Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 914
Filter
1.
Sci Rep ; 14(1): 13739, 2024 06 14.
Article in English | MEDLINE | ID: mdl-38877111

ABSTRACT

The study aimed to develop a quantitative colorimetric loop-mediated isothermal amplification technique using the phenol red indicator (QLAMP-PhR) for detecting Fusobacterium nucleatum (Fn) levels in colorectal cancer (CRC) patients and healthy individuals. QLAMP-PhR assays were conducted on 251 stool samples specific for the Fn FadA gene. Six primers were synthesized and utilized with master mix reagents, and a phenol red indicator was employed to enhance the QLAMP-PhR technique. A standard quantitative analysis curve was generated using a logarithmic function (absorbance vs. concentration) by serially diluting the copy number of genomic DNA templates (Fn ATCC25586). The CRC group exhibited a significantly higher abundance of Fn compared to the healthy control group (P < 0.001). These findings suggest that the QLAMP-PhR technique effectively identifies Fn specifically by its gene for the key virulence factor FadA. Additionally, ideas for developing a real-time QLAMP-PhR test were presented. Compared to the traditional polymerase chain reaction (PCR) technique, QLAMP-PhR offers several advantages including rapidity, simplicity, specificity, sensitivity, and cost-effectiveness method that can quantitatively screen for Fn presence in normal populations. The QLAMP-PhR method represents a sensitive and specific amplification assay for the rapid detection of the Fn pathogen. To the best of our knowledge, this study is the first to report the application of QLAMP-PhR for detecting FadA in Fn.


Subject(s)
Colorectal Neoplasms , Colorimetry , Feces , Fusobacterium nucleatum , Nucleic Acid Amplification Techniques , Humans , Colorectal Neoplasms/microbiology , Colorectal Neoplasms/diagnosis , Colorectal Neoplasms/genetics , Fusobacterium nucleatum/genetics , Fusobacterium nucleatum/isolation & purification , Feces/microbiology , Nucleic Acid Amplification Techniques/methods , Colorimetry/methods , Male , Female , Phenolsulfonphthalein , Molecular Diagnostic Techniques/methods , Middle Aged , Aged , Fusobacterium Infections/microbiology , Fusobacterium Infections/diagnosis , Sensitivity and Specificity , Adult
2.
Chemosphere ; 359: 142230, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38705412

ABSTRACT

This work studied the degradation reaction of sulfonated dyes, indigo carmine, phenol red, and their mixtures by non-thermal plasma (NTP). Interestingly, the degradation rate constant showed a faster process and lower activation energy (Ea) for the dye mixtures than for the degradation reaction of the individual dyes. This unexpected result opened up new opportunities for understanding plasma chemistry and the interaction between reactive species formed by the plasma and the target molecule. As no catalyst or chemical additive was added to the reactor, the decrease in Ea came from a self-synergistic effect (SSE), through the dye molecules fragmentation, which resulted in plasma catalysis. The hypothesis proposed in this work is that oxysulfur (SOx) species are formed by the desulfonation reaction of dyes. The sulfonic groups (SO3) present in the chemical structures of dyes can function as precursors for forming several SOx•- species. Studies based on oxygenated sulfonated species such as SO3•-, SO4•- and SO5•- have been widely applied in advanced oxidative and reductive processes due to their satisfactory efficiency and low cost. Among them, SO4•- is the key reactive species with the best performance in the degradation of pollutants due to its high oxidation potential (E° = 2.60 V). In addition, it is an alternative source of HO• in aqueous media, improving the oxidation reaction. In order to elucidate the SSE, the kinetic process was followed by UV-Vis analysis, and the reactive species, such as alkyl, hydroxyl, and oxy-sulfur radicals were identified by Electron Paramagnetic Resonance. The by-products of the NTP degradation reaction were analyzed by ultrafast liquid chromatography coupled with a mass spectrometer, and a fragmentation route was proposed.


Subject(s)
Coloring Agents , Plasma Gases , Coloring Agents/chemistry , Catalysis , Plasma Gases/chemistry , Oxidation-Reduction , Indigo Carmine/chemistry , Water Pollutants, Chemical/chemistry , Phenolsulfonphthalein/chemistry , Kinetics , Sulfonic Acids/chemistry
3.
Article in English | MEDLINE | ID: mdl-38781808

