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1.
Biochemistry ; 42(49): 14386-96, 2003 Dec 16.
Article in English | MEDLINE | ID: mdl-14661949

ABSTRACT

The effects of P/P- and P/E-site tRNA(Phe) binding on the 16S rRNA structure in the Escherichia coli 70S ribosome were investigated using UV cross-linking. The identity and frequency of 16S rRNA intramolecular cross-links were determined in the presence of deacyl-tRNA(Phe) or N-acetyl-Phe-tRNA(Phe) using poly(U) or an mRNA analogue containing a single Phe codon. For N-acetyl-Phe-tRNA(Phe) with either poly(U) or the mRNA analogue, the frequency of an intramolecular cross-link C967 x C1400 in the 16S rRNA was decreased in proportion to the binding stoichiometry of the tRNA. A proportional effect was true also for deacyl-tRNA(Phe) with poly(U), but the decrease in the C967 x C1400 frequency was less than the tRNA binding stoichiometry with the mRNA analogue. The inhibition of the C967 x C1400 cross-link was similar in buffers with, or without, polyamines. The exclusive participation of C967 with C1400 in the cross-link was confirmed by RNA sequencing. One intermolecular cross-link, 16S rRNA (C1400) to tRNA(Phe)(U33), was made with either poly(U) or the mRNA analogue. These results indicate a limited structural change in the small subunit around C967 and C1400 during tRNA P-site binding sensitive to the type of mRNA that is used. The absence of the C967 x C1400 cross-link in 70S ribosome complexes with tRNA is consistent with the 30S and 70S crystal structures, which contain tRNA or tRNA analogues; the occurrence of the cross-link indicates an alternative arrangement in this region in empty ribosomes.


Subject(s)
Nucleic Acid Conformation , RNA, Ribosomal, 16S/chemistry , RNA, Transfer, Phe/chemistry , Ribosomes/chemistry , Acetylation/radiation effects , Binding Sites/radiation effects , Cytosine/chemistry , Cytosine/radiation effects , Escherichia coli/chemistry , Escherichia coli/genetics , Escherichia coli/radiation effects , Nucleic Acid Conformation/radiation effects , Peptide Chain Elongation, Translational/genetics , Peptide Chain Elongation, Translational/radiation effects , Photochemistry , Poly U/chemistry , Poly U/genetics , RNA, Bacterial/chemistry , RNA, Bacterial/genetics , RNA, Bacterial/radiation effects , RNA, Messenger/chemistry , RNA, Messenger/genetics , RNA, Messenger/radiation effects , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 16S/radiation effects , RNA, Transfer, Phe/genetics , RNA, Transfer, Phe/radiation effects , Ribosomes/genetics , Ribosomes/radiation effects , Transcription, Genetic/radiation effects , Ultraviolet Rays
2.
EMBO J ; 20(20): 5587-94, 2001 Oct 15.
Article in English | MEDLINE | ID: mdl-11598002

ABSTRACT

Absorption of excess light energy by the photosynthetic machinery results in the generation of reactive oxygen species (ROS), such as H2O2. We investigated the effects in vivo of ROS to clarify the nature of the damage caused by such excess light energy to the photosynthetic machinery in the cyanobacterium Synechocystis sp. PCC 6803. Treatments of cyanobacterial cells that supposedly increased intracellular concentrations of ROS apparently stimulated the photodamage to photosystem II by inhibiting the repair of the damage to photosystem II and not by accelerating the photodamage directly. This conclusion was confirmed by the effects of the mutation of genes for H2O2-scavenging enzymes on the recovery of photosystem II. Pulse labeling experiments revealed that ROS inhibited the synthesis of proteins de novo. In particular, ROS inhibited synthesis of the D1 protein, a component of the reaction center of photosystem II. Northern and western blot analyses suggested that ROS might influence the outcome of photodamage primarily via inhibition of translation of the psbA gene, which encodes the precursor to D1 protein.


