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1.
Proc Natl Acad Sci U S A ; 121(23): e2407414121, 2024 Jun 04.
Article in English | MEDLINE | ID: mdl-38781224
2.
J Bodyw Mov Ther ; 38: 474-482, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38763596

ABSTRACT

OBJECTIVE: Restoring the degree of kyphosis to be consistent with good sagittal alignment of the spine is a key concern. This study aimed to compare the effect of core stability exercises (CSE) versus whole-body electromyostimulation (WB-EMS) and a combined program (PLUS) on kyphosis angle and core muscle endurance in sedentary individuals with hyperkyphosis. DESIGN: A quasi-experimental single group pre-post study. SETTINGS: Laboratory of corrective exercise. PARTICIPANTS: seventy-five untrained men (28.9 ± 5.3 years) with thoracic hyperkyphosis. MAIN OUTCOME MEASURES: A flexible ruler was used to measure the angle of kyphosis and McGill's test was used to evaluate core stability. RESULTS: The results of the post hoc test demonstrated that the kyphosis angle was improved in the WB-EMS and PLUS groups compared to that in the CG (P < 0.05), but no significant difference was observed among the three groups(P > 0.05). In the post-test, core stability was significantly improved in CSE, WB-EMS and PLUS groups compared to that in the CG. CONCLUSIONS: The WB-EMS and PLUS protocols as new training methods seem to be effective in changing posture parameters and correcting postural deformities, including kyphosis. Therefore, these protocols along with other rehabilitation programs can be used to correct kyphosis and improve core muscle endurance.


Subject(s)
Exercise Therapy , Kyphosis , Humans , Kyphosis/rehabilitation , Kyphosis/physiopathology , Male , Adult , Exercise Therapy/methods , Young Adult , Electric Stimulation Therapy/methods , Physical Endurance/physiology
3.
Sci Rep ; 14(1): 11676, 2024 05 22.
Article in English | MEDLINE | ID: mdl-38778135

ABSTRACT

Peak oxygen uptake (VO2), evaluated as exercise tolerance, is a strong predictor of life prognosis regardless of health condition. Several previous studies have reported that peak VO2 is higher in those with a greater decrease in muscle oxygen saturation (SmO2) in the active muscles during incremental exercise. However, the skeletal muscle characteristics of individuals exhibiting a greater decrease in SmO2 during active muscle engagement in incremental exercise remain unclear. This study aimed to clarify the relationship among muscle strength, muscle endurance, and skeletal muscle oxygenation dynamics in active leg muscles during incremental exercise. Twenty-four healthy young men were included and categorized into the non-moderate-to-high muscular strength and endurance group (those with low leg muscle strength, endurance, or both; n = 11) and the moderate-to-high muscular strength and endurance group (those with both moderate-to-high leg muscle strength and endurance; n = 13). All participants underwent cardiopulmonary exercise testing combined with near-infrared spectroscopy to assess whole-body peak VO2 and the change in SmO2 at the lateral vastus lateralis from rest to each exercise stage as skeletal muscle oxygenation dynamics. A linear mixed-effects model, with the change in SmO2 from rest to each stage as the dependent variable, individual participants as random effects, and group and exercise load as fixed effects, revealed significant main effects for both group (P = 0.001) and exercise load (P < 0.001) as well as a significant interaction between the two factors (P < 0.001). Furthermore, multiple-comparison test results showed that the change in SmO2 from rest to 40%-100% peak VO2 was significantly higher in the moderate-to-high muscular strength and endurance group than in the non-moderate-to-high muscular strength and endurance group. Maintaining both muscle strength and endurance at moderate or higher levels contributes to high skeletal muscle oxygenation dynamics (i.e., greater decrease in SmO2) during moderate- or high-intensity exercise.


Subject(s)
Muscle Strength , Muscle, Skeletal , Oxygen Consumption , Physical Endurance , Humans , Male , Muscle Strength/physiology , Muscle, Skeletal/metabolism , Muscle, Skeletal/physiology , Oxygen Consumption/physiology , Young Adult , Physical Endurance/physiology , Adult , Exercise/physiology , Exercise Test , Spectroscopy, Near-Infrared , Oxygen/metabolism , Oxygen Saturation/physiology
4.
Nature ; 629(8010): 174-183, 2024 May.
Article in English | MEDLINE | ID: mdl-38693412