ABSTRACT

Single-pass intestinal perfusion (SPIP) method is a widely used experimental model to determine the intestinal permeability of drugs. These studies are performed in the presence of a reference standard (metoprolol, MT) and a zero permeability marker (phenol red, PR). Therefore, it is important to develop a validated method for simultaneous determination of the investigated compound along with MT and PR. The aim of this study was to develop a reversed phase high-performance liquid chromatography (RP-HPLC) method with UV-detection for the simultaneous determination of atenolol (ATN), MT, and PR in the perfusion medium used in SPIP experiments. Separation of compounds were performed using an InertSustain C18 (250 × 4.6 mm, 5 µm) HPLC column at 35 °C. The mobile phase was a mixture of acetonitrile and phosphate buffer (pH 7.0, 12.5 mM) in gradient elution, and was delivered at a flow rate of 1 mL/min. The acetonitrile ratio of the mobile phase increased linearly from 10 to 35 % over 15 min. The injection volume was 20 µL, and ATN, MT and PR were detected at 224 nm. The retention times under optimum HPLC conditions were 5.028 min, 12.401 min, and 13.507 min for ATN, MT and PR, respectively. The developed RP-HPLC method was validated for selectivity, specificity, calibration curve and range, accuracy and precision, carry-over effect, stability, reinjection reproducibility, recovery and robustness. The method was linear for ATN (0.76-50 µg/mL), MT (1.14-50 µg/mL), and PR (0.47-20 µg/mL) with determination coefficients of 0.9999, 0.9994 and 0.9998, respectively. The results obtained for all validation parameters of the developed RP-HPLC method met the required limits of the ICH M10 Guideline.


Subject(s)
Atenolol , Chromatography, Reverse-Phase , Metoprolol , Phenolsulfonphthalein , Chromatography, High Pressure Liquid/methods , Animals , Atenolol/analysis , Metoprolol/analysis , Rats , Chromatography, Reverse-Phase/methods , Reproducibility of Results , Linear Models , Phenolsulfonphthalein/chemistry , Male , Limit of Detection , Rats, Wistar , Perfusion
4.
Food Chem ; 451: 139428, 2024 Sep 01.
Article in English | MEDLINE | ID: mdl-38678665

ABSTRACT

For some aquatic products, pH has been considered a useful index to reflect the changes in materials during the loss of freshness. Based on the inner filter effect (IFE) between deprotonated phenol red (PR) and upconversion nanoparticles (UCNPs), UCNPs coated with PR-doped SiO2 shell were embedded in agarose hydrogel to develop a smartphone-assisted method for pH sensing. With the enhancement of pH response using a phase transfer agent (i.e., tetra butyl ammonium hydroxide, TBAH), the proposed senor realized the colorimetric and fluorescence detection of pH in the range of pH 6.6-8 and pH 6-8, respectively. The sensor also showed satisfied reversibility when switched between pH 6 and 8 for at least 5 cycles. Moreover, this sensor displayed great sensitivity, stability, and portability in analyzing actual fish, shrimp, and shellfish samples, providing a new sight for evaluating the freshness of aquatic products.


Subject(s)
Fishes , Hydrogels , Phenolsulfonphthalein , Silicon Dioxide , Smartphone , Hydrogels/chemistry , Hydrogen-Ion Concentration , Silicon Dioxide/chemistry , Animals , Phenolsulfonphthalein/chemistry , Seafood/analysis , Nanoparticles/chemistry , Shellfish/analysis , Colorimetry
5.
Int J Biol Macromol ; 266(Pt 1): 131212, 2024 May.
Article in English | MEDLINE | ID: mdl-38552693

ABSTRACT

In this study, we successfully developed a screen-printed pH-responsive intelligent label using low molecular weight chitosan grafted with phenol red (LCPR) as a colorant for screen printing ink. The LCPR was synthesized via a Mannich reaction, and its successful grafting was confirmed through FT-IR, UV-vis, and NMR spectroscopy. The LCPR exhibited lower crystallinity and thermal stability compared to low molecular weight chitosan (LC) and demonstrated zwitterionic behavior. To create intelligent labels, the LCPR-based ink was efficiently printed on cotton substrates with high resolution. The label exhibited remarkable sensitivity to buffer pH solutions and ammonia gas, leading to distinctive color changes from orange to red to purple. Additionally, the label showed excellent reversibility, storage stability, and leaching resistance to different food simulant solutions. The label was utilized to monitor shrimp freshness, successfully detecting a noticeable color shift upon spoilage. These findings highlight the significant potential of the LCPR-based label as an intelligent food packaging solution, offering pH-responsiveness and color stability for qualitative freshness detection of protein-rich food.


Subject(s)
Chitosan , Food Packaging , Molecular Weight , Phenolsulfonphthalein , Chitosan/chemistry , Food Packaging/methods , Hydrogen-Ion Concentration , Phenolsulfonphthalein/chemistry , Animals
6.
Int J Biol Macromol ; 261(Pt 1): 129042, 2024 Mar.
Article in English | MEDLINE | ID: mdl-38161021

