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1.
Arq. bras. med. vet. zootec. (Online) ; 71(5): 1565-1570, set.-out. 2019. ilus
Article in Portuguese | VETINDEX, LILACS | ID: biblio-1038641

ABSTRACT

Campylobacter jejuni é o principal causador de gastroenterite bacteriana aguda, e a carne de frango tem se mostrado uma importante fonte de transmissão. Este microrganismo é de difícil isolamento e os métodos convencionais muitas vezes não são eficientes, podendo levar a resultados errôneos. O objetivo deste trabalho foi desenvolver e testar a técnica de separação imunomagnética (IMS) na detecção de C. jejuni em produtos de frango. Micropartículas magnéticas ligadas a anticorpos policlonais anti-C. jejuni foram utilizadas para concentrar C. jejuni antes da semeadura em ágar. O protocolo foi comparado com o método convencional. C. jejuni foi recuperado do alimento experimentalmente contaminado por ambos os métodos, entretanto, quando foi usada a IMS, a presença de microrganismos contaminantes nos meios de cultura foi menor. C. jejuni foi isolado de 7% das amostras de alimento naturalmente contaminadas, usando IMS, e de 3% pelo método convencional. C. coli foi isolado de uma amostra pelo método convencional, mas não foi detectado pelo protocolo com IMS. A técnica de IMS pode ser usada para isolamento de C. jejuni de alimentos, oferecendo a vantagem de detectar em amostras o microrganismo cujo isolamento não é obtido por meio do método convencional.(AU)


Campylobacter jejuni is the main cause of acute bacterial gastroenteritis and chicken meat has shown to be an important source of infection. This microorganism is difficult to isolate and the conventional methods are often inefficient and may lead to erroneous results. This study aimed at developing and testing the technique of immunomagnetic separation (IMS) in the detection of C. jejuni in chicken products. Microparticles magnetically connected anti-C. jejuni polyclonal antibodies were used to concentrate C. jejuni before agar seeding. The protocol was compared with the conventional method. C. jejuni was recovered from experimentally contaminated food for both methods, however, when the IMS was used, the presence of contaminating microorganisms in the means of culture was smaller. C. jejuni was isolated from 7% of samples of food naturally contaminated, using IMS, and 3% by conventional method. C. coli was isolated from a sample by conventional method, but it was not detected by protocol with IMS. The IMS technique can be used for isolation of C. jejuni in food, offering the advantage of detecting the microorganism in samples from which the isolation is not obtained with the use of the conventional method.(AU)


Subject(s)
Poultry Products/microbiology , Poultry Products/toxicity , Campylobacter Infections/microbiology , Campylobacter jejuni/isolation & purification , Chickens
2.
Hig. aliment ; 33(288/289): 2476-2480, abr.-maio 2019. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1482243

ABSTRACT

O presente estudo tem como objetivo descrever a distribuição de probabilidades da temperatura de cada etapa da cadeia produtiva de carne de frango resfriada produzida sob inspeção federal no Brasil. Os resultados gerados geram subsídio para realização de uma Análise de Risco de salmonelose pelo consumo de carne de frango no país. Foram compilados registros de temperatura e os dados foram ajustados às distribuições de probabilidade utilizando o software @Risk. Nas etapas de abatedouro-frigorífico, centro de distribuição, serviço de alimentação e supermercado a média de temperatura foi inferior a 5,5 ºC, demostrando sucesso na manutenção da cadeia de frio. A distribuição de probabilidade Logistic demonstrou melhor ajuste para descrever as etapas de processamento. Os dados de distribuição de probabilidade gerados poderão ser utilizados em modelos matemáticos e de distribuição para predizer o crescimento microbiano em alimentos expostos a diferentes temperaturas.


Subject(s)
Perishable Foods , Abattoirs , Risk Assessment , Food Microbiology , Poultry Products/analysis , Poultry Products/microbiology , Poultry Products/toxicity , Salmonella/isolation & purification , Temperature , Chickens , Salmonella Infections/prevention & control
3.
Arq. bras. med. vet. zootec ; 66(6): 1917-1923, 12/2014. tab
Article in Portuguese | LILACS | ID: lil-735780