ABSTRACT

Regular exercise promotes whole-body health and prevents disease, but the underlying molecular mechanisms are incompletely understood1-3. Here, the Molecular Transducers of Physical Activity Consortium4 profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome and immunome in whole blood, plasma and 18 solid tissues in male and female Rattus norvegicus over eight weeks of endurance exercise training. The resulting data compendium encompasses 9,466 assays across 19 tissues, 25 molecular platforms and 4 training time points. Thousands of shared and tissue-specific molecular alterations were identified, with sex differences found in multiple tissues. Temporal multi-omic and multi-tissue analyses revealed expansive biological insights into the adaptive responses to endurance training, including widespread regulation of immune, metabolic, stress response and mitochondrial pathways. Many changes were relevant to human health, including non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular health and tissue injury and recovery. The data and analyses presented in this study will serve as valuable resources for understanding and exploring the multi-tissue molecular effects of endurance training and are provided in a public repository ( https://motrpac-data.org/ ).


Subject(s)
Endurance Training , Multiomics , Physical Conditioning, Animal , Physical Endurance , Animals , Female , Humans , Male , Rats , Acetylation , Blood/immunology , Blood/metabolism , Cardiovascular Diseases/genetics , Cardiovascular Diseases/immunology , Cardiovascular Diseases/metabolism , Databases, Factual , Epigenome , Inflammatory Bowel Diseases/genetics , Inflammatory Bowel Diseases/immunology , Inflammatory Bowel Diseases/metabolism , Internet , Lipidomics , Metabolome , Mitochondria/metabolism , Non-alcoholic Fatty Liver Disease/genetics , Non-alcoholic Fatty Liver Disease/immunology , Non-alcoholic Fatty Liver Disease/metabolism , Organ Specificity/genetics , Organ Specificity/immunology , Organ Specificity/physiology , Phosphorylation , Physical Conditioning, Animal/physiology , Physical Endurance/genetics , Physical Endurance/physiology , Proteome/metabolism , Proteomics , Time Factors , Transcriptome/genetics , Ubiquitination , Wounds and Injuries/genetics , Wounds and Injuries/immunology , Wounds and Injuries/metabolism
5.
Nutrients ; 16(9)2024 Apr 26.
Article in English | MEDLINE | ID: mdl-38732553

ABSTRACT

Considering a lack of respective data, the primary objective of this study was to assess whether seasonal variation in vitamin D status (D-status) affects the extent of improvement in physical performance (PP) in conscripts during basic military training (BMT). D-status, PP and several blood parameters were measured repeatedly in conscripts whose 10-week BMT started in July (cohort S-C; n = 96) or in October (cohort A-C; n = 107). D-status during BMT was higher in S-C compared to A-C (overall serum 25(OH)D 61.4 ± 16.1 and 48.5 ± 20.7 nmol/L, respectively; p < 0.0001). Significant (p < 0.05) improvements in both aerobic and muscular endurance occurred in both cohorts during BMT. Pooled data of the two cohorts revealed a highly reliable (p = 0.000) but weak (R2 = 0.038-0.162) positive association between D-status and PP measures both at the beginning and end of BMT. However, further analysis showed that such a relationship occurred only in conscripts with insufficient or deficient D-status, but not in their vitamin D-sufficient companions. Significant (p < 0.05) increases in serum testosterone-to-cortisol ratio and decreases in ferritin levels occurred during BMT. In conclusion, a positive association exists between D-status and PP measures, but seasonal variation in D-status does not influence the extent of improvement in PP in conscripts during BMT.


Subject(s)
Military Personnel , Physical Endurance , Seasons , Vitamin D , Humans , Vitamin D/blood , Vitamin D/analogs & derivatives , Male , Physical Endurance/physiology , Young Adult , Hydrocortisone/blood , Vitamin D Deficiency/blood , Vitamin D Deficiency/epidemiology , Nutritional Status , Testosterone/blood , Adult , Cohort Studies , Adolescent
6.
Nutrients ; 16(9)2024 Apr 30.
Article in English | MEDLINE | ID: mdl-38732600