ABSTRACT

The dramatic increase of drug-resistant pathogenic bacteria has seriously effect on human health, appealing the needs of developing theranostic platforms with stimuli-responsive materials to realize the accurate bacterial diagnostics and therapeutics. Herein, a tannic acid and carboxymethyl chitosan-based multifunctional ZIF-90@i-PPOPs-phenol red double-layered hydrogel with stimuli-responsiveness and antibacterial activity was fabricated. The inner layer hydrogel (ZIF-90@i-PPOPs-based TFC hydrogels) was fabricated based on ZIF-90@i-PPOPs, integrate tannic acid and carboxymethyl chitosan linked by formylphenylboronic acid (FPBA), which exhibited outstanding injectable, biodegradability and antibacterial activity. The outer layer hydrogel (PR@PAM hydrogels) were constructed from polyacrylamide (PAM) and pH indicator phenol red, owning porous structure and excellent tissue adhesion. Due to the weakly acidic microenvironment within wound, the inner-layer hydrogel was stimulus-responsively decomposed, resulting in the accurate delivery of the positively charged ZIF-90@i-PPOPs to the lesion site to capture and kill bacteria by enhanced Zn2+ and ROS release. Meantime, the outer-layer hydrogel could real-timely monitor the pH changes to evaluate the wound recovery status. These double-layered hydrogels possessed precisely pH monitoring capacity, excellent antibacterial ability and negligible side effect to normal tissue in vivo, implying the high potential of the suggested hydrogels as theranostic platform for antibacterial treatment.


Subject(s)
Chitosan , Hydrogels , Metal-Organic Frameworks , Nanoparticles , Polyphenols , Humans , Hydrogels/pharmacology , Phenolsulfonphthalein , Anti-Bacterial Agents/pharmacology , Hydrogen-Ion Concentration
7.
BMC Ophthalmol ; 23(1): 498, 2023 Dec 07.
Article in English | MEDLINE | ID: mdl-38062441

ABSTRACT

BACKGROUND: To investigate the validation of phenol red thread (PRT) test in a Chinese population by evaluating the intraobserver repeatability and interobserver reproducibility, determining correlations between the PRT test and other dry eye disease (DED) parameters including tear meniscus height (TMH) and Schirmer I test, and testing the accuracy of diagnosing DED when using the PRT test alone. METHODS: A total of 108 eyes were involved in this prospective and diagnostic study, and were divided into two groups (with and without DED). Each subject underwent a series of ocular surface examinations, including Ocular Surface Disease Index (OSDI) questionnaire, non-invasive tear breakup time (NIBUT), tear meniscus height (TMH) assessment, PRT test, fluorescein tear breakup time (FBUT), corneal fluorescein staining and Schirmer I test. RESULTS: In the experimental group and the control group, the intra-class correlation coefficients (ICCs) of the repeatability were 0.747 and 0.723, respectively (all P < 0.05). The ICCs of the reproducibility in both groups were 0.588 and 0.610, respectively (all P < 0.05). The PRT test correlated weakly with the Schirmer I test and the tear meniscus height, with Spearman coefficients of 0.385 and 0.306, respectively (all P < 0.05). The PRT test is available to diagnose DED, with an area under the curve of 0.806 and a Youden index of 0.556 at the cutoff point of 8.83 mm. CONCLUSIONS: The PRT test can provide patients a comfortable, timesaving and less irritating approach to screening and diagnosing DED compared to Schirmer I test.


Subject(s)
Dry Eye Syndromes , Phenolsulfonphthalein , Humans , Prospective Studies , Reproducibility of Results , Tears , Dry Eye Syndromes/diagnosis , Fluorescein , China
8.
Scand J Clin Lab Invest ; 83(7): 495-500, 2023 Nov.
Article in English | MEDLINE | ID: mdl-37933918

ABSTRACT

Post-natal or newborn screening for thalassemia and hemoglobinopathies is useful for genetic counseling and managing thalassemia in children. We characterized thalassemia genotypes in newborns from the eastern part of Thailand. The results demonstrated a high heterogeneity of thalassemia and hemoglobinopathies with seventeen genotypes. We focused on α0- thalassemia (Southeast Asian [SEA] deletion) in this study. We developed and validated the loop-mediated isothermal amplification (LAMP) colorimetric assay for detecting α0- thalassemia (SEA deletion) using simple direct cord blood sampling compared to genomic DNA. A total of 160 cord blood samples were evaluated with the LAMP assay. The sensitivity and specificity of the LAMP colorimetric assay for α0-thalassemia (SEA deletion) using direct cord blood showed 100% (6/6 x 100) and 98.05% (151/154 x 100) whereas, genomic DNA showed 100% (6/6 x 100) and 100% (154/154 x 100), respectively. Moreover, we demonstrated other simple screening tools for α0-thalassemia with %Hb Bart's, MCV, and MCH values and found that these parameters were not diagnostic in our samples. The direct cord blood with colorimetric LAMP assay is simple, rapid, and does not require a post-LAMP step compared to conventional PCR. These techniques could be applied in post-natal or large population screening for α0-thalassemia (SEA deletion). Finally, this could support early prevention of complications, early management, genetic counseling for α-thalassemia disease in children, or a long-term prevention and control program of severe thalassemia in Thailand.


