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1.
Biochem J ; 477(22): 4425-4441, 2020 11 27.
Article in English | MEDLINE | ID: mdl-33141153

ABSTRACT

6-Phosphofructokinase-1-kinase (PFK) tetramers catalyse the phosphorylation of fructose 6-phosphate (F6P) to fructose 1,6-bisphosphate (F16BP). Vertebrates have three PFK isoforms (PFK-M, PFK-L, and PFK-P). This study is the first to compare the kinetics, structures, and transcript levels of recombinant human PFK isoforms. Under the conditions tested PFK-M has the highest affinities for F6P and ATP (K0.5ATP 152 µM; K0.5F6P 147 µM), PFK-P the lowest affinities (K0.5ATP 276 µM; K0.5F6P 1333 µM), and PFK-L demonstrates a mixed picture of high ATP affinity and low F6P affinity (K0.5ATP 160 µM; K0.5F6P 1360 µM). PFK-M is more resistant to ATP inhibition compared with PFK-L and PFK-P (respectively, 23%, 31%, 50% decreases in specificity constants). GTP is an alternate phospho donor. Interface 2, which regulates the inactive dimer to active tetramer equilibrium, differs between isoforms, resulting in varying tetrameric stability. Under the conditions tested PFK-M is less sensitive to fructose 2,6-bisphosphate (F26BP) allosteric modulation than PFK-L or PFK-P (allosteric constants [K0.5ATP+F26BP/K0.5ATP] 1.10, 0.92, 0.54, respectively). Structural analysis of two allosteric sites reveals one may be specialised for AMP/ADP and the other for smaller/flexible regulators (citrate or phosphoenolpyruvate). Correlations between PFK-L and PFK-P transcript levels indicate that simultaneous expression may expand metabolic capacity for F16BP production whilst preserving regulatory capabilities. Analysis of cancer samples reveals intriguing parallels between PFK-P and PKM2 (pyruvate kinase M2), and simultaneous increases in PFK-P and PFKFB3 (responsible for F26BP production) transcript levels, suggesting prioritisation of metabolic flexibility in cancers. Our results describe the kinetic and transcript level differences between the three PFK isoforms, explaining how each isoform may be optimised for distinct roles.


Subject(s)
Gene Expression Regulation, Enzymologic , Phosphofructokinases , Transcription, Genetic , Allosteric Regulation , Fructosephosphates/chemistry , Fructosephosphates/genetics , Fructosephosphates/metabolism , Humans , Isoenzymes/biosynthesis , Isoenzymes/chemistry , Isoenzymes/genetics , Organ Specificity , Phosphofructokinases/biosynthesis , Phosphofructokinases/chemistry , Phosphofructokinases/genetics , Phosphorylation
2.
Lett Appl Microbiol ; 61(6): 555-61, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26370507

ABSTRACT

UNLABELLED: Lactobacillus casei LC2W is an exopolysaccharide(EPS)-producing strain with probiotic effects. The low efficiency and unclear regulation mechanism of EPS biosynthesis have become main constraints for its application in food industry. To investigate the major rate-limiting factors of EPS biosynthesis and to improve its yield, metabolic engineering was applied to this strain. Eight relevant genes related to central metabolism, sugar-nucleotides supply, glycosyltransferase and cofactor engineering were cloned and overexpressed. The results suggested that nox, pfk, rfbB and galT genes were the largest contributors to EPS biosynthesis in this study, which elevated EPS yield by 46·0, 20, 17·4 and 19·6% respectively. Notably, under aerobic condition which was not a suitable condition for lactobacilli to grow in, recombinant strain LC-nox achieved the highest EPS yield of 263·7 mg l(-1) , which was increased by 75% compared to that of the starting strain. The oxygen stress was excluded since the phenomenon was not observed in the control strain under the same condition. Therefore, it was probably that higher NADH oxidase activity led to a decreased NADH availability and reduced lactate concentration, which resulted in the elevation of EPS yield. SIGNIFICANCE AND IMPACT OF THE STUDY: This study contributed to the understanding of EPS biosynthesis in Lact. casei through metabolic engineering and provided a starting point for introducing cofactor engineering into this strain. Overexpression of NADH oxidase was found to have a most significant effect on the EPS production. It is the first report that EPS could be accumulated to such a high level under aerobic condition in lactobacilli. Our results provided a novel strategy for the improvement of EPS production in lactic acid bacteria.