Subject(s)
Cyanobacteria/radiation effects , Light/adverse effects , Photosynthesis/radiation effects , Photosynthetic Reaction Center Complex Proteins/radiation effects , Photosystem II Protein Complex , Reactive Oxygen Species/metabolism , Bacterial Proteins/biosynthesis , Bacterial Proteins/genetics , Blotting, Northern , Blotting, Western , Cyanobacteria/drug effects , Cyanobacteria/genetics , Cyanobacteria/metabolism , Gene Expression Regulation, Bacterial/radiation effects , Genes, Bacterial , Hydrogen Peroxide/metabolism , Hydrogen Peroxide/toxicity , Oxidative Stress , Paraquat/toxicity , Peptide Chain Elongation, Translational/radiation effects , Peptide Chain Initiation, Translational/radiation effects , Photosynthetic Reaction Center Complex Proteins/biosynthesis , Photosynthetic Reaction Center Complex Proteins/genetics , Photosynthetic Reaction Center Complex Proteins/metabolism , RNA, Bacterial/biosynthesis , RNA, Bacterial/genetics , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Radiation-Sensitizing Agents/toxicity
4.
Eur J Biochem ; 257(1): 78-84, 1998 Oct 01.
Article in English | MEDLINE | ID: mdl-9799105

ABSTRACT

Intact and lysed chloroplasts isolated from the day or night phase of seedling growth exhibit a higher rate of [35S]Met incorporation into the D1 protein in the light than in darkness. In the presence of the translation initiation inhibitor lincomycin, radiolabel incorporation remains unaffected for 7.5-10 min of the in vitro translation reaction, indicating that radiolabel incorporation is regulated by translation elongation. The rate of [35S]Met incorporation into D1-protein can be increased by addition of exogenous ATP to the in vitro translation reactions; however, ATP cannot replace light, and at physiological concentrations of stromal ATP (40 microM), the rate is at least 25-fold higher in the light than in darkness. This indicates that translation elongation is arrested in darkness. Separation of translation-elongation reactions into polysome-bound and membrane-integrated D1 proteins demonstrates that the rate of translation elongation is higher in the presence of light. In the light, less time is required to transiently radiolabel a D1 translation intermediate of about 17 kDa and to chase the translation intermediate into mature D1 protein. We propose that light regulates the enzymatic activity of the translation-elongation process in chloroplasts.


Subject(s)
Chloroplasts/radiation effects , Light , Peptide Chain Elongation, Translational/radiation effects , Photosynthetic Reaction Center Complex Proteins/genetics , Chloroplasts/metabolism , Kinetics , Methionine/metabolism , Photosynthetic Reaction Center Complex Proteins/metabolism , Photosystem II Protein Complex , Sulfur Radioisotopes
5.
J Biol Chem ; 273(33): 20935-40, 1998 Aug 14.
Article in English | MEDLINE | ID: mdl-9694842

ABSTRACT

Upon transfer of lysed chloroplasts from darkness to light, the accumulation of membrane and stromal chloroplast proteins is strictly regulated at the level of translation elongation. In darkness, translation elongation is retarded even in the presence of exogenously added ATP and dithiothreitol. In the light, addition of the electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethyl urea inhibits translation elongation even in the presence of ATP. This inhibition can be overcome by addition of artificial electron donors in the presence of light, but not in darkness. Electron flow between photosystem II and I induced by far red light of 730 nm is sufficient for the activation of translation elongation. This activation can also be obtained by electron donors to photosystem I, which transport protons into the thylakoid lumen. Release of the proton gradient by uncouplers prevents the light-dependent activation of translation elongation. Also, the induction of translation activation is switched off rapidly upon transfer from light to darkness. Hence, we propose that the formation of a photosynthetic proton gradient across the thylakoid membrane activates translation elongation in chloroplasts.


Subject(s)
Chloroplasts/radiation effects , Hordeum/radiation effects , Light , Peptide Chain Elongation, Translational/radiation effects , Photosynthesis , Chloroplasts/metabolism , Diuron/pharmacology , Hordeum/metabolism , Peptide Chain Elongation, Translational/drug effects , Protons
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