Subject(s)
Hemoglobinopathies , Hemoglobins, Abnormal , alpha-Thalassemia , Child , Infant, Newborn , Humans , Phenolsulfonphthalein , Colorimetry , Fetal Blood , Thailand , alpha-Thalassemia/diagnosis , alpha-Thalassemia/genetics , Hemoglobins, Abnormal/genetics , DNA
9.
J Pak Med Assoc ; 73(11): 2200-2204, 2023 Nov.
Article in English | MEDLINE | ID: mdl-38013528

ABSTRACT

OBJECTIVE: To assess the tear film parameters in breastfeeding women. satisfaction. Methods: The observational study was conducted at the College of Applied Medical Sciences, Riyadh, Saudi Arabia, from December 15, 2021, to February 12, 2022, and comprised healthy women aged 18-40 years who had no ocular diseases. Breastfeeding women were in group A and non-breastfeeding women formed the control group B. Ocular surface disease index, phenol red thread, and tear ferning tests were used in that order to assess the tear film for all the subjects. A gap of 5 minutes was allowed between phenol red thread and tear ferning tests. Data was analysed using SPSS, version 22. RESULTS: Of the 50 subjects, 25(50%) were in group A with mean age 30.4±5.9 years having a mean breastfeeding period of 5.4±5.0 months. The remaining 25(50%) women were in group B with mean age 28.5±2.1 years. Significant differences were found between the groups for ocular surface disease index, phenol red thread, and tear ferning (p<0.05). Significant moderate correlation was found between tear ferning grades and breastfeeding duration (p<0.05). Conclusion: Breastfeeding was found to increase dry eye symptoms in women.


Subject(s)
Dry Eye Syndromes , Lacerations , Humans , Female , Young Adult , Adult , Male , Breast Feeding , Phenolsulfonphthalein , Dry Eye Syndromes/epidemiology , Tears
10.
Int J Biol Macromol ; 246: 125723, 2023 Aug 15.
Article in English | MEDLINE | ID: mdl-37419265

ABSTRACT

In this study, horseradish peroxidase (HRP) was immobilized for the first time on Ca alginate-starch hybrid beads and employed for the biodegradation of phenol red dye. The optimal protein loading was 50 mg/g of support. Immobilized HRP demonstrated improved thermal stability and maximum catalytic activity at 50 °C and pH 6.0, with an increase in half-life (t1/2) and enzymatic deactivation energy (Ed) compared to free HRP. After 30 days of storage at 4 °C, immobilized HRP retained 109% of its initial activity. Compared to free HRP, the immobilized enzyme exhibited higher potential for phenol red dye degradation, as evidenced by the removal of 55.87% of initial phenol red after 90 min, which was 11.5 times greater than free HRP. In sequential batch reactions, the immobilized HRP demonstrated good potential efficiency for the biodegradation of phenol red dye. The immobilized HRP was used for a total of 15 cycles, degrading 18.99% after 10 cycles and 11.69% after 15 cycles, with a residual enzymatic activity of 19.40% and 12.34%, respectively. Overall, the results suggest that HRP immobilized on Ca alginate-starch hybrid supports shows promise as a biocatalyst for industrial and biotechnological applications, particularly for the biodegradation of recalcitrant compounds such as phenol red dye.


Subject(s)
Phenol , Phenolsulfonphthalein , Horseradish Peroxidase/chemistry , Alginates/chemistry , Phenols , Enzymes, Immobilized/chemistry , Enzyme Stability
11.
Int J Mol Sci ; 24(11)2023 May 30.
Article in English | MEDLINE | ID: mdl-37298425

ABSTRACT

Hormone receptor-positive breast cancer (HR+ BC) cells depend on estrogen and its receptor, ER. Due to this dependence, endocrine therapy (ET) such as aromatase inhibitor (AI) treatment is now possible. However, ET resistance (ET-R) occurs frequently and is a priority in HR+ BC research. The effects of estrogen have typically been determined under a special culture condition, i.e., phenol red-free media supplemented with dextran-coated charcoal-stripped fetal bovine serum (CS-FBS). However, CS-FBS has some limitations, such as not being fully defined or ordinary. Therefore, we attempted to find new experimental conditions and related mechanisms to improve cellular estrogen responsiveness based on the standard culture medium supplemented with normal FBS and phenol red. The hypothesis of pleiotropic estrogen effects led to the discovery that T47D cells respond well to estrogen under low cell density and medium replacement. These conditions made ET less effective there. The fact that several BC cell culture supernatants reversed these findings implies that housekeeping autocrine factors regulate estrogen and ET responsiveness. Results reproduced in T47D subclone and MCF-7 cells highlight that these phenomena are general among HR+ BC cells. Our findings offer not only new insights into ET-R but also a new experimental model for future ET-R studies.