Subject(s)
Lacticaseibacillus casei/genetics , Lacticaseibacillus casei/metabolism , Metabolic Engineering/methods , Multienzyme Complexes/metabolism , NADH, NADPH Oxidoreductases/metabolism , Polysaccharides, Bacterial/biosynthesis , Fermentation , Lactic Acid/metabolism , Molecular Sequence Data , Multienzyme Complexes/biosynthesis , Multienzyme Complexes/genetics , NAD/chemistry , NADH, NADPH Oxidoreductases/biosynthesis , NADH, NADPH Oxidoreductases/genetics , Peptidoglycan Glycosyltransferase/genetics , Peptidoglycan Glycosyltransferase/metabolism , Phosphofructokinases/biosynthesis , Phosphofructokinases/genetics , Probiotics/metabolism , UTP-Hexose-1-Phosphate Uridylyltransferase/biosynthesis , UTP-Hexose-1-Phosphate Uridylyltransferase/genetics
3.
J Assist Reprod Genet ; 32(3): 471-8, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25578537

ABSTRACT

PURPOSE: This study evaluates the effect of low oxygen conditions (5 Vs 20%) on buffalo embryo development. Expression patterns of key glucose metabolism genes (HK, PFK, LDH, PDH, G6PDH and Glut1) were assessed in buffalo oocytes and embryos cultured at 5 and 20% oxygen and correlated with development rate. METHODS: Maturation rate was observed by determining MII stages by Aceto-orcein method and blastocyst formation was observed at 7 day post insemination (dpi). Expression levels of genes were determined by real time PCR in oocytes / embryos at 5 and 20% O2. RESULTS: Oocyte maturation and blastocyst formation rates were significantly higher at 5% O2 as compared to 20% O2 (P < 0.05). The expression pattern of glycolytic genes (HK, PFK and G6PDH) indicated that oocytes and embryos under 5% O2 tend to follow anaerobic glycolysis and pentose phosphate pathways to support optimum embryo development. Under 20% O2, oocytes and embryos had high expression of PDH indicating higher oxidative phosphorylation. Further, less G6PDH expression at 20% O2 was indicative of lower pentose phosphate activity. Higher expression of LDH was observed in oocytes and embryos under 20% O2 indicating sub-optimal culture conditions. High Glut1 activity was observed in the oocytes / embryos at 5% O2, indicative of high glucose uptake correlating with high expression of glycolytic genes. CONCLUSION: The expression patterns of glucose metabolism genes could be a valuable indicator of the development potential of oocytes and embryos. The study indicates the importance of reduced oxygen conditions for production of good quality embryos.


Subject(s)
Embryonic Development/genetics , Glucose , Oocytes/growth & development , Animals , Buffaloes , Female , Fertilization in Vitro , Gene Expression Regulation, Developmental , Glucose/genetics , Glucose/metabolism , Glucose Transporter Type 1/biosynthesis , Glucosephosphate Dehydrogenase/biosynthesis , Hexokinase/biosynthesis , Humans , In Vitro Oocyte Maturation Techniques , Isoenzymes/biosynthesis , L-Lactate Dehydrogenase/biosynthesis , Oocytes/metabolism , Oxygen/metabolism , Phosphofructokinases/biosynthesis , Protein Serine-Threonine Kinases/biosynthesis , Pyruvate Dehydrogenase Acetyl-Transferring Kinase
4.
PLoS One ; 7(9): e44748, 2012.
Article in English | MEDLINE | ID: mdl-22984551

ABSTRACT

The male and the hermaphrodite forms of the nematode Caenorhabditis elegans (C. elegans) differ markedly in anatomy, nervous system and behavior at adulthood. Using the male mutants fog-2, him-5, and him-8, we compared body proportions and composition, and aspects of carbohydrate metabolism and gene expression between the C. elegans sexes in three adult stages. In all experiments, both sexes were grown on the same plate and separated using flow cytometry. The fat to fat-free mass ratio and the body volume-adjusted fat mass is similar between the sexes, although the body size is more than 50% smaller in adult males than in age-matched hermaphrodites. The volume-adjusted total RNA content is approximately 2-fold lower in males. Biochemical and NMR-based analyses reveal higher trehalose levels and much lower glucose levels in males than in hermaphrodites. The resulting trehalose-to-glucose ratio is 5.4-fold higher in males. These sex differences are reflected in gene expression data because the genes encoding key enzymes of the glycolysis and trehalose synthesis pathways are more highly expressed in males than in hermaphrodites. Notably, expression of the phosphofructokinase gene (C50F4.2) is 29-fold higher in males. Comparative analysis of gene expression data identifies 285 male-specific and 160 hermaphrodite-specific genes. These include transcription factor and C-type lectin-encoding genes. More than 35% of all C-type lectin genes are more highly expressed in males. The expression of many C-type lectin genes differs by a factor of >100 between the sexes. In conclusion, we found sex differences in carbohydrate metabolism that are linked to gene expression and identified certain lectin genes that are differentially expressed by the C. elegans sexes.