ABSTRACT

A enrofloxacina é um dos antimicrobianos mais utilizados na avicultura industrial, e a deposição de resíduos em produtos avícolas, como os ovos, são de grande importância para a saúde pública. Na legislação brasileira não existe padronização do período de carência para o seu uso na produção avícola e não há Limite Máximo de Resíduo (LMR) fixado para enrofloxacina em ovos. Neste estudo, foi utilizado o kit de ELISA comercial (Bioo Scientific(r)) e a LC-MS/MS na pesquisa de enrofloxacina em ovos de 30 galinhas tratadas previamente via água de bebida, com 10mg/kg de enrofloxacina, durante cinco dias. Seis ovos foram coletados diariamente e analisados durante o tratamento e após a sua suspensão, durante 15 dias. A deposição de resíduos obteve níveis máximos no quinto dia de tratamento das aves, declinando gradativamente até não ser detectada a partir do nono dia de suspensão do tratamento. Considerando como base o LMR de 100µg/kg fixado pelo Brasil para tecidos comestíveis de aves e pela União Europeia para músculo, gordura e pele, após seis dias de suspensão do tratamento, os níveis de resíduos foram inferiores a esse limite, tendo como médias 37,43µg/kg na LC-MS/MS e 14,731µg/kg no ELISA. Dentro das condições deste estudo, um período de carência de seis dias seria mais adequado para utilização dos ovos para consumo humano. Foram detectados valores de resíduos nos ovos menores no ELISA em relação à LC-MS/MS para a mesma amostra, mas os dois métodos apresentaram concordância estatística entre si. A LC-MS/MS é o teste recomendado pela legislação brasileira para a análise de resíduos em alimentos; entretanto, pelos resultados obtidos, o kit de ELISA utilizado também pode ser aplicado na detecção de resíduos de enrofloxacina em ovos, com as vantagens de rapidez e simplicidade...


Enrofloxacin is one of the most used antibiotics in the poultry industry and the deposition of residues in poultry products, such as eggs, are of great concern to public health. In Brazilian law there is no standard withdrawal period for enrofloxacin in eggs and there is no Maximum Residue Limit (MRL) established for this antimicrobial in eggs. In this study, (Bioo Scientific(r)) commercial ELISA kit and LC-MS/MS were used to investigate enrofloxacin in eggs of 30 hens pretreated via drinking water at 10mg/kg of enrofloxacin for five days. Six eggs were collected daily and analyzed during treatment and after the end of treatment, for 15 days. Residues obtained maximum levels on the fifth day of treatment, declined gradually and were no longer detected from the ninth day to the end of treatment. Based on the MRL of 100mg/kg established for edible tissues of poultry by Brazillian law and for muscle, fat and skin, by the European Union, after six days of treatment withdrawal, the residue levels were below that limit, with the average of 37.43mg/kg in LC-MS/MS and 14.731mg/kg in ELISA. Within the conditions of this study, a withdrawal period of six days would be more appropriate to use the eggs for human consumption. The values obtained by ELISA for residues in eggs were lower than those obtained in LC-MS/MS for the same sample, however both methods showed statistical agreement. LC-MS/MS is the recommended method by Brazilian legislation for analysis of residues in food, however, according to the results the ELISA kit used can also be applied to the detection of enrofloxacin residues in eggs, with the advantages of speed and simplicity...


Subject(s)
Animals , Eggs/analysis , Eggs/toxicity , Poultry Products/analysis , Poultry Products/toxicity , Agro Toxic Maximum Allowable Limit on Food , Poultry
4.
Food Chem Toxicol ; 46(2): 480-9, 2008 Feb.
Article in English | MEDLINE | ID: mdl-17919801

ABSTRACT

Chicken breast extract (CBEX) is obtained via hot water extraction of chicken breast and contains among its primary constituents carnosine and anserine, which are histidine-containing dipeptides present in the muscle tissues of most vertebrate species. Dietary intake of CBEX has been previously shown to buffer hydrogen ions formed during high-intensity exercise in human skeletal muscle cells, thereby inhibiting a decrease in muscle cell pH and subsequent muscle fatigue. The objective of this paper is to report the results of safety studies completed on CBEX. CBEX was determined to have an oral LD(50) value of more than 6000 mg/kg body weight in rats. Gavage doses of 500 or 2000 mg CBEX/kg body weight/day administered to rats for 90 days produced no toxicologically significant, dose-related, differences between control and treated animals with respect to body weight gain, food consumption, behavioral effects, hematological and clinical chemistry parameters, absolute and relative organ weights, or gross and microscopic findings. In the presence or absence of metabolic activation, CBEX exerted no mutagenic activity in the Ames assay conducted in various strains of Salmonella typhimurium and Escherichia coli. The results of these studies support the safety of CBEX as a potential dietary source of carnosine and anserine.


Subject(s)
Anserine/toxicity , Carnosine/toxicity , Poultry Products/toxicity , Animals , Anserine/analysis , Anserine/pharmacology , Carnosine/analysis , Carnosine/pharmacology , Chickens , Dose-Response Relationship, Drug , Escherichia coli/drug effects , Female , Food , Lethal Dose 50 , Male , Mutagenicity Tests , Organ Size/drug effects , Poultry Products/analysis , Rats , Rats, Wistar , Safety , Salmonella typhimurium/drug effects
5.
Food Addit Contam ; 22(10): 920-37, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16227176