Subject(s)
Breast Neoplasms , Humans , Female , Breast Neoplasms/drug therapy , Estrogens/pharmacology , Aromatase Inhibitors/pharmacology , MCF-7 Cells , Phenolsulfonphthalein/pharmacology
12.
Rev Argent Microbiol ; 55(3): 262-271, 2023.
Article in English | MEDLINE | ID: mdl-37019800

ABSTRACT

Biofilm formation by Bacillus cereus strains is now recognized as a systematic contamination mechanism in foods; the aim of this study was to evaluate the production of submerged and interface biofilms in strains of B. cereus group in different materials, the effect of dextrose, motility, the presence of genes related to biofilms and the enterotoxigenic profile of the strains. We determine biofilm production by safranin assay, motility on semi-solid medium, toxin gene profiling and genes related to biofilm production by PCR in B. cereus group isolated from food. In this study, we observe strains used a higher production of biofilms in PVC; in the BHI broth, no submerged biofilms were found compared to phenol red broth and phenol red broth supplemented with dextrose; no strains with the ces gene were found, the enterotoxin profile was the most common the profile that includes genes for the three enterotoxins. We observed a different distribution of tasA and sipW with the origin of isolation of the strain, being more frequent in the strains isolated from eggshell. The production and type of biofilms are differential according to the type of material and culture medium used.


Subject(s)
Bacillus , Bacillus cereus/genetics , Phenolsulfonphthalein/analysis , Enterotoxins/genetics , Enterotoxins/analysis , Food Microbiology , Biofilms , Glucose
13.
Environ Monit Assess ; 195(5): 574, 2023 Apr 15.
Article in English | MEDLINE | ID: mdl-37060479

ABSTRACT

Water shortage is considered as one of the main challenges of human life. A practical solution to this problem is the wastewater treatment. The removal of dyes from wastewaters has received considerable critical attention by researchers due to their high volume and toxicity. In the current research, the adsorption of phenol red dyes from synthetic wastewater using the activated carbon produced from Mespilus germanica modified with Fe2(MoO4)3 was studied. The proposed adsorbent was characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), energy dispersive X-ray analysis (EDX)/Map, Brunauer-Emmett-Teller (BET), and Raman techniques. The optimal adsorption operating parameters including pH, stirring rate, temperature, dosage of adsorbent, dye initial concentration, and contact time were 3, 500 rpm, 25 °C, 1 g/L, 10 mg/L, and 60 min, respectively. Furthermore, the successful regeneration of the adsorbent for 3 times, using methanol solution as a regeneration medium, denoted its capability in performing adsorption and desorption processes. Equilibrium studies showed that the adsorption of phenol red dyes by activated carbon (AC)/Fe2(MoO4)3 was desirable and physical and the experimental data were fitted well by the Freundlich model. In addition, the kinetic behavior of the current adsorption process was well described by the pseudo-second-order kinetic model, while thermodynamic calculations showed that the process was exothermic and spontaneous.


Subject(s)
Wastewater , Water Pollutants, Chemical , Humans , Phenolsulfonphthalein/analysis , Coloring Agents/analysis , Charcoal/chemistry , Adsorption , Water Pollutants, Chemical/analysis , Spectroscopy, Fourier Transform Infrared , Hydrogen-Ion Concentration , Environmental Monitoring , Thermodynamics , Kinetics
14.
Int J Mol Sci ; 24(3)2023 Jan 26.
Article in English | MEDLINE | ID: mdl-36768764

ABSTRACT

Exploring new and high efficiency mimic enzymes is a vital and novel strategy for antibacterial application. Haloperoxidase-like enzymes have attracted wide attention thanks to their amazing catalytic property for hypohalous acid generation from hydrogen peroxide and halides. However, few materials have displayed halogenating catalytic performance until now. Herein, we synthesized N-doped C/CeO2 (N-C/CeO2) composite materials by a combination of the liquid and solid-state method. N-C/CeO2 can possess haloperoxidase-like catalytic activity by catalyzing the bromination of organic signaling compounds (phenol red) with H2O2 at a wide range of temperatures (20 °C to 55 °C), with a solution color changing from yellow to blue. Meanwhile, it exhibits high catalytic stability/recyclability in the catalytic reaction. The synthesized N-C/CeO2 composite can effectively catalyze the oxidation of Br- with H2O2 to produce HBrO without the presence of phenol red. The produced HBrO can resist typical marine bacteria like Pseudomonas aeruginosa. This study provides an efficient biomimetic haloperoxidase and a novel sustainable method for antibacterial application.


Subject(s)
Hydrogen Peroxide , Phenolsulfonphthalein , Carbon , Biomimetics , Oxidation-Reduction
15.
Mikrochim Acta ; 190(2): 79, 2023 01 31.
Article in English | MEDLINE | ID: mdl-36719487

ABSTRACT

The potential of coordination polymers (CPs) as a host of integrating multiple guest species to construct a fluorescence resonance energy transfer (FRET) nanoprobe was demonstrated. The ZnCPs built from zinc(II) and adenine was employed as a model of CPs to integrate carbon dot (CD) and phenol red (PR) for producing the FRET nanoprobe (CD/PR@ZnCPs). Benefiting from the confinement effect of ZnCPs, the integrated CD and PR can be brought in close proximity to favor the occurrence of FRET process from CD to PR, which leads to the quenching of CD fluorescence. However, the FRET process was disrupted upon the red-shift of PR absorption from 428 to 562 nm in alkaline medium, and consequently switches on the fluorescence of CD/PR@ZnCPs. Based on this finding, by utilizing urease to hydrolyze urea and mediate medium pH, a turn-on fluorescent method was established for the detection of urease activity. This fluorescent method has a linear response that covers 5 to 150 U/L urease with a detection limit of 0.74 U/L and exhibits an excellent selectivity over other enzymes. The successful determination of urease in saliva samples demonstrates the applicability of the fluorescent nanoprobe in complex biological matrix.


