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1.
Bol. latinoam. Caribe plantas med. aromát ; 23(4): 523-533, jul. 2024. tab
Article in English | LILACS | ID: biblio-1538056

ABSTRACT

Leaves of Croton stipulaceuswere extracted (EHex, ECHCl3and EEtOH extracts) to assesstheir antioxidant potential, anti-inflammatory activity in murine models and acute toxicity. EEtOH showed the highest effect in DPPH (37.80% inhibition), FRAP (1065.00 ± 55.30 µmolFe2+) and total polyphenols (231.24 ± 9.05 meq AG/gM). EHex was the most active, ~ 50% inhibition of TPA-induced ear edema; while EEtOH (dose of 2 mg/ear) showed the highest inhibition in the chronic model (97% inhibition), and inhibited MPO activity (48%). In carrageenan-induced edema, ECHCl3(dose 500 mg/kg) was the most active. None of the extracts showed acute toxicity (LD50) at 2 g/kg (p.o.). This work is the first report that supports the traditional use of C. stipulaceusas an anti-inflammatory.


De las hojas de Croton stipulaceusse obtuvieron diferentes extractos (EHex, ECHCl3y EEtOH) evaluando el potencial antioxidante y la actividad antiinflamatoria en modelos murinos y la toxicidad aguda. El EEtOH mostró mayor efecto en DPPH (37.80% inhibición), FRAP (1065.00 ± 55.30 µmolFe2+) y polifenolestotales (231.24 ± 9.05 meq AG/gM). El EHex fue el más activo, cercano al 50% de inhibición del edema auricular inducido con TPA; mientras que el EEtOH (dosis de 2 mg/oreja) mostró la mayor inhibición en el modelo crónico (97% inhibición), e inhibió la actividad de la MPO (48%). En el edema inducido con carragenina, el ECHCl3(dosis 500 mg/kg) fue el más activo. Ninguno de los extractos mostró una toxicidad aguda (DL50) mayor a 2 g/kg (p.o). Este trabajo es el primer reporte que sustenta el uso tradicional de C. stipulaceuscomo antiinflamatorio.


Subject(s)
Plant Leaves/chemistry , Croton/chemistry , Plant Extracts/metabolism , Plant Extracts/chemistry , Plant Structures/metabolism , Plant Structures/chemistry , Plant Leaves/metabolism , Croton/metabolism , Anti-Inflammatory Agents , Antioxidants
2.
Food Res Int ; 188: 114326, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38823825

ABSTRACT

Tormentilla erecta (L.) Raeusch is a widespread plant in Europe and Western Asia. Its rhizomes (Tormentilae rhizoma) are the main ingredient of herbal alcoholic beverages and can be used as a natural preservative in beer production. Apart from its unique taste qualities, therapeutic properties in gastrointestinal tract ailments are attributed to the tincture obtained from Tormentillae rhizoma. The presented research aimed to determine the mutual relationship between the components of Tormentillae tincture, present in popular alcoholic beverages, and intestinal epithelium (Caco-2 cell monolayers). A comprehensive qualitative and quantitative analysis of the tincture was performed, including the determination of condensed and hydrolyzable tannins as well as triterpenoids (UHPLC-DAD-MS/MS). Incubation of the tincture with Caco-2 monolayers has shown that only triterpenes pass through the monolayer, while condensed tannins are mainly bound to the monolayer surface. Ellagic acid derivatives were the only components of the Tormentillae tinctura being metabolized by cell monolayers to the compounds not previously described in the literature, which may be crucial in the treatment of intestinal diseases with inflammatory background.


Subject(s)
Intestinal Mucosa , Rhizome , Humans , Caco-2 Cells , Rhizome/chemistry , Intestinal Mucosa/metabolism , Triterpenes/metabolism , Plant Extracts/metabolism , Plant Extracts/pharmacology , Tandem Mass Spectrometry , Biological Transport , Chromatography, High Pressure Liquid , Alcoholic Beverages/analysis , Proanthocyanidins/metabolism , Hydrolyzable Tannins/metabolism , Ellagic Acid/metabolism
3.
J Agric Food Chem ; 72(23): 13240-13249, 2024 Jun 12.
Article in English | MEDLINE | ID: mdl-38825967

