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1.
J Neuroinflammation ; 18(1): 28, 2021 Jan 19.
Article in English | MEDLINE | ID: mdl-33468163

ABSTRACT

BACKGROUND: Inflammation undermines multiple forms of neuroplasticity. Although inflammation and its influence on plasticity in multiple neural systems has been extensively studied, its effects on plasticity of neural networks controlling vital life functions, such as breathing, are less understood. In this study, we investigated the signaling mechanisms whereby lipopolysaccharide (LPS)-induced systemic inflammation impairs plasticity within the phrenic motor system-a major spinal respiratory motor pool that drives contractions of the diaphragm muscle. Here, we tested the hypotheses that lipopolysaccharide-induced systemic inflammation (1) blocks phrenic motor plasticity by a mechanism that requires cervical spinal okadaic acid-sensitive serine/threonine protein phosphatase (PP) 1/2A activity and (2) prevents phosphorylation/activation of extracellular signal-regulated kinase 1/2 mitogen activated protein kinase (ERK1/2 MAPK)-a key enzyme necessary for the expression of phrenic motor plasticity. METHODS: To study phrenic motor plasticity, we utilized a well-characterized model for spinal respiratory plasticity called phrenic long-term facilitation (pLTF). pLTF is characterized by a long-lasting, progressive enhancement of inspiratory phrenic nerve motor drive following exposures to moderate acute intermittent hypoxia (mAIH). In anesthetized, vagotomized and mechanically ventilated adult Sprague Dawley rats, we examined the effect of inhibiting cervical spinal serine/threonine PP 1/2A activity on pLTF expression in sham-vehicle and LPS-treated rats. Using immunofluorescence optical density analysis, we compared mAIH-induced phosphorylation/activation of ERK 1/2 MAPK with and without LPS-induced inflammation in identified phrenic motor neurons. RESULTS: We confirmed that mAIH-induced pLTF is abolished 24 h following low-dose systemic LPS (100 µg/kg, i.p.). Cervical spinal delivery of the PP 1/2A inhibitor, okadaic acid, restored pLTF in LPS-treated rats. LPS also prevented mAIH-induced enhancement in phrenic motor neuron ERK1/2 MAPK phosphorylation. Thus, a likely target for the relevant okadaic acid-sensitive protein phosphatases is ERK1/2 MAPK or its upstream activators. CONCLUSIONS: This study increases our understanding of fundamental mechanisms whereby inflammation disrupts neuroplasticity in a critical population of motor neurons necessary for breathing, and highlights key roles for serine/threonine protein phosphatases and ERK1/2 MAPK kinase in the plasticity of mammalian spinal respiratory motor circuits.


Subject(s)
Inflammation/metabolism , Motor Neurons/enzymology , Neuronal Plasticity/physiology , Phosphoprotein Phosphatases/metabolism , Phrenic Nerve/enzymology , Animals , Inflammation/chemically induced , Lipopolysaccharides/toxicity , Long-Term Potentiation/physiology , MAP Kinase Signaling System , Male , Rats , Rats, Sprague-Dawley , Respiratory Physiological Phenomena
2.
Neuropharmacology ; 184: 108405, 2021 02 15.
Article in English | MEDLINE | ID: mdl-33212114

ABSTRACT

Sudden unexpected death in epilepsy (SUDEP) is a leading cause of death in patients with refractory epilepsy. Centrally-mediated respiratory dysfunction has been identified as one of the principal mechanisms responsible for SUDEP. Seizures generate a surge in adenosine release. Elevated adenosine levels suppress breathing. Insufficient metabolic clearance of a seizure-induced adenosine surge might be a precipitating factor in SUDEP. In order to deliver targeted therapies to prevent SUDEP, reliable biomarkers must be identified to enable prompt intervention. Because of the integral role of the phrenic nerve in breathing, we hypothesized that suppression of phrenic nerve activity could be utilized as predictive biomarker for imminent SUDEP. We used a rat model of kainic acid-induced seizures in combination with pharmacological suppression of metabolic adenosine clearance to trigger seizure-induced death in tracheostomized rats. Recordings of EEG, blood pressure, and phrenic nerve activity were made concomitant to the seizure. We found suppression of phrenic nerve burst frequency to 58.9% of baseline (p < 0.001, one-way ANOVA) which preceded seizure-induced death; importantly, irregularities of phrenic nerve activity were partly reversible by the adenosine receptor antagonist caffeine. Suppression of phrenic nerve activity may be a useful biomarker for imminent SUDEP. The ability to reliably detect the onset of SUDEP may be instrumental in the timely administration of potentially lifesaving interventions.