ABSTRACT

BACKGROUND: Exercise and the consumption of sugars result in a dysfunction of the intestinal barrier (IB). Here, we determined the effect of sugar in a natural matrix on the intestinal barrier after moderate (A) and intensive endurance exercise (B). METHOD: The IB function was determined before (pre) and after running (post), and 120 and 180 min after consuming the drink by measuring serum endotoxin concentrations (lipopolysaccharides-LPS), IL-6, CD14, and i-FABP. In study A, nonspecifically trained participants (n = 24, males and females, age 26 ± 4) ran for one hour at 80% of their individual anaerobic threshold (IAT). After finishing, the runners consumed, in a crossover setup, either 500 mL of water, diluted cloudy apple juice (test drink), or an identical drink (placebo) without the fruit juice matrix (FJM). In study B, the participants (n = 30, males and females, age 50 ± 9) completed an ultra-marathon run, were divided into groups, and consumed one of the above-mentioned drinks. RESULTS: Study A: Exercise resulted in a significant increase in serum LPS, i-FABP, and IL-6, which decreased fast after finishing. No impact of the different drinks on LPS i-FABP, or IL-6 could be observed, but there was an impact on CD14. Study B: The ultra-marathon resulted in a strong increase in serum LPS, which decreased fast after finishing in the water and test drink groups, but not in the placebo group. CONCLUSIONS: The consumed drinks did not affect the kinetics of IB regeneration after moderate exercise, but impacted CD14 serum concentrations, indicating possible beneficial effects of the FJM on the immune system. After an ultra-marathon, IB function regenerates very fast. The intake of sugar (placebo) seems to have had a negative impact on IB regeneration, which was diminished by the presence of the FJM.


Subject(s)
Cross-Over Studies , Fruit and Vegetable Juices , Interleukin-6 , Lipopolysaccharide Receptors , Malus , Marathon Running , Physical Endurance , Polyphenols , Humans , Male , Female , Adult , Middle Aged , Polyphenols/pharmacology , Polyphenols/administration & dosage , Physical Endurance/drug effects , Physical Endurance/physiology , Interleukin-6/blood , Lipopolysaccharide Receptors/blood , Marathon Running/physiology , Intestinal Mucosa/metabolism , Intestinal Mucosa/drug effects , Lipopolysaccharides/blood , Fatty Acid-Binding Proteins/blood , Running/physiology , Young Adult
7.
Sci Rep ; 14(1): 11321, 2024 05 17.
Article in English | MEDLINE | ID: mdl-38760471

ABSTRACT

This study aimed to analyze anthropometric and physiological profiles of highly trained sailors and the differences between sailors regarding various training levels. Forty-two sailors (22 male, 22.4 ± 3.8 years; 20 females, 21.3 ± 3.6 years) were divided into helmsmen and crew groups, and the high- and low-level were distinguished. Sailors completed height, sitting height, legs length, weight, BMI, VO2max, 30 s all-out sprint, isometric mid-thigh pull (IMTP), countermovement jump, bench pull, core endurance tests. The results showed the crew had higher height, sitting height, weight, VO2max and lower trunk flexor endurance test times compared to the helmsmen (p < 0.05). The helmsmen had higher relative peak power/force in the 30 s all-out sprint and IMTP tests compared to the crew, whereas the crew had better absolute strength in bench pull, with significant differences between female sailors (p < 0.05). The high-level sailors showed more sailing experience than low-level sailors (p < 0.05). In conclusion, highly trained crew tend to be taller and heavier, while helmsmen have better trunk flexor endurance. For female sailors, helmsmen have better lower-body power and strength and crew have better upper-body strength. Sailing experience is a reliable variable to distinguish sailors' levels. The specific anthropometric and physiological profiles of sailors in various positions can assist sailing coaches in athlete selection and intervention training.


Subject(s)
Anthropometry , Physical Endurance , Humans , Female , Male , Adult , Physical Endurance/physiology , Young Adult , Water Sports/physiology , Muscle Strength/physiology , Ships , Oxygen Consumption/physiology , Military Personnel , Body Weight
8.
Nutrients ; 16(10)2024 May 09.
Article in English | MEDLINE | ID: mdl-38794667