ABSTRACT

Acrolein (ACR), methylglyoxal (MGO), and glyoxal (GO) are a class of reactive carbonyl species (RCS), which play a crucial role in the pathogenesis of chronic and age-related diseases. Here, we explored a new RCS inhibitor (theanine, THE) and investigated its capture capacity on RCS in vivo by human experiments. After proving that theanine could efficiently capture ACR instead of MGO/GO by forming adducts under simulated physiological conditions, we further detected the ACR/MGO/GO adducts of theanine in the human urine samples after consumption of theanine capsules (200 and 400 mg) or green tea (4 cups, containing 200 mg of theanine) by using ultraperformance liquid chromatography-time-of-flight-high-resolution mass spectrometry. Quantitative assays revealed that THE-ACR, THE-2ACR-1, THE-MGO, and THE-GO were formed in a dose-dependent manner in the theanine capsule groups; the maximum value of the adducts of theanine was also tested. Furthermore, besides the RCS adducts of theanine, the RCS adducts of catechins could also be detected in the drinking tea group. Whereas, metabolite profile analysis showed that theanine could better capture RCS produced in the renal metabolic pathway than catechins. Our findings indicated that theanine could reduce RCS in the body in two ways: as a pure component or contained in tea leaves.


Subject(s)
Glutamates , Glyoxal , Pyruvaldehyde , Tea , Humans , Tea/chemistry , Glutamates/metabolism , Glutamates/analysis , Male , Pyruvaldehyde/metabolism , Pyruvaldehyde/chemistry , Glyoxal/metabolism , Glyoxal/chemistry , Adult , Acrolein/metabolism , Acrolein/chemistry , Capsules/chemistry , Camellia sinensis/chemistry , Camellia sinensis/metabolism , Female , Young Adult , Plant Extracts/chemistry , Plant Extracts/metabolism , Plant Extracts/administration & dosage , Chromatography, High Pressure Liquid
4.
Metabolomics ; 20(3): 62, 2024 May 25.
Article in English | MEDLINE | ID: mdl-38796627

ABSTRACT

INTRODUCTION: The chemical classification of Cannabis is typically confined to the cannabinoid content, whilst Cannabis encompasses diverse chemical classes that vary in abundance among all its varieties. Hence, neglecting other chemical classes within Cannabis strains results in a restricted and biased comprehension of elements that may contribute to chemical intricacy and the resultant medicinal qualities of the plant. OBJECTIVES: Thus, herein, we report a computational metabolomics study to elucidate the Cannabis metabolic map beyond the cannabinoids. METHODS: Mass spectrometry-based computational tools were used to mine and evaluate the methanolic leaf and flower extracts of two Cannabis cultivars: Amnesia haze (AMNH) and Royal dutch cheese (RDC). RESULTS: The results revealed the presence of different chemical compound classes including cannabinoids, but extending it to flavonoids and phospholipids at varying distributions across the cultivar plant tissues, where the phenylpropnoid superclass was more abundant in the leaves than in the flowers. Therefore, the two cultivars were differentiated based on the overall chemical content of their plant tissues where AMNH was observed to be more dominant in the flavonoid content while RDC was more dominant in the lipid-like molecules. Additionally, in silico molecular docking studies in combination with biological assay studies indicated the potentially differing anti-cancer properties of the two cultivars resulting from the elucidated chemical profiles. CONCLUSION: These findings highlight distinctive chemical profiles beyond cannabinoids in Cannabis strains. This novel mapping of the metabolomic landscape of Cannabis provides actionable insights into plant biochemistry and justifies selecting certain varieties for medicinal use.


Subject(s)
Cannabis , Metabolomics , Plant Leaves , Cannabis/chemistry , Cannabis/metabolism , Metabolomics/methods , Plant Leaves/metabolism , Plant Leaves/chemistry , Flowers/metabolism , Flowers/chemistry , Plant Extracts/metabolism , Plant Extracts/chemistry , Plant Extracts/pharmacology , Cannabinoids/metabolism , Cannabinoids/analysis , Molecular Docking Simulation , Flavonoids/metabolism , Flavonoids/analysis , Mass Spectrometry/methods
5.
J Agric Food Chem ; 72(20): 11531-11548, 2024 May 22.
Article in English | MEDLINE | ID: mdl-38700894

ABSTRACT

Although recent evidence indicated significant phenol and alkylamide interaction in aqueous solutions, the gastrointestinal digestion influence of the combination remains unclear. This study aims to investigate phenol and alkylamide interaction during in vitro digestion, focusing on bioaccessibility and bioactivity, including α-glucosidase inhibition and cellular antioxidant activity. Additionally, the structural mechanism of phenol and alkylamide interaction during in vitro digestion was explored. The results indicated that the presence of phenols and alkylamides significantly increased or decreased their respective bioaccessibility, depending on the Zanthoxylum varieties. Furthermore, although antagonistic phenol/alkylamide interaction was evident during α-glucosidase inhibition, cellular oxidative stress alleviation, and antioxidant gene transcription upregulation, this effect weakened gradually as digestion progressed. Glycoside bond cleavage and the methylation of phenols as well as alkylamide isomerization and addition were observed during digestion, modifying the hydrogen bonding sites and interaction behavior. This study provided insights into the phenol/alkylamide interaction in the gastrointestinal tract.


