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1.
Environ Sci Pollut Res Int ; 19(8): 3400-11, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22528988

ABSTRACT

PURPOSE: The aim of this paper was to develop a new recreational water quality index (RWQI) as a tool to ensure the health of swimmers and to take practical decisions. METHODS: RWQI was elaborated with epidemiological data, and we carried out an exhaustive study of the different guidelines for recreational waters proposed by different organisations around the world. Different parameters were chosen, considering, as a priority, the swimmer's contact and the possibility of ingestion of water during the recreational activity. Furthermore, rating curves were established for pH, chemical oxygen demand, nitrate, phosphate, detergents, enterococci, total coliforms, faecal coliforms and Escherichia coli. RESULTS AND CONCLUSIONS: The index was applied to the data set on water quality of the Potrero de los Funes River (San Luis, Argentina), generated during 2 years (2009-2010). Following the RWQI values classification, most of the Potrero de los Funes water samples fell in the good quality range during the study period.


Subject(s)
Water Microbiology/standards , Water Quality/standards , Water/chemistry , Water/standards , Argentina , Biological Oxygen Demand Analysis/standards , Detergents/standards , Environmental Monitoring/methods , Environmental Monitoring/standards , Hydrogen-Ion Concentration , Nitrates/standards , Phosphates/standards , Rivers/chemistry , Rivers/microbiology , Swimming
2.
Anal Biochem ; 324(1): 79-83, 2004 Jan 01.
Article in English | MEDLINE | ID: mdl-14654048

ABSTRACT

This paper describes a rapid and sensitive method to determine inorganic phosphate, even in the presence of labile organic phosphate compounds and large quantities of proteins. The method eliminates the use of sodium arsenite, a highly toxic compound, substituting bismuth citrate for it to stabilize the phosphomolybdic acid complex formed during the interaction of inorganic phosphate and molybdate reduced by ascorbic acid. This method has also been adapted to microplates and has been used to determine the activities of Na/K ATPase and alkaline phosphatase of intestinal basolateral and luminal plasma membranes.


Subject(s)
Cell Membrane/enzymology , Enterocytes/enzymology , Organometallic Compounds/chemistry , Phosphates/analysis , Phosphoric Monoester Hydrolases/analysis , Alkaline Phosphatase/analysis , Animals , Arsenites/chemistry , Arsenites/toxicity , Ascorbic Acid/chemistry , Buffers , Intestinal Mucosa/enzymology , Linear Models , Molybdenum/chemistry , Organophosphorus Compounds/analysis , Phosphates/standards , Phosphoric Acids/chemistry , Sodium Compounds/chemistry , Sodium Compounds/toxicity , Sodium-Potassium-Exchanging ATPase/analysis , Spectrophotometry
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