ABSTRACT

One important aspect within the European Union's public healthcare is the exposure of consumers to undesirable chemicals in the diet. Food-contact materials (FCM) are one potential contamination source and therefore of particular interest for food exposure assessment. On the other hand, scientific investigations concerning the migration potential and behaviour of food-packaging materials have demonstrated that diffusion in and migration from FCM are foreseeable physical and, in principle, mathematically describable processes. Because of this situation and the current state-of-the-art in migration science, a research project was initiated within the 5th Framework Programme of the European Commission. This project, with the acronym 'FOODMIGROSURE' (European Union Contract No. 'QLK1-CT2002-2390') started on 1 March 2003, was due to last 3 years and had the participation of nine European project partners (see the project website: www.foodmigrosure.org). The aim of the project was to extend currently existing migration models (which have been demonstrated to be applicable for less complex matrices such as food simulants) to foodstuffs themselves. In this way, the project aims to provide a novel and economic tool for estimation of consumer exposure to chemicals migrating from food-contact plastic materials under any actual contact conditions. In addition, the project aims to increase knowledge of the mechanisms of diffusion of organic compounds in foodstuffs and provide data on the partitioning effects between FCM and foods. Today the latter aspect is increasingly regarded as a fundamental influence parameter for migration into foods. Based on the project achievements, a much better scientific basis is available to allow scientifically appropriate amendments of European Union Directive 85/572/EEC as well as to support further developments with the so-called Plastics Directive 2002/72/EC. The paper introduces the project and presents an overview of the project work progress.


Subject(s)
Food Contamination , Food Packaging , Plastics/toxicity , Attitude to Health , Beverages/analysis , Beverages/toxicity , Butadienes/analysis , Butadienes/toxicity , Carcinogens/analysis , Carcinogens/toxicity , Cheese/analysis , Cheese/toxicity , Citrus sinensis , Computer Simulation , Cyclohexenes , Diet , Environmental Exposure/adverse effects , Food Analysis , Humans , Kinetics , Limonene , Models, Theoretical , Molecular Weight , Naphthalenes/analysis , Naphthalenes/toxicity , Poultry Products/analysis , Poultry Products/toxicity , Safety , Styrene/analysis , Styrene/toxicity , Terpenes/analysis , Terpenes/toxicity
6.
Rev. toxicol ; 20(3): 187-192, sept.-dic. 2003. tab, ilus
Article in Es | IBECS | ID: ibc-28967

ABSTRACT

Durante los últimos años se han desarrollado varias alternativas con el objetivo de reemplazar la prueba de irritación ocular en el conejo. El objetivo de este trabajo fue comparar la utilidad de tres protocolos diferentes de la prueba de la membrana corioalantoidea del huevo de gallina, descrita en una base de datos establecida de técnicas de Toxicología in vitro, evaluando la irritación potencial de diez sustancias químicas y formulaciones. Se compararon los datos con aquellos obtenidos en la prueba animal tradicional. Se halló una buena correlación con los datos del test in vivo para dos de los protocolos usados, en que tres sustancias fueron clasificadas como no irritantes y siete como irritantes en alguna magnitud. Sin embargo, se encontraron seis falsos negativos con la tercera prueba usada. Se discuten posibles causas para estos, resultados. De las tres técnicas estudiadas la mejor es la que se basa en la utilización de azul de tripano ya que se trata de una técnica más rápida, de más fácil realización y con un resultado más fiable al evaluar los daños ocasionados en la membrana corioalantoidea de una forma objetiva y cuantificable (AU)


Subject(s)
Animals , Rabbits , Ovum , Eggs/analysis , Eggs/adverse effects , Irritants/adverse effects , Irritants/toxicity , Ophthalmic Solutions/adverse effects , Ophthalmic Solutions/toxicity , Sensitivity and Specificity , Poultry Products/adverse effects , Poultry Products/toxicity , Biological Assay/methods , Biological Assay , Clinical Protocols/classification , Clinical Protocols/standards , Eye Manifestations
8.
Bol. Centro Pesqui. Process. Aliment ; 4(1): 21-44, jan.-jun. 1986. ilus, tab
Article in Portuguese | LILACS | ID: lil-108824

ABSTRACT

O presente trabalho teve por objetivo precípuo avaliar o grau de contaminaçao dos frangos comercializados no Grande Rio por metais pesados, determinando-se o seu conteúdo de chumbo e cádmio. Simultaneamente, verificou-se a possibilidade do uso, para fins de análise de rotina em produtos cárneos, de um espectrofotômetro de absorçao atômica com menor resoluçao e, assim, de preço mais acessível, bem como do emprego da mineralizaçao da matéria orgânica por via seca, com auxílio de coadjuvantes, a fim de evitar o uso de ácidos minerais de alta pureza, importados de elevado custo e de difícil obtençao. Os resultados mostram que as aves analisadas nao continham teores de Pb e Cd detectáveis pelo método empregado, isto é, nao continham teores acima do "ruído" de 0,4 ppm para Pb e 0,04 ppm para Cd. (Valores até 8 e 1 ppm respectivamente, sao permitidos pela


Subject(s)
Animals , Chickens , Poultry Products/toxicity , Liver
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