Subject(s)
Amides , Antioxidants , Digestion , Glycoside Hydrolase Inhibitors , Plant Extracts , Zanthoxylum , alpha-Glucosidases , Zanthoxylum/chemistry , Zanthoxylum/metabolism , Antioxidants/chemistry , Antioxidants/metabolism , Glycoside Hydrolase Inhibitors/chemistry , Glycoside Hydrolase Inhibitors/metabolism , Glycoside Hydrolase Inhibitors/pharmacology , alpha-Glucosidases/metabolism , alpha-Glucosidases/chemistry , alpha-Glucosidases/genetics , Humans , Amides/chemistry , Amides/metabolism , Amides/pharmacology , Plant Extracts/chemistry , Plant Extracts/metabolism , Plant Extracts/pharmacology , Phenols/chemistry , Phenols/metabolism , Models, Biological , Phenol/metabolism , Phenol/chemistry
6.
Food Chem ; 452: 139574, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38733683

ABSTRACT

Barley leaves (BLs) naturally contained abundant phenolics, most of which are hardly completely released from food matrix during gastrointestinal digestion. Superfine grinding (SFG) and high hydrostatic pressure (HHP) are generally used to treat the functional plants due to their effectiveness to cell wall-breaking and improvement of nutraceutical bioavailability. Thus, this study investigated the synergistic effects of SFG and HHP (100, 300, 500 MPa/20 min) on the bioaccessbility of typical phenolics in BLs during the simulated in-vitro digestion. The results demonstrated that the highest bioaccessbility (40.98%) was found in the ultrafine sample with HHP at 500 MPa. CLSM and SEM confirmed SFG led to microstructurally rapture of BLs. Moreover, the recovery index of ABTS radical scavenging activity and FRAP of HHP-treated ultrafine and fine BLs samples maximumly increased by 53.62% and 9.61%, respectively. This study is expecting to provide the theoretical basis to improve the consumer acceptance of BLs.


Subject(s)
Antioxidants , Digestion , Hordeum , Hydrostatic Pressure , Plant Leaves , Polyphenols , Hordeum/chemistry , Hordeum/metabolism , Plant Leaves/chemistry , Plant Leaves/metabolism , Antioxidants/chemistry , Antioxidants/metabolism , Polyphenols/chemistry , Polyphenols/metabolism , Food Handling , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Extracts/metabolism , Humans
7.
Food Chem ; 452: 139355, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38733679

ABSTRACT

Differently colored foxtail millet (Setaria italica) cultivars were compared regarding their amylose, B-complex vitamin, vitamin E, and phenolic compositions, as well as the bioaccessibility of their phenolics in simulated in vitro digestion. Dark-colored foxtail millets contained more thiamine, pyridoxine, and tocopherols, but less riboflavin, than light-colored ones. Phenolics were more abundant in dark-colored cultivars. Insoluble bound fractions accounted for 75%-83% of the total phenolics, with ferulic acid detected as the most plentiful compound. The major bioaccessible phenolic was free ferulic acid, with 100%-120% bioaccessibility, depending on cultivar, followed by p-coumaric acid and isoferulic acid (50%-80%). These relatively high bioaccessibilities were likely due to the release of soluble conjugated or insoluble bound phenolics during digestion. However, the contents of other free phenolics were largely decreased following in vitro digestion, resulting in low bioaccessibility, which also means that the release from the conjugated and bound fractions was poor.


Subject(s)
Digestion , Phenols , Setaria Plant , Phenols/metabolism , Phenols/chemistry , Phenols/analysis , Setaria Plant/chemistry , Setaria Plant/metabolism , Plant Extracts/chemistry , Plant Extracts/metabolism , Biological Availability , Models, Biological
8.
Food Chem ; 452: 139552, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38733684