Subject(s)
Caenorhabditis elegans/metabolism , Carbohydrate Metabolism , Gene Expression Regulation , Animals , Body Size , Female , Flow Cytometry/methods , Green Fluorescent Proteins/metabolism , Lectins, C-Type/metabolism , Male , Phosphofructokinases/biosynthesis , RNA/metabolism , Sex Characteristics , Sex Factors , Trehalose/metabolism
5.
Brain Res ; 1388: 48-55, 2011 May 04.
Article in English | MEDLINE | ID: mdl-21396919

ABSTRACT

The pathophysiology of stroke, a leading cause of morbidity and mortality, is still in the process of being understood. Pre-ischemic exercise has been known to be beneficial in reducing the severity of stroke-induced brain injury in animal models. Forced exercise with a stressful component, rather than voluntary exercise, was better able to induce neuroprotection. This study further determined the changes in cerebral metabolism resulting from the two methods of exercise (forced versus voluntary). Adult male Sprague-Dawley rats were randomly assigned to 3 groups: the control group (no exercise), the forced treadmill exercise group, and the voluntary running wheel exercise group. In order to measure the extent of cerebral metabolism in animals with different exercise regimens, mRNA levels and protein expression of glucose transporter 1 and glucose transporter 3 (GLUT-1 and GLUT-3), phosphofructokinase (PFK), lactate dehydrogenase (LDH), and adenosine monophosphate kinase (AMPK) were measured utilizing real-time reverse transcription polymerase chain reaction (PCR) analysis as well as Western blot analysis. Phosphorylated AMPK activity was also measured using an ELISA activity kit, and hypoxic inducible factor (HIF)-1α was measured at transcription and translation levels. The data show that the forced exercise group had a significant (p < 0.05) increase in cerebral glycolysis, including expressions of GLUT-1, GLUT-3, PFK, LDH, phosphorylated AMPK activity and HIF-1α, when compared to the voluntary exercise and the control groups. Our results suggest that the effects of different exercise on HIF-1α expression and cerebral glycolysis may provide a possible reason for the discrepancy in neuroprotection, with forced exercise faring better than voluntary exercise through increased cerebral metabolism.


Subject(s)
Cerebral Cortex/metabolism , Physical Conditioning, Animal/methods , Animals , Blotting, Western , Glucose Transporter Type 1/biosynthesis , Glucose Transporter Type 3/biosynthesis , Glycolysis/physiology , Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis , L-Lactate Dehydrogenase/biosynthesis , Male , Phosphofructokinases/biosynthesis , RNA, Messenger/analysis , Rats , Rats, Sprague-Dawley , Reverse Transcriptase Polymerase Chain Reaction
6.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 45(10): 627-30, 2010 Oct.
Article in Chinese | MEDLINE | ID: mdl-21176602

ABSTRACT

OBJECTIVE: To investigate the effects of estrogen on the expression of phosphofructokinase muscle-specific isoform (PFK-M) in genioglossus of chronic intermittent hypoxia (CIH) rats. METHODS: Fifty male SD rats were randomly divided into five groups: the normal control group (NC), the chronic intermittent hypoxia group (CIH), and three doses of estrogen plus hypoxia groups (LE, ME, HE). Rats in the latter four groups were used to build CIH models (8 h/d, 5 weeks). In the mean time, rats in the latter three groups were injected with three dose levels of estrogen (0.1, 0.2, 0.3 mg/kg), and rats in NC and CIH groups were injected with sterile olive oil as control. At the end of the treatment, the genioglossus was isolated and quickly removed. The mRNA levels of PFK-M were determined by real-time RT-PCR and the protein content of PFK-M was detected by Western blotting analysis. RESULTS: PFK-M mRNA and protein in CIH group (2.144 ± 0.260, 0.875 ± 0.025) were both higher than those (1.000 ± 0.259, 0.413 ± 0.013) in NC group (P < 0.05). The expression of PFK-M mRNA in LE, ME and HE groups were 1.424 ± 0.193, 1.395 ± 0.251 and 1.310 ± 0.094, respectively. The expression of protein in LE, ME and HE groups were 0.638 ± 0.015, 0.576 ± 0.017 and 0.505 ± 0.021, respectively. Compared with CIH group, the expression of PFK-M mRNA and protein in LE, ME and HE groups were all inhibited significantly (P < 0.05). Among the three treatment groups, decreased protein content of PFK-M was observed only in HE group when compared with LE group (P < 0.05), but no significant difference was detected in the expression of PFK-M mRNA. CONCLUSIONS: CIH exposure could increase the expression of PFK-M mRNA and protein in rat genioglossus, while estrogen administration could dose dependently inhibit the overexpression.