Subject(s)
Phenolsulfonphthalein , Urease , Carbon , Limit of Detection , Polymers , Nanostructures
16.
Nutrients ; 15(2)2023 Jan 16.
Article in English | MEDLINE | ID: mdl-36678340

ABSTRACT

We studied the activities of Siraitia grosvenorii extracts (SGE) on airway inflammation in a mouse model of chronic obstructive pulmonary disease (COPD) stimulated by cigarette smoke extract (CSE) and lipopolysaccharide (LPS), as well as in LPS-treated human bronchial epithelial cell line (BEAS-2B). SGE improved the viability of LPS-incubated BEAS-2B cells and inhibited the expression and production of inflammatory cytokines. SGE also attenuated the mitogen-activated protein kinase (MAPK)-nuclear factor-kappa B (NF-κB) signaling activated by LPS stimulation in BEAS-2B cells. In mice stimulated by CSE and LPS, we observed the infiltration of immune cells into the airway after COPD induction. SGE reduced the number of activated T cells, B cells, and neutrophils in bronchoalveolar fluid (BALF), lung tissue, mesenteric lymph node, and peripheral blood mononuclear cells, as well as inhibited infiltration into organs and mucus production. The secretion of cytokines in BALF and the expression level of pro-inflammatory cytokines, mucin 5AC, Transient receptor potential vanilloid 1, and Transient receptor potential ankyrin 1 in lung tissue were alleviated by SGE. In addition, to investigate the activity of SGE on expectoration, we evaluated phenol red secretions in the trachea of mice. SGE administration showed the effect of improving expectoration through an increase in phenol red secretion. Consequently, SGE attenuates the airway inflammatory response in CSE/LPS-stimulated COPD. These findings indicate that SGE may be a potential herbal candidate for the therapy of COPD.


Subject(s)
Cigarette Smoking , Pulmonary Disease, Chronic Obstructive , Mice , Humans , Animals , Lipopolysaccharides/pharmacology , Cigarette Smoking/adverse effects , Disease Models, Animal , Leukocytes, Mononuclear/metabolism , Phenolsulfonphthalein/metabolism , Pulmonary Disease, Chronic Obstructive/metabolism , Lung/pathology , Inflammation/metabolism , Cytokines/metabolism , Nicotiana
17.
Acta Biomater ; 155: 292-303, 2023 01 01.
Article in English | MEDLINE | ID: mdl-36435439

ABSTRACT

The management of wound infection remains the major challenges in real-time diagnosis, effective bacterial elimination and rapid wound healing. Herein, we developed injectable theranostic hydrogels to achieve long-term visual imaging of infected wounds and possible infection recurrence and to launch an on-demand bactericidal effect without using any antibiotics. Antimicrobial peptide ε-polylysine (ePL)-derived hydrogels were prepared through the copolymerization of methacrylated ePL (mPL) and the conjugates with tetrakis(4-carboxyphenyl) porphyrin (mPL-TCPP) and phenol red (mPL-Pr). Light illumination of mPL-TCPP produces reactive oxidative species (ROS) to initiate free radical crosslinking into PL@Pr-TCPP hydrogels without using any additional photoinitiators and concurrently exhibits antibacterial photodynamic therapy (PDT). PL@Pr-TCPP hydrogels experience quick color changes from yellow to orange and finally to red when pH values change from 5.0 to 9.0. The actual pH and related bacterial levels in the wounds could be read from G/B signal ratios of hydrogel colors captured by a smart phone. The conjugation of phenol red and TCPP into hydrogels affords a robust bacterial infection diagnosis and persistent bactericidal effect after cycled light illumination. The bacterial capture by ePL hydrogels strengthens PDT effect through alleviating the short lifetime and action distance of ROS. On a Staphylococcus aureus-infected abscess model, light illumination of the pregel solutions achieves in situ formation of hydrogel dressings. The synergistic bactericidal performance significantly relieves inflammatory status, accelerates collagen deposition, and promotes neovascularization, leading to full recovery of the infected wounds with regeneration of skin accessories. PL@Pr-TCPP hydrogels on the wound bed show color changes upon the recurrence of bacterial infection, which could also be totally eliminated after light illumination. Therefore, this study demonstrates a feasible strategy to develop theranostic hydrogel dressings for life-cycle diagnosis and on-demand treatment of wound infections. STATEMENT OF SIGNIFICANCE: Over 30% of skin and soft tissue infections become chronic even after appropriate antibacterial treatment, and recurrent infections are commonly reported after initial infection. Challenges remain in the development of theranostic wound dressings having the capability of point-of-care diagnosis, life-cycle monitoring and on-demand elimination of bacterial infection. Herein, light-triggered gelation is used to develop theranostic hydrogels for reversible naked-eye diagnosis and on-demand photodynamic therapy of wound infections. Light illumination plays a "one-stone-two-birds" role, i.e., photodynamically produced reactive oxidative species enable bactericidal effect without using any antibiotics, and the generated free radicals initiate crosslinking of hydrogels without using any additional photoinitiators. Bacterial infection-activated color changes of hydrogels could be captured with a smart phone for on-site and persistent monitoring of bacterial infection and wound healing process.