ABSTRACT

This study explores the impact of processing techniques on the bioactive composition and antioxidant properties of Bambusa nutans shoots, an underutilized superfood. Boiling resulted in a significant reduction of total phenols (39.52%), flavonoid (8.07%), and tannin (27.77%). Conversely, fermentation increased total phenols (25.92%), tannin (34.72%), and phytosterol (75.39%). Antioxidant activities were notably higher in fermented-shoots, as indicated by DPPH and FRAP assays, and in-vivo experiments demonstrated increased GSH (31.85%) and decreased LPO levels (11.12%) post-administration of fermented-shoots extract. Spectral analysis revealed an increased diversity of compounds in fermented shoots, with GC/MS identifying hexadecane-1-ol in significantly higher proportions and 11 characteristic bands in FTIR spectra. HPLC analysis demonstrated changes in phenolic acids and flavonoid content, with fermentation enhancing 3,4,5-trihydroxybenzoic acid, 4'-hydroxycinnamic acid, benzene-1,2-diol, and luteolin contents. This study underscores the dynamic nature of B. nutans shoots, highlighting the potential for enhanced bioactivity and antioxidant properties through fermentation, offering promising avenues for culinary and pharmaceutical developments.


Subject(s)
Antioxidants , Gas Chromatography-Mass Spectrometry , Plant Extracts , Plant Shoots , Antioxidants/chemistry , Antioxidants/analysis , Antioxidants/metabolism , Chromatography, High Pressure Liquid , Plant Extracts/chemistry , Plant Extracts/metabolism , Plant Shoots/chemistry , Plant Shoots/metabolism , Spectroscopy, Fourier Transform Infrared , Flavonoids/analysis , Flavonoids/chemistry , Flavonoids/metabolism , Phenols/metabolism , Phenols/analysis , Phenols/chemistry , Fermentation , Animals
9.
Food Chem ; 452: 139584, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38735110

ABSTRACT

Rosehips are a prominent source of numerous bioactive compounds. However, despite their extensive potential, the metabolic profiles among different rosehip species have not been fully elucidated. In this study, 523 secondary metabolites from rosehips of 12 Rosa species were identified using ultra-high-performance liquid chromatography-tandem mass spectrometry. They were primarily composed of flavonoids and phenolic acids. A K-means analysis revealed the characteristic metabolites in different rosehips. For example, R. persica contained a more abundant supply of phenolic acids, while R. roxburghii harbored a richer array of terpenoids. A total of 73 key active ingredients were screened from traditional Chinese medicine databases, and they indicated that R. persica is more promising for use in functional foods or health supplements compared with the other fruits. Moreover, a differential analysis identified 47 compounds as potential contributors to the astringent taste of rosehips, including ellagic acid 4-O-glucoside and cadaverine. This study provides valuable information to develop new functional foods of rosehips and improve the quality of their fruits.


Subject(s)
Fruit , Metabolomics , Rosa , Taste , Rosa/chemistry , Rosa/metabolism , Chromatography, High Pressure Liquid , Fruit/chemistry , Fruit/metabolism , Plant Extracts/chemistry , Plant Extracts/metabolism , Plant Extracts/analysis , Tandem Mass Spectrometry , Flavonoids/analysis , Flavonoids/metabolism , Humans , Hydroxybenzoates/analysis , Hydroxybenzoates/metabolism , Flavoring Agents/chemistry , Flavoring Agents/metabolism
10.
Food Chem ; 452: 139606, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38744127

ABSTRACT

In this study, two pectic polysaccharides (PFP-T and PFP-UM) were extracted from fresh passion fruit peels using three-phase partitioning (TPP) and sequential ultrasound-microwave-assisted TPP methods, respectively, and their effects on the in vitro gastrointestinal digestion and fecal fermentation characteristics were examined. The results indicate that gastrointestinal digestion has a minimal effect on the physicochemical and structural characteristics of PFP-T and PFP-UM. However, during in vitro fecal fermentation, both undigested PFP-T and PFP-UM are significantly degraded and utilized by intestinal microorganisms, showing increased the total relative abundance of Firmicutes and Bacteroidota in the intestinal flora. Notably, compared with PFP-UM, PFP-T better promoted the reproduction of beneficial bacteria such as Prevotella, Megasphaera and Dialister, while suppressed the growth of harmful genera including Escherichia-Shigella, producing higher content of short-chain fatty acids. Therefore, our findings suggest that PFP-T derived from passion fruit peel has potential as a dietary supplement for promoting intestinal health.