Subject(s)
Estrogens/physiology , Hypoxia , Phosphofructokinases/biosynthesis , Tongue/metabolism , Animals , Male , Muscle, Skeletal/metabolism , Protein Isoforms , RNA, Messenger , Rats
7.
Microbiology (Reading) ; 156(Pt 2): 530-542, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19797358

ABSTRACT

Neisseria meningitidis serogroup B is a pathogen that can infect diverse sites within the human host. According to the N. meningitidis genomic information and experimental observations, glucose can be completely catabolized through the Entner-Doudoroff pathway and the pentose phosphate pathway. The Embden-Meyerhof-Parnas pathway is not functional, because the gene for phosphofructokinase (PFK) is not present. The phylogenetic distribution of PFK indicates that in most obligate aerobic organisms, PFK is lacking. We conclude that this is because of the limited contribution of PFK to the energy supply in aerobically grown organisms in comparison with the energy generated through oxidative phosphorylation. Under anaerobic or microaerobic conditions, the available energy is limiting, and PFK provides an advantage, which explains the presence of PFK in many (facultatively) anaerobic organisms. In accordance with this, in silico flux balance analysis predicted an increase of biomass yield as a result of PFK expression. However, analysis of a genetically engineered N. meningitidis strain that expressed a heterologous PFK showed that the yield of biomass on substrate decreased in comparison with a pfkA-deficient control strain, which was associated mainly with an increase in CO(2) production, whereas production of by-products was similar in the two strains. This might explain why the pfkA gene has not been obtained by horizontal gene transfer, since it is initially unfavourable for biomass yield. No large effects related to heterologous expression of pfkA were observed in the transcriptome. Although our results suggest that introduction of PFK does not contribute to a more efficient strain in terms of biomass yield, achievement of a robust, optimal metabolic network that enables a higher growth rate or a higher biomass yield might be possible after adaptive evolution of the strain, which remains to be investigated.


Subject(s)
Neisseria meningitidis, Serogroup B/enzymology , Phosphofructokinases/biosynthesis , Biomass , Cloning, Molecular , Escherichia coli/genetics , Gene Expression Profiling , Metabolic Networks and Pathways , Neisseria meningitidis, Serogroup B/classification , Neisseria meningitidis, Serogroup B/genetics , Phosphofructokinases/genetics , Phylogeny , RNA, Bacterial/genetics
8.
Mol Biochem Parasitol ; 156(2): 162-6, 2007 Dec.
Article in English | MEDLINE | ID: mdl-17767967

ABSTRACT

Entomopathogenic nematode Heterorhabditis bacteriophora infective juveniles (IJs) employ multiple strategies to combat with insect innate immune system after invasion. We employed suppressive subtractive hybridization (SSH) technique to study the gene expression in the IJs upon contact with the insect hemolymph in vitro. We identified two clones having higher expression levels in the IJs than IJs treated with insect hemolymph. The differential expression levels were confirmed by Northern blot hybridization with reference to the constitutive expression level of Heterorhabditis bacteriophora actin2 gene. Clone HbGPS11.C1G02 encoded a phosphofructokinase (PFK) with a 2.5 kb transcript and clone HbGPS11.C4C08 corresponded to a 2.1 kb transcript encoding a protein that had weak similarity to trans-sialidase from Trypanosoma cruzi. The differential expression of PFK in H. bacteriophora IJs before and during recovery process represented a switch from active movement in search of insect hosts to a state of combating insect innate immune system. This first report of H. bacteriophora differential gene expression provides a glimpse at the gene expression profile of H. bacteriophora IJ recovery process.