Subject(s)
Adenosine Kinase/antagonists & inhibitors , Phrenic Nerve/enzymology , Phrenic Nerve/physiopathology , Seizures/enzymology , Seizures/physiopathology , Sudden Unexpected Death in Epilepsy , Adenosine Kinase/metabolism , Animals , Kainic Acid/toxicity , Male , Phrenic Nerve/drug effects , Predictive Value of Tests , Rats , Rats, Wistar , Seizures/chemically induced , Tubercidin/analogs & derivatives , Tubercidin/pharmacology
3.
J Neurosci ; 35(21): 8107-17, 2015 May 27.
Article in English | MEDLINE | ID: mdl-26019328

ABSTRACT

Acute intermittent hypoxia (AIH) induces a form of spinal motor plasticity known as phrenic long-term facilitation (pLTF); pLTF is a prolonged increase in phrenic motor output after AIH has ended. In anesthetized rats, we demonstrate that pLTF requires activity of the novel PKC isoform, PKCθ, and that the relevant PKCθ is within phrenic motor neurons. Whereas spinal PKCθ inhibitors block pLTF, inhibitors targeting other PKC isoforms do not. PKCθ is highly expressed in phrenic motor neurons, and PKCθ knockdown with intrapleural siRNAs abolishes pLTF. Intrapleural siRNAs targeting PKCζ, an atypical PKC isoform expressed in phrenic motor neurons that underlies a distinct form of phrenic motor plasticity, does not affect pLTF. Thus, PKCθ plays a critical role in spinal AIH-induced respiratory motor plasticity, and the relevant PKCθ is localized within phrenic motor neurons. Intrapleural siRNA delivery has considerable potential as a therapeutic tool to selectively manipulate plasticity in vital respiratory motor neurons.


Subject(s)
Hypoxia/enzymology , Isoenzymes/metabolism , Long-Term Potentiation/physiology , Motor Neurons/enzymology , Phrenic Nerve/enzymology , Protein Kinase C/metabolism , Animals , Enzyme Activation/drug effects , Enzyme Activation/physiology , Hypoxia/physiopathology , Isoenzymes/antagonists & inhibitors , Long-Term Potentiation/drug effects , Male , Motor Neurons/drug effects , Phrenic Nerve/drug effects , Protein Kinase C/antagonists & inhibitors , Protein Kinase C-theta , Protein Kinase Inhibitors/pharmacology , Rats , Rats, Sprague-Dawley
4.
Am J Physiol Regul Integr Comp Physiol ; 308(8): R700-7, 2015 Apr 15.
Article in English | MEDLINE | ID: mdl-25673781

ABSTRACT

In many neural networks, mechanisms of compensatory plasticity respond to prolonged reductions in neural activity by increasing cellular excitability or synaptic strength. In the respiratory control system, a prolonged reduction in synaptic inputs to the phrenic motor pool elicits a TNF-α- and atypical PKC-dependent form of spinal plasticity known as inactivity-induced phrenic motor facilitation (iPMF). Although iPMF may be elicited by a prolonged reduction in respiratory neural activity, iPMF is more efficiently induced when reduced respiratory neural activity (neural apnea) occurs intermittently. Mechanisms giving rise to iPMF following intermittent neural apnea are unknown. The purpose of this study was to test the hypothesis that iPMF following intermittent reductions in respiratory neural activity requires spinal TNF-α and aPKC. Phrenic motor output was recorded in anesthetized and ventilated rats exposed to brief intermittent (5, ∼1.25 min), brief sustained (∼6.25 min), or prolonged sustained (30 min) neural apnea. iPMF was elicited following brief intermittent and prolonged sustained neural apnea, but not following brief sustained neural apnea. Unlike iPMF following prolonged neural apnea, spinal TNF-α was not required to initiate iPMF during intermittent neural apnea; however, aPKC was still required for its stabilization. These results suggest that different patterns of respiratory neural activity induce iPMF through distinct cellular mechanisms but ultimately converge on a similar downstream pathway. Understanding the diverse cellular mechanisms that give rise to inactivity-induced respiratory plasticity may lead to development of novel therapeutic strategies to treat devastating respiratory control disorders when endogenous compensatory mechanisms fail.