Subject(s)
Bacteria , Digestion , Fermentation , Fruit , Passiflora , Passiflora/chemistry , Passiflora/metabolism , Fruit/chemistry , Fruit/metabolism , Bacteria/metabolism , Humans , Polysaccharides/metabolism , Polysaccharides/chemistry , Pectins/metabolism , Pectins/chemistry , Feces/microbiology , Feces/chemistry , Gastrointestinal Microbiome , Plant Extracts/chemistry , Plant Extracts/metabolism , Models, Biological
11.
Food Chem ; 452: 139565, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38759437

ABSTRACT

Microgreens constitute natural-based foods with health-promoting properties mediated by the accumulation of glucosinolates (GLs) and phenolic compounds (PCs), although their bioaccessibility may limit their nutritional potential. This work subjected eight Brassicaceae microgreens to in vitro gastrointestinal digestion and large intestine fermentation before the metabolomics profiling of PCs and GLs. The application of multivariate statistics effectively discriminated among species and their interaction with in vitro digestion phases. The flavonoids associated with arugula and the aliphatic GLs related to red cabbage and cauliflower were identified as discriminant markers among microgreen species. The multi-omics integration along in vitro digestion and fermentation predicted bioaccessible markers, featuring potential candidates that may eventually be responsible for these functional foods' nutritional properties. This combined analytical and computational framework provided a promising platform to predict the nutritional metabolome-wide outcome of functional food consumption, as in the case of microgreens.


Subject(s)
Brassicaceae , Glucosinolates , Metabolomics , Polyphenols , Glucosinolates/metabolism , Glucosinolates/analysis , Glucosinolates/chemistry , Polyphenols/metabolism , Polyphenols/chemistry , Polyphenols/analysis , Brassicaceae/metabolism , Brassicaceae/chemistry , Digestion , Humans , Chemometrics , Plant Extracts/metabolism , Plant Extracts/chemistry
12.
J Agric Food Chem ; 72(20): 11549-11560, 2024 May 22.
Article in English | MEDLINE | ID: mdl-38718199

ABSTRACT

Corinthian currants are dried fruits produced from Vitis vinifera L. var. Apyrena grape. This study investigated the distribution of phenolic compounds in male Wistar rat livers following two distinct Corinthian currant long-term dietary intake protocols (3 and 10% w/w). Method optimization, comparing fresh and lyophilized tissues, achieved satisfactory recoveries (>70%) for most analytes. Enzymatic hydrolysis conditions (37 °C, pH 5.0) minimally affected phenolics, but enzyme addition showed diverse effects. Hydrolyzed lyophilized liver tissue from rats consuming Corinthian currants (3 and 10% w/w) exhibited elevated levels of isorhamnetin (20.62 ± 2.27 ng/g tissue and 33.80 ± 1.38 ng/g tissue, respectively), along with similar effects for kaempferol, quercetin, and chrysin after prolonged Corinthian currant intake. This suggests their presence as phase II metabolites in the fasting-state liver. This study is the first to explore phenolic accumulation in rat liver, simulating real conditions of dried fruit consumption, as seen herein with Corinthian currant.


Subject(s)
Flavonoids , Fruit , Liver , Rats, Wistar , Tandem Mass Spectrometry , Vitis , Animals , Flavonoids/metabolism , Flavonoids/chemistry , Male , Rats , Vitis/chemistry , Vitis/metabolism , Liver/metabolism , Liver/chemistry , Fruit/chemistry , Fruit/metabolism , Hydroxybenzoates/metabolism , Hydroxybenzoates/analysis , Hydroxybenzoates/chemistry , Plant Extracts/chemistry , Plant Extracts/metabolism , Plant Extracts/administration & dosage , Chromatography, High Pressure Liquid
13.
J Agric Food Chem ; 72(20): 11493-11502, 2024 May 22.
Article in English | MEDLINE | ID: mdl-38738816

ABSTRACT

Cacumen platycladi (CP) is a frequently used traditional Chinese medicine to treat hair loss. In this study, CP fermented by Lactiplantibacillus plantarum CCFM1348 increased the proliferation of human dermal papilla cells. In an in vivo assay, compared to nonfermented CP, postbiotics (fermented CP) and synbiotics (live bacteria with nonfermented CP) promoted hair growth in mice. The Wnt/ß-catenin signaling pathway plays crucial roles in the development of hair follicles, including growth cycle restart and maintenance. Both postbiotics and synbiotics upregulated ß-catenin, a major factor of the Wnt/ß-catenin signaling pathway. Postbiotics and synbiotics also increased the vascular endothelial growth factor expression and decreased the BAX/Bcl2 ratio in the dorsal skin of mice. These results suggest that fermented CP by L. plantarum CCFM1348 may promote hair growth through regulating the Wnt/ß-catenin signaling pathway, promoting the expression of growth factors and reducing apoptosis.