ABSTRACT

BACKGROUND: Various nutritional strategies are increasingly used in sports to reduce oxidative stress and promote recovery. Chokeberry is rich in polyphenols and can reduce oxidative stress. Consequently, chokeberry juices and mixed juices with chokeberry content are increasingly used in sports. However, the data are very limited. Therefore, this study investigates the effects of the short-term supplementation of a red fruit juice drink with chokeberry content or a placebo on muscle damage, oxidative status, and leg strength during a six-day intense endurance protocol. METHODS: Eighteen recreational endurance athletes participated in a cross-over high intensity interval training (HIIT) design, receiving either juice or a placebo. Baseline and post-exercise assessments included blood samples, anthropometric data, and leg strength measurements. RESULTS: A significant increase was measured in muscle damage following the endurance protocol in all participants (∆ CK juice: 117.12 ± 191.75 U/L, ∆ CK placebo: 164.35 ± 267.00 U/L; p = 0.001, η2 = 0.17). No group effects were detected in exercise-induced muscle damage (p = 0.371, η2 = 0.010) and oxidative status (p = 0.632, η2 = 0.000). The reduction in strength was stronger in the placebo group, but group effects are missing statistical significance (∆ e1RM juice: 1.34 ± 9.26 kg, ∆ e1RM placebo: -3.33 ± 11.49 kg; p = 0.988, η2 = 0.000). CONCLUSION: Although a reduction in strength can be interpreted for the placebo treatment, no statistically significant influence of chokeberry could be determined. It appears that potential effects may only occur with prolonged application and a higher content of polyphenols, but further research is needed to confirm this.


Subject(s)
Athletes , Cross-Over Studies , Fruit and Vegetable Juices , Muscle Strength , Physical Endurance , Polyphenols , Humans , Polyphenols/pharmacology , Male , Adult , Muscle Strength/drug effects , Physical Endurance/drug effects , Physical Endurance/physiology , Young Adult , Female , Oxidative Stress/drug effects , Leg/physiology , Double-Blind Method , Fruit/chemistry , Photinia/chemistry , Muscle, Skeletal/drug effects , Muscle, Skeletal/physiology , Muscle, Skeletal/metabolism , Exercise/physiology , Endurance Training/methods
9.
Narra J ; 4(1): e303, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38798840

ABSTRACT

Trunk muscles maintain steady effort with adequate strength and endurance. When the muscle performance is subpar, it might cause lower back discomfort. No reference for trunk strength and endurance has been established previously. The aim of this study was to determine the normative reference values for dynamometric and non-dynamometric tests in people with various body fat percentages. Two hundred sixty-four participants aged 19-40 years old were recruited in this cross-sectional study. The Siri equation was used to calculate the individuals body fat proportions, which were divided into normal, high, and very high body fat for men and women. The Modified Sorenson's and the Back-Leg-Chest Dynamometric tests were utilized to measure muscular performance. The means of strength in females with normal, high, and very high body fat percentages were 27.39, 25.75, and 25.37 N/m2, respectively. The males in the same category had the means of 56.48, 51.79, and 60.17 N/m2, respectively. The highest mean of endurance in females was in those with normal body fat percentage (42.28), so did males (71.02). Our findings suggest that males had higher trunk muscle strength and endurance than females, and normal-body-fat individuals had the greatest endurance regardless of gender.


Subject(s)
Muscle Strength , Humans , Female , Male , Adult , Cross-Sectional Studies , Muscle Strength/physiology , Reference Values , Sex Factors , Adipose Tissue , Muscle Strength Dynamometer , Torso/physiology , Physical Endurance/physiology , Young Adult
10.
Int J Mol Sci ; 25(9)2024 Apr 23.
Article in English | MEDLINE | ID: mdl-38731803

ABSTRACT

This study explores the effects of normobaric hypoxia and intermittent hypoxic training (IHT) on the physiological condition of the cardiac muscle in swimmers. Hypoxia has been reported to elicit both beneficial and adverse changes in the cardiovascular system, but its impact on the myocardium during acute exercise and altitude/hypoxic training remains less understood. We aimed to determine how a single bout of intense interval exercise and a four-week period of high-intensity endurance training under normobaric hypoxia affect cardiac marker activity in swimmers. Sixteen young male swimmers were divided into two groups: one undergoing training in hypoxia and the other in normoxia. Cardiac markers, including troponin I and T (cTnI and cTnT), heart-type fatty acid-binding protein (H-FABP), creatine kinase-MB isoenzyme (CK-MB), and myoglobin (Mb), were analyzed to assess the myocardium's response. We found no significant differences in the physiological response of the cardiac muscle to intense physical exertion between hypoxia and normoxia. Four weeks of IHT did not alter the resting levels of cTnT, cTnI, and H-FABP, but it resulted in a noteworthy decrease in the resting concentration of CK-MB, suggesting enhanced cardiac muscle adaptation to exercise. In contrast, a reduction in resting Mb levels was observed in the control group training in normoxia. These findings suggest that IHT at moderate altitudes does not adversely affect cardiac muscle condition and may support cardiac muscle adaptation, affirming the safety and efficacy of IHT as a training method for athletes.