Subject(s)
Hypocapnia/enzymology , Neuronal Plasticity , Neurons/enzymology , Phrenic Nerve/enzymology , Protein Kinase C/metabolism , Respiratory Center/enzymology , Respiratory Muscles/innervation , Signal Transduction , Spinal Nerves/enzymology , Tumor Necrosis Factor-alpha/metabolism , Action Potentials , Animals , Disease Models, Animal , Hypercapnia/enzymology , Hypercapnia/physiopathology , Hypocapnia/blood , Hypocapnia/physiopathology , Male , Phrenic Nerve/physiopathology , Rats, Sprague-Dawley , Respiratory Center/physiopathology , Spinal Nerves/physiopathology , Time Factors
5.
J Neurosci ; 31(21): 7682-90, 2011 May 25.
Article in English | MEDLINE | ID: mdl-21613481

ABSTRACT

Although vascular endothelial growth factor (VEGFA-165) is primarily known for its role in angiogenesis, it also plays important neurotrophic and neuroprotective roles for spinal motor neurons. VEGFA-165 signals by activating its receptor tyrosine kinase VEGF receptor-2 (VEGFR-2). Because another growth/trophic factor that signals via a receptor tyrosine kinase (brain derived neurotrophic factor) elicits a long-lasting facilitation of respiratory motor activity in the phrenic nerve, we tested the hypothesis that VEGFA-165 elicits similar phrenic motor facilitation (pMF). Using immunohistochemistry and retrograde labeling techniques, we demonstrate that VEGFA-165 and VEGFR-2 are expressed in identified phrenic motor neurons. Furthermore, intrathecal VEGFA-165 administration at C4 elicits long-lasting pMF; intraspinal VEGFA-165 increased integrated phrenic nerve burst amplitude for at least 90 min after injection (53.1 ± 5.0% at 90 min; p < 0.001). Intrathecal VEGFA-165 increased phosphorylation (and presumed activation) of signaling molecules downstream from VEGFR-2 within the phrenic motor nucleus, including ERK (1.53 ± 0.13 vs 1.0 ± 0.05 arbitrary units in control rats; p < 0.05) and Akt (2.16 ± 0.41 vs 1.0 ± 0.41 arbitrary units in control rats; p < 0.05). VEGF-induced pMF was attenuated by the MEK/ERK inhibitor U0126 [1,4-diamino-2,3-dicyano-1,4-bis(o-aminophenylmercapto)butadiene] and was abolished by the phosphotidinositol 3 kinase/Akt inhibitor LY294002 [2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride], demonstrating that ERK mitogen-activated protein kinases and Akt are both required for full expression of VEGF-induced pMF. This is the first report that VEGFA-165 elicits plasticity in any motor system. Furthermore, because VEGFA-165 expression is hypoxia sensitive, it may play a role in respiratory plasticity after prolonged exposures to low oxygen.


Subject(s)
Extracellular Signal-Regulated MAP Kinases/physiology , Motor Neurons/physiology , Phrenic Nerve/physiology , Proto-Oncogene Proteins c-akt/physiology , Signal Transduction/physiology , Spinal Cord/physiology , Vascular Endothelial Growth Factor A/physiology , Vascular Endothelial Growth Factor Receptor-2/physiology , Animals , Hypoxia/metabolism , Hypoxia/physiopathology , Male , Motor Neurons/enzymology , Phrenic Nerve/enzymology , Phrenic Nerve/metabolism , Rats , Rats, Sprague-Dawley , Spinal Cord/cytology , Spinal Cord/metabolism , Vascular Endothelial Growth Factor Receptor-2/biosynthesis
6.
Neuroscience ; 178: 45-55, 2011 Mar 31.
Article in English | MEDLINE | ID: mdl-21223996