Subject(s)
Hair , Wnt Signaling Pathway , Animals , Mice , Hair/metabolism , Hair/growth & development , Hair/chemistry , Humans , Wnt Signaling Pathway/drug effects , Biotransformation , Fermentation , beta Catenin/metabolism , beta Catenin/genetics , Male , Plant Extracts/metabolism , Plant Extracts/pharmacology , Plant Extracts/chemistry , Hair Follicle/metabolism , Hair Follicle/growth & development , Cell Proliferation/drug effects , Apoptosis/drug effects , Lactobacillus plantarum/metabolism , Lactobacillus plantarum/growth & development
14.
Food Chem ; 453: 139628, 2024 Sep 30.
Article in English | MEDLINE | ID: mdl-38761731

ABSTRACT

Umami taste is a key criteria of green tea quality evaluation. The aim of this study was to comprehensively explore the key umami taste contributors in Longjing tea. The taste and molecular profiles of 36 Longjing green tea infusions were characterized by sensory quantitative descriptive analysis and LC-MS based metabolomics, respectively. By uni-/multi-variate statistical analysis, 84 differential compounds were screened among tea infusions with varied umami perceptions. Among them, 17 substances were identified as candidate umami-enhancing compounds, which showed significant positive correlations with umami intensities. Their natural concentrations were accurately quantified, and their umami taste-modifying effects were further investigated by taste addition into glutamic acid solution. Glutamic acid, aspartic acid, glutamine, theanine, phenylalanine, histidine, theogallin, galloylglucose, 1,2,6-trigalloylglucose significantly enhanced the umami taste. This study uncovered for the first time of some bitter amino acids and galloylglucose homologous series as important umami-enhancers, which provided a novel perspective into the tea taste.


Subject(s)
Camellia sinensis , Metabolomics , Taste , Tea , Tea/chemistry , Humans , Camellia sinensis/chemistry , Camellia sinensis/metabolism , Flavoring Agents/chemistry , Flavoring Agents/metabolism , Male , Adult , Mass Spectrometry , Female , Plant Extracts/chemistry , Plant Extracts/metabolism , Plant Extracts/analysis , Chromatography, High Pressure Liquid
15.
Food Chem ; 453: 139659, 2024 Sep 30.
Article in English | MEDLINE | ID: mdl-38776792

ABSTRACT

There is a lack of research on how Tibetan kefir grains fermentation alters the physicochemical properties and biological activity of Lycium barbarum pulp polysaccharides, despite some reports that fermentation can affect the structure and activity of plant polysaccharides. This study demonstrated that, through fermentation, the molecular weight of polysaccharides decreased from 25.33 to 15.11 kg/mol while the contents of total sugar and uronic acid increased by 19.11% and 40.38%, respectively. Furthermore, after fermentation, the polysaccharides exhibited an uneven and rough surface along with a reduced number of branched chains and triple helix structures. Tibetan kefir grains fermentation enhanced the antioxidant activity of polysaccharides, which may be attributed to an increase in arabinose, galactose, and uronic acid content and a decrease in polysaccharide molecular weight. This research offers an alternative viewpoint on the potential application of Tibetan kefir grains-fermented Lycium barbarum pulp polysaccharides in functional foods.


Subject(s)
Antioxidants , Fermentation , Kefir , Lycium , Polysaccharides , Lycium/chemistry , Lycium/metabolism , Antioxidants/chemistry , Antioxidants/metabolism , Antioxidants/pharmacology , Kefir/microbiology , Kefir/analysis , Polysaccharides/chemistry , Polysaccharides/metabolism , Polysaccharides/pharmacology , Molecular Weight , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Extracts/metabolism , Drugs, Chinese Herbal
16.
Biochim Biophys Acta Mol Cell Biol Lipids ; 1869(6): 159505, 2024 Aug.
Article in English | MEDLINE | ID: mdl-38729236

ABSTRACT

Tylophora indica (Burm f.) Merrill, belong to family Asclepiadaceae, is considered to be a natural remedy with high medicinal benefits. The objective of this work is to assess the metabolomic profile of T. indica leaves enriched in alkaloids, as well as to evaluate the in vitro cytotoxicity of these leaves using the MTT assay on human breast MCF-7 and liver HepG2 cancer cell lines. Dried leaves of T. indica were extracted by sonication, using methanol containing 2 % (v/v) of acetic acid and obtained fraction was characterized by HPTLC and UPLC-MS. The UPLC-MS study yielded a preliminary identification of 32 metabolites, with tylophorine, tylophorine B, tylophorinine, and tylophorinidine being the predominant metabolites. The cytotoxicity of the extract of T. indica was evaluated on HepG2 and MCF-7 cell lines, yielding inhibitory concentration (IC50) values of 75.71 µg/mL and 69.60 µg/mL, respectively. Data suggested that the phytochemical screening clearly showed presence of numerous secondary metabolites with moderate cytotoxic efficacy. In conclusion, the future prospects of T. indica appear promising for the advancement of phytopharmaceutical-based anticancer medications, as well as for the design of contemporary pharmaceuticals in the field of cancer chemotherapy.


