Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 16 de 16
Filter
1.
J Gastroenterol Hepatol ; 22(5): 749-56, 2007 May.
Article in English | MEDLINE | ID: mdl-17444866

ABSTRACT

BACKGROUND: Candida sp are frequently isolated from the ascitic fluid of patients with perforated ulcers. The present study was performed to examine whether Candida infection may be involved in the process of ulcer perforation. METHODS: Male Wistar rats were divided into a saline group (n = 15) and a Candida group (n = 17). Cysteamine-HCl (Sigma; 31 mg/100 g) was administered thrice on day 1 to both groups of animals. Candida albicans at a density of 10(8) in 0.5 mL of saline was administered 1 h before, and 12 h and 24 h after the first administration of cysteamine in the Candida group. RESULTS: Perforated duodenal ulcers were observed in 94.1% of the rats in the Candida group, but only 26.7% of the rats in the saline group (P < 0.01). The area of the duodenal ulcers in the Candida group was 40.89 +/- 33.07 mm2, whereas that in the saline group was 16.53 +/- 20.4 mm2 (P < 0.05). The mortality rate was significantly higher in the Candida group than in the saline group. In the Candida group, colonization by C. albicans was recognized at the ulcer base, surrounded by marked granulocytic infiltration. The number of eosinophils infiltrating the ulcer base was also significantly greater in the Candida group than in the saline group. Immunohistochemical analysis revealed the expression of secretory aspartyl protease (SAP) in the region of the ulcer showing colonization by C. albicans in the Candida group. CONCLUSION: Candida albicans aggravates duodenal ulcer perforation in the experimental model of cysteamine-induced duodenal ulcer perforation. The present findings suggest that SAP and host-parasite relationships, including granulocyte-dependent mechanisms, may be involved in the aggravation of ulcer perforation by C. albicans.


Subject(s)
Candida albicans/isolation & purification , Candidiasis/complications , Duodenal Ulcer/complications , Duodenum/microbiology , Peptic Ulcer Perforation/etiology , Animals , Aspartic Acid Endopeptidases/metabolism , Candida albicans/enzymology , Candidiasis/enzymology , Candidiasis/microbiology , Candidiasis/pathology , Cysteamine , Duodenal Ulcer/chemically induced , Duodenal Ulcer/enzymology , Duodenal Ulcer/pathology , Duodenum/enzymology , Duodenum/pathology , Enzyme-Linked Immunosorbent Assay , Eosinophils/microbiology , Granulocytes/microbiology , Immunohistochemistry , Male , Peptic Ulcer Perforation/enzymology , Peptic Ulcer Perforation/microbiology , Peptic Ulcer Perforation/pathology , Rats , Rats, Wistar , Time Factors
2.
Arq. gastroenterol ; 40(4): 233-238, out.-dez. 2003. tab
Article in Portuguese | LILACS | ID: lil-359884

ABSTRACT

RACIONAL: Várias doenças abdominais podem cursar com aumento de amilasemia e lipasemia. OBJETIVO: Avaliar a validade da amilasemia e lipasemia para os diagnósticos diferenciais entre pancreatite aguda/pancreatite crônica agudizada, doenças das vias biliares, úlcera gastroduodenal perfurada e apendicite aguda. PACIENTES E MÉTODOS: Foram avaliados, prospectivamente, 38 pacientes com pancreatite aguda/pancreatite crônica agudizada, 35 com doenças das vias biliares, 17 com úlcera gastroduodenal perfurada e 44 com apendicite aguda, com idade média (desvio padrão) de 42,4 ± 17,7, 46,7 ± 18,3, 47,8 ± 12 e 33,7 ± 17,8 anos, respectivamente. A amilasemia e a lipasemia foram determinadas à admissão no pronto-socorro. RESULTADOS: Para o diagnóstico de pancreatite aguda/pancreatite crônica agudizada, quando o nível de corte da amilasemia variou entre o limite superior de referência e 5 vezes este limite, a sensibilidade diminuiu de 92 por cento para 74 por cento, a especificidade aumentou de 85 por cento para 99 por cento, o valor preditivo positivo aumentou de 71 por cento para 97 por cento e o valor preditivo negativo diminuiu de 96 por cento para 91 por cento. Para a lipasemia valores semelhantes foram obtidos para sensibilidade e valor preditivo negativo, mas a especificidade e o valor preditivo positivo foram mais baixos. Quando se considerou amilasemia ou lipasemia elevadas, houve pequeno aumento na sensibilidade e no valor preditivo negativo. CONCLUSÕES: Para o diagnóstico de pancreatite aguda/pancreatite crônica agudizada, 1) o melhor nível de corte para ambos os testes foi o de duas vezes o limite superior de referência; 2) as sensibilidades da amilasemia e da lipasemia foram semelhantes; 3) a especificidade e o valor preditivo positivo da amilasemia foram ligeiramente maiores do que as da lipasemia; 4) a sensibilidade, mas não a especificidade, aumentou quando pelo menos uma das enzimas estava elevada.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Abdominal Pain/diagnosis , Abdominal Pain/enzymology , Amylases/blood , Lipase/blood , Pancreatitis/diagnosis , Pancreatitis/enzymology , Acute Disease , Abdominal Pain/etiology , Appendicitis/complications , Appendicitis/diagnosis , Appendicitis/enzymology , Bile Duct Diseases/complications , Bile Duct Diseases/diagnosis , Bile Duct Diseases/enzymology , Chronic Disease , Diagnosis, Differential , Prospective Studies , Pancreatitis/complications , Peptic Ulcer Perforation/complications , Peptic Ulcer Perforation/diagnosis , Peptic Ulcer Perforation/enzymology , Sensitivity and Specificity
3.
Lik Sprava ; (6): 47-9, 1998 Aug.
Article in Russian | MEDLINE | ID: mdl-9844870