ABSTRACT

Acute intermittent hypoxia (AIH) facilitates phrenic motor output by a mechanism that requires spinal serotonin (type 2) receptor activation, NADPH oxidase activity and formation of reactive oxygen species (ROS). Episodic spinal serotonin (5-HT) receptor activation alone, without changes in oxygenation, is sufficient to elicit NADPH oxidase-dependent phrenic motor facilitation (pMF). Here we investigated: (1) whether serotonin 2A and/or 2B (5-HT2A/B) receptors are expressed in identified phrenic motor neurons, and (2) which receptor subtype is capable of eliciting NADPH-oxidase-dependent pMF. In anesthetized, artificially ventilated adult rats, episodic C4 intrathecal injections (3×6 µl injections, 5 min intervals) of a 5-HT2A (DOI) or 5-HT2B (BW723C86) receptor agonist elicited progressive and sustained increases in integrated phrenic nerve burst amplitude (i.e. pMF), an effect lasting at least 90 min post-injection for both receptor subtypes. 5-HT2A and 5-HT2B receptor agonist-induced pMF were both blocked by selective antagonists (ketanserin and SB206553, respectively), but not by antagonists to the other receptor subtype. Single injections of either agonist failed to elicit pMF, demonstrating a need for episodic receptor activation. Phrenic motor neurons retrogradely labeled with cholera toxin B fragment expressed both 5-HT2A and 5-HT2B receptors. Pre-treatment with NADPH oxidase inhibitors (apocynin and diphenylenodium (DPI)) blocked 5-HT2B, but not 5-HT2A-induced pMF. Thus, multiple spinal type 2 serotonin receptors elicit pMF, but they act via distinct mechanisms that differ in their requirement for NADPH oxidase activity.


Subject(s)
Action Potentials/physiology , NADPH Oxidases/physiology , Phrenic Nerve/physiology , Receptor, Serotonin, 5-HT2A/physiology , Receptor, Serotonin, 5-HT2B/physiology , Acetophenones/administration & dosage , Acetophenones/pharmacology , Action Potentials/drug effects , Amphetamines/administration & dosage , Amphetamines/antagonists & inhibitors , Amphetamines/pharmacology , Animals , Indoles/administration & dosage , Indoles/antagonists & inhibitors , Indoles/pharmacology , Injections, Spinal , Ketanserin/administration & dosage , Ketanserin/pharmacology , Male , NADPH Oxidases/antagonists & inhibitors , Onium Compounds/pharmacology , Phrenic Nerve/drug effects , Phrenic Nerve/enzymology , Pyridines/pharmacology , Rats , Rats, Sprague-Dawley , Serotonin 5-HT2 Receptor Agonists/administration & dosage , Serotonin 5-HT2 Receptor Agonists/pharmacology , Serotonin 5-HT2 Receptor Antagonists/administration & dosage , Serotonin 5-HT2 Receptor Antagonists/pharmacology , Thiophenes/administration & dosage , Thiophenes/antagonists & inhibitors , Thiophenes/pharmacology
7.
Acta Histochem ; 113(7): 749-55, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21145575

ABSTRACT

Guanylyl cyclase (GC) as the effector molecule for nitric oxide (NO) plays a key role in the NO/cGMP signalling cascade. Based on these observations, our study focused on NO/sGC signalization in the bulbospinal respiratory pathway. The distribution of neuronal nitric oxide synthase (nNOS), ß1 subunit of soluble guanylyl cyclase (ß1sGC) and synaptophysin (SYN) was explored in the upper part of the respiratory pathway after C2-C3 hemisection of the spinal cord in male Wistar rats. Unilateral injection of Fluorogold into the phrenic nucleus (PN) at C4 level and survival of animals for 2 days revealed many Fluorogold fluorescent neurons in the ventral respiratory group (VRG) of the medulla, mostly on the contralateral side. Under physiological conditions we noted nNOS-fluorescent terminals of VRG neurons around ß1sGC fluorescent motoneurons in the PN. A strong depletion of nNOS/SYN fluorescent terminals was noted 8 days after hemisection around alpha motoneurons in the PN on the contralateral side. On the side of injury, nNOS/SYN fluorescent puncta were detected around phrenic motoneurons only sporadically. Phrenic alpha motoneurons responded to C2-C3 hemisection by a loss of ß1sGC positivity. The results confirm, that ß1sGC immunoreactive phrenic motoneurons are innervated by nNOS positive terminals coming from the VRG.


Subject(s)
Guanylate Cyclase/metabolism , Nitric Oxide/metabolism , Peripheral Nervous System Diseases/metabolism , Phrenic Nerve/metabolism , Signal Transduction , Spinal Cord Injuries/metabolism , Animals , Guanylate Cyclase/analysis , Male , Neural Pathways/enzymology , Neural Pathways/metabolism , Nitric Oxide/analysis , Peripheral Nervous System Diseases/enzymology , Phrenic Nerve/enzymology , Rats , Rats, Wistar , Spinal Cord Injuries/physiopathology , Spinal Cord Injuries/surgery
8.
Neuroscience ; 169(2): 787-93, 2010 Aug 25.
Article in English | MEDLINE | ID: mdl-20478365