Subject(s)
Alkaloids , Metabolomics , Plant Extracts , Plant Leaves , Tylophora , Humans , Plant Leaves/metabolism , Plant Leaves/chemistry , Alkaloids/metabolism , Alkaloids/pharmacology , Alkaloids/chemistry , Hep G2 Cells , Metabolomics/methods , MCF-7 Cells , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Extracts/metabolism , Tylophora/metabolism , Tylophora/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/metabolism
17.
Microbiol Spectr ; 12(6): e0403123, 2024 Jun 04.
Article in English | MEDLINE | ID: mdl-38738925

ABSTRACT

STW 5, a blend of nine medicinal plant extracts, exhibits promising efficacy in treating functional gastrointestinal disorders, notably irritable bowel syndrome (IBS). Nonetheless, its effects on the gastrointestinal microbiome and the role of microbiota on the conversion of its constituents are still largely unexplored. This study employed an experimental ex vivo model to investigate STW 5's differential effects on fecal microbial communities and metabolite production in samples from individuals with and without IBS. Using 560 fecal microcosms (IBS patients, n = 6; healthy controls, n = 10), we evaluated the influence of pre-digested STW 5 and controls on microbial and metabolite composition at time points 0, 0.5, 4, and 24 h. Our findings demonstrate the potential of this ex vivo platform to analyze herbal medicine turnover within 4 h with minimal microbiome shifts due to abiotic factors. While only minor taxonomic disparities were noted between IBS- and non-IBS samples and upon treatment with STW 5, rapid metabolic turnover of STW 5 components into specific degradation products, such as 18ß-glycyrrhetinic acid, davidigenin, herniarin, 3-(3-hydroxyphenyl)propanoic acid, and 3-(2-hydroxy-4-methoxyphenyl)propanoic acid occurred. For davidigenin, 3-(3-hydroxyphenyl)propanoic acid and 18ß-glycyrrhetinic acid, anti-inflammatory, cytoprotective, or spasmolytic activities have been previously described. Notably, the microbiome-driven metabolic transformation did not induce a global microbiome shift, and the detected metabolites were minimally linked to specific taxa. Observed biotransformations were independent of IBS diagnosis, suggesting potential benefits for IBS patients from biotransformation products of STW 5. IMPORTANCE: STW 5 is an herbal medicinal product with proven clinical efficacy in the treatment of functional gastrointestinal disorders, like functional dyspepsia and irritable bowel syndrome (IBS). The effects of STW 5 on fecal microbial communities and metabolite production effects have been studied in an experimental model with fecal samples from individuals with and without IBS. While only minor taxonomic disparities were noted between IBS- and non-IBS samples and upon treatment with STW 5, rapid metabolic turnover of STW 5 components into specific degradation products with reported anti-inflammatory, cytoprotective, or spasmolytic activities was observed, which may be relevant for the pharmacological activity of STW 5.


Subject(s)
Biotransformation , Feces , Gastrointestinal Microbiome , Irritable Bowel Syndrome , Plant Extracts , Irritable Bowel Syndrome/microbiology , Irritable Bowel Syndrome/metabolism , Irritable Bowel Syndrome/drug therapy , Gastrointestinal Microbiome/drug effects , Humans , Feces/microbiology , Adult , Plant Extracts/metabolism , Plant Extracts/pharmacology , Male , Female , Bacteria/metabolism , Bacteria/classification , Bacteria/isolation & purification , Bacteria/drug effects , Bacteria/genetics , Middle Aged , Plants, Medicinal/microbiology , Plants, Medicinal/chemistry
18.
J Agric Food Chem ; 72(23): 12975-12987, 2024 Jun 12.
Article in English | MEDLINE | ID: mdl-38807047

ABSTRACT

This study discovered the impact of high-tunnel (i.e., unheated greenhouse) and open-field production on two industrial hemp cultivars (SB1 and CJ2) over their yield parameters, cannabinoid development, and volatile profiles. Development of neutral cannabinoids (CBD, THC, and CBC), acidic cannabinoids (CBDA, THCA, and CBCA), and total cannabinoids during floral maturation were investigated. The volatile profiles of hemp flowers were holistically compared via HS-SPME-GC/MS. Findings indicated a high tunnel as an efficient practice for achieving greater total weight, stem number, and caliper, especially in the SB1 cultivar. Harvesting high-tunnel-grown SB1 cultivars during early flower maturation could obtain a high CBD yield while complying with THC regulations. Considering the volatile profiles, hemp flowers mainly consisted of mono- and sesquiterpenoids, as well as oxygenated mono- and sesquiterpenoids. Volatile analysis revealed the substantial impact of cultivars on the volatile profile compared to the production systems.


