ABSTRACT
Diseases resulting from Helicobacter pylori infection appear to be dependent on a host of genetic traits and virulence factors possessed by this microorganism. This paper aimed to investigate the association between the ABO histo-blood groups and H. pylori cagA infections. Genomic DNA samples (n = 110) of gastric biopsies obtained from patients with endoscopic diagnosis of peptic ulcers (n = 25) and chronic active gastritis (n = 85) were analyzed by PCR using specific primers for the cagA gene. Of the samples, 66.4 percent (n = 73) tested positive and 33.6 percent (n = 37) negative for the gene. The cagA strain was predominant in peptic ulcers (n = 21; 84.0 percent) compared with chronic active gastritis (n = 52; 61.2 percent) (p = 0.05; OR 3.332; 95 percent CI: 1.050-10.576). Additionally, the cagA strain was prevalent in the type O blood (48/63; 76.2 percent) compared with other ABO phenotypes (25/47; 53.2 percent) (p = 0.01; OR 2.816; 95 percent CI: 1.246-6.364). These results suggest that H. pylori cagA infection is associated with the O blood group in Brazilian patients suffering from chronic active gastritis and peptic ulcers.(AU)
Subject(s)
Humans , Male , Female , Helicobacter pylori , Helicobacter Infections/genetics , Gastritis/blood , Peptic Ulcer/blood , ABO Blood-Group SystemABSTRACT
Diseases resulting from Helicobacter pylori infection appear to be dependent on a host of genetic traits and virulence factors possessed by this microorganism. This paper aimed to investigate the association between the ABO histo-blood groups and H. pylori cagA infections. Genomic DNA samples (n = 110) of gastric biopsies obtained from patients with endoscopic diagnosis of peptic ulcers (n = 25) and chronic active gastritis (n = 85) were analyzed by PCR using specific primers for the cagA gene. Of the samples, 66.4 percent (n = 73) tested positive and 33.6 percent (n = 37) negative for the gene. The cagA strain was predominant in peptic ulcers (n = 21; 84.0 percent) compared with chronic active gastritis (n = 52; 61.2 percent) (p = 0.05; OR 3.332; 95 percent CI: 1.050-10.576). Additionally, the cagA strain was prevalent in the type O blood (48/63; 76.2 percent) compared with other ABO phenotypes (25/47; 53.2 percent) (p = 0.01; OR 2.816; 95 percent CI: 1.246-6.364). These results suggest that H. pylori cagA infection is associated with the O blood group in Brazilian patients suffering from chronic active gastritis and peptic ulcers.
Subject(s)
Humans , Male , Female , ABO Blood-Group System , Gastritis/blood , Helicobacter pylori , Helicobacter Infections/genetics , Peptic Ulcer/bloodABSTRACT
AIM: Triple therapy seems more effective in curing Helicobacter pylori infection in patients with peptic ulcer than in those with non-ulcer dyspepsia. It has been suggested that this difference depends on the expression of CagA protein that is more frequent in the former. The objective of this study was to investigate a potential association between serum CagA positivity, severity of gastric mucosal inflammation and eradication success among peptic ulcer and non-ulcer dyspepsia patients. MATERIAL AND METHOD: Patients undergoing upper gastrointestinal endoscopy for investigation of dyspepsia at the Department of Gastroenterology, Hospital Vera Cruz, between March, 2000 and March 2001 were screened. H. pylori positive patients, as diagnosed by rapid urease test and histology were included. Severity of gastric mucosal inflammation was determined and serum CagA positivity was assessed using a commercially available ELISA assay prior to H. pylori 7-day eradication therapy with lansoprazole, clarithromycin and amoxicillin (30 mg, 500 mg and 1 g b.i.d., respectively). Eradication success was determined 8-24 weeks following completion of therapy. RESULTS: Seventy-four patients were included in the study (mean age 40.8, range 18-67, female = 28). CagA positivity was observed in 48% of patients. Gastroduodenal peptic ulceration was found in 54% of patients. Serum CagA positivity was significantly higher among peptic ulcer patients (62.5%), while CagA negativity was significantly higher among non-ulcer dyspepsia patients (67.7%). Lymphocyte and eosinophil infiltration was significantly higher among CagA + patients, despite being comparable when distributed among peptic ulcer and non-ulcer dyspepsia patients. Eradication was successful in 93.2% of patients, regardless of CagA status on a per protocol analysis. Based on a per protocol analysis, eradication success was comparable among peptic ulcer and non-ulcer dyspepsia patients, regardless of CagA status. CONCLUSION: Our results support the concept that CagA positivity is associated to peptic ulcer disease and to a higher severity of lymphocyte and eosinophil infiltration. Efficacy of treatment eradication of H. pylori may not be affected by serum CagA status.