ABSTRACT

Atypical protein kinase C (PKC) isoforms play important roles in many neural processes, including synaptic plasticity and neurodegenerative diseases. Although atypical PKCs are expressed throughout the brain, there are no reports concerning their expression in central neural regions associated with respiratory motor control. Therefore, we explored the neuroanatomical distribution of atypical PKCs in identified phrenic motor neurons, a motor pool that plays a key role in breathing. Diaphragm injections of cholera toxin B were used to retrogradely label and identify phrenic motor neurons; immunohistochemistry was used to localize atypical PKCs in and near labeled motor neurons (i.e. the phrenic motor nucleus). Atypical PKC expression in the phrenic motor nucleus appears specific to neurons; aPKC expression could not be detected in adjacent astrocytes or microglia. Strong atypical PKC labeling was observed within cholera toxin B labeled phrenic motor neurons. Documenting the expression of atypical PKCs in phrenic motor neurons provides a framework within which to assess their role in respiratory motor control, including novel forms of respiratory plasticity known to occur in this region.


Subject(s)
Motor Neurons/enzymology , Phrenic Nerve/enzymology , Protein Kinase C/biosynthesis , Animals , Isoenzymes/biosynthesis , Male , Neuroglia/enzymology , Phrenic Nerve/cytology , Rats , Rats, Inbred Lew
9.
J Neurosci ; 28(11): 2949-58, 2008 Mar 12.
Article in English | MEDLINE | ID: mdl-18337426

ABSTRACT

Phrenic long-term facilitation (pLTF) is a serotonin-dependent form of pattern-sensitive respiratory plasticity induced by intermittent hypoxia (IH), but not sustained hypoxia (SH). The mechanism(s) underlying pLTF pattern sensitivity are unknown. SH and IH may differentially regulate serine/threonine protein phosphatase activity, thereby inhibiting relevant protein phosphatases uniquely during IH and conferring pattern sensitivity to pLTF. We hypothesized that spinal protein phosphatase inhibition would relieve this braking action of protein phosphatases, thereby revealing pLTF after SH. Anesthetized rats received intrathecal (C4) okadaic acid (25 nm) before SH (25 min, 11% O(2)). Unlike (vehicle) control rats, SH induced a significant pLTF in okadaic acid-treated rats that was indistinguishable from rats exposed to IH (three 5 min episodes, 11% O(2)). IH and SH with okadaic acid may elicit pLTF by similar, serotonin-dependent mechanisms, because intravenous methysergide blocks pLTF in rats receiving IH or okadaic acid plus SH. Okadaic acid did not alter IH-induced pLTF. In summary, pattern sensitivity in pLTF may reflect differential regulation of okadaic acid-sensitive serine/threonine phosphatases; presumably, these phosphatases are less active during/after IH versus SH. The specific okadaic acid-sensitive phosphatase(s) constraining pLTF and their spatiotemporal dynamics during and/or after IH and SH remain to be determined.


Subject(s)
Hypoxia/enzymology , Long-Term Potentiation/physiology , Okadaic Acid/pharmacology , Phosphoprotein Phosphatases/physiology , Phrenic Nerve/enzymology , Animals , Hypoxia/physiopathology , Long-Term Potentiation/drug effects , Male , Phosphoprotein Phosphatases/analysis , Phrenic Nerve/chemistry , Phrenic Nerve/drug effects , Rats , Rats, Sprague-Dawley
10.
Exp Neurol ; 177(1): 115-32, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12429216

