/physiology , Pelvic Floor Disorders , Adult , Antioxidants/metabolism , Case-Control Studies , Cohort Studies , Endometriosis/complications , Endometriosis/genetics , Endometriosis/metabolism , Endometriosis/pathology , Female , Follicular Fluid/chemistry , Follicular Fluid/metabolism , Humans , Infertility, Female/genetics , Infertility, Female/metabolism , Oocytes/chemistry , Oxidative Stress/genetics , Pelvic Floor Disorders/complications , Pelvic Floor Disorders/genetics , Pelvic Floor Disorders/metabolism , Pelvic Floor Disorders/pathology , Severity of Illness IndexABSTRACT
BACKGROUND: Pelvic floor dysfunction (PFD) is a condition affecting many women worldwide, with symptoms including stress urinary incontinence (SUI) and pelvic organ prolapse (POP). We have previously demonstrated stable elastin-expressing bone marrow-derived mesenchymal stem cells (BMSCs) attenuated PFD in rats, and aim to further study the effect of microRNA-29a-3p regulation on elastin expression and efficacy of BMSC transplantation therapy. METHODS: We inhibited endogenous microRNA-29a-3p in BMSCs and investigated its effect on elastin expression by RT-PCR and Western blot. MicroRNA-29-inhibited BMSCs were then transplanted into PFD rats, accompanied by sustained release of bFGF using formulated bFGF in poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NP), followed by evaluation of urodynamic tests. RESULTS: MicroRNA-29a-3p inhibition resulted in upregulated expression and secretion of elastin in in vitro culture of BMSCs. After co-injection with PLGA-loaded bFGF NP into the PFD rats in vivo, microRNA-29a-3p-inhibited BMSCs significantly improved the urodynamic test results. CONCLUSIONS: Our multidisciplinary study, combining microRNA biology, genetically engineered BMSCs, and nanoparticle technology, provides an excellent stem cell-based therapy for repairing connective tissues and treating PFD.
Subject(s)
Bone Marrow Cells/metabolism , Bone Marrow/metabolism , Elastin/metabolism , Mesenchymal Stem Cells/metabolism , MicroRNAs/metabolism , Pelvic Floor Disorders/metabolism , Pelvic Floor Disorders/therapy , Animals , Bone Marrow/drug effects , Bone Marrow Cells/drug effects , Cells, Cultured , Disease Models, Animal , Fibroblast Growth Factors/administration & dosage , Humans , Lactic Acid/administration & dosage , Mesenchymal Stem Cell Transplantation/methods , Mesenchymal Stem Cells/drug effects , Nanoparticles/administration & dosage , Pelvic Floor/physiology , Polyglycolic Acid/administration & dosage , Polylactic Acid-Polyglycolic Acid Copolymer , RatsABSTRACT
INTRODUCTION AND HYPOTHESIS: This study aims to evaluate the effects of simulated birth trauma and vaginal and Cesarean delivery on glycosaminoglycans (GAGs) in the vagina of female rats. METHODS: One hundred ten rats were divided into six groups: A (control), B (vaginal trauma), C (Cesarean delivery), D (Cesarean delivery followed by vaginal trauma), E (vaginal delivery), and F (20th day of gestation). In each group, half of the animals were killed 4 days after the procedure (time 1) and 12 weeks later (time 2). GAGs were extracted, isolated, and identified by using agarose gel electrophoresis and quantified by densitometry. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney tests. RESULTS: We observed a significant decrease in total GAGs and dermatan sulfate (DS) at time 1. Evaluation at time 2 showed a significant increase in total GAGs, DS, and heparan sulfate. CONCLUSIONS: Levels of sulfated GAGs in the rat vagina are affected by delivery and simulated birth trauma.
Subject(s)
Glycosaminoglycans/metabolism , Parturition/metabolism , Pelvic Floor Disorders/metabolism , Postpartum Period/metabolism , Pregnancy, Animal/metabolism , Vagina/metabolism , Animals , Cesarean Section , Dermatan Sulfate/metabolism , Female , Heparitin Sulfate/metabolism , Models, Animal , Pregnancy , Rats , Rats, WistarABSTRACT
El riñón pélvico se define a aquel con una ubicación diferente a la habitual y localizado a nivel de la pelvis fetal. Se describe un caso de riñón pélvico diagnosticado intraútero y se realiza una revisión sobre el manejo y significado de este hallazgo ultrasonográfico (AU)
The pelvic kidney is defined to that with a location different from the habitual one and located at level of pelvis fetal. A case of pelvic kidney diagnosed intrauterus is described and it is made an overhaul on the handling and meaning of this ultrasonographic finding (AU)