Subject(s)
Cannabinoids , Cannabis , Gas Chromatography-Mass Spectrometry , Inflorescence , Volatile Organic Compounds , Cannabis/chemistry , Cannabis/growth & development , Cannabis/metabolism , Cannabinoids/analysis , Cannabinoids/metabolism , Volatile Organic Compounds/chemistry , Volatile Organic Compounds/metabolism , Volatile Organic Compounds/analysis , Inflorescence/chemistry , Inflorescence/growth & development , Inflorescence/metabolism , Flowers/chemistry , Flowers/growth & development , Flowers/metabolism , Plant Extracts/chemistry , Plant Extracts/metabolism
19.
Braz J Microbiol ; 55(2): 1655-1667, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38635155

ABSTRACT

The health benefits of functional foods are associated with consumer interest and have supported the growth of the market for these types of foods, with emphasis on the development of new formulations based on plant extracts. Therefore, the present study aimed to characterize a symbiotic preparation based on water-soluble soy extract, supplemented with inulin and xylitol and fermented by Lactiplantibacillus plantarum ATCC 8014. Regarding nutritional issues, the symbiotic formulation can be considered a source of fiber (2 g/100 mL) and proteins (2.6 g/100 mL), and it also has a low-fat content and low caloric value. This formulation, in terms of microbiological aspects, remained adequate to legal standards after storage for 60 days under refrigeration and also presented an adequate quantity of the aforementioned probiotic strain, corresponding to 9.11 Log CFU.mL-1. These viable L. plantarum cells proved to be resistant to simulated human gastrointestinal tract conditions, reaching the intestine at high cell concentrations of 7.95 Log CFU.mL-1 after 60 days of refrigeration. Regarding sensory evaluation, the formulation showed good acceptance, presenting an average overall impression score of 6.98, 5.98, and 5.16, for control samples stored for 30 and 60 days under refrigeration, respectively. These results demonstrate that water-soluble soy extract is a suitable matrix for fermentation involving L. plantarum ATCC 8014, supporting and providing data on the first steps towards the development of a symbiotic functional food, targeting consumers who have restrictions regarding the consumption of products of animal origin, diabetics, and individuals under calorie restrictions.


Subject(s)
Fermentation , Glycine max , Lactobacillus plantarum , Probiotics , Glycine max/microbiology , Glycine max/chemistry , Probiotics/metabolism , Humans , Lactobacillus plantarum/metabolism , Lactobacillus plantarum/growth & development , Plant Extracts/chemistry , Plant Extracts/metabolism , Beverages/microbiology , Beverages/analysis
20.
Food Chem ; 449: 139183, 2024 Aug 15.
Article in English | MEDLINE | ID: mdl-38604028

ABSTRACT

Tartary buckwheat, celebrated as the "king of grains" for its flavonoid and phenolic acid richness, has health-promoting properties. Despite significant morphological and metabolic variations in mature achenes, research on their developmental process is limited. Utilizing Liquid chromatography-mass spectrometry and atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry imaging, we conducted spatial-temporal metabolomics on two cultivars during achene development. Metabolic profiles including 17 phenolic acids and 83 flavonoids are influenced by both varietal distinctions and developmental intricacies. Notably, flavonols, as major flavonoids, accumulated with achene ripening and showed a tissue-specific distribution. Specifically, flavonol glycosides and aglycones concentrated in the embryo, while methylated flavonols and procyanidins in the hull. Black achenes at the green achene stage have higher bioactive compounds and enhanced antioxidant capacity. These findings provide insights into spatial and temporal characteristics of metabolites in Tartary buckwheat achenes and serve as a theoretical guide for selecting optimal resources for food production.


Subject(s)
Fagopyrum , Metabolomics , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Fagopyrum/chemistry , Fagopyrum/growth & development , Fagopyrum/metabolism , Flavonoids/metabolism , Flavonoids/chemistry , Flavonoids/analysis , Chromatography, High Pressure Liquid , Plant Extracts/metabolism , Plant Extracts/chemistry , Seeds/chemistry , Seeds/growth & development , Seeds/metabolism , Liquid Chromatography-Mass Spectrometry
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