Subject(s)
Athletes , Biomarkers , Hypoxia , Humans , Male , Hypoxia/metabolism , Pilot Projects , Swimming/physiology , Young Adult , Myocardium/metabolism , Myoglobin/metabolism , Troponin I/metabolism , Fatty Acid Binding Protein 3/metabolism , Adolescent , Fatty Acid-Binding Proteins/metabolism , Physical Endurance/physiology , Creatine Kinase, MB Form/blood , Creatine Kinase, MB Form/metabolism , Adaptation, Physiological , Altitude
11.
Physiol Rep ; 12(10): e16058, 2024 May.
Article in English | MEDLINE | ID: mdl-38769072

ABSTRACT

We developed a test to evaluate badminton-specific endurance. The study included 10 female badminton players. Five participants were ranked in Japan's top 100 national rankings (ranked), whereas the others were unranked (unranked). Participants reacted quickly with badminton-specific steps from the base center to the four sensors at each corner of a singles badminton court. On each set, they reacted eight times to randomized instructions at stage-specific intervals (1.2, 1.0, and 0.8 s for stages 1, 2, and 3, respectively), which were performed six times with a rest of 20 s in each stage (8 movements × 6 sets × 3 stages). On a different day, participants ran on a treadmill as a comparative test. Blood lactate concentration (BLa) was measured on each test. In the badminton-specific test, ranked participants had lower BLa (4.2 ± 1.7 mM vs. 6.3 ± 3.1 mM), with medium or large effect sizes. The average reach time to sensors was shorter in ranked participants (1.56 ± 0.03 s vs. 1.62 ± 0.07 s), with medium or large effect sizes. BLa was similar between groups, with trivial or small effect sizes in the running test. These results suggest that the newly developed test can evaluate badminton-specific endurance.


Subject(s)
Exercise Test , Physical Endurance , Racquet Sports , Humans , Female , Physical Endurance/physiology , Pilot Projects , Adult , Exercise Test/methods , Exercise Test/standards , Lactic Acid/blood , Young Adult
12.
Sci Rep ; 14(1): 11500, 2024 05 20.
Article in English | MEDLINE | ID: mdl-38769417

ABSTRACT

The dissociation of effects of age, time of assessment and cohort is a well-known challenge in developmental science. We examined effects of time of assessment in the school year on children's physical fitness using data from 75,362 German third-graders from seven cohorts. Children were tested once either in the first or second school term of third grade. Tests examined cardiorespiratory endurance (6-min run), coordination (star-run), speed (20-m sprint), lower (standing long jump) and upper (ball-push test) limbs muscle power, and flexibility (stand-and-reach test). We estimated the effect of time of assessment using a regression discontinuity design specified in a linear mixed model with random factors child and school and adjusted for age, sex, and cohort effects. Coordination, speed, and upper limbs muscle power were better in second compared to first school term, with boys exhibiting a larger increase of upper limbs muscle power than girls. There was no evidence for changes in cardiorespiratory endurance, lower limbs muscle power, and flexibility between assessments. Previously reported age and sex effects as well as secular fitness trends were replicated. There is thus evidence for improvement of some physical fitness components beyond age and cohort effects that presumably reflects the benefit of physical activity in physical education and other settings. Effects of assessment time should be taken into consideration in performance-based grading or norm-based selection of children.


Subject(s)
Physical Fitness , Schools , Humans , Male , Female , Child , Physical Fitness/physiology , Cardiorespiratory Fitness/physiology , Exercise/physiology , Germany , Muscle Strength/physiology , Time Factors , Physical Endurance/physiology
13.
Lasers Med Sci ; 39(1): 132, 2024 May 17.
Article in English | MEDLINE | ID: mdl-38758297