ABSTRACT

Previous investigations from our laboratory have documented that the neuropil of the phrenic nucleus contains a dense accumulation of punctate nicotinamide adenine dinucleotide phosphate diaphorase staining. In this study we investigated the occurrence and origin of punctate nitric oxide synthase immunoreactivity in the neuropil of the phrenic nucleus in C3-C5 segments, supposed to be the terminal field of the premotor bulbospinal respiratory nitric oxide synthase-immunoreactive pathway in the dog. As the first step, nitric oxide synthase immunohistochemistry was used to characterize nitric oxide synthase-immunoreactive staining of the phrenic nucleus and nitric oxide synthase-containing neurons in the dorsal and rostral ventral respiratory group and in the Bötzinger complex of the medulla. Dense punctate nitric oxide synthase immunoreactivity was found on control sections in the neuropil of the phrenic nucleus. Several thin bundles of nitric oxide synthase-immunoreactive fibers were found to enter the phrenic nucleus from the lateral and ventral column. Nitric oxide synthase-containing neurons were revealed in the dorsal respiratory group of medulla corresponding to the ventrolateral nucleus of the solitary tract and in the rostral ventral respiratory group beginning approximately 1 mm caudal to the obex and reaching to 650 microm rostral to the obex. Axotomy-induced retrograde changes, consisting in a strong upregulation of nitric oxide synthase-containing neurons, were found in the dorsal and rostral ventral respiratory group contralateral to the hemisection performed at the C2-C3 level. Concurrently, a strong depletion of the punctate nitric oxide synthase immunopositivity in the neuropil of the phrenic nucleus ipsilaterally with the hemisection was detected, thus revealing that a crossed premotor bulbospinal respiratory pathway contains a fairly high number of nitric oxide synthase-immunopositive fibers terminating in the phrenic nucleus. The use of the retrograde fluorescent tracer Fluorogold injected into the phrenic nucleus and an analysis of sections cut through the dorsal and rostral ventral respiratory group and Bötzinger complex of medulla and processed for nitric oxide synthase immunocytochemistry revealed that approximately 73.8% of crossed premotor bulbospinal respiratory nitric oxide synthase-immunoreactive axons originate in the rostral ventral respiratory group and 26.2% is given by nitric oxide synthase-containing neurons of the dorsal respiratory group. A few premotor nitric oxide synthase-immunoreactive axons originating from the Bötzinger complex were found. In summary, the present study provides evidence for a hitherto unknown premotor bulbospinal respiratory nitric oxide synthase-immunoreactive pathway connecting the bulbar respiratory centers with the motor neurons of the phrenic nucleus in the dog.


Subject(s)
Nitric Oxide Synthase/analysis , Phrenic Nerve/chemistry , Respiratory Center/chemistry , Animals , Dogs , Female , Immunohistochemistry , Male , Medulla Oblongata/chemistry , Medulla Oblongata/enzymology , Neural Pathways/chemistry , Neural Pathways/enzymology , Neurons/chemistry , Neurons/enzymology , Nitric Oxide Synthase/biosynthesis , Phrenic Nerve/enzymology , Respiratory Center/enzymology
11.
J Histochem Cytochem ; 46(11): 1261-8, 1998 Nov.
Article in English | MEDLINE | ID: mdl-9774625

ABSTRACT

Pre-embedding immunocytochemistry for the active form of glutamate decarboxylase (GAD67) and postembedding staining for gamma-aminobutyric acid (GABA) were compared as markers for central GABAergic terminals in the phrenic motor nucleus, in which phrenic motor neurons had been retrogradely labeled with cholera toxin B-horseradish peroxidase. Nerve terminals with or without GAD67 immunoreactivity were identified in one ultrathin section. GABA was localized with immunogold in an adjacent section after etching and bleaching. GABA labeling density was assessed over 519 GAD67-positive and GAD67-negative nerve terminals in the phrenic motor nucleus. Frequency histograms showed that statistically higher densities of gold particles occurred over most GAD67-positive terminals. However, some GAD67-negative terminals also showed high densities of gold particles, and some GAD67-positive terminals showed low densities. Preabsorption of the anti-GABA antibody with a GABA-protein conjugate, but not with other amino acid-protein conjugates, significantly reduced gold labeling over both GAD67-positive and GAD67-negative terminals. These results show that the presence of GAD67 immunoreactivity correlates strongly with high densities of immunogold labeling for GABA in nerve terminals in the phrenic motor nucleus. Preabsorption controls indicate that authentic GABA was localized in the postembedding labeling procedure. Only a small proportion of intensely GABA-immunoreactive terminals lack GAD67, suggesting that both GAD67 and GABA are reliable markers of GABAergic nerve terminals.


Subject(s)
Glutamate Decarboxylase/analysis , Nerve Endings/chemistry , Staining and Labeling/methods , gamma-Aminobutyric Acid/analysis , Animals , Cholera Toxin , Horseradish Peroxidase , Immunohistochemistry , Male , Microscopy, Electron , Nerve Endings/enzymology , Phrenic Nerve/chemistry , Phrenic Nerve/enzymology , Rats , Rats, Inbred WKY , Tissue Embedding
13.
Brain Res ; 441(1-2): 331-8, 1988 Feb 16.
Article in English | MEDLINE | ID: mdl-2451983