Subject(s)
Down-Regulation/physiology , Glycoproteins/biosynthesis , Hemolymph/parasitology , Insecta/parasitology , Neuraminidase/biosynthesis , Phosphofructokinases/biosynthesis , Rhabditoidea/genetics , Amino Acid Sequence , Animals , Blotting, Northern , Glycoproteins/genetics , Molecular Sequence Data , Neuraminidase/genetics , Phosphofructokinases/genetics , RNA/biosynthesis , Sequence Alignment , Sequence Analysis, DNA , Sequence Homology , Trypanosoma cruzi/genetics
9.
J Appl Physiol (1985) ; 95(3): 1201-6, 2003 Sep.
Article in English | MEDLINE | ID: mdl-12766179

ABSTRACT

Changes in gene expression during recovery from high-intensity, intermittent, one-legged exercise were studied before and after 5.5 wk of training. Genes related to metabolism, as well as Na+, K+, and pH homeostasis, were selected for analyses. After the same work was performed before and after the training period, several muscle biopsies were obtained from vastus lateralis muscle. In the untrained state, the Na+-K+-ATPase alpha1-subunit mRNA level was approximately threefold higher (P < 0.01) at 0, 1, and 3 h after exercise, relative to the preexercise resting level. After 3-5 h of recovery in the untrained state, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA levels were elevated 13-fold (P < 0.001) and 6-fold (P < 0.01), respectively. However, after the training period, only pyruvate dehydrogenase kinase 4 mRNA levels were elevated (P < 0.05) during the recovery period. No changes in resting mRNA levels were observed as a result of training. In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished.


Subject(s)
Exercise/physiology , Gene Expression Regulation/physiology , Homeostasis/genetics , Physical Fitness/physiology , ATP-Binding Cassette Transporters , Adult , DNA Primers , Gene Expression Regulation/genetics , Humans , Ions/metabolism , KATP Channels , Male , Muscle, Skeletal/cytology , Muscle, Skeletal/metabolism , Muscle, Skeletal/physiology , Phosphofructokinases/biosynthesis , Phosphofructokinases/genetics , Potassium Channels/biosynthesis , Potassium Channels/genetics , Potassium Channels, Inwardly Rectifying , RNA, Messenger/biosynthesis , Reverse Transcriptase Polymerase Chain Reaction , Ribonuclease H/biosynthesis , Ribonuclease H/genetics , Sodium-Potassium-Exchanging ATPase/metabolism
10.
J Periodontal Res ; 36(6): 390-7, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11762875

ABSTRACT

Differential gene expression was investigated in neutrophils stimulated with N-formyl-methionyl-leucyl-phenylalanine using RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP-PCR). The cells were isolated from 3 groups of subjects: patients with generalized aggressive periodontitis (Aggressive-P. n = 6), generalized chronic periodontitis (Chronic-P, n = 6) and healthy controls (H, n = 8). Our results show that 37 genes were upregulated. while 27 genes were down-regulated in all Aggressive-P neutrophils by using RAP-PCR with 45 primer pairs. Reverse transcription-PCR analyses revealed that mRNA levels were significantly different (p<0.05) for heat shock transcription factor 4b (HSF4b) gene. Kruppel-like zinc finger transcription factor 9 (Zf9) and muskelin genes. HSF4b was greater in neutrophils from Aggressive-P compared to groups H and Chronic-P. Zf9 and muskelin genes were lower in Aggressive-P compared to the H groups, but no significant difference was noted compared to the Chronic-P group. The control genes, IL-1beta and VEGF genes, were expressed at a significantly higher level in Aggressive-P and Chronic-P than H (p<.01, p<0.05). In conclusion, the RAP-PCR technique used in this study enabled us to identify 3 Aggressive-P related genes, which had not been reported previously. Neutrophil functions in Aggressive-P patients are suggested to be altered by regulatory factors of the immune system including HSF4b (transcription factor), Zf9 (activator of TGF-beta) and muskelin (cellular adhesion).


Subject(s)
Intercellular Signaling Peptides and Proteins , Periodontitis/genetics , Periodontitis/immunology , Proto-Oncogene Proteins , Adult , Aggressive Periodontitis/genetics , Aggressive Periodontitis/immunology , Aggressive Periodontitis/metabolism , Case-Control Studies , Cell Adhesion Molecules , DNA-Binding Proteins/biosynthesis , DNA-Binding Proteins/genetics , Dual-Specificity Phosphatases , Female , Gene Expression , Heat Shock Transcription Factors , Humans , Intracellular Signaling Peptides and Proteins , Kruppel-Like Factor 6 , Kruppel-Like Transcription Factors , Male , Mitogen-Activated Protein Kinase Phosphatases , Neutrophils/metabolism , Periodontitis/metabolism , Phosphofructokinases/biosynthesis , Phosphofructokinases/genetics , Protein Biosynthesis , Protein Tyrosine Phosphatases/biosynthesis , Protein Tyrosine Phosphatases/genetics , Proteins/genetics , RNA/analysis , Random Amplified Polymorphic DNA Technique , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Trans-Activators/biosynthesis , Trans-Activators/genetics , Transcription Factors/biosynthesis , Transcription Factors/genetics
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