ABSTRACT

Photobiomodulation therapy (PBMT) was introduced as an ergogenic aid for sport performance in healthy individuals is still controversial. The main aim of this study is to assess the potential enhancements in muscle endurance and recovery from muscle strength and injuries mediated by PBMT among individuals exhibiting diverse activity levels. Randomized controlled trials (RCT) of PBMT interventions for healthy people (both trained and untrained individuals) exercising were searched (up to January 16, 2024) in four electronic databases: Web of Science, PubMed, Scopus and Embase. Primary outcome measures included muscle endurance, muscle strength and creatine kinase (CK) levels; secondary outcome measure included Lactate dehydrogenase (LDH) levels. Subgroup analyses based on physical activity levels were conducted for each outcome measure. Thirty-four RCTs were included based on the article inclusion and exclusion criteria. Statistical results showed that PBMT significantly improved muscle endurance (standardized mean difference [SMD] = 0.31, 95%CI 0.11, 0.51, p < 0.01), indicating a moderate effect size. It also facilitated the recovery of muscle strength (SMD = 0.24, 95%CI 0.10, 0.39, p < 0.01) and CK (mean difference [MD] = -77.56, 95%CI -112.67, -42.44, p < 0.01), indicating moderate and large effect sizes, respectively. Furthermore, pre-application of PBMT significantly improved muscle endurance, recovery of muscle strength and injuries in physically inactive individuals and athletes (p < 0.05), while there was no significant benefit for physically active individuals. Pre-application of PBMT improves muscle endurance and promotes recovery from muscle strength and injury (includes CK and LDH) in athletes and sedentary populations, indicating moderate to large effect sizes, but is ineffective in physically active populations. This may be due to the fact that physically active people engage in more resistance training, which leads to a decrease in the proportion of red muscle fibres, thus affecting photobiomodulation.


Subject(s)
Low-Level Light Therapy , Muscle Strength , Physical Endurance , Randomized Controlled Trials as Topic , Humans , Low-Level Light Therapy/methods , Muscle Strength/radiation effects , Muscle Strength/physiology , Physical Endurance/radiation effects , Physical Endurance/physiology , Exercise/physiology , Creatine Kinase/blood , Muscle, Skeletal/radiation effects , Muscle, Skeletal/physiology
14.
An Acad Bras Cienc ; 96(2): e20230559, 2024.
Article in English | MEDLINE | ID: mdl-38747788

ABSTRACT

Creatine is consumed by athletes to increase strength and gain muscle. The aim of this study was to evaluate the effects of creatine supplementation on maximal strength and strength endurance. Twelve strength-trained men (25.2 ± 3.4 years) supplemented with 20 g Creatina + 10g maltodextrin or placebo (20g starch + 10g maltodextrin) for five days in randomized order. Maximal strength and strength endurance (4 sets 70% 1RM until concentric failure) were determined in the bench press. In addition, blood lactate, rate of perceived effort, fatigue index, and mood state were evaluated. All measurements were performed before and after the supplementation period. There were no significant changing in maximal strength, blood lactate, RPE, fatigue index, and mood state in either treatment. However, the creatine group performed more repetitions after the supplementation (Cr: Δ = +3.4 reps, p = 0.036, g = 0.53; PLA: Δ = +0.3reps, p = 0.414, g = 0.06), and higher total work (Cr: Δ = +199.5au, p = 0.038, g = 0.52; PLA: Δ = +26.7au, p = 0.402, g = 0.07). Creatine loading for five days allowed the subjects to perform more repetitions, resulting in greater total work, but failed to change the maximum strength.


Subject(s)
Creatine , Dietary Supplements , Lactic Acid , Muscle Strength , Physical Endurance , Humans , Male , Adult , Creatine/administration & dosage , Creatine/pharmacology , Creatine/blood , Muscle Strength/drug effects , Muscle Strength/physiology , Physical Endurance/drug effects , Physical Endurance/physiology , Lactic Acid/blood , Young Adult , Resistance Training/methods , Muscle Fatigue/drug effects , Muscle Fatigue/physiology , Double-Blind Method
15.
J Int Soc Sports Nutr ; 21(1): 2341903, 2024 Dec.
Article in English | MEDLINE | ID: mdl-38626029

ABSTRACT

Protein supplementation often refers to increasing the intake of this particular macronutrient through dietary supplements in the form of powders, ready-to-drink shakes, and bars. The primary purpose of protein supplementation is to augment dietary protein intake, aiding individuals in meeting their protein requirements, especially when it may be challenging to do so through regular food (i.e. chicken, beef, fish, pork, etc.) sources alone. A large body of evidence shows that protein has an important role in exercising and sedentary individuals. A PubMed search of "protein and exercise performance" reveals thousands of publications. Despite the considerable volume of evidence, it is somewhat surprising that several persistent questions and misconceptions about protein exist. The following are addressed: 1) Is protein harmful to your kidneys? 2) Does consuming "excess" protein increase fat mass? 3) Can dietary protein have a harmful effect on bone health? 4) Can vegans and vegetarians consume enough protein to support training adaptations? 5) Is cheese or peanut butter a good protein source? 6) Does consuming meat (i.e., animal protein) cause unfavorable health outcomes? 7) Do you need protein if you are not physically active? 8) Do you need to consume protein ≤ 1 hour following resistance training sessions to create an anabolic environment in skeletal muscle? 9) Do endurance athletes need additional protein? 10) Does one need protein supplements to meet the daily requirements of exercise-trained individuals? 11) Is there a limit to how much protein one can consume in a single meal? To address these questions, we have conducted a thorough scientific assessment of the literature concerning protein supplementation.