Subject(s)
Photochemotherapy , Staphylococcal Infections , Wound Infection , Humans , Hydrogels/pharmacology , Hydrogels/therapeutic use , Abscess , Precision Medicine , Reactive Oxygen Species , Phenolsulfonphthalein , Staphylococcal Infections/drug therapy , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/therapeutic use , Bacteria
18.
Zhongguo Zhong Yao Za Zhi ; 47(22): 6191-6198, 2022 Nov.
Article in Chinese | MEDLINE | ID: mdl-36471944

ABSTRACT

This study aims to explore the effect of Sini Decoction on Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB) signaling pathway in the mice with allergic asthma(AA). Forty-eight SPF-grade BALB/c mice were randomly assigned into a blank control group, a model group, a dexamethasone group, and high-, medium-, and low-dose Sini Decoction groups, with 8 mice in each group. The sensitization solution made of ovalbumin and aluminum hydroxide powder was injected intraperitoneally in other groups except the blank control group which was injected with an equal volume of normal saline. The solution(or normal saline) was injected three times in total with an interval of 7 days. At the same time of sensitization, external cold stimulation and ice water were administered in a 4 ℃ climate box for 20 min every day. After modeling, the mice in each group were administrated with corresponding drugs by gavage for 3 weeks. At the end of administration, pentobarbital sodium(30 mg·kg~(-1)) was used for anesthesia, and then the samples were collected for the determination of various indexes. The phenol red test was conducted to evaluate tracheal excretion function. The histopathological changes of lung tissue were observed via hematoxylin-eosin(HE) staining. Masson staining was employed to reveal the deposition of blue collagen fibers around bronchi in lung tissue and the area occupied by blue collagen fibers was calculated. Immunofluorescence method was used to measure the expression of bronchial type Ⅰ collagen(Col-Ⅰ) and α-smooth muscle actin(α-SMA). The protein and mRNA levels of TLR4, NF-κB, cysteinyl aspartate specific proteinase-1(caspase-1), and interleukin-13(IL-13) were determined by Western blot and real-time fluorescence quantitative polymerase chain reaction(real-time PCR), respectively. Compared with the model group, Sini Decoction significantly increased the phenol red excretion from trachea, lowered the lung inflammation score, reduced subepithelial collagen deposition, and decreased Col-Ⅰ and α-SMA levels. Furthermore, the decoction down-regulated the protein and mRNA levels of TLR4, NF-κB, caspase-1, and IL-13 in mouse lung tissue. In conclusion, Sini Decoction can improve air remodeling by inhibiting the TLR4/NF-κB signaling pathway.


Subject(s)
Asthma , NF-kappa B , Mice , Animals , NF-kappa B/genetics , NF-kappa B/metabolism , Airway Remodeling , Interleukin-13/pharmacology , Toll-Like Receptor 4/genetics , Saline Solution/pharmacology , Phenolsulfonphthalein/pharmacology , Asthma/drug therapy , Asthma/genetics , Signal Transduction , Mice, Inbred BALB C , RNA, Messenger , Caspases
19.
Zhen Ci Yan Jiu ; 47(12): 1060-7, 2022 Dec 25.
Article in Chinese | MEDLINE | ID: mdl-36571220