ABSTRACT

On the basis of investigations designed to study the condition of intragastric proteolysis and membrane digestion in the small intestine in 148 patients it has been found out that peptic ulcer is accompanied by enhancement of digestive processes in the stomach and by decrease thereof in the small intestine. With the development of peptic ulcer complications digestive disorders get aggravated, which fact leads to various manifestations of the enteral syndrome. A conclusion has been reached that study into intragastric proteolysis and membrane digestion can be useful in the objective evaluation of the activity and degree of severity of the ulcerous process as well as in the functional assessment of results of the treatments administered.


Subject(s)
Digestion , Duodenal Ulcer/complications , Intestine, Small/enzymology , Peptic Ulcer Hemorrhage/enzymology , Peptic Ulcer Perforation/enzymology , Pyloric Stenosis/enzymology , Stomach/enzymology , Adult , Aged , Chronic Disease , Duodenal Ulcer/enzymology , Humans , Hydrogen-Ion Concentration , Middle Aged , Peptide Hydrolases/metabolism
4.
Vrach Delo ; (10): 20-2, 1989 Oct.
Article in Russian | MEDLINE | ID: mdl-2694608

ABSTRACT

Investigation of the state of intraventricular proteolysis and membraneous digestion in the small intestine in 148 patients revealed that ulcer disease is accompanied by potentiation of the digestive processes in the stomach and their reduction in the small intestine. With appearance of complications of ulcer disease, the disorders of digestion deteriorated. This resulted in different manifestations of the enteral syndrome. It is concluded that examination of the intraventricular proteolysis and membraneous digestion may be used for objective evaluation of the activity and grade of severity of the ulcer process as well as for functional evaluation of treatment results.


Subject(s)
Digestion/physiology , Duodenal Ulcer/complications , Intestine, Small/enzymology , Peptic Ulcer Hemorrhage/enzymology , Peptic Ulcer Perforation/enzymology , Pyloric Stenosis/enzymology , Stomach/enzymology , Adult , Aged , Chronic Disease , Duodenal Ulcer/enzymology , Humans , Middle Aged , Peptide Hydrolases/metabolism
6.
Minerva Chir ; 36(10): 655-60, 1981 May 31.
Article in Italian | MEDLINE | ID: mdl-6166897

ABSTRACT

It is often difficult to differentiate acute pancreatitis (A.P.) from some other acute abdominal diseases, when there is an elevated serum amylase. In contrast, the renal clearance of amylase, expressed as a percentage of creatinine clearance, can separate patients with A.P. from patients with acute colecistitis, common duct stone without pancreatitis, hyperamylasemia after biliary surgery, acute peptic ulcer and acute salivary diseases.


Subject(s)
Amylases/blood , Pancreatitis/enzymology , Acute Disease , Cholecystitis/enzymology , Cholestasis, Extrahepatic/enzymology , Gallstones/enzymology , Humans , Peptic Ulcer/enzymology , Peptic Ulcer Perforation/enzymology , Sialadenitis/enzymology
8.
S Afr Med J ; 50(40): 1559-61, 1976 Sep 18.
Article in English | MEDLINE | ID: mdl-982210

ABSTRACT

The amylase:creatinine clearance ratio in patients suffering from acute pancreatitis or acute duodenal perforation was higher than normal in both groups of patients. These findings cast doubt on the value of this parameter as a specific index of acute pancreatitis. The mechanism or mechanisms underlying the increased amylase excretion have not been determined. However, the markedly elevated urinary excretion of lysozyme observed in some patients suggests, by analogy, that diminished tubular reabsorption of amylase may contribute towards the elevated amylase:creatinine ratio.


Subject(s)
Amylases/metabolism , Creatinine/metabolism , Duodenal Ulcer/complications , Muramidase/urine , Pancreatitis/metabolism , Peptic Ulcer Perforation/metabolism , Acute Disease , Female , Humans , Male , Metabolic Clearance Rate , Pancreatitis/enzymology , Peptic Ulcer Perforation/enzymology
SELECTION OF CITATIONS
SEARCH DETAIL
...