Subject(s)
Dietary Proteins , Physical Endurance , Humans , Physical Endurance/physiology , Exercise/physiology , Dietary Supplements , Muscle, Skeletal/physiology
17.
J Appl Physiol (1985) ; 136(5): 1284-1290, 2024 May 01.
Article in English | MEDLINE | ID: mdl-38572538

ABSTRACT

Despite the prognostic effect of physical activity, acute bouts of high-volume endurance exercise can induce cardiac stress and postexercise hypercoagulation associated with increased thrombotic risk. The aim of this study was to explore the effect of high-volume endurance exercise on coagulation and thrombotic activity in recreational cyclists. Thirty-four recreational cyclists completed 4.8 ± 0.3 h of cycling at 45 ± 5% of maximal power output on a bicycle ergometer. Intravenous blood samples were collected preexercise, immediately postexercise, 24 and 48 h postexercise, and analyzed for brain natriuretic peptide (BNP), cardiac troponin (cTn), C-reactive protein (CRP), D-dimer, thrombin-antithrombin (TAT) complex, tissue factor (TF), tissue factor pathway inhibitor (TFPI), and TF-to-TFPI ratio (TF:TFPI). An increase in cTn was observed postexercise (P < 0.001). CRP concentrations were increased at 24 and 48 h postexercise compared with preexercise concentrations (P ≤ 0.001). TF was elevated at 24 h postexercise (P < 0.031) and TFPI was higher immediately postexercise (P < 0.044) compared with all other time points. TF:TFPI was increased at 24 and 48 h postexercise compared with preexercise (P < 0.025). TAT complex was reduced at 48 h postexercise compared with preexercise (P = 0.015), D-dimer was higher immediately postexercise compared with all other time points (P ≤ 0.013). No significant differences were observed in BNP (P > 0.05). High-volume endurance cycling induced markers of cardiac stress among recreational cyclists. However, plasma coagulation and fibrinolytic activity suggest no increase in thrombotic risk after high-volume endurance exercise.NEW & NOTEWORTHY In this study, a high-volume endurance exercise protocol induced markers of cardiac stress and altered plasma coagulation and fibrinolytic activity for up to 48 h in recreationally active cyclists. However, analysis of coagulation biomarkers indicates no increase in thrombotic risk when appropriate hydration and rest protocols are implemented.


Subject(s)
Bicycling , Blood Coagulation , Physical Endurance , Thromboplastin , Thrombosis , Humans , Bicycling/physiology , Male , Blood Coagulation/physiology , Adult , Thrombosis/physiopathology , Thrombosis/blood , Thrombosis/etiology , Physical Endurance/physiology , Thromboplastin/metabolism , C-Reactive Protein/metabolism , Fibrin Fibrinogen Degradation Products/metabolism , Exercise/physiology , Natriuretic Peptide, Brain/blood , Young Adult , Lipoproteins/blood , Biomarkers/blood , Antithrombin III/metabolism , Risk Factors , Peptide Hydrolases/blood
18.
J Am Heart Assoc ; 13(9): e033317, 2024 May 07.
Article in English | MEDLINE | ID: mdl-38686869

ABSTRACT

BACKGROUND: Although moderate endurance exercise has been reported to improve cardiovascular health, its effects on cardiac structure and function are not fully characterized, especially with respect to sexual dimorphism. We aimed to assess the effects of moderate endurance exercise on cardiac physiology in male versus female mice. METHODS AND RESULTS: C57BL/6J mice of both sexes were run on a treadmill for 6 weeks. ECG and echocardiography were performed every 2 weeks. After 6 weeks of exercise, mice were euthanized, and triple parametric optical mapping was performed on Langendorff perfused hearts to assess cardiac electrophysiology. Arrhythmia inducibility was tested by programmed electrical stimulation. Left ventricular tissue was fixed, and RNA sequencing was performed to determine exercise-induced transcriptional changes. Exercise-induced left ventricular dilatation was observed in female mice alone, as evidenced by increased left ventricular diameter and reduced left ventricular wall thickness. Increased cardiac output was also observed in female exercised mice but not males. Optical mapping revealed further sexual dimorphism in exercise-induced modulation of cardiac electrophysiology. In female mice, exercise prolonged action potential duration and reduced voltage-calcium influx delay. In male mice, exercise reduced the calcium decay constant, suggesting faster calcium reuptake. Exercise increased arrhythmia inducibility in both male and female mice; however, arrhythmia duration was increased only in females. Lastly, exercise-induced transcriptional changes were sex dependent: females and males exhibited the most significant changes in contractile versus metabolism-related genes, respectively. CONCLUSIONS: Our data suggest that moderate endurance exercise can significantly alter multiple aspects of cardiac physiology in a sex-dependent manner. Although some of these effects are beneficial, like improved cardiac mechanical function, others are potentially proarrhythmic.