ABSTRACT

OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Zusanli" (ST36), "Sanyinjiao" (SP6) and "Liangmen" (ST21) on gastrointestinal motility, blood glucose content and expression of autophagy-related proteins 1 light chain 3 (LC3), p62, phosphatidyli-nositol-3 kinase (PI3K), protein kinase B (Akt), p-Akt and mammalian target protein of rapamycin (mTOR) of interstitial cells of Cajal (ICCs) in the cultured gastric antrum cells in diabetic gastroparesis (DGP) rats, so as to reveal its mechanisms underlying improvement of DGP. METHODS: A total of 45 Sprague Dawley (SD) rats were randomly divided into blank control, model, EA, medication (3-methyladenine, 3-MA) and EA+3-MA groups, with 9 rats in each group. The DGP model was established by intraperitoneal injection of 2% streptozotocin (STZ) combined with high-fat and high sugar diet for 8 weeks. The gastric emptying rate was measured by using gavage of phenol red (to measure the propelling length of the phenol red/total length of small intestine ×100%). The symptom score (mental state, coat color and luster, behavior and activity, stool traits) of rats was observed every week and the blood glucose content was measured by using a glucometer. EA (20 Hz/100 Hz, 2 mA) was applied to unilateral ST36, SP6 and ST21 alternatively for 15 min, once daily, 5 days a week for 3 weeks. Rats of the 3-MA and 3-MA+EA groups received intraperitoneal injection of 3-MA (30 mg·kg-1·d-1, 10 mg/mL), once daily, 5 days a week for 3 weeks. After 15 days' intervention, the rats were operated for gastric emptying rate test, specimen collection, isolation, and culture of primary ICCs. The expression levels of microtubule associated protein LC3, p62, PI3K, Akt, p-Akt and mTOR of ICCs of cultured gastric antrum cells were detected using Western blot, and the number of autophagosomes in ICC of gastric antrum was observed under transmission electron microscope. RESULTS: Compared with the blank control group, the symptom score, blood glucose, and the expression levels of p62, class Ⅰ PI3K, Akt, p-Akt and mTOR proteins were increased significantly (P<0.01), while the gastric emptying rate and ratio of LC3Ⅱ/LC3Ⅰ and the expression level of class Ⅲ PI3K protein were significantly decreased (P<0.05, P<0.01) in the model group. In comparison with the model group, the increase of symptom score, blood glucose, and expression levels of p62, class Ⅰ PI3K, Akt, p-Akt and mTOR proteins and the decrease of gastric empty rate and LC3Ⅱ/LC3Ⅰ ratio and the expression level of class Ⅲ PI3K protein were all reversed in both EA and EA+3-MA groups (P<0.05, P<0.01), rather than in the 3-MA group. In addition, 3-MA also reversed modeling-induced increase of class Ⅰ PI3K, Akt, p-Akt and mTOR proteins expression (P<0.01). No significant differences were found between the EA and EA+3-MA in downregulating the levels of symptom score and blood glucose content, and in upregulating gastric empty rate(P>0.05). The effect of EA was notably superior to that of EA+3-MA in upregulating the ratio of LC3Ⅱ/LC3Ⅰ and the expression level of class Ⅲ PI3K protein, and in downregulating the expression of p62, class Ⅰ PI3K, Akt, p-Akt and mTOR proteins (P<0.05, P<0.01). The findings of transmission electron microscopy showed obvious swelling, breakage of some mitochondrial cristae in the ICC cells of antrum and no autophagosomes in the model group and 3-MA group, which was milder in the damage of mitochondrial cristae and marked increase in the autophagosomes in both EA and EA+3-MA groups. CONCLUSION: EA can improve the gastrointestinal motility and symptoms in DGP rats, which may be related to its functions in downregulating PI3K/Akt/mTOR signaling to promote autophagy level of ICC.


Subject(s)
Diabetic Neuropathies , Electroacupuncture , Gastroparesis , Interstitial Cells of Cajal , Rats , Animals , Rats, Sprague-Dawley , Proto-Oncogene Proteins c-akt/genetics , Proto-Oncogene Proteins c-akt/metabolism , Interstitial Cells of Cajal/metabolism , Phosphatidylinositol 3-Kinases/genetics , Blood Glucose/metabolism , Phenolsulfonphthalein/metabolism , Gastroparesis/genetics , Gastroparesis/therapy , Gastroparesis/metabolism , Signal Transduction , Paresis/metabolism , Pyloric Antrum/metabolism , TOR Serine-Threonine Kinases/genetics , Autophagy , Gastrointestinal Motility , Mammals/metabolism
20.
ACS Appl Mater Interfaces ; 14(46): 52422-52429, 2022 Nov 23.
Article in English | MEDLINE | ID: mdl-36351194

ABSTRACT

Dehydration has always been a confusing problem for a hydrogel exposed to air, which restricts its application in practical detection. While an ionogel with unique properties can lock water molecules efficiently due to its low vapor pressure, the design and development of an ionogel with excellent water-locking properties and stability to achieve satisfactory detection are thus highly desirable. In this work, a pH-sensitive, stretchable, antibacterial, and stable ionogel artificial tongue was fabricated through dual cross-linking. The artificial tongue consisted of MXene as a cross-linking agent, phenol red as an indicator, gelatin, N-(2-hydroxyethyl) acrylamide, and 1-butyl-3-methylimidazolium chloride as the three-dimensional polymer network. A small quantity of MXene was used as a cross-linker the first time to promote the initiator in the system to generate free radicals, accelerating the reaction process and the multilevel linkage among the polymers. Meanwhile, the indicator phenol red was introduced into the ionogel successfully for the first time, showing great stability in 5 weeks. After evaluation by the Brand-Altman analysis, it was found that the method of pH measurement using ionogels had good consistency with the pH meter measurement method. Additionally, the prepared ionogel presented excellent water retention, mechanical stretchability, antibacterial property, and stability, as well as good test results in the test of artificial simulated saliva, demonstrating great potential in clinical applications.


Subject(s)
Phenolsulfonphthalein , Polymers , Anti-Bacterial Agents/pharmacology , Water , Tongue , Hydrogen-Ion Concentration
SELECTION OF CITATIONS
SEARCH DETAIL
...