ABSTRACT

Caliber and microtubular density of myelinated fibers, acetylcholinesterase (AChE) content and its accumulation at a ligature were studied in the phrenic nerve of mature (3-4 months) and aging (2-year-old) rats. The number of axons remained constant. The cross-sectional area of the nerve was 67% greater in the older group; the axoplasm, though, constituted about 20% of the nerve tissue irrespective of age. The mean cross-sectional area of myelinated axons was twice as big in aging compared to mature rats. All axons grew in the same proportion irrespective of their original caliber. The microtubular density of 3-microns axons was about 22 microtubules/micron2 in mature and aging rats. The AChE activity of aging rats was half as much as that of mature rats if it was expressed per wet weight of nerve tissue but did not change if it was expressed per nerve fiber. Twenty-four hours after ligation of the nerve, total AChE activity rose in mature and aging rats by ca. 168%; the molecular forms--asymmetric and globular--accumulated in the same proportion in both age groups. We conclude that myelinated axons grow in the adult stage of life but the structure of axoplasm, content of AChE per axon, and rate of fast transport remain lifelong features of nerve fibers.


Subject(s)
Acetylcholinesterase/metabolism , Axonal Transport , Axons/physiology , Phrenic Nerve/growth & development , Aging , Animals , Axons/ultrastructure , Male , Microtubules/ultrastructure , Nerve Fibers, Myelinated/ultrastructure , Phrenic Nerve/enzymology , Phrenic Nerve/ultrastructure , Rats , Rats, Inbred Strains
14.
Brain Res ; 416(1): 69-74, 1987 Jul 21.
Article in English | MEDLINE | ID: mdl-3620957

ABSTRACT

The activities of choline acetyltransferase (CAT) and acetylcholinesterase (AChE) were assayed in intact diaphragm, extensor digitorum longus (EDL), and soleus muscles or their homogenates of young (2-6 months) and aged (24-34 months) mice. CAT activity (per mg of protein) was significantly higher in diaphragm and soleus of old mice in comparison with the young but the age change in EDL was negligible. On the other hand, AChE activity (per mg of protein) was significantly higher in EDL of old mice but in diaphragm and soleus muscles the enzyme activity did not show any significant change statistically. The diaphragm muscle was divided into two fractions, one being neuromuscular (NM) fraction and the other the remainder of the muscle (M fraction). No appreciable change in the ratio of the enzyme activities of NM fraction to the one of M fraction was obtained between the young and aged preparations. Thus, it seems likely that there is an age-related change in CAT and AChE activities which might be affected by the degree to which muscle activity is maintained.


Subject(s)
Acetylcholinesterase/metabolism , Aging/metabolism , Choline O-Acetyltransferase/metabolism , Muscles/enzymology , Neuromuscular Junction/enzymology , Animals , Diaphragm/enzymology , Diaphragm/physiology , Kinetics , Male , Mice , Mice, Inbred C57BL , Muscles/innervation , Organ Size , Phrenic Nerve/enzymology , Subcellular Fractions
15.
J Neurochem ; 48(5): 1502-8, 1987 May.
Article in English | MEDLINE | ID: mdl-3559564

ABSTRACT

Electrical stimulation of the phrenic nerve in an isolated nerve-diaphragm preparation resulted in the release of phosphatidylinositol phosphodiesterase into the organ bath. The released enzyme was Ca2+-dependent and exhibited two pH optima. The enzyme was released in response to nerve stimulation even in the presence of d-tubocurarine in concentrations that block neuromuscular transmission, and was not therefore released from the muscle as a consequence of its contractile activity. Phosphatidylinositol phosphodiesterase activity was determined in the soluble cytosol fractions prepared from different regions of skeletal muscles and from normal peripheral nerves and nerves that were degenerating after transection. The specific activity of the enzyme in the cytosol from the endplate-rich region of the diaphragm was significantly greater than that in cytosol from either the endplate-free region of the diaphragm or from the phrenic nerve. In degenerating nerve the activity of the enzyme was greater in the distal stump than in the proximal stump at 36 h after nerve section. Possible roles for released phosphatidylinositol phosphodiesterase at the neuromuscular junction are discussed.