Subject(s)
Arrhythmias, Cardiac , Mice, Inbred C57BL , Physical Conditioning, Animal , Animals , Female , Male , Arrhythmias, Cardiac/physiopathology , Arrhythmias, Cardiac/etiology , Physical Conditioning, Animal/physiology , Mice , Sex Factors , Ventricular Function, Left/physiology , Action Potentials , Physical Endurance/physiology , Ventricular Remodeling/physiology , Heart Rate/physiology , Isolated Heart Preparation , Sex Characteristics
19.
Scand J Med Sci Sports ; 34(5): e14637, 2024 May.
Article in English | MEDLINE | ID: mdl-38671555

ABSTRACT

During prolonged running at moderate-to-high intensity, running economy (RE) deteriorates and attainable maximal oxygen consumption (VO2max) decreases. Whether these changes appear similarly in trained and untrained runners exercising at the same relative intensity is not clear. We recruited 10 trained runners (TR) and 10 active adults (AA), and compared RE and attainable VO2max before and after 1 h of running at 70% of VO2max. Submaximal VO2 increased more (p = 0.019) in AA (0.20 ± 0.13 L min-1) than in TR (0.07 ± 0.05 L min-1). Attainable VO2max decreased in AA (-0.21 ± 0.15 L min-1, p = 0.002), but remained unchanged in TR (-0.05 ± 0.10 L min-1, p = 0.18). Relative intensity (i.e., VO2/attainable VO2max), increased more (p = 0.001) in AA (8.3 ± 4.4%) than in TR (2.6 ± 1.9%). These results demonstrate that the ability to resist changes in RE and VO2max following prolonged running is superior in trained versus untrained runners, when exercising at the same relative intensity.


Subject(s)
Oxygen Consumption , Running , Humans , Oxygen Consumption/physiology , Running/physiology , Male , Adult , Young Adult , Female , Physical Endurance/physiology , Physical Conditioning, Human/physiology , Physical Conditioning, Human/methods
20.
Braz J Cardiovasc Surg ; 39(2): e20230231, 2024 Apr 03.
Article in English | MEDLINE | ID: mdl-38568942

ABSTRACT

INTRODUCTION: Protocols for obtaíníng the maxímum threshold pressure have been applied wíth límited precision to evaluate ínspiratory muscle endurance. In thís sense, new protocols are needed to allow more relíable measurements. The purpose of the present study was to compare a new incremental ramp load protocol for the evaluation of ínspíratory muscle endurance wíth the most used protocol in healthy indíviduals. METHODS: This was a prospective cross-sectional study carried out ín a síngle center. Nínety-two healthy indíviduals (43 men [22 ± 3 years] and 49 women [22 ± 3 years]) were randomly allocated to perform: (i) íncremental ramp load protocol and (íí) íncremental step loadíng protocol. The sustained pressure threshold (or maximum threshold pressure), maximum threshold pressure/dynamic strength índex ratío, time untíl task faílure, as well as dífference between the mean heart rate of the last five mínutes of baselíne and the peak heart rate of the last 30 seconds of each protocol were measured. RESULTS: Incremental ramp load protocol wíth small íncreases in the load and starting from mínímum values of strength index was able to evaluate the inspiratory muscle endurance through the maxímum threshold pressure of healthy indívíduals. CONCLUSION: The present study suggests that the íncremental ramp load protocol is able to measure maximum threshold pressure in a more thorough way, wíth less progression and greater accuracy in the load stratification compared to the límited incremental step loading protocol and with a safe and expected cardiovascular response in healthy individuals.


Subject(s)
Exercise Test , Physical Endurance , Male , Humans , Female , Physical Endurance/physiology , Cross-Sectional Studies , Prospective Studies , Respiratory Muscles/physiology , Randomized Controlled Trials as Topic
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