Subject(s)
Amoxicillin/therapeutic use , Anti-Bacterial Agents/therapeutic use , Anti-Ulcer Agents/therapeutic use , Antigens, Bacterial/blood , Bacterial Proteins/blood , Clarithromycin/therapeutic use , Dyspepsia/etiology , Gastritis/drug therapy , Helicobacter Infections/drug therapy , Helicobacter pylori/isolation & purification , Omeprazole/analogs & derivatives , Peptic Ulcer/etiology , 2-Pyridinylmethylsulfinylbenzimidazoles , Adolescent , Adult , Aged , Amoxicillin/administration & dosage , Anti-Bacterial Agents/administration & dosage , Anti-Ulcer Agents/administration & dosage , Antigens, Bacterial/genetics , Bacterial Proteins/genetics , Biomarkers , Clarithromycin/administration & dosage , Drug Therapy, Combination , Dyspepsia/microbiology , Enzyme-Linked Immunosorbent Assay , Eosinophilia/blood , Eosinophilia/etiology , Eosinophilia/pathology , Eosinophils/pathology , Female , Gastric Mucosa/microbiology , Gastric Mucosa/pathology , Gastritis/blood , Gastritis/complications , Gastritis/microbiology , Gastritis/pathology , Helicobacter Infections/complications , Helicobacter Infections/pathology , Helicobacter pylori/genetics , Humans , Lansoprazole , Lymphocytes/pathology , Male , Middle Aged , Omeprazole/administration & dosage , Omeprazole/therapeutic use , Peptic Ulcer/blood , Peptic Ulcer/microbiology , Peptic Ulcer/pathologyABSTRACT
OBJETIVO: determinar la distribución de los grupos sanguineos ABO en los pacientes con úlcera péptica. METODOS: trabajo descriptivo y retrospectivo. Se revisaron historias clínicas de pacientes con diagnóstico endoscópico de úlcera péptica y tipificación del grupo sanguineo ABO entre junio de 1994 y noviembre de 1996. Se tomó como control los pacientes y donantes del banco de sangre atendidos entre julio y agosto de 1996. El método estadístico utilizado fue el chi-cuadrado. RESULTADOS: se estudiaron 100 pacientes. En el control, 73 por ciento tuvieron el grupo sanguíneo O, en los pacientes con úlcera péptica 78 por ciento. De los ulcerosos duodenales, 85 por ciento presentaron grupo sanguineo O y de éstos, los menores de 40 años, 94 por ciento tenía grupo O. CONCLUSIONES: 1. la distribución de grupos sanguíneos en los pacientes con úlcera péptica es similar al control; 2. la distribución de grupos ABO en pacientes con úlcera duodenal muestra una diferencia estadísticamente significativa respecto al control con predominio del grupo O, particularmente en los pacientes menores de 40 años.
Subject(s)
ABO Blood-Group System/analysis , ABO Blood-Group System/administration & dosage , Peptic Ulcer/bloodSubject(s)
Gastrins/blood , Omeprazole/therapeutic use , Pepsinogens/blood , Peptic Ulcer/drug therapy , Administration, Oral , Biopsy , Child, Preschool , Endoscopy, Gastrointestinal , Female , Humans , Infusions, Intravenous , Omeprazole/administration & dosage , Peptic Ulcer/blood , Peptic Ulcer/pathology , Pyloric Antrum/pathology , Zollinger-Ellison Syndrome/blood , Zollinger-Ellison Syndrome/drug therapy , Zollinger-Ellison Syndrome/pathologyABSTRACT
Se realiza el estudio de 763 pacientes con enfermedad ulcerosa péptica gastro-duodenal, en el Hospital Regional "Honorio Delgado" de Arequipa (2300 metros sobre el nivel del mar), comparando las características de los pacientes de la Costa y la Altura. Se concluye que los pacientes de Altura la enfermedad ulcerosa se presenta en edades más tempranas (tercera década); predomina la presentación clínica atípica; tiene una mayor incidencia de complicaciones (59 por ciento), básicamente hemorragia (53 por ciento); la localización gástrica es más frecuente (UD/UG=2.5/1; versus 5/1 en pacientes procedentes de la costa) y la mortalidad en ellos es más elevada (2.0 por ciento)
Subject(s)
Humans , Duodenogastric Reflux/nursing , Peptic Ulcer/diagnosis , Peptic Ulcer/bloodABSTRACT
Se analizó la relación de los grupos sanguíneos con la patología gastroduodenal en 276 enfermos operados por carcinoma gástrico y úlceras gástricas y duodenales con un grupo control de 200 colecistectomizados. Se llegó a la conclusión de una mayor incidencia de carcinoma gástrico en el grupo A y de úlcera duodenal en el grupo O2 no obteniéndose resultados concluyentes respecto a la úlcera gástrica (AU)
Subject(s)
Humans , Male , Female , Blood Group Antigens , Stomach Neoplasms/blood , Stomach Ulcer/blood , Peptic Ulcer/blood , ArgentinaABSTRACT
Se analizó la relación de los grupos sanguíneos con la patología gastroduodenal en 276 enfermos operados por carcinoma gástrico y úlceras gástricas y duodenales con un grupo control de 200 colecistectomizados. Se llegó a la conclusión de una mayor incidencia de carcinoma gástrico en el grupo A y de úlcera duodenal en el grupo O2 no obteniéndose resultados concluyentes respecto a la úlcera gástrica