Subject(s)
Diaphragm/enzymology , Phrenic Nerve/enzymology , Animals , Electric Stimulation , In Vitro Techniques , Nerve Endings/enzymology , Phrenic Nerve/physiology , Rats , Tubocurarine/pharmacology
16.
Proc R Soc Lond B Biol Sci ; 214(1195): 153-68, 1982 Jan 22.
Article in English | MEDLINE | ID: mdl-6127677

ABSTRACT

The compartmentation of acetylcholine (ACh) and of choline acetyltransferase in the rat diaphragm was analysed by measuring their contents in muscle segments containing endplates (e.p.) and endplate-free segments (non-e.p.) at different times following section of the phrenic nerve. In addition ACh release was determined before and after denervation. Freshly dissected hemidiaphragms contained about 125 pmol of ACh; more than 90% of this was localized in the e.p. portion. Between 10 and 18 h after denervation the ACh content of the e.p. portion decreased by 80% and its ACh concentration became approximately equal to that in the non-e.p. region, whose ACh content did not change. Spontaneous release of ACh was reduced by denervation and ACh release evoked by 50 mM KC1 was practically abolished. Choline acetyltransferase activity in freshly dissected preparations was about 30 nmol of ACh per gram per hour, Km 0.5 mM. About 65% of the enzyme disappeared in the first 24 h and the remaining 35% between 24 and 50 h after denervation. A different enzyme capable of ACh synthesis was found in the muscle fibres; its activity did not decrease after denervation. It is concluded that about 70% of the ACh in the diaphragm is contained in the motor nerve terminals, about 10% in the intramuscular nerve fibres and the remainder in the muscle fibres, and that about 65% of choline acetyltransferase is in the motor terminals and 35% in the nerve fibres.


Subject(s)
Acetylcholine/metabolism , Diaphragm/metabolism , Acetylcholine/biosynthesis , Animals , Choline O-Acetyltransferase/metabolism , Cholinesterases/metabolism , Diaphragm/innervation , Male , Motor Endplate/metabolism , Muscle Denervation , Phrenic Nerve/enzymology , Rats , Synaptic Transmission
18.
Anat Anz ; 146(3): 235-44, 1979.
Article in German | MEDLINE | ID: mdl-543560

ABSTRACT

As marked differences in the Acetylcholinesterase (ACHE)-activity of myelinated nerve fibres of ventral and dorsal spinal roots can be found also in human post mortem material (ZENKER et al. 1978), the Karnovsky-method for histochemical demonstration of ACHE-activity has been used for differentiation of motor and sensory fibres in the human phrenic nerve. In cross sections of the phrenic nerve 1--2 cm above its entrance into the diaphragm, after an incubation period of 24 hours, 86% of the medullated nerve fibres displayed a high enzyme activity and therefore were classified as motoric. The histogram of these stained fibres revealed a large group of fibres with a peak at 9--10 micron in diameter which were interpreted as A-alpha fibres and a small group of fibres with a peak at 2--3 micron which were classified as A-gamma fibres. The mean diameter of the A-alpha group fibres is smaller than the mean diameter in a "typical" muscle nerve. Furthermore, the number of A-gamma fibres in the phrenic nerve, as compared with a "typical" muscle nerve is strikingly small. This seems to be in accordance with the small number of unstained fibres (14% only) which were interpreted as sensoric. In this group no fibre was found larger than 9 micrometers in diameter. This could mean a complete lack of primary afferents within the human phrenic nerve.


Subject(s)
Acetylcholinesterase/metabolism , Nerve Fibers, Myelinated/anatomy & histology , Phrenic Nerve/anatomy & histology , Humans , Nerve Fibers, Myelinated/enzymology , Phrenic Nerve/enzymology
19.
Zh Mikrobiol Epidemiol Immunobiol ; (5): 111-6, 1975 May.
Article in Russian | MEDLINE | ID: mdl-1210900

ABSTRACT

On the appearance in the animals (guinea pigs) of paralysis of the limbs and myasthenia after the administration of Cl. botulinum, type B, toxin, there was seen a considerable vascular hyperemia of the spinal cord, and in the neurons of the phrenic nerve nucleus there developed dystrophic-necrotic processes coursing with a marked swelling, hyperchromasia and tigrolysis. As revealed histochemically, at this stage of the botulin intoxication the neurons of the phrenic nerve nucleus displayed metabolic changes expressed in the altered activity of succinic dehydrogenase, acid phosphatase and cholinesterase.


Subject(s)
Botulinum Toxins/pharmacology , Phrenic Nerve/drug effects , Acid Phosphatase/metabolism , Animals , Botulism/enzymology , Botulism/pathology , Cholinesterases/metabolism , Guinea Pigs , Motor Neurons/drug effects , Motor Neurons/enzymology , Phrenic Nerve/enzymology , Phrenic Nerve/pathology , Respiration/drug effects , Spinal Cord/pathology , Succinate Dehydrogenase/metabolism , Time Factors
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